• 제목/요약/키워드: Imbalance Response

검색결과 106건 처리시간 0.029초

Analysis of Protease and Antiprotease Concentrations in Retired Workers Exposed to Inorganic Dusts

  • Shin, Jae-Hoon;Hwang, Joo-Hwan;Lee, Kyung-Myung;Lee, Jong-Seong;Lee, Jeong-Oh;Choi, Byung-Soon;Kim, In-Sik
    • 대한의생명과학회지
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    • 제15권4호
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    • pp.309-317
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    • 2009
  • Occupational exposure to inorganic dusts such as coal and silica has been identified as a chronic obstructive pulmonary disease (COPD) risk factor. This risk factor causes lung inflammation and protease-antiprotease imbalance. This abnormal inflammatory response of the lung induces parenchymal tissue destruction and leads to progressive airflow limitation that is characteristics of COPD. The aim of this study was to determine the relationship of proteases such as neutrophil elastase (NE) and matrix metalloproteinase (MMP)-9 and antiproteases such as alpha-1 antitrypsin (AAT) and tissue inhibitors of metalloproteinase (TIMP)-1 with lung function. The study population contained 223 retired workers exposed to inorganic dusts. We performed lung function test, including percent of forced expiratory volume in one second ($%FEV_1$) predicted and $%FEV_1$/forced vital capacity (FVC). We analyzed serum MMP-9, AAT, TIMP-1 and plasma NE concentrations by sandwich enzyme immunoassay. NE, AAT, and TIMP-1 concentrations in workers, who had $%FEV_1$<80% predicted, were higher than those of workers who had $%FEV_1{\geq}80%$ (P<0.05). Both AAT and TIMP-1 concentrations in workers with airflow limitation were higher than those of workers with normal airflow (P<0.05). $%FEV_1$ predicted showed significant negative correlation with AAT (r=-0.255, P<0.0l), TIMP-1 (r=-0.232, P<0.01), and NE (r=-0.196, P<0.01). $%FEV_1$/FVC predicted showed significant negative correlation with NE (r=-0.172, P<0.05). From the results of stepwise multiple regression analysis about $%FEV_1$ and $%FEV_1$/FVC, significant independents were NE (r=-0.135, P=0.001) and AAT (r=-0.100, P=0.013) in $%FEV_1$, and NE (r=-0.160, P=0.014) in $%FEV_1$/FVC. In the present study, there were significant correlations between airflow limitation and protease concentration and between airflow limitation and antiprotease concentration. Serum protease and antiprotease concentrations, however, may be affected by the biological and inflammatory responses. It is necessary to evaluate specimens more reflected the effects of proteases and antiproteases in the lung such as lung tissue, bronchoalveolar lavage fluid, and exhaled breath condensate (EBC).

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Elevated Serum IL-17A but not IL-6 in Glioma Versus Meningioma and Schwannoma

  • Doroudchi, Mehrnoosh;Pishe, Zahra Ghanaat;Malekzadeh, Mahyar;Golmoghaddam, Hossein;Taghipour, Mousa;Ghaderi, Abbas
    • Asian Pacific Journal of Cancer Prevention
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    • 제14권9호
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    • pp.5225-5230
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    • 2013
  • Background: There is a Th1/Th2 cytokine imbalance and expression of IL-17 in patients with brain tumours. We aimed to compare the levels of IL-17A and IL-6 in sera of glioma, meningioma and schwannoma patients as well as in healthy individuals. Materials and Methods: IL-17A and IL-6 levels were measured in sera of 38 glioma, 24 meningioma and 18 schwannoma patients for comparison with 26 healthy controls by commercial ELISA assays. Results: We observed an increase in the IL-17A in 30% of glioma patients while only 4% and 5.5% of meningioma and schwannoma patients and none of the healthy controls showed elevated IL-17A in their sera ($0.29{\pm}0.54$, $0.03{\pm}0.15$ and $0.16{\pm}0.68$ vs. $0.00{\pm}0.00pg/ml$; p=0.01, p=0.01 and p=0.001, respectively). There was also a significant decrease in the level of IL-6 in glioma patients compared to healthy controls ($2.34{\pm}4.35$ vs. $4.67{\pm}4.32pg/ml$; p=0.01). There was a direct correlation between the level of IL-17A and age in glioma patients (p=0.005). Glioma patients over 30 years of age had higher IL-17A and lower IL-6 in their sera compared to the young patients. In addition, a non-significant grade-specific inverse trend between IL-17A and IL-6 was observed in glioma patients, where high-grade gliomas had higher IL-17A and lower IL-6. Conclusions: Our data suggest a Th17 mediated inflammatory response in the pathogenesis of glioma. Moreover, tuning of IL-6 and IL-17A inflammatory cytokines occurs during progression of glioma. IL-17A may be a potential biomarker and/or immunotherapeutic target in glioma cases.

Expression of Heat Shock Protein and Antioxidant Genes in Rice Leaf Under Heat Stress

  • Lee, Dong-Gi;Ahsan, Nagib;Kim, Yong-Goo;Kim, Kyung-Hee;Lee, Sang-Hoon;Lee, Ki-Won;Rahman, Md. Atikur;Lee, Byung-Hyun
    • 한국초지조사료학회지
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    • 제33권3호
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    • pp.159-166
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    • 2013
  • We have previously investigated the proteome changes of rice leaves under heat stress (Lee et al. in Proteomics 2007a, 7:3369-3383), wherein a group of antioxidant proteins and heat shock proteins (HSPs) were found to be regulated differently. The present study focuses on the biochemical changes and gene expression profiles of heat shock protein and antioxidant genes in rice leaves in response to heat stress ($42^{\circ}C$) during a wide range of exposure times. The results show that hydrogen peroxide and proline contents increased significantly, suggesting an oxidative burst and osmotic imbalance under heat stress. The mRNA levels of chaperone 60, HSP70, HSP100, chloroplastic HSP26, and mitochondrial small HSP responded rapidly and showed maximum expression after 0.5 or 2 h under heat stress. Transcript levels of ascorbate peroxidase (APX), dehydroascorbate reductase (DHAR) and Cu-Zn superoxide dismutase (Cu-Zn SOD) showed a rapid and marked accumulation upon heat stress. While prolonged exposure to heat stress resulted in increased transcript levels of monodehydroascorbate reductase, peroxidase, glyoxalase 1, glutathione reductase, thioredoxin peroxidase, 2-Cysteine peroxiredoxin, and nucleoside diphosphate kinase 1, while the transcription of catalase was suppressed. Consistent with their changes in gene expression, the enzyme activities of APX and DHAR also increased significantly following exposure to heat stress. These results suggest that oxidative stress is usually caused by heat stress, and plants apply complex HSP- and antioxidant-mediated defense mechanisms to cope with heat stress.

LncRNA CRNDE Promotes the Progression of B-cell Precursor Acute Lymphoblastic Leukemia by Targeting the miR-345-5p/CREB Axis

  • Wang, Weimin;Wu, Feifei;Ma, Ping;Gan, Silin;Li, Xue;Chen, Li;Sun, Ling;Sun, Hui;Jiang, Zhongxing;Guo, Feng
    • Molecules and Cells
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    • 제43권8호
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    • pp.718-727
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    • 2020
  • The imbalance between the proliferation and apoptosis of B-cell precursors is an important contributor to the pathogenesis of B-cell precursor acute lymphoblastic leukemia (BCP-ALL), while its specific regulatory mechanism remains perplexing. This study aimed to expound the underlying mechanism of the proliferation and apoptosis of BCP-ALL cells from the perspective of non-coding RNA. In this study, long non-coding RNA colorectal neoplasia differentially expressed (LncRNA CRNDE) was upregulated in the bone marrow of BCP-ALL patients and BCP-ALL cell lines (NALM-6 and RS4;11). Functionally, LncRNA CRNDE knockdown restrained cell proliferation and boosted cell apoptosis in NALM-6 and RS4;11 cells. The subsequent investigation confirmed that LncRNA CRNDE bound to miR-345-5p and negatively regulated miR-345-5p expression. The overexpression of miR-345-5p suppressed cell proliferation and boosted cell apoptosis in NALM-6 and RS4;11 cells. Further experiments revealed that miR-345-5p downregulated cyclic AMP response element-binding protein (CREB) expression by targeting its mRNA directly. CREB overexpression reversed the effect of miR-345-5p mimic on cell proliferation and apoptosis in NALM-6 and RS4;11 cells. Finally, in vivo experiments showed that LncRNA CRNDE knockdown prolonged the survival of mice xenotransplanted with NALM-6 cells. In conclusion, LncRNA CRNDE upregulated CREB expression by suppressing miR-345-5p, thus promoting cell proliferation and reducing cell apoptosis in BCP-ALL.

카드뮴과 아연 노출에 따른 야외종 장수깔따구(Chironomus plumosus)의 생태독성학적 반응 (Ecotoxicological Response of Cd and Zn Exposure to a Field Dominant Species, Chironomus plumosus)

  • 김원석;홍철;박기연;곽인실
    • 생태와환경
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    • 제52권3호
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    • pp.266-273
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    • 2019
  • 인간의 인위적 활동으로 인해 발생하는 중금속 중 카드뮴(Cd)과 아연(Zn)은 다양한 경로를 통해 하천으로 유입되어 서식생물에게 유해 영향을 준다. 본 연구에서는 도심 하천에서 우점하는 장수깔따구 (Chironomus plumosus)를 이용하여 Cd와 Zn 노출에 따른 생태독성학적 반응을 연구하였다. 생활사를 고려하여, 생존율, 성장율, 우화율, 성비와 하순기절 기형을 관찰하였다. 장수깔따구 생존율은 Cd와 Zn 노출에 따라 시간, 농도의존적인 경향을 나타냈다. 성장율은 Cd 노출 후 Day 4와 Day 7, 농도의존적인 감소를 보였으며, 고농도인 $50mg\;L^{-1}$에서 대조군에 비해 급격한 감소를 나타냈다. 또한, 우화율은 10, $50mg\;L^{-1}$ Cd에서 대조군에 비해 감소함을 관찰하였다. 성비 변화는 $100mg\;L^{-1}$ Zn에서 암컷의 비율이 증가였으나 농도가 높아짐에 따라 수컷의 비율이 높아짐을 보이며 성비불균형이 관찰되었다. 게다가, 장수깔따구의 하순기절에서는 대조군에 비해 Cd와 Zn 노출된 장수깔따구에서 기형이 관찰되었다. 이와 같은 결과는 수생태계로 유입되는 중금속이 하천의 하상저층에 서식하는 저서무척추동물에게 유해한 영향을 주며, 중금속 노출에 따른 생물학적 분석을 위한 현장 지표종으로서의 가능성을 보여주었다.

재생에너지원 보급에 따른 전력계통 안정도 분석 (Analysis of Power System Stability by Deployment of Renewable Energy Resources)

  • 곽은섭;문채주
    • 한국전자통신학회논문지
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    • 제16권4호
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    • pp.633-642
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    • 2021
  • 제한적인 탄소배출의 필요성과 연계되는 전력수요 증가는 재생에너지산업에서 폭발적인 상승을 가져오고 있다. 전력계통에서 전기공급은 항상 전기수요와 균형을 맞추어야 할 필요가 있고, 안전하고 믿을만한 안정적인 운영을 유지하기 위하여 계통손실이 발생한다. 높은 비율의 재생에너지 보급을 갖는 전력계통에서 과도 안정도, 미소신호 안정도 및 주파수 안정도와 같은 넓은 범위의 3가지의 난제가 있다. 과도안정도는 선로계전기 동작이나 발전기 탈락과 같은 장애에 대한 계통응답을 해석하는 것이다. 미소신호 안정도는 계통관성 저하에 따른 전압불안정, 주파수 급변, 전력진동 등이 발생가능한 계통에서 조그만 증분같은 작은 동요가 일어날 때 전력계통 동기를 유지하기 위한 계통의 기능이다. 주파수 안정도는 발전과 부하 사이에 심한 불균형이 발생하는 중대 계통혼란에서도 정상 주파수를 유지하기 위한 전력계통의 기능으로 간주한다. 본 논문에서 재생에너지 보급계획에 따른 계통모의를 수행하여 3종류 안정도를 검토하며, 또한 재생에너지원이 계통안정도에 미치는 영향을 분석한다.

Green perilla leaf extract ameliorates long-term oxidative stress induced by a high-fat diet in aging mice

  • Edward, Olivet Chiamaka;Thomas, Shalom Sara;Cha, Kyung-Ok;Jung, Hyun-Ah;Han, Anna;Cha, Youn-Soo
    • Nutrition Research and Practice
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    • 제16권5호
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    • pp.549-564
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    • 2022
  • BACKGROUND/OBJECTIVES: Oxidative stress is caused by an imbalance between harmful free radicals and antioxidants. Long-term oxidative stress can lead to an "exhausted" status of antioxidant defense system triggering development of metabolic syndrome and chronic inflammation. Green perilla (Perilla frutescens) is commonly used in Asian cuisines and traditional medicine in southeast Asia. Green perilla possesses numerous beneficial effects including anti-inflammatory and antioxidant functions. To investigate the potentials of green perilla leaf extract (PE) on oxidative stress, we induced oxidative stress by high-fat diet (HFD) in aging mice. MATERIALS/METHODS: C57BL/6J male mice were fed HFD continuously for 53 weeks. Then, mice were divided into three groups for 12 weeks: a normal diet fed reference group (NDcon), high-fat diet fed group (HDcon), and high-fat diet PE treated group (HDPE, 400 mg/kg of body weight). Biochemical analyses of serum and liver tissues were performed to assess metabolic and inflammatory damage and oxidative status. Hepatic gene expression of oxidative stress and inflammation related enzymes were evaluated by quantitative real-time polymerase chain reaction (qRT-PCR). RESULTS: PE improved hepatopathology. PE also improved the lipid profiles and antioxidant enzymes, including hepatic glutathione peroxidase (GPx) and superoxide dismutase (SOD) and catalase (CAT) in serum and liver. Hepatic gene expressions of antioxidant and anti-inflammatory related enzymes, such as SOD-1, CAT, interleukin 4 (IL-4) and nuclear factor erythroid 2-related factor (Nrf2) were significantly enhanced by PE. PE also reduced the levels of hydrogen peroxide (H2O2) and malondialdehyde (MDA) in the serum and liver; moreover, PE suppressed hepatic gene expression involved in pro-inflammatory response; Cyclooxygenase-2 (COX-2), nitric oxide synthase (NOS), interleukin 1 beta (IL-1β), and interleukin 6 (IL-6). CONCLUSIONS: This research opens opportunities for further investigations of PE as a functional food and possible anti-aging agent due to its attenuative effects against oxidative stress, resulting from HFD and aging in the future.

동북아 부가가치 석유물류 비즈니스 모델에 관한 연구 (A Study on the Business Model for Value Added Petroleum Logistics in Northeast Asia)

  • 박지웅;이충배
    • 한국항만경제학회지
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    • 제39권1호
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    • pp.149-172
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    • 2023
  • 한·중·일을 포함하는 동북아지역은 1990년대 이후 급속한 경제성장과 더불어 역내외 석유물류가 증대되고 있다. 우리나라는 이러한 대외적인 환경하에서 2000년대 중반 이후 동북아 석유물류허브화 정책을 추진해 왔다. 동북아 물류허브화를 위해서는 부가가치 석유물류의 비즈니스 활성화를 위한 비즈니스 모델의 구축과 촉진이 중요하다. 동북아 지역에서 우리나라 석유물류 비즈니스 모델을 분석함으로써 석유물류 활성화에 대한 정책적·실무적 시사점을 제시하는 데 목적이 있다. 전체 23개 탱키지 업체를 대상으로 면담조사에 의한 사례분석결과는 다음과 같다. 첫째, 면담조사한 대부분의 유류저장탱크터미널업체들은 블랜딩, 소분화, 집적화와 같은 부가가치 석유물류를 실시하고 있다. 둘째, 부가가치 석유물류의 발생은 주변국들간 수급의 불균형에 의해 발생하고 있는데 특히 일본, 오세아니아, 미국, 남미 등을 연결하는 소분화와 블랜딩 수요가 높다. 셋째, 부가가치 물류수요에 대응한 인프라, 제도 및 규제 등에 대한 개선을 통해 부가가치 석유물류의 활성화를 촉진해야 할 것이며 이는 우리나라의 동북아 오일허브 정책에 크게 기여하게 될 것이다.

포아송 및 음이항 회귀분석을 이용한 해상운임 결정요인이 해운선사의 블랭크 세일링에 미치는 영향 분석 연구 (A Study on Impact of Factors Influencing Maritime Freight Rates Using Poisson and Negative Binomial Regression Analysis on Blank Sailings of Shipping Companies)

  • 류원형;남형식
    • 한국항해항만학회지
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    • 제48권1호
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    • pp.62-77
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    • 2024
  • 해상운송 산업에서는 공급과 수요의 불균형이 지속적으로 증가하면서 세계 주요 해운선사들이 해운 시황에 따른 선복량을 탄력적으로 조절하기 위해 블랭크 세일링을 주요 수단으로 사용하고 있다. 일반적으로 블랭크 세일링은 중국의 춘절 기간에 맞추어 많이 실시되어 왔지만, 2020년부터 시작된 글로벌 팬데믹과 미국·중국 간 무역 전쟁 등과 같은 특수한 상황으로 인해 최근 해운선사들은 기존 대비 큰 규모의 블랭크 세일링을 실시하였다. 이러한 블랭크 세일링은 화물 운송 지연에 직접적 영향을 미치기 때문에 기업과 소비자의 측면에서 부정적인 영향을 초래할 수 있다. 따라서 본 연구는 이에 선제적으로 대응하기 위해 포아송 회귀모형과 음이항 회귀모형을 활용하여 해상운임 결정요인이 해운선사의 블랭크 세일링에 미치는 영향력을 분석하였다. 분석 결과, 포아송 회귀분석의 2M의 경우 유의한 변수로 글로벌 컨테이너 해상물동량, 컨테이너 선복량, 컨테이너선 해체량, 컨테이너선 신조선가지수, OECD 인플레이션을 도출하였고, 음이항 회귀분석의 Ocean Alliance의 경우 글로벌 컨테이너 해상물동량과 컨테이너선 발주량을, THE Alliance의 경우 컨테이너선 선복량과 금리를, Non-Alliance의 경우 국제유가, 글로벌 공급망 압력지수, 컨테이너선 선복량, OECD 인플레이션을, Total Alliance의 경우 컨테이너선 선복량과 금리를 유의한 변수로 도출할 수 있었다.

견관절부 외상후 발생된 Shoulder-Hand Syndrome (A Case of the Shoulder-Hand Syndrome Caused by a Crush Injury of the Shoulder)

  • 전재수;이성근;송후빈;김선종;박욱;김성열
    • The Korean Journal of Pain
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    • 제2권2호
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    • pp.155-166
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    • 1989
  • Bonica defined, that reflex sympathetic dystrophy (RSD) may develop pain, vasomotor abnoramalities, delayed functional recovery, and dystrophic changes on an affected area without major neurologic injury following trauma, surgery or one of several diseased states. This 45 year old male patient had been crushed on his left shoulder by a heavily laden rear car, during his job street cleaning about 10 years ago (1978). At first the pain was localizea only to the site of injury, but with time, it spreaded from the shoulder to the elbow and hand, with swelling. X-ray studies in the local clinic, showed no bone abnormalities of the affected site. During about 10 years following the injury, the had recieved several types of treatments such as nonsteroidal analgesics, steroid injections into the glenoidal cavity (10 times), physical therapy, some oriental herb medicines, and acupuncture over a period of 1~3 months annually. His shoulder pain and it's joint dysfunction persisted with recurrent paroxysmal aggrevation because of being mismanaged or neglected for a sufficiently long period these fore permiting progression of the sympathetic imbalance. On July 14 1988 when he visited our clinic. He complained of burning, aching and had a hyperpathic response or hyperesthesia in touch from the shoulder girdle to the elbow and the hand. Also the skin of the affected area was pale, cold, and there was much sweating of the axilla and palm, but no edema. The shoulder girdle was unable to move due to joint pain with marked weakness. We confirmed skin temperatures $5^{\circ}C$ lower than those of the unaffected axilla, elbow and palm of his hand, and his nails were slightly ridged with lateral arching and some were brittle. On X-ray findings of both the shoulder AP & lateral view, the left humerus and joint area showed diffuse post-traumatic osteoporosis and fibrous ankylozing with an osteoarthritis-like appearance. For evaluating the RSD and it's relief of pain, the left cervical sympathetic ganglion was blocked by injecting 0.5% bupivacaine 5 ml with normal saline 5 ml (=SGB). After 15 minutes following the SGB, the clinical efficacy of the block by the patients subjective score of pain intensity (=PSSPI), showed a 50% reduction of his shoulder and arm pain, which was burning in quality, and a hyperpathic response against palpation by the examiner. The skin temperatures of the axilla and palm rose to $4{\sim}5^{\circ}C$ more than those before the SGB. He felt that his left face and upper extremity became warmer than before the SGB, and that he had reduced sweating on his axilla and his palm. Horner's sign was also observed on his face and eyes. But his deep shoulder joint pain was not improved. For the control of the remaining shoulder joint pain, after 45 minutes following the SGB, a somatic sensory block was performed by injecting 0.5% bupivacaine 6 ml mixed with salmon calcitonin, $Tridol^{(R)}$, $Polydyn^{(R)}$ and triamcinolone into the fossa of the acromioclavicular joint region. The clinical effect of the somatic block showed an 80% releif of the deep joint pain by the PSSPI of the joint motion. Both blocks, as the above mentioned, were repeated a total of 28 times respectively, during 6 months, except the steroid was used just 3 times from the start. For maintaining the relieved pain level whilst using both blocks, we prescribed a low dose of clonazepam, prazocin, $Etravil^{(R)}$, codeine, etodolac micronized and antacids over 6 months. The result of the treatments were as follows; 1) The burning, aching and hyperpathic condition which accompanied with vaosmotor and pseudomotor dysfunction, disappeared gradually to almost nothing, within 3 weeks from the starting of the blocks every other day. 2) The joint disability of the affected area was improved little by little within 6 months. 3) The post-traumatic osteoporosis, fibrous ankylosis and marginal sclerosis with a narrowed joint, showed not much improvement on the X-ray findings (on April 25, 1989) 10 months later in the follow-up. 4) Now he has returned to his job as a street cleaner.

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