• Journal of Biosystems Engineering
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• 제38권4호
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• pp.335-340
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• 2013

Purpose: This study was performed to develop a lateral flow strip sensor for the detection of pathogenic Escherichia coli O157:H7 in various samples. Also, feasibility of using an image analysis method to improve the interpretation of the strip sensor was evaluated. Methods: The lateral flow strip sensor has been fabricated based on nitrocellulose lateral-flow membrane. Colloidal gold and E. coli O157:H7 antibodies were used as a tag and a receptor, respectively. Manually spotted E. coli O157:H7 antibody and anti-mouse antibody on nitrocellulose membrane were used as test and control dots, respectively. Feasibility of the lateral flow strip sensor to detect E. coli O157:H7 were evaluated with serially diluted E. coli O157:H7 cells in PBS or food samples. Test results of the lateral flow strip sensor were measured with an image analysis method. Results: The intensity of the test dot started to increase with higher concentration of the cells were introduced. The sensitivities of the sensor were both $10^4$ CFU/mL Escherichia coli O157:H7 spiked in PBS and in chicken meat extract, respectively. Conclusions: The lateral flow strip sensor and image analysis method could detect E. coli O157:H7 in 20 min, which is significantly quicker than conventional plate counting method.

• 한국실내디자인학회논문집
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• 제22호
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• pp.61-68
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• 2000

This paper illustrates what the reality of architectural concepts, materials, notions and phenomena are as central questions of contemporay space design. These issues form the goal of modern space design which should attain to the reality of an era when non-real values prevail. Despite the trends of an everchanging and ephemeral dominating quality in architecture for the last ten years, architects still aim to contruct everlasting space on earth. The trends of dematerialization in today's space design can be substantiated in spatial-temporal dimensions as follows. First of all, ephemeral architecture with concepts of hypothetical temporality, everchanging architecture in fluidity, and the transparent architecture with the floating and overlapping image can be analyzed in the dimension of 'time'. In terms of 'space', void quality for the expression of emptiness, neutral space by the simplified and summarized forms, expanded space through ambiguous boundaries and spatial repetition can becharacterized and also be intended strategies for lightness, state of flux, ambiguity, paradox etc., lead modern space design along that path. As this point, we need to pay attention to the so-called 'hypersurface' concept proposed by Stephen Perella. Hypersurface is a sort of cladding sheathing existence independent from the primary structure. With it, the integration between form and image can be achieved. Sometimes hypersurface can be a strategical screen for image projection, a cognitive receptor for surroundings as well as a catalyst for information and communication systems. When the situation dematerializes more and more as the years go on, the concept of hypersurface can be an inclusive method between the phenomenological form in architecture and its self recipient image. Permissive atmospheres created between them in contemporary space design and new paradigms emerged with digital technology will further reinforce the human space's dynamism.

• 전자공학회논문지
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• 제50권12호
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• pp.247-253
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• 2013

본 연구의 목적은 Direct DR(Digital Radiography), Indirect DR, I.I(Image Intensifier) DR에서 X선 광자 검출 방식에 따른 선량측정 및 획득된 영상을 정량적이고 객관적인 측정을 통해 DR System을 비교 평가 하는 것이다. Rando phantom을 사용하여 입사표면선량을 측정하였으며, 측정된 입사표면선량 값을 통해 PCXMC 프로그램을 사용하여 유효선량과 방사선 조사로 인한 위험을 평가하였다. 21cm 아크릴 phantom을 사용하여 SNR(Signal to Noise Ratio), NPS(Noise Power Spectrum), CNR(Contrast to Noise Ratio)을 측정하였으며, 측정값은 통계학적 분석기법을 사용하여 유의성을 평가하였다. 입사표면선량, 주요장기선량, 유효선량 모두 direct DR이 가장 낮게 측정되었으며, direct DR 선량을 기준으로 I.I type DR은 약 1.3배, indirect DR은 약 2.4배 높은 선량 비율로 측정되었다. 방사선량에 따른 위험도 역시 동일한 비율로 측정되었다. SNR 측정 결과 direct DR측정값을 기준으로 I.I DR은 약 7.25배, indirect DR이 약 1.48배 낮은 비율로 측정되었다. CNR 측정 결과 direct DR 측정값을 기준으로 I.I DR은 약 1.16배 높고, indirect DR이 약 0.87배 낮은 비율로 측정되었다. 따라서 a-selenium 검출소자를 사용하여 X선 광자를 검출하는 방식인 direct DR은 적은 선량으로 우수한 화질의 영상을 구현함으로써 선량에 민감한 소아나 생식선이 포함된 검사 등에 유용할 것으로 사료된다. 또한 많은 진단 정보를 위한 영상 평가가 요구되는 경우에는 indirect DR이 유용할 것으로 판단된다.

• 대한방사선기술학회지:방사선기술과학
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• 제44권4호
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• pp.295-300
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• 2021

This study aims to present new chest AP examination exposure conditions through a study on the effect on image quality and patient dose by applying high tube voltage and scatter ray post-processing software during chest AP examination in digital radiography equipment. This study was used a human body phantom and in the chest AP position, the dosimeter was placed horizontally at the thoracic spine 6. The experiment was conducted by dividing into a low tube voltage (70 kVp, 400 mA, 3.2 mAs) group and a high tube voltage (100 kVp, 400 mA, 1.2 mAs) group. The collimation size (14″× 17″) and the source to image receptor distance(110 cm) were same applied to both groups. Radiation dose was presented to dose area product and entrance surface dose. Image quality was compared and analyzed by comparing the difference between the signal-to-noise ratio and the contrast-to-noise ratio of the image according to the application of the scatter ray post-processing software under each condition. The average value of the entrance surface dose in the low and high tube voltage conditions was 93.04±0.45 µGy and 94.25±1.51 µGy, which was slightly higher in the high tube voltage condition, but the dose area product was 0.97±0.04 µGy and 0.93±0.01 µGy. There was a statistically significant difference in the group mean value(p<0.01). In terms of image quality, the values of the signal-to-noise ratio and the contrast noise ratio were higher in the high tube voltage than in the low tube voltage, and decreased when the scattering line post-processing function was used, but the contrast resolution was improved. If there is a scatter ray post-processing function during chest AP examination, it is helpful to actively utilize it to improve the image quality. However, when this function is not available, I thought that applying a higher tube voltage state than a low tube voltage state will help to realize images with a large amount of information without changing the dose.

• 대한방사선기술학회지:방사선기술과학
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• 제46권4호
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• pp.287-294
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• 2023

This study was conducted to ultimately reduce unnecessary radiation exposure by emphasizing the need and importance of correct positioning by examining the positioning relationship of anatomical structures in the human body and changes in X-ray images according to changes in patient positioning during the left lateral chest X-ray examination. This study investigated and analyzed previously published papers and books on the left lateral chest X-ray examination to find out the importance of positioning in the left lateral chest X-ray examination. To find out the importance of correct positioning in the left lateral chest X-ray, we compared three images of incorrectly positioned right thorax and left thorax rotated forward and the lower median surface of the body leaning against the image receptor. In the left lateral chest examination, a distorted image was obtained in which the shape of the anatomical structure observed in the image was changed according to the presence or absence of rotation of the patient and the inclination of the median visual surface. X-ray images with the most accurate and large amount of information were obtained from X-ray images with the correct positioning performed during left lateral chest X-ray examination. Therefore, It is believed that the left lateral chest X-ray examination will have beneficial effects such as providing accurate medical information, preventing misdiagnosis, reducing social costs, and ultimately reducing radiation exposure.

• 한국응용약물학회:학술대회논문집
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• 한국응용약물학회 1994년도 춘계학술대회 and 제3회 신약개발 연구발표회
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• pp.287-287
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• 1994

In the present study, it was aimed to asses the possibility that calcitonin gene-related peptide (CGRP) released in response to transient hypotension may contribute to the reflex autoregulation of cerebral blood flow as a putative modulator. Changes in pial arterial diameter (mean, 33.0 ${\pm}$ 1.1 $\mu\textrm{m}$) with changes in systemic arterial blood pressure (mean, 101.9 ${\pm}$ 2.7 mmHg) were observed directly through a closed cranial window in anesthetized normotensive rats. Image of the pial vessels was captured with a stereoscope connected to a CCD video camera and the diameter was measured with a microscaler. In the capsaicin-treated rats (one day prior to experiment, 50 nmol capsaicin injected intracisternally), both vasodilater and vasoconstrictor responses evoked by a transient hypotension and the reverse of blood pressure were markedly attenuated or almost abolished. When changes in pial arterial diameter were plotted as a function of changes in blood pressure, the slopes of both regression lines (for vasodilators and vasoconstrictors ) were markedly reduced. Similar reductions were evidenced under treatment wi th the CGRP antibody serum (1:1,000) and following CGRP receptor desensitization. However, the autoregulatory mechanics were neither affected by treatment wi th spantide (1 ${\mu}$M), substance P antagonist, nor by substance P receptor desensitization. Suffusion wi th mock cerebrospinal fluid containing CGRP and cromakalim caused a vasodilatation in a concentration-dependent manner, respectively and their effects were antagonized by glibenclamide. Substance P produced a vasodilatation, which was, however, little affected by glibenclamide. These observations indicate that the CGRP released from the perivascular sensory fibers in response to a hypotension is implicated in the modulation of the autoregulation of cerebral blood flow.

• Journal of Radiation Protection and Research
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• 제38권1호
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• pp.29-36
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• 2013

CR과 DR 장비에서 팬텀을 이용하여 선량과 노출지수(exposure index, EI)의 변화를 관찰하고 처리된 영상에서 NSR(noise to signal ratio)과 CNR (contrast to noise ratio)을 측정하여 디지털 시스템에서 방사선량을 최적화하기 위한 인자들 간의 상관관계를 알아보고자 하였다. EI와 입사표면선량(entrance surface dose, ESD)는 CR과 DR 장비에서 모두 주어진 선량의 증가에 따라 비례하여 증가하였다. 적정범위 내 EI 산출시 CR의 경우 DR 보다 더 많은 선량이 요구되었으나 두 시스템에서 모두 EI 지표는 노출인자인 kVp, mAs의 증가에 대해 선형으로 비례하여 증가하였다. 특히, 검출기 효율이 우수한 DR 시스템에서는 선량 변화에 대해 더 안정된 감도를 나타내었고, 최소의 선량증가에도 영상의 유용한 대조도를 형성하여 화질의 향상에 쉽게 도달할 수 있었다. 이는 검출기 흡수선량과 밀접한 관련이 있는 EI 지표의 정확성을 예측 가능하게 하였다. EI의 물리적 특징과 영상평가를 위한 NSR 측정은 DR 시스템에서 CR 보다 더 낮은 NSR이 나타났으며 CR에서는 6 mAs이상의 관전류에서는 관전압의 값에 따른 영향이 상대적으로 적었다. 본 연구 결론은 디지털 시스템 검출기의 흡수선량 측정의 지표인 EI는 노출인자와 선형비례관계에 있었고 EI가 제조사에서 제공한 범위 내에 존재할 때 그 EI 지표가 우수할수록 최적의 영상화질을 얻을 수 있었다. 또한 디지털 방사선 영상 기술에서 EI의 물리적 특징의 활용은 실제 임상에서 영상의 화질향상에 도움을 주고 검출기의 품질관리를 통하여 환자의 불필요한 피폭선량을 줄이는 데 많은 기여를 할 수 있을 것이다.

• 한국방사선학회논문지
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• 제10권6호
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• pp.381-385
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• 2016

본 연구에서는 공극 기법의 고관전압 촬영에서 격자를 사용한 것과 같은 화질을 유지하면서 선량을 줄이는 방안을 제안하고자 하였다. 실험은 초점과 수용체와의 거리 180 cm에서 공극을 10 cm, 15 cm, 20 cm, 25 cm, 30 cm으로 하였으며 각각의 공극 거리에서 관전류를 15 mAs로 고정하고 관전압은 80 KvP, 85 kVp, 90 kVp, 95 kVp, 100 kVp로 하여 촬영을 하였다. 전통적인 방법인 격자를 사용하였을 때 촬영은 초점과 수용체와의 거리 180 cm에서 15 mAs, 107 kVp로 하였다. 실험결과 격자를 사용하여 촬영하였을 때 표면선량은 0.130 R로 나타났고 공극을 20 cm로 하였을 때의 표면선량은 0.124 R로 나타났으며 두 영상 간의 PSNR은 10.65 [dB]로 나타났다. 결론적으로 표면선량이 적으면서 산란선을 제거하여 격자를 사용하였을 때와 유사한 화질을 유지할 수 있는 공극의 거리는 20 cm로 나타났다. 연구의 결과는 공극을 이용한 방사선촬영에서 표면선량을 제거할 수 있는 지표로 사용할 수 있을 것으로 사료된다. 결론적으로 표면선량이 적으면서 산란선을 제거하여 격자를 사용하였을 때와 유사한 화질을 유지할 수 있는 공극의 거리는 20 cm로 나타났다. 연구의 결과는 공극을 이용한 방사선촬영에서 표면선량을 절감할 수 있는 지표로 사용할 수 있을 것으로 사료된다.

• Journal of Gastric Cancer
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• 제21권2호
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• pp.191-202
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• 2021

Purpose: A near-infrared (NIR) fluorescence imaging is a promising tool for cancer-specific image guided surgery. Human epidermal receptor 2 (HER2) is one of the candidate markers for gastric cancer. In this study, we aimed to synthesize HER2-specific NIR fluorescence probes and evaluate their applicability in cancer-specific image-guided surgeries using an animal model. Materials and Methods: An NIR dye emitting light at 800 nm (IRDye800CW; Li-COR) was conjugated to trastuzumab and an HER2-specific affibody using a click mechanism. HER2 affinity was assessed using surface plasmon resonance. Gastric cancer cell lines (NCI-N87 and SNU-601) were subcutaneously implanted into female BALB/c nu (6-8 weeks old) mice. After intravenous injection of the probes, biodistribution and fluorescence signal intensity were measured using Lumina II (Perkin Elmer) and a laparoscopic NIR camera (InTheSmart). Results: Trastuzumab-IRDye800CW exhibited high affinity for HER2 (K_D=2.093(3) pM). Fluorescence signals in the liver and spleen were the highest at 24 hours post injection, while the signal in HER2-positive tumor cells increased until 72 hours, as assessed using the Lumina II system. The signal corresponding to the tumor was visually identified and clearly differentiated from the liver after 72 hours using a laparoscopic NIR camera. Affibody-IRDye800CW also exhibited high affinity for HER2 (K_D=4.71 nM); however, the signal was not identified in the tumor, probably owing to rapid renal clearance. Conclusions: Trastuzumab-IRDye800CW may be used as a potential NIR probe that can be injected 2-3 days before surgery to obtain high HER2-specific signal and contrast. Affibody-based NIR probes may require modifications to enhance mobilization to the tumor site.

• 대한방사선기술학회지:방사선기술과학
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• 제39권3호
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• pp.407-414
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• 2016

본 연구의 목적은 엑스선 선원과 검출기사이의 거리(SID)변화와 부가필터 사용 유무에 따른 피폭선량을 측정하여 환자선량을 최소화 하는 것이다. 연구를 위해 사용한 기기 및 팬텀은 DR시스템, 복부 조직 등가팬텀 그리고 알루미늄 필터를 사용하였다. 촬영 조건으로는 관전압을 80 kVp로 고정하고, 선량은 자동노출제어 장치를 통해 설정 하였다. 또한, 조사야는 $16{\times}16inch$를 사용하였다. 실험방법으로는 SID를 100 cm, 110 cm, 120 cm, 130 cm로 변화를 주어 각각 10회 측정하였고, 조사야 중심에 Piranha657 선량계를 위치시켰다. 촬영된 영상은 이미지J 통해 분석하였다. 실험 결과는 관전류량은 SID가 증가함에 따라 증가한 반면, 입사표면선량(ESD)은 SID가 증가함에 따라 급격히 감소하였다. 이는 낮은 에너지를 갖는 X선 광자들이 여과판사용으로 인해 제거되어 ESD의 감소로 기인한 것으로 사료되며, 이미지 J를 통한영상의 히스토그램 분석결과 SID 변화에 따라 ESD와 조사조건의 차이가 나타나지만 영상의 히스토그램상의 분포는 큰 차이는 없었다. 따라서, SID를 크게 할수록 환자의 선량을 줄일 수 있으며, 아울러 소아 등을 촬영할 때, 부가필터를 사용하면 환자선량을 더욱 줄일 수 있을 것이라 사료된다.