• KOREAN JOURNAL OF CROP SCIENCE
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• v.42 no.4
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• pp.384-391
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• 1997

In the cultivation of Bupleurum falcatum, one of the problems to surmount is long-term germination period and unstable germination. This experiment was done to examine the effect of $GA_3$ concentration [0(water), 0.01, 0.lmM], chilling, their treatment period [2, 4, 8 days ($GA_3$) : 0, 2, 4 weeks (chilling)] and light quality (red, white, dark) given during the period as pretreatment before sowing on the seed germination of its two cultivars (cv. Jaerae, cv. Jangsu). Light treatment was given during all the periods of $GA_3$ treatment or for 0, 2, 4 days at the end of the chilling treatment. There was no difference in the mean germination rate between the levels of all the treatments except the $GA_3$ concentration meaning that water imbibition and $GA_3$ treatment had the same effect. As light quality treatment during the water imbibition was forced, the mean germination rate of Jaerae, 2 to 4 days imbibition period or red light was more increased or accelerated compared to the other levels of the same treatment, respectively. The rate of Jaerae not affected by the light quality was the greatest in the 2 days water imbibition while the rate of Jangsu was the greatest when water-imbibed for 4 days or treated by red light. No chilling before sowing showed the highest rate due to the light quality and white light forced after sowing had greater rate than the dark treatment. Although there was no difference between the rates of light quality treatment levels in the condition of no chilling before sowing and white light treatment after sowing, the rate of Jangsu was enhanced or accelerated only under illumination during 2 days water imbibition before sowing.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.47 no.6
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• pp.427-431
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• 2002

Lateral roots of soybean sprout might reduce the quality. The study was done to measure the effect of light quality treated during 24 hour imbibition or 6 day culture on growth and development of soybean sprouts on the 6th day after culture. With the soybean seeds imbibed in 4 ppm benzyladenopurine (BA) solution for last 6 hours of the imbibition, blue and red lights were treated during the imbibition, but during 6 day culture, blue and red or far-red light treatments were done for 50 minutes or 5 hours a day, respectively, the periods taking for their cotyledons to turn green color, On the 6th day after culture, the soybean sprouts were classified by 4 categories on the base of hypocotyl length;＞7cm, 4 to 7cm, ＜4cm and non-germination, and their lateral roots, hypocotyl diameters and fraction dry weights were measured. Blue and red lights treated during the imbibition completely blocked lateral root formation regardless of the lights treated during the culture, and showed nearly the same rate of hypocotyls of longer than 4cm. The period of each light treatment forced during the culture did not influence the growth of soybean sprouts. far-red light treated for 5 hours everyday, however, had the least rate of seed germination and hypocotyls of longer than 7cm of the light quality treatments. In addition, red and far-red lights almost equally having the commercial soybean sprouts of longer than 4cm hypocotyls move elongated and selenderized than blue light and dark treatment, meaning the growth and morphology of soybean sprouts was affected by light treatments during the culture.

• Korean Journal of Medicinal Crop Science
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• v.9 no.1
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• pp.68-75
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• 2001

Reasonable seedling emergence is a prerequisite for successful crop cultivation especially in medicinal crops. The study was carried out to model the pretreatment or seed treatment of Codonopsis lanceolata seeds by evaluating the treatment effects of priming, $GA_3$, drying and water imbibition after drying on their germination and then their successive seed treatment on the basis of its seedling emergence. The priming using $Ca(NO_3)_2$ and $GA_3$ treatment under their different concentrations and light quality illuminated for 12 hours a day were separately done and the two best results from priming and $GA_3$ treatments were compared to determine the better one. The drying of imbibed seeds using the above best result and water imbibition of the dried seeds were successively done to measure the rates of germination and emergence. On the greatest germination rate of the first two individual treatments, priming was obtained at $Ca(NO_3)_2$ 150 mM under blue, red light or darkness for 2 days but $GA_3$ was done at 0.1 mM under blue light imposed for 3 days. However, the result of the latter $GA_3$ treatment was better than that of priming. $GA_3$ treated seeds were best desiccated under $35^{\circ}C$ and 4 hour red light illumination. A day water imbibition immediately before sowing increased the germination rate of seeds dried after $GA_3$ treatment. Seedling emergence tested after all 3 successive treatments, $GA_3$, drying and water imbibition before sowing was greater than the two others, only $GA_3$ treatment and the combination of $GA_3$ and drying, meaning that its pretreatment of seeds or seed treatment must follow the successive procedure of the above 3 ones.

• Journal of Life Science
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• v.25 no.2
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• pp.158-163
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• 2015

Rice embryo is more abundant than endosperms in nutrients such as proteins, lipids, and vitamin B1. In this study, we constructed p53 plasmid that could be expressed in a plant system, and investigated optimal germination conditions in a variety of media. For construction of p53 plasmid, we performed p53 amplification from pCDNA-p53, subcloned to TA cloning vector, and then reconstructed into pGEM-CaMV plant expression vector. On the other hand, we prepared a variety of imbibition buffers and complete media for efficient germination of the rice embryo. Imbibition buffers prepared with different concentrations of salt or detergent showed no significant effect on germination efficiency. We prepared further culture media, such as solid agar, liquid media, and paper towel to establish the optimal conditions. Rice embryo showed germination rates of more than 70% in the solid medium, more than 60% in the paper towel medium, but less than 25% in liquid media, although germination rate did not differ with varying concentrations of salt and sucrose in culture media. Under the optimal germination conditions, we introduced the p53 plasmid using imbibition method, and finally detected human p53 gene expression in the germinated rice embryo. This method might present a novel, practical approach for evaluating efficient gene expression utilizing imbibition method in rice embryo.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.58 no.4
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• pp.416-423
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• 2013

Germination is controlled by internal factors of seeds and external factors such as water, temperature and light. We investigated the relationship between germination characteristics of four waxy rice cultivars and patterns of water uptake, antioxidant enzymes and free soluble sugars during early imbibition. Seed viabilities by tetrazolium test of four different rice cultivars were higher than 95% and germination rates of the hulled rice seeds were on 95% average. However, germination rate of intact rice among four cultivars showed a big difference depending on temperature. Water uptake of hulled and intact rice seeds during imbibition reached a stationary phase at around 30% moisture content. Although rates of water uptake were faster in hulled rice and high temperature than intact rice and low temperature condition, difference of those among cultivars was greater under low temperature than high temperature. The time required for rice seeds to uptake 30% water was negatively correlated with percentage of germination, germination energy, germination speed and mean germination time. Guaiacol peroxidase activity at 24h of imbibition was correlated with germination energy and germination speed but not percentage of germination. Catalase activity, soluble protein and maltose concentration at 24h of imbibition were not correlated with characteristics of germination. These results suggest that a time required for rice seeds to uptake 30% of water significantly correlated with germination and guaiacol peroxidase activity during early imbibition plays an important role in initiation of germination.

• Journal of Microbiology and Biotechnology
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• v.10 no.3
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• pp.415-418
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• 2000

Productivity of a rice plant is greatly influenced by salt stress. One of the ways to achieve tolerance to salinity is to transfer genes encoding protective enzymes from other organisms, such as microorganisms. The bacterial gene, mtlD, which encodes mannitol-1-phosphate dehydrogenase (Mtl-DH), was introduced to the cytosol of a rice plant by an imbibition technique to overproduce mannitol. The germination and survival rate of the imbibed rice seeds were markedly increased by transferring the mtlD gene when it was delivered in either a pBIN19 or pBmin binary vector. When a polymerase chain reaction was performed with the genomic DNAs of the imbibed rice leaves as a template and with mtlD-specific primers, several lines were shown to contain an exogenous mtlD DNA. However, a reverse transcription (RT)-PCR analysis revealed that not all of them showed an expression of this foreign gene. This paper demonstrates that the growth and germination of rice plants transiently transformed with the bacterial gene, mtlD, are enhanced and these enhancements may have resulted from the experssion of the mtlD gene. The imbibition method empolyed in this study fulfills the requirements for testing the function of such a putative gene in vivo prior to the production of a stable transgenic plant.

• Korean Journal of Plant Resources
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• v.11 no.2
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• pp.124-130
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• 1998

It failed occasionally to take a reasonable emergence rate since tobacco (Nicotiana tabacum L.) seeds were planted on late Jan. showing lowest temperature. This experiment was done to measure the effect of GA3 (concentration ; period) , light quality (red ; white ; dark) during or after its treatment, daily irradiation hours( 0 ; 8 ; 12 ;16) and germination termperature (20 or 10 $^{\circ}C$ ocnstant ; 20/1$0^{\circ}C$ alternating) on the germination rate. Red and white light given during grmination showed no differences between the other daily irradiation hours except that 8 hours red light delayed germination although their 12 hours irradiation had the gratest rate. The rate was increased with increased concentration to GA3 0.01 mM or increased imbibition period to 3 days although the rate of cv. NC 82 was less than that of cv. Burley 21 in the case of dark imbibition of GA3 but daily 12 hours irradiation during germination. Light quality forced during GA3 imbibition eliminated such effect of GA3 shown in the darkness so that only light quality pretreatment and termperature during germinition were affected on the rate. The germination rate of thecultivars was decreased in the order of red, white light, darkness meaning that it was highly influenced by the light quality during GA3 treatment. Regardless of GA3 or light quality treatment,on the other hand, the rate was greater in 20 $^{\circ}C$ constat than 1$0^{\circ}C$ constant and 20/1$0^{\circ}C$ alternating germination temperature having similar germinative patterns.

• Korean Journal of Medicinal Crop Science
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• v.8 no.4
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• pp.319-326
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• 2000

This experiment was carried out to determine the effects of $GA_3$ treatment (concentration: period) to rhizomes before planting on their growth, morphology and yield. $GA_3$ treatment increased the number of shoots, leaves or latent buds, plant height, and leaf length per plant compared with non-treatment. The best treatment concentrations were 50 ppm in stem per plant, 100 ppm in latent bud and rhizome fresh weight per plant and 250 ppm in shoot fresh weight per plant. The number of leaves and roots per plant, plant height and fresh weights of shoot and root were the greatest in one day imbibition of $GA_3$. Numbers of stems and latent buds per plant and rhizome fresh weight, however, were the highest in two day imbibition. In addition, the most rhizomes per land unit were produced in one day imbibition treatment of 100 ppm $GA_3$.

• Korean Journal of Medicinal Crop Science
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• v.9 no.2
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• pp.99-107
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• 2001

Germination of Codonopsis pilosula seeds and afterward their seedling emergence have been occasionally very poor due to their dormancy and structural defects. The study was done to determine the treatment effects of priming, $GA_3$, agitation and washing during and after priming, drying and water imbibition after drying on germination of its seeds and then of the successive three combined treatment after priming on the its seedling emergence to model its presown seed treatment. While agitation at the speed of 10 rpm was made or not, priming using $Ca(NO_3)_2$ and $GA_3$ under their different concentrations and light quality illuminated for 12 hours a day were done separately. Then their two best results were compared to determine the better one. Using the above determined best result, washing was done 3 minutes in the tap water or not, and drying of washed seeds was made under 4 different lights, and water imbibition of the dried seeds with differed period were successively done to deduce its presown seed treatment. In the comparison of the best germination rate of priming and $GA_3$, the former showed considerably higher rate than the latter; the priming treatment was done at agitated $Ca(NO_3)_2$ 150 mM solution under darkness or 12 hour red light illumination a day for 2 days. Washing after priming enhanced the rate compared to no-washing. Drying was the best way at which seeds washed were desiccated under $35^{\circ}C$ and 4 hour red light illumination. Water imbibition immediately before sowing must be made for 2 days. Seedling emergence rate was the highest at the seeds from successive 4 treatments, priming, washing, drying and water imbibition before sowing of two others, the priming and washing, or the priming, washing and water imbibition, meaning that its presown seed treatment must follow the procedure of the above 4 successive ones.

• Korean Journal of Plant Resources
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• v.12 no.2
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• pp.95-101
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• 1999

Higher and uniform germination should be necessary because many commercial pepper (Capsicum annum L.) seedlings were nowadays sold to farmer. The experiment was carried out to determine the effect of its cultivars (Daemyung; Wanggochu), GA$_3$ (concentration; period), light quality (red; far-red; blue; dark) during GA$_3$imbibition, and germination temperature (GT: 25 or 15$^{\circ}C$ constant; 25/15$^{\circ}C$ alternating) on the rate of germination done under incandescent lamps until 9 days after sowing. Final seed germination was not different between GA$_3$0 to 1.0 mM concentrations but the elapsed days to 50% germination $(T_{50})$ were more reduced by GA$_3$ treatment than water imbibition. Under $25^{\circ}C$ constant germination temperature, earlier germination was enhanced by GA$_3$treatment showing the lowest rate at darkness, although the final germination rates of water imbibition and GA$_3$ treatments were same. The final germination rates of alternating and 25 $^{\circ}C$ constant GT in cv. Daemyng was also equal, while the germination rates of $25^{\circ}C$ and 15$^{\circ}C$ constant GT were the highest and the lowest regardless of cultivars. There was no difference between light quality treatments impelled during GA$_3$ imbibition when light treated seeds were germinated at alternating and $25^{\circ}C$ constant GT. At 15$^{\circ}C$ constant GT, however, red light or dark treatment during GA$_3$imbibition increased the germination rate since 5 days after sowing.