• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2000년도 춘계학술발표대회
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• pp.378-381
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• 2000

The optimal conditions and properties for the immobilization of marine bacterium Pseudomonas aeruginosa BYK-2 have been determined. For the high productioon of biosurfactant, Na-alginate, PVA, modified PVA were used as a carrier. The optimal emulsifying activity on immobilized Pseudomonas aeruginosa BYK-2 showed 1036Unit (about 2.2g/L biosurfactant) in Basal salt medium(B.S.M.) at $25^{\circ}C$, 100rpm. Ca-alginate was selected the optimal bead among PVA, modified PVA and Ca-alginate. The optimal cell load in alginate bead was 10 gCWW/100g carrier. As the results of incubation of immobilized 5g Ca-alginate bead (conditions; 3% alginate, bead diameter: 2.3mm, 10% cell load) in 50m1 production medium, The emulsifying activity of 1407Unit, about 3.0g/L biosurfactant was obtained from immobilized cell after cultivation of 92hr at $25^{\circ}C$, 100rpm.

• 한국미생물생명공학회:학술대회논문집
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• 한국미생물생명공학회 1978년도 추계학술대회
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• pp.207.2-207
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• 1978

Arthrobacter simplex (ATCC 6946) was cultured, induced and immobilized in acrylamide polymer. The characteristics of the immobilized whole-cell enayme were studied using hydrocortisone as the substrate. The enzyme activity was increased during the incubation of the gel particle in 0.5％ peptone media. The ennzyme reaction kinetics of the Δ'-dehydrogenase (3-oxosteroid Δ'-oxydo reductase, E. C. 1.3.99.4) foliowed the Michaelis-Menten type. Km and Vm values were different significantly after immobilization of the cell. The optimum pH and temperature were changed, too. Nitrogen sources such as casitone, peptone or tryptone were good media for the enzyme reaction. And there was no need to add cofactors of the enzyme in the pre-sence of energy sources used in the test. The effect of metal ions on the enzyme activity was insignificant. Organic solvents were used increase the substrate concentration and there was no optimum solvent concentration depending on the substrate concentration.

• Biotechnology and Bioprocess Engineering:BBE
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• 제9권5호
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• pp.352-355
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• 2004

Heliothis zea (HzAM1) insect cells were immobilized in microspheres by sodium-cellulosesulfate (NaCS) and polydiallyldimethylammoniumchloride (PDADMAC). The highest HzAMl cell density was $7.5{\times}10^7$ cells/mL in the microspheres. After infection of the immobilized cells by Heliothis zea single nuclear polyhedrosis virus (HzSNPV), the highest concentration of HzSNPV (polyhedral inclusion bodies: PIBs) produced was $2.87{\times}10^{10}$ PIBs/mL in the microspheres.

• KSBB Journal
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• 제5권3호
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• pp.223-227
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• 1990

Ethanol을 주로 생산하는 균주인 Z. mobilis의 cell wall 투과성을 높인 후 고정화하여 sorbitol 생산에 이용 하였다. 그러나, toluene으로 투과성을 높은 cell은 oxidoreductase의 유출, 손실로 인하여 sorbitol conversion efficency가 급격히 저하되었다. 따라서, 이와같은 enzymcd의 유출을 방지 하기 위하여, 투과성을 향상시킨 cell을 0.25% glutaraldehyd로 처리한 후 alginate와 chitin에 고정화하여 회석율 $0.2h^{-1}$에서 연속배양을 한 결과 210시간동안 효소활성도의 저하는 거의 일어나지 않았다. 이와같은 연속배양에서 얻어진 sorbitol productivity는 3.5g / l-h로 측정 되었다.

• Journal of Microbiology and Biotechnology
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• 제14권2호
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• pp.395-400
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• 2004

Choristoneura fumiferana (Cf-2Cl) insect cells were cultured and immobilized by using cellulosesulfate (NaCS) and polydiallyldimethylammoniumchloride (PDADMAC). A concentration of CfMNPV (Choristoneura fumiferana multiple-mucleopolyhedrovirus) and a Cf-2Cl cell density in the microspheres have been achieved at the densities of $1.57\times10^{10}$ PIBs/ml and $7.5\times10^7$ cells/ml, respectively. Additionally, MTT-test was used to measure the viable cell density in the microspheres, and the confidence of MIT-test was investigated before and after baculovirus infection in the immobilized cell culture.

• 한국환경과학회지
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• 제6권1호
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• pp.61-67
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• 1997

미생물에 의한 흡착법으로 중금속을 효과적으로 제거, 회수할 수 있다. 알콜 발효 후 부산물로 생성되는 폐 Saccharomyces cerevisiae는 비교적 가격이 저렴하고, 중금속 생체흡착에 유용한 자원으로 이용될 수 있다. 생체흡착 실험에 사용된 미생물은 부유 및 alginate에 고정화된 S. cerevisiae로 수행하였다. 회분식 실험에서 생체흡착량은 Pb > Cu > Cd의 순으로 이루어졌다. Pb 이온의 흡착 평형은 Freundlich와 Langinuir 모델로 설명하였고, Freundlich 모델이 실험자 료와 잘 부합되었다 고정화된 S. cerevisiae를 이용한 혼합용액( Pb, Cu, Cr 및 Cd )흡착 실험에서 각 중금속들은 선택적 흡착 특성을 나타내었다. 고정화 미생물을 고정층 반응기에 충진하여 혼합 중금속 용액의 생체흡착 실험을 수행한 결과 Pb 이온이 가장 많이 흡착을 하였다. 고정화된 미생물에 흡착된 Pb의 탈착실험에서 0.1M의 HCI 및 $H_2SO_4$가 효과적이었다.

• KSBB Journal
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• 제21권5호
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• pp.384-388
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• 2006

본 연구에서는 고정화 방선균을 이용한 이차대사산물생산 공정 개발에 있어서 고려해야 할 중요한 사항인, 고정화세포의 반복사용 가능성을 조사하였다. 또한 축축한 담체에 균사체 형태의 생산균주의 고정화가능성에 대하여 알아보았다. 셀라이트 담체에 고정화된 고생산성 Streptomyces lincolnensis 돌연변이주를 이용한 반복회분식 배양에서, 회분수가 증가함에 따라 고정화세포의 린코마이신 생산성이 증가하였고 9번째 회분에서는 1007 $({\pm}256)$ mg/L의 린코마이신이 얻어졌다. 10번의 반복회분동안 고정화세포에 의해 얻어진 린코마이신 총양을 기준으로 계산된 고정화세포 배양의 생산성은 현탁회분식 배양에 비해 1.4배 높았다. 축축한 담체와 배지가 포함된 플라스크에 균사체 형태의 생산균주를 접종하고 배양 후 생성된 고정화세포 농도와 린코마이신 양은 건조한 담체에 포자상태의 생산균주를 고정화한 후 배양하여 얻어진 것에 비하여 다소 높음이 관찰되었다. 이는 균사체 형태의 생산균주가 축축한 담체에 쉽게 고정화되는 것을 의미한다. 실제 생산규모를 고려할 때, 본 연구팀에서 개발한 방선균 고정화방법은 담체 멸균 및 세포고정화 단계에서 추가적인 설비가 필요 없으며, 이는 고정화단계에서 숙련된 기술과 추가의 설비를 요하는 다른 방법들에 비하여 실용적이며 경쟁력 있는 생물 공정이라고 사료된다.

• 한국미생물·생명공학회지
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• 제22권3호
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• pp.296-303
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• 1994

An immobilized Trigonopsis variabilis cells having an high activity of D-amino acid oxidase(DAO) was used to convert CPC into GL-7-ACA. The optimal pH of the reaction system was 8.0-8.5, and the optimal temperature was 40$\circ$C. When immobilized cell was used repeatedly in semi-batchwise reaction, the system retained 80% of the initial activity after used of 12 times for over 12 hours. The storage stability of the immobilized cell was maintained for 30 days at 4$\circ$C. The CPC concentration for the maximal reaction rate was about 30 mM and 40 mM for free and immobilized cells, respectively. Substrate inhibition of CPC concentration more than 50 mM was overcomed by 20~25% by immobilization. Pure oxygen supply into reaction system was most efficient in D-amino acid oxidase reaction. Continuous conversion to GL-7-ACA from CPC has been developed with an bioreactor system containing immobilized T variabilis cells. By opera- tion of the reactor for 5 hours, the average conversion yield of >80% and GL-7-ACA production of 40~45 mM per hour could be obtained.

• 한국미생물·생명공학회지
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• 제24권6호
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• pp.705-711
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• 1996

For the efficient production of 2, 5-diketo-gluconic acid (2, 5-DKG) by the immobilized cells of Erwinia herbicola, basic characteristics of 2, 5-DKG fermentation were analyzed and a process employing immobilized cell reactor was developed. The immobilized cells appeared to have diffusion limitation, and a maximum production of 2, 5-DKG was accomplished with 2 mm diameters of immobilized beads. Long-term stabilities of the immobilized cells could be maintained by addition of 1.75% (w/v) polypep- tone. Repeated batch fermentations with about 80 mol% of 2, 5-DKG yields were carried out six times in the fluidized bubble column reactors filled with immobilized cells at an aeration rate of 6 vvm.

• Journal of Microbiology and Biotechnology
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• 제7권6호
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• pp.438-440
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• 1997

A continuous fermentation system employing immobilized cells of Zymomonas mobilis and Saccharomyces cerevisiae was studied for the mass production of ethanol. Ethanol production by cells immobilized with Ca-alginate was better than those by cells immobilized with K-carrageenan. Maximum ethanol production employing a continuous system by cells immobilized with Ca-alginate was 77.5 $g.l^{-1}h^{-1}$ at a dilution rate of 1.85 $h^{-1}$ with 82% conversion rate for Z. mobilis while that was 40.2 $g.l^{-1}h^{-1}$ at a dilution rate of 0.92 $h^{-1}$ with 85% conversion rate for S. cerevisiae. These results suggest that Ca-alginate is a better cell immobilization matrix than K-carrageenan and that immobilized cells of Z. mobilis are more efficient than S. cerevisiae for ethanol production.