• Journal of The Korean Society of Grassland and Forage Science
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• v.29 no.3
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• pp.179-186
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• 2009

This study was conducted to evaluate yield and forage quality of domestic silage corn hybrids at different planting dates. Days to silking of Suwon169 and P3394 were 62 days, respectively, the shortest among corn hybrids and Gangdaok was the longest. Cheonganok was more resistant to lodging than other hybrids. Kwangpyeongok and Gangdaok were more excellent to stay-green than other hybrids. Ear ratios to total dry matter of other domestic hybrids except Gangdaok were similar with those of DK697 and P3394. Ear ratios to total dry matter were similar in planting dates, May 3 and May 21 with 46% and 45% but June 11, low with 41%. Fresh yield of Gangdaok was the highest of all hybrids and other hybrids except Gangdaok were similar. Dry matter (DM) yields of domestic hybrids except Cheonganok were similar to those of DK697 and P3394. Total digestible nutrients (TDN) yields of domestic hybrids were no significant difference compared with those of DK697 and P3394. Fresh yield, DM yield and TDN yield of all hybrids were similar in planting dates, May 3 and May 21 but those of all hybrids were reduced in planting date, June 11 compared with May 3 and May 21. All hybrids were no significant difference in acid detergent fiber (ADF) which had values ranging from $44.2{\sim}46.2%$. All hybrids were no significant difference in neutral detergent fiber (NDF) which had values ranging from $58.1{\sim}59.9%$. There were no significant differences in ADF and NDF according to planting dates. Relative feed value (RFV) of P3394 was the lowest of all hybrids. Other hybrids except P3394 had values ranging from $84.1{\sim}85.0$ and was no significant difference in RFV. It is concluded that domestic hybrids tested in this study have high forage quality as well as high production similar to those of imported corn hybrids.

• Animal Bioscience
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• v.34 no.4
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• pp.642-651
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• 2021

Objective: This study aimed to determine the effects of different roughages in total mixed ration (TMR) inoculated with or without coculture of Lactobacillus acidophilus (L. acidophilus) and Bacillus subtilis (B. subtilis) on in vitro rumen fermentation and microbial population. Methods: Three TMRs formulations composed of different forages were used and each TMR was grouped into two treatments: non-fermented TMR and fermented TMR (F-TMR) (inoculated with coculture of L. acidophilus and B. subtilis). After fermentation, the fermentation, chemical and microbial profile of the TMRs were determined. The treatments were used for in vitro rumen fermentation to determine total gas production, pH, ammonianitrogen (NH3-N), and volatile fatty acids (VFA). Microbial populations were determined by quantitative real-time polymerase chain reaction (PCR). All data were analyzed as a 3×2 factorial arrangement design using the MIXED procedure of Statistical Analysis Systems. Results: Changes in the fermentation (pH, lactate, acetate, propionate, and NH3-N) and chemical composition (moisture, crude protein, crude fiber, and ash) were observed. For in vitro rumen fermentation, lower rumen pH, higher acetate, propionate, and total VFA content were observed in the F-TMR group after 24 h incubation (p<0.05). F-TMR group had higher acetate concentration compared with the non-fermented group. Total VFA was highest (p<0.05) in F-TMR containing combined forage of domestic and imported source (F-CF) and F-TMR containing Italian ryegrass silage and corn silage (F-IRS-CS) than that of TMR diet containing oat, timothy, and alfalfa hay. The microbial population was not affected by the different TMR diets. Conclusion: The use of Italian ryegrass silage and corn silage, as well as the inoculation of coculture of L. acidophilus and B. subtilis, in the TMR caused changes in the pH, lactate and acetate concentrations, and chemical composition of experimental diets. In addition, F-TMR composed with Italian ryegrass silage and corn silage altered ruminal pH and VFA concentrations during in vitro rumen fermentation experiment.

• Journal of The Korean Society of Grassland and Forage Science
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• v.40 no.2
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• pp.98-105
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• 2020

This experiment was conducted to a comparison of the productivity according to variety and forage quality by plant parts of imported silage corn (Zea mays, L) in Pyeongchang. The corns evaluated in this experiment were 8 varieties (P1184, P1151, P1194, P1543, P1345, P1429, P1443, and P2105) introduced from the United States, Pioneer Hybrid Co. The harvested corn was divided into 5 plant parts (leaf, stem, cob, husk, and grain), and the ratio of each part was calculated using dry weight and the feed value was analyzed. The emergence rate of corn was generally good except for the P1151 and P2105 varieties. The average tasseling date was July 24^th and the silking date was July 27^th, but the P2105 variety was late to July 28^th and August 1^st, and the remaining varieties were similar. P1345 was the highest (289 and 123 cm), and P1151 varieties were the lowest (267 and 101 cm) in the plant and ear height. Disease resistance was low in P1184, P1443 and P1429, and P1197 and P1345 were high. In the case of stover, the dry matter (DM) content was the lowest at 19.6% in the P1151 and the highest at 24.9% in the P1429. DM content of ear was the highest in the P2105 (55.5%), and P1184 (54.2%) and P1345 (54.3%) were also significantly higher (p<0.05). The DM yield of stover of P2105, P1429 and P1194 varieties was significantly higher (p<0.05), and ear yield of P2105, P1345 and P1443 was higher. The proportions of each part of plants (leaf, stem, cob, husk, and grain) divided by 5 was high, with 50-60% of the ear(grain+cob) ratio. The ratio of husk and cob was roughly similar, and the leaf and stem part showed a ratio of about 20%. The crude protein (CP) content was highest in leaf, followed by grain. The CP content of the stem was the lowest, and the husk was not significantly different among the varieties (p>0.05). The acid detergent fiber (ADF) content was similar to the rest parts except grain, but the leaf part tended to be lower, and other parts except the stem and leaf showed no significant difference between varieties (p>0.05). There was no significant difference in NDF (neutral detergent fiber) content in husk, but there was a difference between varieties in other parts (p<0.05). In addition, there was a special difference by plant parts for each variety, P2015 on the stem, P1197 on the leaf, P1151 on the cob, P1197 on the husk, and P1197 on the grains with high NDF content. IVDMD (in vitro dry matter digestibility) was not significantly different between stems and grains, but there was a difference between varieties in cobs and husks. According to the results, DM yield of P2105 variety was the best in the experiment, and the ratio of grain was excellent in P1543 and P1345. In addition, it was found that the feed value was higher in the leaves and grains, and the leaf and stem had higher feed values than husk or cob.

• Journal of Microbiology and Biotechnology
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• v.11 no.6
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• pp.1093-1098
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• 2001

The gene coding for a Lepidoptera-specific insecticidal crystalline (or control) protein (ICP), recognized as cryIAc, from Bacillus thuringiensis subsp. kurstaki HD-73, was cloned into the vector pBluscript ll SK-, and then transformed in Escherichia coli $DH5{\alpha}$. The clone was named EBtIAc and the chimeric phagemid, as pEBtIAc. Hyperexpression of CryIAc protoxin was observed in the extract of the culture of E. coli harboring pEBtIAc. Crystalline protoxin was purified by differential solubility. It was dissolved in alkaline pH, and exposed to trypsin to be activated. The molecular weights of the pro- and activated toxins on SDS-PAGE were estimated to be ca. 130 kDa and 60 kDa, respectively. The toxicity was tested by force-feeding larvae of gypsi moth (Lymantria diapar) with trypsinized protoxin. Using the batch of biologically active form of the toxin as an immunogen, anti-CryIAc antiserum was raised in a New Zealand white rabbit. Immunoglobulin G was fractionated from the seam by Protein-A sepharose affinity chromatography. Immunoreactivity of the antibody was examined by dot and Westerns blottings. It has been found that the anti- CryIAc antibody recognized the purified toxin at a level below a nanogram in terms of quantity. Using the antibody some of Bt-corns were able to be differentiated from tons of corn kernels which were imported from America as forage crops.

• Journal of The Korean Society of Grassland and Forage Science
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• v.22 no.4
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• pp.253-258
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• 2002

NC+4880, a forage com hybrid, is bred by NC+hybrids Co. in US, and Gast 8396 IT is by Garst Seeds Co. We have tested these two cultivars in Suwon and Chonan over three years to test their regional yield trial. Through above experiments, the excellence is testified, in result, they are determined as new recommended cultivars by NACF. The main characteristics of these cultivars are as tallows ; 1. NC+4880 a. The seed coat is yellow, the mean tasseling date is 7th of July and it takes about 74 days from emergence to tasseling date. This hybrid is a day ahead and the culm length is 242 cm, moreless shorter comparing with a check hybrid, Suwon 19. The ear height 117 cm which is short and its stay green is moderate. b. It shows resistance to H maydis, and MBSDV is barely observed and its stay green is moderate. c. The fresh yield, dry matter yield, and TDN yield per a ha are 55.4 tons, 18.3 tons, 12.4 tons, respectively, which are increased by 6%, 7%, and 9%, respectively, comparing with a check hybrid, Suwon 19. The percent ear among total dry matter yield is 50.9%. 2. Garst 8396 IT a. The seed coat is yellow, the mean tasseling date is 9th of July and it takes about 76 days from emergence to tasseling date. This hybrid represent a day delayed compared with a check hybrid, Suwon 19. The Culm length and the ear height are 275 cm, and 138 cm, respectively. b. It resists moderately to H. maydis, and highly resists to lodging in the field conditions. MBSDV is barely observed and its stay green is moderate. c. The fresh yield per a ha is 64.9 tons, dry matter yield, 20.9 tons, and TDN, 13.8 tons, respectively, which are increased by 25%, 23%, and 22% comparing with a check hybrid, Suwon 19. The percent ear among total dry matters is 43.3%.