Browse > Article
http://dx.doi.org/10.14348/molcells.2020.0065

LncRNA CRNDE Promotes the Progression of B-cell Precursor Acute Lymphoblastic Leukemia by Targeting the miR-345-5p/CREB Axis  

Wang, Weimin (Department of Hematology, The First Affiliated Hospital of Zhengzhou University)
Wu, Feifei (Department of Hematology, The First Affiliated Hospital of Zhengzhou University)
Ma, Ping (Department of Hematology/Oncology, Children's Hospital Affiliated of Zhengzhou University)
Gan, Silin (Department of Hematology, The First Affiliated Hospital of Zhengzhou University)
Li, Xue (Department of Hematology, The First Affiliated Hospital of Zhengzhou University)
Chen, Li (Department of Hematology, The First Affiliated Hospital of Zhengzhou University)
Sun, Ling (Department of Hematology, The First Affiliated Hospital of Zhengzhou University)
Sun, Hui (Department of Hematology, The First Affiliated Hospital of Zhengzhou University)
Jiang, Zhongxing (Department of Hematology, The First Affiliated Hospital of Zhengzhou University)
Guo, Feng (Department of Endocrinology, The First Affiliated Hospital of Zhengzhou University)
Publication Information
Molecules and Cells / v.43, no.8, 2020 , pp. 718-727 More about this Journal
Abstract
The imbalance between the proliferation and apoptosis of B-cell precursors is an important contributor to the pathogenesis of B-cell precursor acute lymphoblastic leukemia (BCP-ALL), while its specific regulatory mechanism remains perplexing. This study aimed to expound the underlying mechanism of the proliferation and apoptosis of BCP-ALL cells from the perspective of non-coding RNA. In this study, long non-coding RNA colorectal neoplasia differentially expressed (LncRNA CRNDE) was upregulated in the bone marrow of BCP-ALL patients and BCP-ALL cell lines (NALM-6 and RS4;11). Functionally, LncRNA CRNDE knockdown restrained cell proliferation and boosted cell apoptosis in NALM-6 and RS4;11 cells. The subsequent investigation confirmed that LncRNA CRNDE bound to miR-345-5p and negatively regulated miR-345-5p expression. The overexpression of miR-345-5p suppressed cell proliferation and boosted cell apoptosis in NALM-6 and RS4;11 cells. Further experiments revealed that miR-345-5p downregulated cyclic AMP response element-binding protein (CREB) expression by targeting its mRNA directly. CREB overexpression reversed the effect of miR-345-5p mimic on cell proliferation and apoptosis in NALM-6 and RS4;11 cells. Finally, in vivo experiments showed that LncRNA CRNDE knockdown prolonged the survival of mice xenotransplanted with NALM-6 cells. In conclusion, LncRNA CRNDE upregulated CREB expression by suppressing miR-345-5p, thus promoting cell proliferation and reducing cell apoptosis in BCP-ALL.
Keywords
B-cell precursor acute lymphoblastic leukemia; cyclic AMP-binding protein; long non-coding RNA colorectal neoplasia differentially expressed; miR-345-5p;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Chen, Q.G., Zhou, W., Han, T., Du, S.Q., Li, Z.H., Zhang, Z., Shan, G.Y., and Kong, C.Z. (2016). MiR-345 suppresses proliferation, migration and invasion by targeting Smad1 in human prostate cancer. J. Cancer Res. Clin. Oncol. 142, 213-224.   DOI
2 Cho, E.C., Mitton, B., and Sakamoto, K.M. (2011). CREB and leukemogenesis. Crit. Rev. Oncog. 16, 37-46.   DOI
3 Graham, L.D., Pedersen, S.K., Brown, G.S., Ho, T., Kassir, Z., Moynihan, A.T., Vizgoft, E.K., Dunne, R., Pimlott, L., Young, G.P., et al. (2011). Colorectal neoplasia differentially expressed (CRNDE), a novel gene with elevated expression in colorectal adenomas and adenocarcinomas. Genes Cancer 2, 829-840.   DOI
4 Gu, Y.Y., Chen, M.H., May, B.H., Liao, X.Z., Liu, J.H., Tao, L.T., Man-Yuen Sze, D., Zhang, A.L., and Mo, S.L. (2018). Matrine induces apoptosis in multiple colorectal cancer cell lines in vitro and inhibits tumour growth with minimum side effects in vivo via Bcl-2 and caspase-3. Phytomedicine 51, 214-225.   DOI
5 Han, S., Han, B., Li, Z., and Sun, D. (2019). Downregulation of long noncoding RNA CRNDE suppresses drug resistance of liver cancer cells by increasing microRNA-33a expression and decreasing HMGA2 expression. Cell Cycle 18, 2524-2537.   DOI
6 Hassan, M., Watari, H., AbuAlmaaty, A., Ohba, Y., and Sakuragi, N. (2014). Apoptosis and molecular targeting therapy in cancer. Biomed Res. Int. 2014, 150845.   DOI
7 Hu, C.E., Du, P.Z., Zhang, H.D., and Huang, G.J. (2017). Long noncoding RNA CRNDE promotes proliferation of gastric cancer cells by targeting miR-145. Cell. Physiol. Biochem. 42, 13-21.   DOI
8 Jacobson, S., Tedder, M., and Eggert, J. (2016). Adult acute lymphoblastic leukemia: a genetic overview and application to clinical practice. Clin. J. Oncol. Nurs. 20, E147-E154.   DOI
9 Johannessen, M., Delghandi, M.P., and Moens, U. (2004). What turns CREB on? Cell. Signal. 16, 1211-1227.   DOI
10 James, A.R., Schroeder, M.P., Neumann, M., Bastian, L., Eckert, C., Gokbuget, N., Tanchez, J.O., Schlee, C., Isaakidis, K., Schwartz, S., et al. (2019). Long non-coding RNAs defining major subtypes of B cell precursor acute lymphoblastic leukemia. J. Hematol. Oncol 12, 8.   DOI
11 Kang, X., Wang, M., Wang, H., Shen, X., and Guan, W. (2016). MiR-4282 suppresses proliferation and mobility of human colorectal carcinoma cells by targeting semaphorin 3E. Panminerva Med. 58, 197-205.
12 Lajoie, M., Drouin, S., Caron, M., St-Onge, P., Ouimet, M., Gioia, R., Lafond, M.H., Vidal, R., Richer, C., Oualkacha, K., et al. (2017). Specific expression of novel long non-coding RNAs in high-hyperdiploid childhood acute lymphoblastic leukemia. PLoS One 12, e0174124.   DOI
13 Liu, X., Gan, L., and Zhang, J. (2019). miR-543 inhibites cervical cancer growth and metastasis by targeting TRPM7. Chem. Biol. Interact. 302, 83-92.   DOI
14 Shima, Y. and Kitabayashi, I. (2011). Deregulated transcription factors in leukemia. Int. J. Hematol. 94, 134-141.   DOI
15 Liu, X.X., Xiong, H.P., Huang, J.S., Qi, K., and Xu, J.J. (2017). Highly expressed long non-coding RNA CRNDE promotes cell proliferation through PI3K/AKT signalling in non-small cell lung carcinoma. Clin. Exp. Pharmacol. Physiol. 44, 895-902.   DOI
16 Salmena, L., Poliseno, L., Tay, Y., Kats, L., and Pandolfi, P.P. (2011). A ceRNA hypothesis: the Rosetta Stone of a hidden RNA language? Cell 146, 353-358.   DOI
17 Shabestari, R.M., Safa, M., Alikarami, F., Banan, M., and Kazemi, A. (2017). CREB knockdown inhibits growth and induces apoptosis in human pre-B acute lymphoblastic leukemia cells through inhibition of prosurvival signals. Biomed. Pharmacother. 87, 274-279.   DOI
18 Avval, A.J., Majd, A., Gholipour, N., Noghabi, K.A., Ohradanova-Repic, A., and Ahangari, G. (2019). An inventive report of inducing apoptosis in non-small cell lung cancer (NSCLC) cell lines by transfection of miR-4301. Anticancer Agents Med. Chem. 19, 1609-1617.   DOI
19 Zhang, X., Zhou, J., Xue, D., Li, Z., Liu, Y., and Dong, L. (2019). MiR-515-5p acts as a tumor suppressor via targeting TRIP13 in prostate cancer. Int. J. Biol. Macromol. 129, 227-232.   DOI
20 Zhuang, Y., Li, D., Fu, J., Shi, Q., Lu, Y., and Ju, X. (2014). Overexpression of AIOLOS inhibits cell proliferation and suppresses apoptosis in Nalm-6 cells. Oncol. Rep. 31, 1183-1190.   DOI
21 Buske, C., Becker, D., Feuring-Buske, M., Hannig, H., Wulf, G., Schafer, C., Hiddemann, W., and Wormann, B. (1997). TGF-beta inhibits growth and induces apoptosis in leukemic B cell precursors. Leukemia 11, 386-392.   DOI
22 Cesana, M., Cacchiarelli, D., Legnini, I., Santini, T., Sthandier, O., Chinappi, M., Tramontano, A., and Bozzoni, I. (2011). A long noncoding RNA controls muscle differentiation by functioning as a competing endogenous RNA. Cell 147, 358-369.   DOI