• Applied Biological Chemistry
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• v.31 no.3
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• pp.274-283
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• 1988

Aqualysin I is an alkaline serine protease which is secretet into the culture medium by Thermus aquaticus YT-1, an extreme thermophile. Aqualysin I was purified, and its partial amino acid sequence was determined. The gene encoding aqualysin I was cloned into E. coli using synthetic oligodeoxyribonucleotides as hybridization probes. The nucleotide sequence of the cloned DNA was determined. The primary structure of aqualysin I, deduced from the nucleotide sequenc, agreed with the determid amino acid sequences, including the $NH_2-$ and COOH terminal sequence of the tryptides derived from aqualysin I. Aqualysin I comprised 281 amino acid residues and its molecular mass was determined to be 28350. On alignment of the whole amino acid sequence, aqualysin I showed high sequence homology with the subtilisin type serine protease, and 43% identity with proteinase K, 37-30% with subtilisins and 34% with thermitase. Extremely high sequence identity was observed in the regions containing the active-site residues, corresponding to Asp32, His64 and Ser221 of subtilisin BPN'. Aqualysin I contains two disulfide bonds, Cys67-Cys99 and Cys163-Cys194, and these disulfide bonds seem to contribute to the heat stability of the enzyme. The determined positions of the twe disulfide bonds of aqualysin I agreed with those predicted previously on the basis of computer graphics of the crystallographic data for subtilisin BPN'. Therefore, these findings sugests that the three-dimensional structure of aqualysin I is similar to that of subtilisin BPN' Aqualysin I is produced as a lage precursor, which contains $NH_2-$ and COOH- terminal portions besides the mature protease sequence.

• Journal of Digital Convergence
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• v.16 no.7
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• pp.145-151
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• 2018

This paper is a study on city brand development which is increasingly interested as a means to enhance the attractiveness of the city. the previous research, the design of city brand identity was an application from the internal stakeholder's point of view related to the metropolitan government. However, in the present study, we tried to take a external approach that perceptions and designs city brands from the related to the consumer. For the purpose of research, based on previous studies, we extracted three images of leading, stable and amusing city brand images and prepared three scenarios for the imaginary city as experimental stimuli. The results showed that the overall city brand attitude, residence intention, and tourism intention to the imaginary city were significantly different according to the leading, stability and amusement brand image. According to the results of this study, it is necessary to continuously effort the development of city brand image which is perceived by consumers in order to differentiate city.

• Journal of Korean Home Economics Education Association
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• v.24 no.1
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• pp.85-104
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• 2012

The purpose of this study was to carry out the career portfolio and the teacher-directed career exploration program in the unit of 'Industry and Career' of Technology·Home Economics and to find out how much those programs affect on career maturity and career identity in middle school students. The subjects were third-grade students who were selected at random from a middle school in Daegu. The subjects were divided into 60 for career portfolio group, 60 for teacher-directed group, and 60 for control group. Those programs were conducted totally in 9 sessions once or twice a week, and each session took 45 minutes. For pretest and post test, the questionnaire consisting of career maturity scale and career identity scale was used. To analyze data, ANCOVA was used and Scheffe test was conducted to examine significant differences between three groups. The main result of this study was the following : First, students who participate in the career portfolio and the teacher-directed career exploration program are more improved than those who have not been taught anything in determinacy and purpose of career maturity. Also, students who join in the career portfolio career exploration program are much more improved than those who join in the teacher-directed career exploration program in confidence and preparedness of career maturity. Second, students who join in the career portfolio career exploration program are more improved than those who join in the teacher-directed career exploration program in stability, goal directivity and assertiveness of career identity. Also students who participate in the lecture-oriented career exploration program are more improved meaningfully than those who have not been taught anything in the task orientation of career identity.

• The Korean Journal of Quaternary Research
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• v.17 no.2
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• pp.85-99
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• 2003

Since the experience of major landscape change during last half of the century due to war and rapid urbanization, the traditional character has been weakened, and it is necessary for a reconsideration to improve the landscape for the future. To review these relationships, the importance of a comprehensive understanding of nature has been suggested. Therefore identification of a new concept based on the 'socio-cultural influence of landscape' and 'sense of place' which are related to peoples' previous experience, is required. Furthermore more practical definitions and criteria to reveal the relationship are necessary. Among the terms suggested to describe sense of place such as 'home', 'place identity', 'place-based meaning' and 'settlement identity' etc., the 'home' is selected to represent our surrounding landscape. For more practical classification of home landscape, additional terms are suggested and defined based on both the relationships between human beings and nature, and between memory derived from previous experience and shared values with in the community. The additional terms which are the most important in the role of landscape character related to humans' are; ⅰ) Personal Landscape: Landscape of an individual human, which derives from previous personal experience; involves distinguishable character for a given person, and it is emotional and flexible depending on circumstances. ⅱ) Ordinary Landscape： Landscape of the 'common interest' between members of a community, which is acceptable as a surrounding for everyday daily life, it produces the richness and variety of landscape. ⅲ) Kernel Landscape： Landscape of the 'common ground' which is acceptable to the majority members of the community, and it provides variety and stability for periods of time, and it could strongly represent community attitudes toward nature. ⅳ) Prototype Landscape: Landscape as the 'common denominator' of overall community from past to present and towards the future, which encompasses all the kernel landscape throughout history. It provides a sense of place, balances the homogeneity of character throughout overall communities. Some part of this can be shared throughout history to shape an overall sense of place. It can also represent short terms fashions. For a prototype landscape to reveal sense of place, there are a couple of points which we should underline the commencing point. Firstly, understanding the relationship between humans and nature should be based on a given character of surroundings. Secondly, reoccurring landscape elements which have sustained in history can lead to sense of place, and should be reviewed the influences between nature and humans.

• Journal of Microbiology and Biotechnology
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• v.24 no.2
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• pp.197-208
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• 2014

A novel protease gene from Bacillus gibsonii, aprBG, was cloned, expressed in B. subtilis, and characterized. High-level expression of aprBG was achieved in the recombinant strain when a junction was present between the promoter and the target gene. The purified recombinant enzyme exhibited similar N-terminal sequences and catalytic properties to the native enzyme, including high affinity and hydrolytic efficiency toward various substrates and a superior performance when exposed to various metal ions, surfactants, oxidants, and commercial detergents. AprBG was remarkably stable in 50% organic solvents and retained 100% activity and stability in 0-4 M NaCl, which is better than the characteristics of previously reported proteases. AprBG was most closely related to the high-alkaline proteases of the subtilisin family with a 57-68% identity. The secretion and maturation mechanism of AprBG was dependent on the enzyme activity, as analyzed by site-directed mutagenesis. Thus, when taken together, the results revealed that the halo-solvent-tolerant protease AprBG displays significant activity and stability under various extreme conditions, indicating its potential for use in many biotechnology applications.

• The Journal of the Korea Contents Association
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• v.21 no.3
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• pp.390-399
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• 2021

This study analyze the factors that can improve the satisfaction level of college students stable adaptation to college life and education service. It was analyzed whether students' personal characteristics, college life-related characteristics, and changes in college life Adaptation increase the satisfaction of college education service. As a result of analyzing CAT(college adatiation test) differences of 1st survey and 2nd survey in the results of the total 368 students, the graduates of the academic high school showed high academic integration and social integration and the social integration was high in the types of regular admission. As a result of multiple regression analysis with the increase in college education service satisfaction as a dependent variable, the lower the language score, the more positive changes in academic integrity, career identity, college satisfaction, relationship with professors, economic stability, and study keeping stability. Based on these results, various student support programs were proposed to increase the college education service satisfaction of college students.

• Proceedings of the KSRS Conference
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• 2005.10a
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• pp.678-682
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• 2005

IETF suggested AAA for safe and reliable user authentication on various network and protocol caused by development in internet and increase in users. Diameter standard authentication system does not provide mutual authentication and non-repudiation. AAA authentication system using public key was suggested to supplement such Diameter authentication but application in mobile service control nodes is difficult due to overhead of communication and arithmetic. ID based AAA authentication system was suggested to overcome such weak point but it still has the weak point against collusion attack or forgery attack. In this thesis, new ID based AAA authentication system is suggested which is safe against collusion attack and forgery attack and reduces arithmetic quantity of mobile nodes with insufficient arithmetic and power performance. In this thesis, cryptological safety and arithmetical efficiency is tested to test the suggested system through comparison and assessment of current systems. Suggested system uses two random numbers to provide stability at authentication of mobile nodes. Also, in terms of power, it provides the advantage of seamless service by reducing authentication executing time by the performance of server through improving efficiency with reduced arithmetic at nodes.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.19 no.7
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• pp.222-230
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• 2018

This paper examines gender trouble in the future society from Huxley's Brave New World, in which science is highly developed. In addition, I examined how gender equality was achieved in the World State in which equality and identity were emphasized, and considered the precise meaning of gender equality. Superficially, there are many gender inequality factors in the Reservation, and gender equality is achieved in the World State. However, if we look closely at the World State, we realize that they deprive individual rights under the name of social stability. Therefore, we should reconsider what real gender equality is, and we need to examine what is needed to achieve gender equality.

• Elastomers and Composites
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• v.42 no.2
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• pp.112-118
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• 2007

An HRP immobilized carbon paste electrode, which was bound by EPDM, was newly fabricated and its electrochemical properties were investigated for the purpose of validating the new possibility for the practical use of biosensor. In the experimental range of substrate concentration, Lineweaver-Burk plot of the signal showed a good linearity. This means that HRP was embedded effectively to preserve its identity in the bulk of composite electrode materials and EPDM is a recommendatory binder. When the electrode was run at low operating potential($0.0\sim-1.0$ V vs. Ag/AgCl), it showed a high sensitivity and a good reproducibility. Especially the mechanical stability of the dried rubber was a remarkable breakthrough to get over a difficulty in putting the carbon-paste electrode bound with silicon oil to real use.

• Proceedings of the Korea Concrete Institute Conference
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• 2008.11a
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• pp.485-488
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• 2008

Fire intensity in tunnel fire is very severe, which might cause the spalling on the surface of shotcrete and concrete lining exposed to the heat as well as rapidly-reducing stress due to heat transfer by steel material such as anchor embedded in tunnel which plays the critical role in securing the stability of the tunnel. In this study, a fire test to identity the heat intensity(Modifired Hydrocarbon Curve) and the fire resistance of steel materials embedded as parameters, was carried out. And the evaluation to identify the thermal damage, which was based on critical temperature range for thermal damage of steel materials determined according to the road tunnel fire resistance standard established by ITA(International Tunneling Association).