• Title/Summary/Keyword: Immunoglobulin-Y

Search Result 939, Processing Time 0.032 seconds

Production and Isolation of IgY Antibody Raised Against a Lectin Obtained from Maackia fauriei (Maackia fauriei 유래 렉틴에 대한 IgY 항체의 생성 및 분리)

  • Chung Young Yun;Jung Eui Cha;Lee Hyun Jung;Kim HaHyung
    • YAKHAK HOEJI
    • /
    • v.49 no.1
    • /
    • pp.6-10
    • /
    • 2005

  • Immunoglobulin Y (IgY) obtained from chicken as the immunization host brings several advantages to antibody production, such as improved yield, lower cost, longer stability and higher specificity than mammalian immunoglobulin. In the present study, we attempted to produce IgY against a sialic acid-binding lectin, Maackia fauriei agglutinin (MFA), from egg yolk of white Leghorn hens. For the isolation of IgY from egg yolk, we applied a water dilution method. The weekly yield of IgY was determined by enzyme-linked immunosorbent assay, with a final yield of anti-MFA IgY from total IgY of approximately $1\%$. The yielded IgY were used to prepare IgY-affinity column conjugated with CNBr-activated Sepharose 4B, which resulted in the lectin being successfully purified in a single step from Maackia fauriei. This purified lectin exhibited the same hemagglutination activity as lectin purified using conventional purification methods.

  • PDF KSCI

Inhibitory Effects of Chicken Egg Yolk Antibody on Infection of Escherichia coli in Macrophage

  • Lee, Jin-Ju;Kim, Dong-Hyeok;Lim, Jeong-Ju;Kim, Dae-Geun;Kim, Gon-Sup;Min, Won-Gi;Lee, Hu-Jang;Rhee, Man-Hee;Chang, Hong-Hee;Kim, Suk
    • Journal of agriculture & life science
    • /
    • v.46 no.2
    • /
    • pp.107-114
    • /
    • 2012

  • The present study evaluated the potential use of immunoglobulin prepared from egg yolk of chickens immunized with Escherichia coli K88 (IgY-Ec) in the control of E. coli K88 infection in RAW 264.7 murine macrophage. The binding activity of IgY-Ec against E. coli K88 surface protein was more specific and increased than control IgY. In infection assay of E. coli in macrophage, the specific IgY-Ec to E. coli K88 remarkably inhibited the phagocytic activity comparing to nonspecific IgY (p<0.001). In adherence assay, bacterial adhesion on macrophage cells was definitely reduced by preincubation of IgY-Ec compared with nonspecific IgY (p<0.05). These findings suggested that IgY-Ec have the protective effects against pathogens and IgY-based diets may have potential benefits for preventing or treating various infections in domestic animals.

  • KSCI