• 제목/요약/키워드: Immunomodulation

검색결과 185건 처리시간 0.022초

재생불량성빈혈(再生不良性貧血)의 변증론치(辨證論治)에 대(對)한 고찰(考察) (A Study of Bian Zheng Lun Zhi on Aplastic Anemia)

  • 홍상훈;이승연
    • 대한한방소아과학회지
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    • 제13권2호
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    • pp.79-92
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    • 1999
  • Background/Aims: Aplastic anemia is defined as pancytopenia (anaemia, leucopenia, and thrombocytopenia) result from aplasia of the bone marrow. Many studies have shown that survival rate of aplastic anemia is 50-60% with immunomodulation therapy. In Korea, there is a lack of research considering oriental herbal medicine with aplastic anemia. Methods: It was compared and analyzed that recently several experimental or clinical reports of oriental herbal medicine on aplastic anemia. Results and Conclusion: The oriental herb of Panax ginseng radix, Cprdonopsis pilosula radix, Astragalus membranaceus radix, Atractylodes marcrocephala. Cervi Cornu Parvum, Epimedii Herba, Boshniakiae Herba, Morindae Radix, Angelicae gigantis Radix, Cascutae Semen, Lycii Fructus, Polygoni Multiflori Radix potently stimulated hematopoietic stem cell activity, Response rate to oriental herbal medicine of aplastic anemia was 30-60% and effect rate of aplastic anemia was 73-93%, Bian zheng Lun zhi(辨證論治 treatment according to syndrome differentiation) which based on Shen xu(腎虛) is presumed to approach highest degree effect in response rate.

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Effects of Lycium chinense Miller Fruit and its Constituent Betaine on Immunomodulation in Balb/c Mice

  • Kim, Na-Hyung;Baek, Seung-Hwa
    • 한국환경농학회지
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    • 제33권3호
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    • pp.189-193
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    • 2014
  • BACKGROUND: The aim of this study is to investigate the effects of Lycium chinense Miller fruit and its representative constituent betaine on reduction of immobility time and blood parameters in balb/c mice. METHODS AND RESULTS: We investigated the immobility time and the changes in aspects of blood biochemical parameters by the administration of L. chinense Miller fruit and its representative constituent betaine, after the forced swimming test. The immobility time was significantly reduced about 41.3% and 53.6%, respectively, in the animal of L. chinense Miller fruit and its representative constituent betaine-administrated group for 7 days, in comparison with that of the control group. The level of blood urea nitrogen was significantly decreased in L. chinense Miller fruit and its representative constituent betaine-treated group compared with the control group (P < 0.05), respectively. In addition, the interlukin-2 levels of mice in L. chinense Miller fruit and betaine treated group was increased compared with the control group. CONCLUSION: These results indicate that L. chinense Miller fruit and betaine might be helpful in the immune function improvement, enhance physical stamina, and fatigue recovery.

Immunological Mechanisms by Which Concomitant Helminth Infections Predispose to the Development of Human Tuberculosis

  • Mendez-Samperio, Patricia
    • Parasites, Hosts and Diseases
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    • 제50권4호
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    • pp.281-286
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    • 2012
  • Helminthic infections afflict over 1.5 billion people worldwide, while Mycobacterium tuberculosis infects one third of the world's population, resulting in 2 million deaths per year. Although tuberculosis and helminthic infections coexist in many parts of the world, and it has been demonstrated that the T-helper 2 and T-regulatory cell responses elicited by helminths can affect the ability of the host to control mycobacterial infection, it is still unclear whether helminth infections in fact affect tuberculosis disease. In this review article, current progress in the knowledge about the immunomodulation induced by helminths to diminish the protective immune responses to bacille Calmette-Guerin vaccination is reviewed, and the knowledge about the types of immune responses modulated by helminths and the consequences for tuberculosis are summarized. In addition, recent data supporting the significant reduction of both M. tuberculosis antigen-specific Toll-like receptor (TLR) 2 and TLR9 expression, and pro-inflammatory cytokine responses to TLR2 and TLR9 ligands in individuals with M. tuberculosis and helminth co-infection were discussed. This examination will allow to improve understanding of the immune responses to mycobacterial infection and also be of great relevance in combating human tuberculosis.

표고버섯 약침액(藥鍼液)이 Cytochrome P450 1A1과 1A2 활성 억제에 미치는 효과 (Inhibitory Effect of Lentinus edodes Aqua-acupuncture Solution on the Cytochrome P450 1A1 and 1A2 Activities)

  • 문진영
    • Korean Journal of Acupuncture
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    • 제21권2호
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    • pp.139-145
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    • 2004
  • Objectives : Inhibition of phase I enzymes such as cytochrome P450 (CYP) 1A1 or 1A2 is considered a major mechanism of protection against initiation of carcinogenesis. The inhibition of toxic enzymes and CYP were studied with so many oriental herbral medicine. Recently, numerous polysaccharides and polysaccharide-protein complexes have been isolated from mushrooms and used as a source of therapeutic agents. The most promising biopharmacological activities of these polymers are their immunomodulation and anti cancer. But, in this study the inhibitory effect was on the aqua-acupuncture of Lentinus edodes. Materials : Lentinus edodes aqua-acupuncture solution (LEAS) was prepared and tested for the inhibition of cytochrome P450 (CYP) 1A1 and 1A2 activities. LEAS type I from fruit body of these mushrooms. Type II was extracted from cultured broth of Lentinus edodes mycelum. Results : LEAS type I and type II were significantly inhibited CYP 1A1 and 1A2 enzymes at concentration of 5.0 and 10.0 mg/ml. Conclusion : These results suggested that LEAS may act as block agent against carcinogenesis by inhibition of phase I enzymes.

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발효녹용 추출물에 의한 비장세포의 생존율 및 interleukin-12 생산 증진 (Fermented antler extract enhances the viability and interleukin-12 production of spleen cells)

  • 양혜열;김영수;주홍구
    • 대한수의학회지
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    • 제56권3호
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    • pp.183-187
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    • 2016
  • The effects of antlers have long been known in traditional Asian medicine. However, few studies have investigated the effects of antlers on immunity. In this study, we investigated whether fermented antler extract (FAE) has immunomodulatory effects on spleen cells. FAE enhanced the activity of spleen cells in a concentration dependent manner compared to antler extract. Interestingly, FAE significantly increased the production of interleukin-12, a representative cytokine of cell-mediated immunity, while it marginally increased that of tumor necrosis factor-alpha. Flow cytometry analysis demonstrated that FAE can protect spleen cells from spontaneous cell death without a significant proportional change in subsets, mainly lymphocytes. Taken together, the results of the present study showed that FAE has beneficial effects on spleen cells, a major type of immune cell, indicating that it can function as an immunomodulator without significant cytotoxicity. These data may broaden the use of FAE in basic research and clinical areas.

돌외 에탄올 추출물의 생체방어력 증진효능 (Augmentation of Immune Responses by Oral Administration of Gynostemma pentaphyllum Ethanol Extract)

  • 임선아;최현숙;황방연;이명구;이종길
    • 생약학회지
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    • 제40권1호
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    • pp.35-40
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    • 2009
  • The immunomodulatory activities of the ethanol extract of Gynostemma pentaphyllum, termed hereafter as GPE, were examined in immunosuppressed mice as well as in normal mice in the present study. Oral administration of GPE into mice prevented dexamethasone (DEX)-induced immunosuppression as determined by the mitogen-induced proliferation of the splenocytes and the the cytokine production (TNF-$\alpha$, IL-$1{\beta}$) in the whole blood culture. In addition, oral administration of GPE increased antitumor host defense in mice implanted with sarcoma-180 tumor cells. The immunoaugmenting activity of orally administered GPE was also confirmed in mice immunized with ovalbumin (OVA). Mice that were orally administered with GPE generated much more potent OVA-specific cytotoxic T lymphocyte (CTL) responses upon intravenous OVA injection compared to the untreated controls. These results demonstrate that oral administration of the ethanol extract of Gynostemma pentaphyllum could be useful to increase host defense in immunocompromised situations such as stress- or tumor-induced immunosuppression.

눈꽃동충하초(Paecilomyces japonica DGUM 32001) 균사배양물의 항암 효과에 관한 유세포분석학적 연구 (Flow Cytometrical Investigation on Antitumor Activity of Mycelial Culture of Insect-born Fungus Paecilomyces japonica DGUM 32001)

  • 이지선;이임선;정경수;김용해;한영환;이만형
    • 약학회지
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    • 제45권1호
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    • pp.64-70
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    • 2001
  • Protein-polysaccharide fractions, PJ-3 and PJ-4, were prepared from mycelial culture filtrate of an insect-born fungus, Paecilomyces japonica DGUM 32001, and subjected to a flow cytometrical analysis for their vivo antitumor and immunomodulating activity in ICR mice. When i.p. injected once daily for semen days at 100 mg/kg, PJ-4 exerted a strong antitumor activity showing the growth inhibition ratio of 85.1% against i.p. implanted sarcoma 180 cells, while PJ-3 showed only a weak activity. Moreover, PJ-4 signiscantly increased the expression level of CD25 (IL-2R $\alpha$-chain) as well as forward scatter (FSC) values of splenic CD8$^{8}$ T cells. It is also noteworthy that PJ-4 strongly induced the peritoneal exudate cells in the same experiment. In an in vitro study, PJ-4 slightly inhibited the growth of sarcoma 180 cells at the concentration of 50$\mu$g/ml or higher. These results strongly suggest that PJ-4 might exert its antitumor activity through immunostimulation as well as direct inhibitory activity on the tumor cells.

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Immunomodulating and Anticoagulant Activity of Glycosaminoglycans Derived from Porcine Testis

  • Yoo, Yung-Choon;Kim, Yeong-Shik;Song, Kyung-Sik;Moon, Eun-Ho;Lee, Kyung-Bok
    • Archives of Pharmacal Research
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    • 제25권5호
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    • pp.669-674
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    • 2002
  • Glycosaminoglycans (GAGs) were isolated from the porcine testis, and their immuno-modulating and anticoagulant activity was investigated. From anion exchange chromatography (Dowex Macropolous Resin) used for further isolation of porcine testis GAGs (PT-GAGs), two fractions (PT-GAG-1.5 and PT-GAG-16) eluted by different salt concentration were obtained. In immunomodulating activity test, PT-GAG-1.5, but not PT-GAG-16, significantly enhanced the growth of murine peritoneal macrophages. In addition, treatment with PT-GAG-1.5 induced the production of cytokines, interleukin-1$\beta$ (IL-1$\beta$), interferon-${\gamma}$ (IFN-${\gamma}$) and tumor necrosis factor-$\alpha$ (TNF-$\alpha$), from murine microphages. Unexpectedly, both of PT-GAGs had no effect on the growth of murine splenocytes. The anticoagulant activity of PT-GAG-1.5 and PT-GAG-16 was examined by activated partial thromboplastin time (aPTT) assay and thrombin time (TT) assay. Both of PT-kGAGs significantly increased the clotting times of aPTT and TT in a dose-dependent manner. The anticoagulant activity of PT-GAG-16 was found to be higher than that of PT-GAG-1.5. These results suggest that PT-GAGs possess biological activities such as immunomodulating activity and anticoagulant activity.

침자가 LPS를 주입한 흰쥐 시상하부 방실핵의 신경활성에 미치는 영향 (Effects of Acupuncture on the Neuronal Activation of Paraventricular Nucleus of Hypothalamus in Lipopolysaccharide-injected Rats)

  • 손양선;박히준;김승태;임사비나
    • Korean Journal of Acupuncture
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    • 제19권1호
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    • pp.57-65
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    • 2002
  • 본 연구에서는 침의 면역조절작용을 통한 염증반응 억제효과를 연구하기 위하여 내독소를 주입한 흰쥐의 시상하부에서 염증반응의 중추인 방실핵의 신경활성에 미치는 영향을 관찰하였다. 흰쥐의 미정맥에 LPS와 생리식염수를 각각 주입하고 군에 따라 양측 소부(HT8)나 족삼리(ST36)에 1분간 침치료를 각각 시행하였다. C-Fos는 신경활성을 자극하는 초기단계에 발현되는 유전자로서 신경계의 특정부위의 활성도를 측정하는 지표로 널리 사용되고 있다. 본 연구자는 침자극이 LPS로 인한 염증반응에 미치는 영향과 그 기전을 알아보기 위해 면역조직화학염색의 방법을 이용하여 대뇌 시상하부의 방실핵에서 c-Fos 면역활성을 측정하였다. LPS를 주입한 군의 방실핵에서 생리식염수를 주입한 군에 비해 c-Fos 면역활성이 유의하게 증가한 반면 소부에 자침했을 때 LPS에 의해 증가된 c-Fos 면역활성이 유의하게 감소하였다. 족삼리에 자침한 군에서는 유의한 변화가 나타나지 않았다. 결론적으로 소부 침치료는 LPS로 인해 증가된 방실핵의 신경활성을 효과적으로 감소시켰고 이는 침의 면역조절 및 탁월한 염증억제 효과를 보여주는 결과일 뿐 아니라 침의 인체 항상성 유지를 통한 치료기전에 대한 향후 연구의 중요한 실마리를 제공해주고 있다고 사료된다.

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영지버섯 생장점 단백다당체 GLB-A, GLB-B의 항암효과 및 면역 활성에 관한 유세포 분석학적 연구 (Flow Cytometrical Analysis of the Antitumor and Immunomodulatory Activities of GLB-A and GLB-B, the Protein-polysaccharide Fractions of the Growing Tips of Ganoderma Lucidum)

  • 오정연;정경수
    • 약학회지
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    • 제42권5호
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    • pp.487-493
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    • 1998
  • In the previous study we described the antitumor effect of GLB, a protein-polysaccharide fraction separated from the growing tips of Ganoderma lucidum, against sarcoma 18 0 solid tumor in ICR mice. In this study, we separated an acidic protein-polysaccharide fraction, GLB-A, and a basic protein-polyaccharide fraction, GLB-B, from GLB by differential precipitation, and elucidated their antitumor and immunomodulatory activities. When ip injected at the dose of 50mg/kg/day into the ICR mice, GLB-A and GLB-B inhibited the growth of ip implantated sarcoma 180 cells by 32.4% and 21.0%, respectively. Of these, GLB-A increased the % lymphoblast in the spleen of the tumor-bearing and the normal mice by 20.9% and 123.0%, and the CD4/CD8 ratio by 73.3% and 22.4%, respectively. GLB-A also increased the expression of CD25 (IL-2 receptor alpha ch0ain) in normal mice by 82.0%. These results strongly suggest that GLB-A is a promising candidate for antitumor immunomodulatory medicine.

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