• 동의생리병리학회지
• /
• 제28권4호
• /
• pp.411-416
• /
• 2014

Simple formulas (單方) of muscle section in Donguibogam (東醫寶鑑) have long been prescribed for strengthening muscle and/or prevention of age-related muscle loss. However, biological activity and mechanisms by which they influence myoblast differentiation have not been studied. Therefore, in this study, we evaluated the effects of 14 simple formulas on myoblast differentiation in C2C12 myoblast cells under non-cytotoxic ($0.5mg/m{\ell}$) conditions. C2C12 cells were treated with water extracts of simple formulas for 72 h, and RT-PCR was performed to determine the gene expression levels of myogenic regulatory factors (MRFs), including myoD, myogenin, MRF4, myf5, and insulin like growth factor-1 (IGF-1). Treatment with Colocasiae Rhizoma (CR), Pini Semen (PS), and Sesami Semen (SS) resulted in a significant increase in expression of myogenin in C2C12 cells. Treatment with Allii Macrostemi Bulbus (AM), Colocasiae Rhizoma (CR), and Pini Semen (PS) also resulted in increased expression of MRF4 in C2C12 cells. In addition, enhanced expression of IGF-1 was observed in treatment with Eucommiae cortex (EC), Dioscoreae Rhizoma (DR), Colocasiae Rhizoma (CR), Pini Semen (PS), and Sesami Semen (SS) in C2C12 cells. These results indicate that simple formulas of muscle section in Donguibogam could potentially enhance myoblast differentiation at least in part via increasing expression of myogenin, and/or MRF4 and/or IGF-1.

• 한국축산식품학회지
• /
• 제25권3호
• /
• pp.316-321
• /
• 2005

본 시험은 육성-비육돈 사료내 인진쑥, 오가피 및 마늘을 건조시킨 분말 제제의 첨가에 따른 생산성, 혈청내 IGF-1 및 도체 특성에 미치는 영향에 대해 알아보고자 실시하였다. 개시시 체중 $20.92{\pm}2.13kg$의 3원 교잡종 [$Landrace{\times}Yorkshire{\times}Duroc$] 비육돈 96두를 공시하여 16주간 사양시험을 실시하였다. 시험설계는 옥수수-대두박 위주의 사료로서 1) CON(basal diet; Control), 2) MP1 (basal diet added $0.02\%$ of medicinal plant mixtures), 3) MP2 (basal diet added $0.04\%$ of medicinal plant mixtures) 및 4) MP3 (basal diet added $0.06\%$ of medicinal plant mixtures)의 4개의 처리구로 하여 처리당 6반복, 반복당 4두씩 완전임의 배치하였다. 전체 시험기간동안의 증체량에서는 첨가수준에 따라 증가되지는 않았지만 사료 섭취량 (linear, P<0.08)의 감소와 사료 효율 (linear, P<0.02; quadratic, P<0.08)의 향상이 첨가 수준이 증가할수록 개선되는 경향을 나타냈다. 등지방 두께에서는 약용 식물을 첨가함에 따라 감소하는 경향을 보였다 (linear, P<0.094; quadratic, P<0.011). 혈청내 IGF-1의 함량은 사료내 약용 식물의 첨가함에 따라 유의적으로 증가하는 경향을 보였다 (linear, P<0.090). Redness ($a^{*}-value$)와 yellowness ($b^{*}-value$)를 나타내는 $a^{*}$(linear, P<0.012)와 $b^{*}$(linear, P<0.018)에서는 약용 식물을 첨가함에 따라 증가하는 경향을 보였다. 또한, 돈육의 NPPC에 의한 검사 결과에서도 약용 식물의 첨가수준이 증가함에 따라 유의적으로 증가하는 경향을 보였다(linear, P<0.032). 이상의 결과를 종합하여 볼 때 육성-비육돈 사료내 약용 식물을 $0.06\%$까지 첨가하여 장기간 급여시 성장율, IGF-1 및 도체 특성 중 육색에 좋은 영향을 미칠 것으로 보인다.

• 농업과학연구
• /
• 제45권3호
• /
• pp.429-435
• /
• 2018

The aim of this study was to provide different levels of dietary protein for optimum growth performance in calves and to investigate the impact on the concentration of insulin-like growth factor-1 (IGF-1) in serum. Female Sahiwal calves (n = 12, body weight (BW) = $40{\pm}4.3kg$ and age = $60{\pm}10days$) were selected and divided into three groups with 4 animals in each group. Three diets of T0, T1 and T2 with 18, 20 and 22% of crude protein levels, respectively, were given to the calves to assess the post-weaning growth performance. Calves fed the T2 diet showed a higher (p < 0.05) dry matter (DM) intake than those fed the T0 and T1 diets. The feed conversion ratio (FCR) for the calves fed the T2 diet was lower (p < 0.05) than those of the calves fed the T0 and T1 diets. The T2 group had the highest BW (p < 0.05) compared with the other groups. The concentration of IGF-1 in serum increased (121.9, 143.3, and 152.9 ng/ml for T0, T1, and T2, respectively) as the crude protein (CP) level increased. Overall, the results of this study suggest that post weaning diets T1 and T2 with 20 and 22% crude protein in Sahiwal female calves had significantly increased the BW and serum IGF-1 concentration. The IGF-1 estimation might be a physiological indicator for growth performance.

• 대한한방소아과학회지
• /
• 제20권2호
• /
• pp.19-34
• /
• 2006

Objective : The purpose of this study is to find out the effect of Jowonsangamibang(JWS) on the growth of rats. Methods : First of all, we divided male Sprague-Dawley rats into 4 groups(J1, J2, J3 and sham group). Then J1, J2 and J3 groups were administered with JWS water extracts(JWSE) once a day at a dosage 250, 500, 1,000mg/kg respectively for 3 weeks. The sham group was administered with normal saline in the same manner. And we measured body weight, amount of body weight increased, length of femur, serum GH, serum IGF-I, serum TSH and serum testosterone after each week. Results : There were no significant changes of body weight, the level of GH, TSH, and testosterone in J1, J2 and J3 groups. The change of body weight gained was increased significantly in the J3 group compared with the sham group after 1, 2 and 3 weeks. The length of the femur was increased significantly in the J2 group after 1 week and in the J3 group after 3 weeks compared with the sham group. The level of IGF-I in the serum was increased significantly in J1, J3 after 1 week compared with the sham group. Conclusion : According to the results above, JWS increased body weight, length of femur, serum GH, serum IGF-1 compared with the sham group. This study shows that JWS has an effect on promoting the growth of rats, thus it's expected to treat of growth delay in children.

• 대한본초학회지
• /
• 제29권5호
• /
• pp.31-38
• /
• 2014

Objectives : Egg yolk is composed of various important chemical substances for human health. A calcium shortage causes the growth retardation on the body growth. In this study, we examined the therapeutic effects of calcium, vitamin D and egg yolk peptide (EYP) treatment on the retardation of the longitudinal bone growth induced by low-calcium diet in adolescent rats. Methods : Low calcium diets were administrated for 15 days. During the last five days, calcium and/or vitamin D and/or EYP were administrated. The body weights, longitudinal bone growth rates, the heights of growth plates, and bone morphogenetic protein (BMP)-2 and insulin-like growth factor (IGF)-1 expressions were measured using histochemical analysis. Results : Low calcium diets caused the significant reduction in body weight gains and the longitudinal bone growth. The heights of growth plates and the expressions of BMP-2 and IGF-1 showed the impairment of body growth as well. Calcium and/or vitamin D administration could not significantly increase the longitudinal bone growth. However, calcium, vitamin D, and EYP administration significantly increased the bone growth, the growth plate height, and BMP-2 and IGF-1 expressions. Conclusions : These results suggest that EYP enhances the longitudinal bone growth in the calcium and/or vitamin D deficiency and it could be a promising agent for the treatment of children suffering from malnutrition.

• 한국축산식품학회:학술대회논문집
• /
• 한국축산식품학회 2004년도 정기총회 및 제33차 춘계 학술대회
• /
• pp.314-317
• /
• 2004

IGF-I rich fraction 분리는 분만 후 24시간이내에 착유한 젖소 초유를 30kDa과 1kDa Ultrafiltra-tion(UF) membrane을 이용하여 분리하였다. 분리한 IGF-I rich fraction은 sandwich ELISA로 정량한 결과 1mg/ml 각 암세포에 미치는 영향을 실험하였다. 그 결과IGF-I rich fraction $1mg/m{\ell}$의 농도로 처리하였을 때 A-427ce11은 33%, SK-HEP-1 cell은 약 2%, A498 cell은 약 22%의 암세포 성장저해 효과를 보였고 HeLa는 약 5% 이하의 세포 증식 저해율과 WiDr cell은 약 30%의 세포성장 저해율을 나타내었으며, 유일하게 위암 세포주인 SNU 16에 대해서는 $1mg/m{\ell}$과 $100{\mu}g/m{\ell}$ 및 $10{\sim}1{\mu}g/m{\ell}$의 농도에서 약 15%와 10% 및 약 5% 이하의 세포 증식율을 나타내었다.

• Journal of Animal Science and Technology
• /
• 제55권2호
• /
• pp.95-101
• /
• 2013

The present study was conducted to test a hypothesis that pork quality traits would be influenced by the systemic and/or local bioavailability of insulin-like growth factor-I (IGF-I), transforming growth factor-${\beta}1$ (TGF-${\beta}1$), or epidermal growth factor (EGF) before or at slaughter. To this end, 60 cross-bred female market pigs weighing approximately 110 kg were slaughtered, after which Longissimus dorsi muscle (LM) samples taken at slaughter (D 0) and blood samples taken at D -7 and D 0 were analyzed. The 60 carcasses rendered 36 RFN (reddish-pink, firm, and non-exudative), 16 RSE (reddish-pink, soft, and exudative), and 6 PSE (pale, soft, and exudative); 2 DFD (dark, firm, and dry) also were found but were excluded in subsequent experiments. The $L^*$ and drip loss were greater in PSE vs. RFN and RSE and in PSE and RSE vs. RFN, respectively, as they should (P<0.05). The $pH_{45min}$ was less in PSE vs. RFN (P<0.05); $pH_{24h}$ tended to be less in the former (P=0.09). The LM IGF-I and TGF-${\beta}1$ as well as serum EGF concentrations were less in PSE than in RFN. None of the other LM and serum concentrations of the three growth factors differed across the three pork quality categories. The LM IGF-I and TGF-${\beta}1$ concentrations and serum EGF concentration at D 0 were negatively correlated with drip loss [r = -0.36(P<0.01), -0.44 (P<0.01), and -0.32 (P<0.05), respectively]. However, none of the serum and LM growth factor variables was correlated with $L^*$ or $a^*$ (redness) of LM. Taken together, results suggest that locally expressed IGF-I and TGF-${\beta}1$ and blood-borne EGF may have a beneficial effect on postmortem water holding capacity of the muscle and that pork quality traits could be predicted to some extent from concentrations of IGF-I and TGF-${\beta}1$ in muscle and EGF in serum at slaughter.

• 한국산학기술학회논문지
• /
• 제12권4호
• /
• pp.1756-1762
• /
• 2011

이 연구 목적은 기수를 대상으로 공복 시 반복적인 모의 경주마 운동 시 그렐린과 관련 호르몬의 관계를 규명하고자 한다. 공복의 기수와 기수후보생들은 경마의 첫 번째 단거리 1000 m, 두 번째 중거리 1700 m 세 번째 장거리 2300 m의 반복적인 모의 경주마 운동 전과 직후 정맥혈을 채혈하여 그렐린, 렙틴, 인슐린, IGF-I과 코티졸을 분석하였다. 피어슨 상관관계에서 기수후보생이 3차 운동 후 글레린과 인슐린 농도가 r= .55를 나타내었다. 기수의 운동 전 글레린과 렙틴 농도가 r= .73으로 운동전 글레린 렙틴 수준이 통계적으로 유의성(p<.05) 있는 관계와 기수후보생이 글레린과 코티졸 농도에서 r= .64를 나타내었다. 글레린 렙틴 인슐린 IGF-1 농도는 운동전에 비교하여 통계적으로 유의성 없이 1차 운동 후 증가와 2차, 3차 감소, 경마기수와 후보생보도 통계적으로 유의성 없는 차이를 나타내었다. 경마기수에 비교 하여 후보생은 1차 2차, 3차 운동에서 통계적으로 유의성 있게 IGF-1는 낮게(p<.05) 코티졸 농도는 높게(p<.001) 나타내었다. 연구결과 반복적인 모의 경주마 운동으로 인한 에너지 소모 반응에서 기수후보생의 3차 운동에서 만이 글레린과 인슐린의 상관관계를 나타내었다.

• Asian-Australasian Journal of Animal Sciences
• /
• 제23권3호
• /
• pp.379-384
• /
• 2010

The principal objective of this experiment was to determine the effects of dietary betaine on IGF-I, IGFBP-3 and IGFBP-1 secretion and IGF-I mRNA gene expression in the serum and liver of laying hens. A total of 72 ISA-Brown laying hens were fed with four different levels of betaine (0, 300, 600, 1,200 ppm) based on a corn-soybean meal diet containing 2,800 kcal/kg of metabolizable energy (ME) and 16% crude protein (CP) for four weeks. The results indicated significantly higher serum and liver IGF-I concentrations in the laying hens fed with 600 and 1,200 ppm betaine (p<0.05) compared to controls. IGF-I gene expression in liver showed a statistically correlated increase in 600 and 1,200 ppm betaine-fed groups as compared to the controls (p<0.05). Serum IGFBP-3 concentrations were elevated significantly in the groups fed 600 ppm of betaine. However, the secretion of IGFBP-1 in the liver of laying hens fed on 600 and 1,200 ppm of betaine was significantly lower than in the controls (p<0.05). The results of this experiment showed that dietary betaine supplementation plays a pivotal role in changes of the IGFs system in laying hens.

• 셀메드
• /
• 제4권2호
• /
• pp.14.1-14.6
• /
• 2014

KH-BaRoKer-SeongJangTang (KBS) is a recently developed formulation by using traditional drugs considering traditional medical theory of Oriental books such as ShinNongBonChoGyeong and JuRye, which has been used to improve the growth of child in Korea. Although KBS is usually prescribed to many children who are in retard for their age, its pharmacological effects have not been fully understood in experimental models. The aim of this study was to evaluate the effects of KBS on bone growth. Growth plate thickness and bone parameters such as bone volume/tissue volume (BV/TV), trabecular thickness (Tb.Th), trabecular number (Tb.N), connection density (Conn.D), and total porosity were analyzed by means of microcomputed tomography. Serum insulin-like growth factor-I (IGF-I) levels were measured by enzyme-linked immunosorbent assay. Hepatic IGF-I mRNA expression was analyzed by real-time polymerase chain reaction. Phosphorylation of signal transducer and activator of transcription5 (STAT5) was investigated using Western blot analysis and immunohistochemistry. The thickness of growth plate was increased by KBS. BV/TV, Tb.Th, TbN, Conn.D, and total porosity were improved by KBS. Hepatic IGF-I mRNA and serum IGF-I levels were elevated by KBS. Phosphorylation of STAT5 was increased with administration of KBS. These results suggest that KBS would be helpful to children who are in retard for their age through the elevation of IGF-I.