• The Korean Journal of Food And Nutrition
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• v.23 no.4
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• pp.526-530
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• 2010

Isopentenyl diphosphate(IPP) isomerization to dimethylallyl diphosphate(DMAPP) is an important step for the efficient production of isoprenoids such as lycopene, ${\beta}$-carotene, astaxanthin, etc. The type II isopentenyl diphosphate isomerase gene from Synechocystis sp. PCC6803(sll1556, Syidi2) was cloned and expressed in Escherichia coli $DH5{\alpha}$. When E. coli $DH5{\alpha}$ harboring lycopene synthesis genes, crtE, crtB, and crtI and mevalonate pathway genes, MvK1, MvK2, and Mvd, was cultured on LB medium containing mevalonate, the strain grew very slowly be due to the toxicity of isopentenyl diphosphate derived from mevalonate. When Syidi2 was introduced to E. coli $DH5{\alpha}$ harboring the lycopene synthesis genes and mevalonate pathway genes, growth on mevalonate medium was fully restored and the colony showed red color indicating lycopene formation. The growth rate of the mutant strain, E. coli $DH5{\alpha}$(idi::${\Delta}km$), was very slow because of IPP accumulation and DMAPP deprivation. Ultimately the idi mutant was complemented by introducing the Syidi2 gene.

• Journal of Life Science
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• v.24 no.7
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• pp.762-768
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• 2014

Diet exerts a major stress on the body and may affect gene expression and physiological functions. Understanding of cellular responses during starvation is necessary in developing strategies to reduce damage caused by diet. In this study, we isolated 10 genes (Comt, RGN, Scd1, Temt, Idi1, Fabp5, Car3, Cyp2c70, Pinx1, and Poldip3) that are down-regulated in starvation and are closely related to liver metabolism. Water supply during starvation had no effect on the induction of apoptosis, autophagy, and ERQC. The genes down-regulated by starvation were associated with many related pathways rather than limited to the liver homeostasis pathway. Water supply during starvation is important. However, maintaining NaCl homeostasis is more important. The results are thought to be closely related to gender-specific metabolism in starvation and NaCl.

• Mycobiology
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• v.51 no.3
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• pp.169-177
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• 2023

To further explore the molecular mechanism of triterpenoid biosynthesis and acquire high-value strain of Sanghuangporus baumii, the Agrobacterium tumefaciens-mediated transformation (ATMT) system was studied. The key triterpenoid biosynthesis-associated gene isopentenyl diphosphate isomerase (IDI) was transformed into S. baumii by ATMT system. Then, the qRT-PCR technique was used to analyze gene transcript level, and the widely targeted metabolomics was used to investigate individual triterpenoid content. Total triterpenoid content and anti-oxidant activity were determined by spectrophotometer. In this study, we for the first time established an efficient ATMT system and transferred the IDI gene into S. baumii. Relative to the wild-type (WT) strain, the IDI-transformant (IT) strain showed significantly higher transcript levels of IDI and total triterpenoid content. We then investigated individual triterpenoids in S. baumii, which led to the identification of 10 distinct triterpenoids. The contents of individual triterpenoids produced by the IT2 strain were 1.76-10.03 times higher than those produced by the WT strain. The triterpenoid production showed a significant positive correlation with the IDI gene expression. Besides, IT2 strain showed better anti-oxidant activity. The findings provide valuable information about the biosynthetic pathway of triterpenoids and provide a strategy for cultivating high-value S. baumii strains.

• Journal of the Korea Society for Simulation
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• v.20 no.2
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• pp.1-10
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• 2011

In queueing network analysis, arrival processes are usually modeled as renewal processes by matching mean and variance. The renewal approximation simplifies the analysis and provides reasonably good estimate for the performance measures of the queueing systems under moderate conditions. However, high variability in arrival process or in service process requires more sophisticated approximation procedures for the variability parameter of departure/arrival processes. In this paper, we propose an heuristic approach to refine Whitt's variability function with the k-interval squared coefficient of variation also known as the index of dispersion for intervals(IDI). Regression analysis is used to establish an empirical relationships between the IDI of arrival process and the IDI of departure process of a queueing system.

• International Journal of Automotive Technology
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• v.8 no.2
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• pp.179-184
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• 2007

In this study, the potential use of oxygenated fuels such as ethylene glycol mono-normal butyl ether (EGBE) was investigated in an attempt to reduce the emission of exhaust smoke from diesel engines. Effects of the combustion method on exhaust emission of DI and IDI diesel engines were also examined. Since EGBE is composed of approximately 27.1% oxygen, this is one of several potential oxygenated fuels that could reduce the smoke content of exhaust gas. EGBE blended fuels have been proven to reduce smoke emission remarkably compared to the conventional commercial fuels. The test was conducted with single and four cylinder, four stroke, DI and IDI diesel engines. The study showed that a simultaneous reduction of smoke and NOx emission could be achieved by the combination of oxygenated blend fuels and the cooled EGR method in both DI and IDI diesel engines. It was also found that a reduction rate of exhaust emission in a DI engine was larger than an IDI diesel engine.

• BMB Reports
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• v.50 no.9
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• pp.466-471
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• 2017

The results of this study show that c-Jun N-terminal kinase (JNK) activation was associated with the enhancement of docetaxel-induced cytotoxicity by simvastatin in DU145 human prostate cancer cells. To better understand the basic molecular mechanisms, we investigated simvastatin-regulated targets during simvastatin-induced cell death in DU145 cells using two-dimensional (2D) proteomic analysis. Thus, vimentin, Ras-related protein Rab-1B (RAB1B), cytoplasmic hydroxymethylglutaryl-CoA synthase (cHMGCS), thioredoxin domain-containing protein 5 (TXNDC5), heterogeneous nuclear ribonucleoprotein K (hnRNP K), N-myc downstream-regulated gene 1 (NDRG1), and isopentenyl-diphosphate Delta-isomerase 1 (IDI1) protein spots were identified as simvastatin-regulated targets involved in DU145 cell death signaling pathways. Moreover, the JNK inhibitor SP600125 significantly inhibited the upregulation of NDRG1 and IDI protein levels by combination treatment of docetaxel and simvastatin. These results suggest that NDRG1 and IDI could at least play an important role in DU145 cell death signaling as simvastatinregulated targets associated with JNK activation.

• Korean Journal of Veterinary Research
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• v.34 no.3
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• pp.529-536
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• 1994

To establish more specific and simple diagnostic methods for detection of the antibodies and antigens of Aujeszky's disease virus(ADV), we designed indirect dot-immunoassay(IDI) and double sandwich dotimmunoassay(DSDI) using the solid phases of nitrocellolose paper and polystyrene plate. The diagnostic efficacy of these methods was investigated. As the sensitivity of IDI was tested by various virus concentration, the specimens with the virus titer above $10^{4.0}TCID_{50}/0.2ml$ showed positive reaction, but that below $10^{1.0}TCID_{50}/ml$ revealed negative. Tonsil emulsion at the virus titer of $10^{4.5}TCID_{50}/0.2ml$ showed the highest sensitivity as diluted by 1/100. In detection of ADV antigens from the various tissues of the rats and pigs infected with ADV, IDI using monoclonal antibody showed the higher specificity as compared with IDI using polyclonal antibody and virus isolation method. The efficacy of the DSDI for detection of ADV antibody was compared with other tests. The sensitivity of DSDI was higher than virus neutralization(VN) and agar gel immunodiffusion test(AGID). Meanwhile, specificity of DSDI was lower than AGID, but similar to IDEA. In comparison with VN test, DSDI showed 96.9% agreement to VN test that is the highest of three tests. In general, application of polyclonal antibody in both tests caused the higher sensitivity but the lower specificty.

• Toxicological Research
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• v.35 no.1
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• pp.13-24
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• 2019

Numerous ethnomedicinal uses have been attributed to different parts of Annona senegalensis (ASE), including its uses as food and food additives. The present study investigated toxicological and antioxidant effects of 28 days administration of ethanol extracts of ASE stem bark to Wistar strain albino rats. Acute toxicity test was done to determine lethal dose in Wistar rats while sub-acute toxicity test was conducted on rats divided into four groups (A - control, B - 50 mg/kg, C - 100 mg/kg, D - 150 mg/kg, respectively and treated for 28 days. Oxidative stress markers in liver and kidney as well as hepatic succinate dehydrogenase activity in the mitochondrial and post mitochondrial fractions (PMF) were evaluated. The $LD_{50}$ value of ASE was > 2,000 mg/kg. White blood cell counts gradually increased, but red blood cell counts and haematocrits level decreased significantly (p < 0.05) by about 50%. Liver enzymes in the serum and mitochondrial succinate dehydrogenase activity increased significantly (p < 0.05). Superoxide dismutase and catalase activities also increased in liver mitochondria and PMF while malondialdehyde (MDA) and reduced glutathione levels increased only in the PMF. Furthermore, only MDA levels increased significantly in the kidney after 28 days extract administration. Histopathological examination showed hepatic necrosis and no obvious signs of nephrotoxicity. Anona senegalensis is relatively safe, but prolonged ingestion could induce oxidative stress and impair ATP synthesis through the modulation of the activity of mitochondrial succinate dehydrogenase.

• Transactions of the Korean Society of Automotive Engineers
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• v.14 no.4
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• pp.115-122
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• 2006

To evaluate the durability characteristics of in-direct injection diesel engine using BDF 20(a blend of 20% biodiesel fuel and 80% diesel fuel in volume), an IDI diesel engine used to commercial vehicle was operated on BDF 20 for 300 hours. Engine dynamometer testing was completed at regularly scheduled intervals to investigate the combustion characteristics, engine performance and exhaust emissions. The engine performance and exhaust emissions were sampled at 1 hour interval for analysis. From the results, the combustion variations such as the combustion maximum pressure($P_{max}$) and the crank angle at which this maximum pressure occurs(${\Theta}_{Pmax}$) were not appeared during long-time dynamometer testing. Also, BSFC with BDF 20 resulted in lower than with diesel fuel. The peak pressure with BDF 20 was higher than that with diesel fuel due to the oxygen content in BDF. And, BDF 20 resulted in lower emissions of carbon monoxide, carbon dioxide, and smoke emissions with a little increase of oxides of nitrogen than diesel fuel. It was concluded that there was no unusual deterioration of the engine, or any unusual change in exhaust emissions during the durability test of an IDI diesel engine using BDF 20.

• Transactions of the Korean Society of Automotive Engineers
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• v.11 no.1
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• pp.72-78
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• 2003

Recently, lots of researchers have been attracted to develope various alternative fuels and to use renewable fuels as a solution of environmental problems from automobile. The use of biodiesel fuel is an effective way of substituting diesel fuel in the long nun. It is a domestically produced, renewable fuel that can be manufactured from vegetable oils, used vegetable oils, or animal fats. In this study, the usability of biodiesel fuel derived from rice ban oil, one of the oxygenated fuels as an alternative fuel for diesel engines was investigated in IDI diesel engine. Emissions were characterized with neat biodiesel fuel and with a blend of biodiesel fuel and conventional diesel fuel. Since the biodiesel fuel includes oxygen of about 11%, it could influence the combustion process strongly. So, the use of biodiesel fuel resulted in lower emissions of carbon monoxide and smoke emissions with some increase in emissions of oxides of nitrogen. It is concluded that biodiesel fuel can be utilized effectively as a renewable fuel for IDI diesel engine.