Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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고정화 균체를 이용한 2,5-Diketo-Gluconic Acid 발효생산

  • 신봉수 (연세대학교 공과대학 식품생물공학과 및 생물산업소재연구센터) ;
  • 신철수 (연세대학교 공과대학 식품생물공학과 및 생물산업소재연구센터)
  • Published : 1996.12.01
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Abstract

For the efficient production of 2, 5-diketo-gluconic acid (2, 5-DKG) by the immobilized cells of Erwinia herbicola, basic characteristics of 2, 5-DKG fermentation were analyzed and a process employing immobilized cell reactor was developed. The immobilized cells appeared to have diffusion limitation, and a maximum production of 2, 5-DKG was accomplished with 2 mm diameters of immobilized beads. Long-term stabilities of the immobilized cells could be maintained by addition of 1.75% (w/v) polypep- tone. Repeated batch fermentations with about 80 mol% of 2, 5-DKG yields were carried out six times in the fluidized bubble column reactors filled with immobilized cells at an aeration rate of 6 vvm.

Keywords

References

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