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Electrochemical Properties of HRP Immobilized Biosensor bound with EPDM  

Yoon, Kil-Joong (Division of Applied Sciences, Cheongju University)
Publication Information
Elastomers and Composites / v.42, no.2, 2007 , pp. 112-118 More about this Journal
Abstract
An HRP immobilized carbon paste electrode, which was bound by EPDM, was newly fabricated and its electrochemical properties were investigated for the purpose of validating the new possibility for the practical use of biosensor. In the experimental range of substrate concentration, Lineweaver-Burk plot of the signal showed a good linearity. This means that HRP was embedded effectively to preserve its identity in the bulk of composite electrode materials and EPDM is a recommendatory binder. When the electrode was run at low operating potential($0.0\sim-1.0$ V vs. Ag/AgCl), it showed a high sensitivity and a good reproducibility. Especially the mechanical stability of the dried rubber was a remarkable breakthrough to get over a difficulty in putting the carbon-paste electrode bound with silicon oil to real use.
Keywords
Biosensor; Enzyme electrode; Ethylene-propylene rubber; Hydrogen peroxide; Peroxidase;
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Times Cited By KSCI : 4  (Citation Analysis)
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1 V. C. Sanz, M. L. Mena, A. G. Cortes, P. Yanez-Sedeno and J. M. Pingarron, 'Development of a Tyrosinase biosensor based on Gold nanoparticlesmodified Glassy carbon electrodes Application to the Measurement of a Bioelectrochemical polyphenols Index in Wines', Anal. Chim. Acta, 528, 1 (2005)   DOI   ScienceOn
2 P. Mailley, E. A. Cummings, S. Mailley, S. Cosnier, B. R. Eggins and E. McAdams, 'Amperometric detection of Phenolic compounds by Polypyrrole-based composite Carbon paste Electrodes', Biochemistry, 63, 291 (2004)
3 A. N. Diaz, M. C. R. Peinado and M. C. T. Minguez, 'Sol-gel Horseradish Peroxidase Biosensor for Hydrogen peroxide Detection by Chemiluminescence', Anal. Chim. Acta, 363, 221 (1998)   DOI   ScienceOn
4 K. J. Yoon, 'Optimum pH of the Reduction of Hydrogen peroxide at a Tobacco plant Tissue based Amperometric Biosensor', J. Kor. Chem. Soc. 48(6), 654 (2004)   DOI
5 J. A. Brydson, 'Rubbery Materials and Their Compounds', p. 147, Elsevier Applied Science, London and New York, 1988
6 B. Wang and S. Dong, 'Sol-gel-derived Amperometric Biosensor for Hydrogen peroxide based on Methylene green Incorporated in Nafion film', J. Electroanal. Chem. 487, 45 (2000)   DOI   ScienceOn
7 Y. Miao and S. N. Tan, 'Amperometric Hydrogen peroxide Biosensor with Silica sol-gel/chitosan Film as Immobilization Matrix', Anal. Chim. Acta, 437, 87 (2001)   DOI   ScienceOn
8 J. Wang, N. Naser, H. S. Kwon, and M. Y. Cho, 'Tissue bioelectrode for Organic-phase Enzymatic Assays', Anal. Chim. Acta, 264, 7 (1992)   DOI   ScienceOn
9 I. Vostiar, J. Tkac, E. Sturdik and P. Gemeiner, 'Amperometric urea biosensor based on Urea and Electropolimerized Toludine blue dye as a pH-sensitive Redox probe', Biochemistry, 56, 113 (2002)
10 H. S. Kwon, E. H. Jin, K. J. Yoon and Y. N. Pak, 'Mushroom-juice based Gold Electrode for the Determination of Phenols', J. Kor. Chem. Soc. 49(2), 224 (2005)   DOI
11 K. J. Yoon, 'A New Strategy for Determining Optimum pH of Isozymes', Bull. Kor. Chem. Soc. 25(7), 997 (2004)   DOI
12 A. N. Reshetilov, M. V. Donova, D. V. Dovbnya and A. M. Boronin, 'FET-microbial Sensor for Xylose detection based on Gluconobacter oxydans Cells', Biosensors & Bioelectronics, 11, 401 (1996)   DOI   ScienceOn
13 K. J. Yoon, 'Electrochemical Determination of the Optimum pH of HRP', Anal. Sci. Tech. 16(6), 504 (2003)
14 K. J. Yoon, K. J. Kim, and H. S. Kwon, 'Electrochemical Properties of the Chicken Small Intestinal Tissue based Enzyme Electrode for the Determination of Hydrogen Peroxide', J. Kor. Chem. Soc. 43(3), 271 (1999)
15 R. D. Schmid and F. Scheller, 'Biosensors Applications in Medicine, Environmental Protection and Process Control', Vol. 13, p. 4. Weinheim Germany, 1989
16 K. J. Yoon, H. S. Kwon, and B. G. Lee, 'Electrochemical Determination of pH-Stability Curve of Enzyme', J. Kor. Chem. Soc. 49(3), 325 (2005)   DOI
17 R. P. Buck, W. E. Hatfield, M. Umana, and E. F. Bowden, 'Biosensor Technology', p. 3, Marcel Dekker, Inc. U.S.A. 1990
18 E. Crouch, D. C. Cowell, S. Hoskins, R. W. Pittson and J. P. Hart, 'Amperometric, Screen-printed, Glucose biosensor for Analysis of human plasma samples using a Biocomposite water-based Carbon ink incorporating Glucose oxidase', Anal. Biochem. 347, 17 (2005)   DOI   ScienceOn