Korean Journal of Acupuncture

  • 제19권1호
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  • Pages.57-65
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  • 2002
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  • 2287-3368(pISSN)
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  • 2287-3376(eISSN)
경락경혈학회 (Society for Meridian and Acupoint)
권호 표지

침자가 LPS를 주입한 흰쥐 시상하부 방실핵의 신경활성에 미치는 영향

Effects of Acupuncture on the Neuronal Activation of Paraventricular Nucleus of Hypothalamus in Lipopolysaccharide-injected Rats

  • 손양선 (경희대학교 한의과대학 경혈학교실) ;
  • 박히준 (경희대학교 한의과대학 경혈학교실) ;
  • 김승태 (경희대학교 한의과대학 경혈학교실) ;
  • 임사비나 (경희대학교 한의과대학 경혈학교실)
  • Son Yang-Sun (Dept. of Meridian & Acupoint, College of Oriental Medicine, Kyung Hee University) ;
  • Park Hi-Joon (Dept. of Meridian & Acupoint, College of Oriental Medicine, Kyung Hee University) ;
  • Kim Seung-Tae (Dept. of Meridian & Acupoint, College of Oriental Medicine, Kyung Hee University) ;
  • Lim Sa-Bi-Na (Dept. of Meridian & Acupoint, College of Oriental Medicine, Kyung Hee University)
  • 발행 : 2002.03.27
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초록

본 연구에서는 침의 면역조절작용을 통한 염증반응 억제효과를 연구하기 위하여 내독소를 주입한 흰쥐의 시상하부에서 염증반응의 중추인 방실핵의 신경활성에 미치는 영향을 관찰하였다. 흰쥐의 미정맥에 LPS와 생리식염수를 각각 주입하고 군에 따라 양측 소부(HT8)나 족삼리(ST36)에 1분간 침치료를 각각 시행하였다. C-Fos는 신경활성을 자극하는 초기단계에 발현되는 유전자로서 신경계의 특정부위의 활성도를 측정하는 지표로 널리 사용되고 있다. 본 연구자는 침자극이 LPS로 인한 염증반응에 미치는 영향과 그 기전을 알아보기 위해 면역조직화학염색의 방법을 이용하여 대뇌 시상하부의 방실핵에서 c-Fos 면역활성을 측정하였다. LPS를 주입한 군의 방실핵에서 생리식염수를 주입한 군에 비해 c-Fos 면역활성이 유의하게 증가한 반면 소부에 자침했을 때 LPS에 의해 증가된 c-Fos 면역활성이 유의하게 감소하였다. 족삼리에 자침한 군에서는 유의한 변화가 나타나지 않았다. 결론적으로 소부 침치료는 LPS로 인해 증가된 방실핵의 신경활성을 효과적으로 감소시켰고 이는 침의 면역조절 및 탁월한 염증억제 효과를 보여주는 결과일 뿐 아니라 침의 인체 항상성 유지를 통한 치료기전에 대한 향후 연구의 중요한 실마리를 제공해주고 있다고 사료된다.

키워드

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