• Journal of Microbiology and Biotechnology
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• v.16 no.1
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• pp.25-31
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• 2006

Changes of CP4 5-enolpyruvylshikimate-3-phosphate synthase (CP4 EPSPS) in the glyphosate-tolerant Roundup Ready soybean were examined using purified CP4 EPSPS produced in cloned Escherichia coli as a control. CP4 EPSPS in genetically modified soybean was detected by twodimensional gel electrophoresis (2-DE) and identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and electrospray ionization tandem mass spectrometry (ESI-MS/MS) with databases. CP4 EPSPS in soybean products was resolved on 2-DE by first isoelectric focusing (IEF) based on its characteristic pI of 5.1, followed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) based on its molecular mass of 47.5 kDa. We quantified various percentages of soybean CP4 EPSPS. The quantitative analysis was performed using a 2D software program on artificial gels with spots varying in Gaussian volumes. These results suggested that 2-DE image analysis could be used for quantitative detection of GM soybean, unlike Western blotting.

• Journal of Korea Multimedia Society
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• v.17 no.7
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• pp.818-828
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• 2014

After emerging of Microsoft Kinect, the interest in three-dimensional (3D) depth image was significantly increased. Depth image data of an object can be converted to 3D coordinates by simple arithmetic calculation and then can be reconstructed as a 3D model on computer. However, because the surface coordinates can be acquired only from the front area facing Kinect, total solid which has a closed surface cannot be reconstructed. In this paper, 3D registration method for multiple Kinects was suggested, in which surface information from each Kinect was simultaneously collected and registered in real time to build 3D total solid. To unify relative coordinate system used by each Kinect, 3D perspective transform was adopted. Also, to detect control points which are necessary to generate transformation matrix, 3D randomized Hough transform was used. Once transform matrices were generated, real time 3D reconstruction of various objects was possible. To verify the usefulness of suggested method, human arms were 3D reconstructed and the volumes of them were measured by using four Kinects. This volume measuring system was developed to monitor the level of lymphedema of patients after cancer treatment and the measurement difference with medical CT was lower than 5%, expected CT reconstruction error.

• Investigative Magnetic Resonance Imaging
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• v.21 no.4
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• pp.223-232
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• 2017

Purpose: To report the use of multiband accelerated echo-planar imaging (EPI) for resting-state functional MRI (rs-fMRI) to achieve rapid high temporal resolution at 3T compared to conventional EPI. Materials and Methods: rs-fMRI data were acquired from 20 healthy right-handed volunteers by using three methods: conventional single-band gradient-echo EPI acquisition (Data 1), multiband gradient-echo EPI acquisition with 240 volumes (Data 2) and 480 volumes (Data 3). Temporal signal-to-noise ratio (tSNR) maps were obtained by dividing the mean of the time course of each voxel by its temporal standard deviation. The resting-state sensorimotor network (SMN) and default mode network (DMN) were estimated using independent component analysis (ICA) and a seed-based method. One-way analysis of variance (ANOVA) was performed between the tSNR map, SMN, and DMN from the three data sets for between-group analysis. P < 0.05 with a family-wise error (FWE) correction for multiple comparisons was considered statistically significant. Results: One-way ANOVA and post-hoc two-sample t-tests showed that the tSNR was higher in Data 1 than Data 2 and 3 in white matter structures such as the striatum and medial and superior longitudinal fasciculus. One-way ANOVA revealed no differences in SMN or DMN across the three data sets. Conclusion: Within the adapted metrics estimated under specific imaging conditions employed in this study, multiband accelerated EPI, which substantially reduced scan times, provides the same quality image of functional connectivity as rs-fMRI by using conventional EPI at 3T. Under employed imaging conditions, this technique shows strong potential for clinical acceptance and translation of rs-fMRI protocols with potential advantages in spatial and/or temporal resolution. However, further study is warranted to evaluate whether the current findings can be generalized in diverse settings.

• Journal of the Korean Society of Radiology
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• v.16 no.1
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• pp.53-59
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• 2022

DEXA, as the standard areal bone mineral density (aBMD) measurement method, often shows an insuficient correlation between aBMDs of the measured bones and referring bones and is inaccurate due to the mass effect. In contrast, quantitative computer tomography (QCT), as a volumetric BMD (vBMD) measurement method, is being advanced so that it uses less radiation before, owing to improved CT device and computer imaging technology. Because dual-energy CTs can modulate the image signals showing tumor or specific chemicals as well as the ability to measure vBMD, they are expanding their application. For pre-checking vBMD of surgeon-specific bone volume at implantation candidate sites, a finite element creation-based local vBMD measurement technique was developed. The local vBMD measurement function for surgeon-specific shape volumes will be added to clinical imaging systems.

• Korean Journal of Digital Imaging in Medicine
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• v.3 no.1
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• pp.76-84
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• 1997

We tried to find any factor to reduce the exposure dose with ST-VA type generator which has the higher exposure dose. The guideline recommended by the provisions of IAEA regulation is being used at the time of radiation exposure and almost of those equipments used for this study had less amount of doses than those in the guideline. The FCR may be possible to decrease a certain amount of dose with effective applications of it's automatic image compensation utilities and the verifying function in PACS. Next time, it is required to add a great number of hospitals for wide range of studios and measurements, and make standard exposure Control table which will bing in practice, material decrease of dose volumes when radiation exposure.

• Journal of Biomedical Engineering Research
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• v.7 no.2
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• pp.169-182
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• 1986

Pulsed ultrasonic Doppler system is a useful diagnostic instrument to measure blood-flow-velocity, velocity profile, and volume-blood-flow. This system is more powerful compare with 2-dimensional B-scan tissue image. A system has been deve- loped and ii being evaluated using TMS 32010 DSP. We use this DSP for the purpose of real-time spectrum analyzer to obtain spectrogram in singlegate pulsed Doppler system and for the serial comb filter to cancel clutter and zero crossing counter to estimate Doppler mean frequency in multigate pulsed Doppler system. The Doppler shift of the backscattered signals is sensed in a phase detector. This Doppler signal corresponds to the mean velocity over a some region in space defined by the ultrasonic beam dimensions, transmitted pulse duration, and transducer ban(iwidth. Multi- gate pulsed Doppler system enable the transcutaneous and simultaneous assessment of the velocities in a number of adjacent sample volumes as a continuous function of time. A multigate pulsed Doppler system processing the information originating from presented.

• Korean Journal of Computational Design and Engineering
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• v.13 no.6
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• pp.469-477
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• 2008

Physically-based rendering is an image synthesis technique based on simulation of physical interactions between light and surface materials. Since generated images are highly photorealistic, physically-based rendering has become an indispensable tool in advanced design visualization for manufacturing and architecture as well as in film VFX and animations. Especially, BRDF (bidirectional reflectance distribution function) is critical in realistic visualization of materials since it models how an incoming light is reflected on the surface in terms of intensity and outgoing angles. In this paper, we introduce techniques to represent BRDF as B-spline volumes and to utilize them in physically-based rendering. We show that B-spline volume BRDF (BVB) representation is suitable for measured BRDFs due to its compact size without quality loss in rendering. Moreover, various CAGD techniques can be applied to B-spline volume BRDFs for further controls such as refinement and blending.

• Proceedings of the KSRS Conference
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• 2007.10a
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• pp.620-624
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• 2007

Recently, Gonzalez-Audicana et al. proposed the substitute wavelet intensity (SWI) method which provided a solution based on the intensity-hue-saturation (IHS) method for the fusing of panchromatic (PAN) and multispectral (MS) images. Although the spectral quality of the fused MS images is enhanced, this method is not efficient enough to quickly merge massive volumes of data from satellite. To overcome this problem, we introduce a new SWI method based on a fast IHS transform to implement efficiently as an alternative procedure. In addition, we show that the method is well applicable for fusing IKONOS PAN with MS images.

• Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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• 2003.04a
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• pp.180-183
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• 2003

Recently, the pollution of soil and groundwater becomes a serious social problem, and geophysical exploration methods have been introduced as a remedial investigation method of subsurface. Digital technologies such as personal computer have revolutionized our ability to acquire large volumes of data in a short term, and to produce more reliable results for subsurface image. Also, color graphics easily visualizes the survey results in a more understandable manner, and it is widely used for not only characterizing the contaminated subsurface but also monitoring contaminant and remedial process. In this paper, electrical resistivity survey were carried out In order to understand characteristics of waste landfills, and the applicability of geophysical prospecting to site assessment of waste landfill was also tested. According to the result, electrical resistivity survey were effective in estimating distribution of the leachate plume.

• Journal of Biomedical Engineering Research
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• v.12 no.4
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• pp.295-302
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• 1991

In this paper, the inter- and intra-frame distortions in the gray levels of a series of fluorescein ocular fundus photographs are corrected. For doing this, the background images are extracted from original images using the image blurring effect by decimation, and then shading corrected images are obtained by subtracting the background images from the original images pixel by pixel. In a series of fluorescein ocular fundus photographs, after the gray scale distoriton is corrected, the intensity volumes of dye leakage are measured and represented by a graph. These data may be useful for the prediction of prognosis and the therapeutic management.