• KSBB Journal
• /
• v.25 no.4
• /
• pp.303-310
• /
• 2010

Probiotics beneficially affect the health of the host via various mechanisms in the intestine. Recent developments in probiotic products have mainly been made to maximize probiotic effects in human. In this regard, probiotic products containing doubly coated or encapsulated cells, multi-species probiotics, or high viable cell number (1010 viable cells/gram or more) have been developed and are already available in the market. Until now, the majority of probiotics contain live cells but little attention has been paid to other alternative products such as heat-killed cell or bacteriocin-containing ones, which could have broad applications due to advantages over live cell-based probiotics, such as safety and stability. In addition, genetically engineered lactic acid bacteria could be of great importance in the field of alimentary health if they are carefully designed for biological safety. Although a number of probiotics are marketed by claiming health benefits, regulations for health claims will be more stringent. Therefore sufficient scientific and clinical evidences supporting the safety and efficacy of the potential probiotic strain will be required by the regulatory authority for a health claim, which thus may have a huge impact on the future probiotic market.

• Journal of Microbiology and Biotechnology
• /
• v.28 no.11
• /
• pp.1916-1927
• /
• 2018

Corynebacterium glutamicum is an excellent platform for the production of amino acids, and is widely used in the fermentation industry. Most industrial strains are traditionally obtained by repeated processes of random mutation and selection, but the genotype of these strains is often unclear owing to the absence of genomic information. As such, it is difficult to improve the growth and amino acid production of these strains via metabolic engineering. In this study, we generated a complete genome map of an industrial L-valine-producing strain, C. glutamicum XV. In order to establish the relationship between genotypes and physiological characteristics, a comparative genomic analysis was performed to explore the core genome, structural variations, and gene mutations referring to an industrial L-leucine-producing strain, C. glutamicum CP, and the widely used C. glutamicum ATCC 13032. The results indicate that a 36,349 bp repeat sequence in the CP genome contained an additional copy each of lrp and brnFE genes, which benefited the export of L-leucine. However, in XV, the kgd and panB genes were disrupted by nucleotide insertion, which increase the availability of precursors to synthesize L-valine. Moreover, the specific amino acid substitutions in key enzymes increased their activities. Additionally, a novel strategy is proposed to remodel central carbon metabolism and reduce pyruvate consumption without having a negative impact on cell growth by introducing the CP-derived mutant $H^+$/citrate symporter. These results further our understanding regarding the metabolic networks in these strains and help to elucidate the influence of different genotypes on these processes.

• Journal of Environmental Science International
• /
• v.26 no.2
• /
• pp.231-238
• /
• 2017

Rapid industrial development has led to a serious problem of pollution in the industrial sector. With the increasing social need for environmental protection, research on air pollution prevention equipment for reducing pollutants in industrial processes is actively being undertaken. The deterioration of existent, installed facilities, their increased emission rates, and the strengthening of the effluent quality standards make complying with permissible emission standards difficult. In fact, installing new electric precipitators or complementing existent facilities is inevitable. The expansion and complementation of the installed electrical precipitators have led to improvements in dust collection efficiency, shorter working times, and lower costs. Because of its easy installation and simple manufacturing process, the production method with the discharge electrode of an electric precipitator is widely used. The following conclusions were reached by classifying discharge electrodes into four types based on the production method and mutually comparing them by their dust collection efficiency. None of the four types used in this study were damaged by impact. However, we were able to confirm some strain from the compression sites of both type A and type B. Both type B and type C are expected to have greater dust collection efficiencies than the other models due to their large vibration transmissibility. Moreover, the high vibrational energy is expected to cause rapping damage during its operation. Particularly, in the case of type B, some of the strain was found at the end of the compression site. The coupling schemes of both type C and type D are out of vibration transmissibility. On the other hand, the ability to maintain straightness and solidity of the side is regarded as outstanding and stable. Type D has outstanding on-site workability, considering the presence of locking, structural stability, and work conditions. From these experiments, we determined that type C is the most ideal connection method of discharge electrode, considering its construction period of renovation. Type C is inferior to type D with regard to on-site workability. However, type C has outstanding dedusting transmission with regard to the straightness, solidity maintenance, and vibration of shearing stress.

• Journal of Korean Tunnelling and Underground Space Association
• /
• v.19 no.6
• /
• pp.871-885
• /
• 2017

Nowadays, the construction of tunnels with a shallow depth drastically in urban areas increases. But the effect of sidewall displacement in shallow tunnel on its behavior is not well known yet. Most studies on the shallow tunnel have been limited to the stability and the failure of the tunnel and the adjacent ground in plane strain state. Therefore, the model tests were conducted in a model ground which was built with carbon rods, in order to investigate the impact of the tunnel sidewall displacement on the lateral load transfer to the adjacent ground. The lateral displacement of the tunnel sidewall and the load transfered to the adjacent ground were measured in model tests for various overburdens (0.50D, 0.75D, 1.00D, 1.25D). As results, if the cover depth of tunnel was over a constant depth (0.75D) in a shallow tunnel, the tunnel sidewall was failed with a constant shape not depending on the tunnel cover depth and also not affected by the opposite side of the wall. But, if the cover depth of tunnel was under a constant depth (0.75D), the failure of the tunnel sidewall could affect the opposite sidewall. In addition, if the displacement of tunnel sidewall with 50% of the critical displacement occurred, the tunnel failure was found to be at least 75%. However, additional studies are deemed necessary, since they may differ depending on the ground conditions.

• Journal of Advanced Marine Engineering and Technology
• /
• v.37 no.5
• /
• pp.461-466
• /
• 2013

This paper examines the characteristics of butt welding joint shrinkage for shipbuilding and marine structures main plate. The shrinkage strain of butt welding joint which is caused by the process of heat input and cooling, results in the difference between dimensions of the actual parent metal and the dimensions of design. This, in turn, leads to poor quality in the production of ship blocks and reworking through period of correction brings about impediment on improvement of productivity. Through experiments on butt welding joint's shrinkage strain on large structures main plate, the deformation of welding residual stress in the form of I, Y, V was obtained. In addition, the results of experiments indicate that there is limited range of shrinkage in the range of 1 ~ 2 mm in 11t ~ 21.5t thickness and the effect of heat transfer of weld appears to be limited within 1000mm based on one side of seam line so there was limited impact of weight of parent metal on the shrinkage. Finally, it has been learned that Shrinkage margin needs to be applied differently based on groove phenomenon in the design phase in order to minimize shrinkage.

• Journal of the Korea institute for structural maintenance and inspection
• /
• v.26 no.6
• /
• pp.167-174
• /
• 2022

The objective of this study is to evaluate the dynamic properties of DUS (damping-up system) composed of the materials with excellent damping capacity, and to compare with those of the conventional hangar bolt. The main parameters are the type and hardness (𝜂_H), of rubber and the prestress force (value converted from the compression strain (𝜂_R) in the stress-strain relationship of rubber). The dynamic properties were examined from the natural frequency (𝜔_n), maximum response acceleration (A_m), amplification coefficient (𝛼_p), maximum relative displacement (𝚫_m), and damping ratio (𝜉_D). The test results showed that the A_m, 𝛼_p, and 𝚫_m values of DUS were 46.3%, 46.6% and 62.9% lower, respectively, and the 𝜉_D value was 3.89 times higher, when compared to those of the conventional hangar bolt. In particular, the 𝛼_p value was 1.3 for DUS, and 2.45 for the conventional hanger bolt, which were similar to those of rigid and flexible components specified in KDS 41 17 00, respectively. Consequently, in the optimal details of DUS, the 𝜂_H values of 50 and 45 were required for the NR (natural rubber) and EPDM (ethylene propylene diene monomer), and the 𝜂_R value of 5% was also recommended.

• Animal Bioscience
• /
• v.37 no.4
• /
• pp.689-696
• /
• 2024

Objective: Our study examined the impact of propriety blends of Bacillus strain probiotics on the performance, egg quality, and faecal microflora of laying hens. Methods: A total of 183 Institut de selection Animale (ISA) brown laying hens aged 23 weeks with an average body weight of 1,894±72 g were randomly allocated into 3 groups as control (corn-soybean meal based diet, CON), 0.5 g/kg Enterosure probiotics (ET1, 3×10⁸ colony-forming unit [CFU]/kg feed), and 5 g/kg Enterosure probiotics (ET2, 3×10⁹ CFU/kg feed) administered in mashed form. At the completion of each phase hen day egg production (HDEP), average egg weight (AEW), feed intake, and faecal microbiota were evaluated. Results: HDEP and AEW were higher (p<0.05) in the ET2-supplemented diet in phase 3 (week 9 to 12) compared with CON. Egg mass (EM) was higher (p<0.05) in phase 2 at ET2, and also higher (p<0.05) in phase 3 at the ET1 and ET2-supplemented diets compared with CON. Feed conversion ratio was lower (p<0.05) in phase 3 at the ET1 and ET2-supplemented diets, with ET2 being the lowest compared with ET1 and CON. Yolk colour was higher (p<0.05) in the ET-supplemented diets at phase 3 compared with CON. Bifidobacterium spp. was higher (p<0.05) in the ET2- supplemented diet compared with CON in phase 2, while in In phase 3, Lactobacillus spp. and Bifidobacterium spp. were higher (p<0.05) in the ET-supplemented diets compared with CON. Coliforms were lower (p<0.05) in the ET-supplemented diets compared with CON in phase 3. Conclusion: The propriety blends of Bacillus strain probiotics supplements at 0.5 g/kg and 5 g/kg could improve the production and quality of eggs with more significance at 5 g/kg for HDEP, AEW and EM, which was achieved via the increase of beneficial microbiomes such as Lactobacillus spp., Bifidobacterium spp., and the decrease of pathogenic microbiomes like Escherichia coli and Coliforms which was speculated to improve gut barrier function and the reproductive hormone.

• International Journal of Highway Engineering
• /
• v.13 no.2
• /
• pp.159-166
• /
• 2011

This research was performed to evaluate physical properties of polysulfide epoxy overlay material for bridge deck as part of a review for possibility of domestic application of polymer concrete for bridge deck pavement. In order to evaluate strength characteristics, compressive strength, flexural strength and bond strength were tested, and, for durability characteristics, chloride ion penetration resistance and freeze/thaw resistance were tested along with ultraviolet rays impact evaluation. The tests showed that the results met the criteria suggested by the American Concrete Institute in terms of compressive strength, flexural strength and bond strength. However, in terms of the strengths measured at various test temperatures, it was found that the epoxy material was highly dependent on temperature, and, therefore, this should be considered at the time of domestic application of the epoxy material later. Deflection characteristics was checked through flexural strength test and it was found that bridge deck pavement using the epoxy material was excellent compared to bridge deck pavement using asphalt. Furthermore, the results of chloride ion penetration resistance test and freeze/thaw resistance test were also excellent. In the evaluation of ultraviolet rays impact on epoxy slurry mixture, reduction of strain was noticed with increased strength, but the deflection characteristics after exposure to ultraviolet rays was better than the existing acryl polymer concrete. Therefore, it is concluded from the research that the polysulfide epoxy overlay material has the physical properties that are appropriate to pavement of bridge deck.

• Journal of the Korea institute for structural maintenance and inspection
• /
• v.18 no.1
• /
• pp.119-127
• /
• 2014

The application of sulfur polymer emulsion (SPE) as an acrylate substitute for semi-rigid pavement grout was evaluated, and the performance improvement by employing PVA fibers were also evaluated. The result indicated that the filling ratio of semi-rigid pavement material decreased as the fiber content increased, but it was measured to be 92~94% in every mixing condition, which satisfies the target performance, 90%. The maximum Marshall stability value of semi-rigid pavement material was measured to be 25.4 kN, which is about 4.7 times higher than the Korean Standard required for semi-rigid pavement material, 5.0 kN. The dynamic stability evaluation of semi-rigid pavement material indicated that the resistance to deformation from the wheel tracking test was improved by an SPE substitution, and in every mixing condition, the deformation converged to a constant value after 45 minutes with the same dynamic stability of 31,500 times/mm. The strain at the flexural failure was about 0.53%, which shows superior rigidity to asphalt pavements. The examination of abrasion resistance and impact resistance showed that the loss ratio was 9.8~6.0% in every mixing condition, which indicates a good abrasion resistance. Also, when fiber content ratio was 0.3%, the impact resistance was 2.82 times higher compared to plain (i.e., when fibers were not added). In the limited range of this study, an SPE substitution ratio of 30% was found to be an optimal level considering the mechanical and durability performance. In addition, it is thought that semi-rigid pavement material with superior performance could be manufactured if fiber content ratio up to 0.3% is applied depending on the purpose of use.

• The Journal of the Institute of Internet, Broadcasting and Communication
• /
• v.15 no.3
• /
• pp.201-209
• /
• 2015

The deformed degree of car body varies largely with the collision part from side collision of car-to-car. In case of deformation of car body caused by collision, the movement is different as speed energy changes to strain energy. Generally, in the analysis of traffic accident, the movement of car after the collision is analyzed by law of conservation of motion and the error of energy absorption rate along the deformation of car body can be calibrated by inputting coefficient of restitution, but it is current situation that coefficient of restitution applied by referring to the research results of forward collision and backward collision because the research results of side collision is rare. Vehicle model of finite element method applied by structure of car body and materials of each component was analyzed by explicit finite element method, and coefficient of restitution and collision detection time along contact part of side collision was drawn by analyzing the results. Analysis result acquired through the law of conservation momentum by applying finally-computed coefficient of restitution and crash detection time compared to collision result of actual vehicle. As a result, the reliability of analysis was higher than the existing analysis method were acquired when applying the drawn initial input value that used finite element method analysis model.