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Antioxidant and Antimutagenic Activity of Solvent-Fractionated Layers of Colored Rice Bran (유색미 품종별 유기용매 추출물의 항산화성 및 항변이원성 검정)

  • Kang, Mi-Young;Shin, Soo-Young;Nam, Seok-Hyun
    • Korean Journal of Food Science and Technology
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    • v.35 no.5
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    • pp.951-958
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    • 2003
  • Twenty-four cultivars of colored rice seeds were collected inside and outside Korea, and the antioxidant and antimutagenic activity was determined for the solvent-fractionated layers of their bran parts lipid soluble fraction, pigment containing fraction, and pigment component per se. As the serial organic solvent extraction proceeded, the overall tendency of antioxidant activities declined with increased chemical homogeneity of each fraction. This markedly showed the low antioxidativities of the pigment components from LK 1-3-6-12-1-1 and Gillimhukmi. Even all the colored rice cultivars, with considerable antimutagenic activity in 70% ethanolic extract, exhibited mutagenicity when measured with its pigment containing fraction (wx 124-163-45-7-1-1-1 and LKlB-2-1-1 being the strongest). The pigment content in each colored rice seeds decreased in the order of IR 17491-5-4-3-3>LK 1-3-6-12-1-1>LK 1D-2-12-1>RGS No.336, Elwee. In addition, a substantial difference in both chemical composition of the constituents and its amount could be found between the colored rice and cooking rice cultivars. This revealed that, compared to cooking rice, major components of organic solvent fractions from colored rice probably have long hydrocarbon chain moieties.

Molecular Cloning and Expression of a Cellulolytic Xylanase Gene from Bacillus circulans in Escherichia coli (Bacillus circulans 기원의 Cellulolytic Xylanase 유전자의 대장균에서의 클로닝 및 발현)

  • 이동석;김지연;김한복
    • Korean Journal of Microbiology
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    • v.36 no.3
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    • pp.196-202
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    • 2000
  • A gene for cellulolytic xylanase of Bacillus circulnns ATCC21365 was cloned on pUC 19 in Eschwichia coli. The recombinant plasniid pXLI80 contained an 1.8 id, inselt composed of0.5 kb and 1.3 kb PslI fragments derived from B, circulans. The 0.5 kh fragment in the upstream region of 1.3 kb one was confirmed lo be indispensable for not only expression but also hyperexpression of the cloned gene. The transformant overproduced the xylanase 135 times greater than that produced by the orlginal B circulnns. The optimum pH and temperature of the cloned enzyme we]-e pH 5.2 and $60^{\circ}C$, respectively. Heal pretl-eatment at TEX>$55^{\circ}C$C for 1 Indid not cause inhibition of the activity of this enzyme. The elm.ynie could hydl-olyre CMC and lichenan as well as xylan to produce xylose(or GI), xylohiose(or G2) and xylolnose(or G3) as inah products. Hence We defined the cloned enzyme as a cellulolytic xylanase. The SDS-PAG electrophoretic mobility and zyiiogram of this enzyme derived from whole cell extracts or c~~lture supematants or E. coli(pXL180) indicated a molecular weight of 45,000 and nonprocessing of the enzyme in the peilplasln of E. coli.

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A Study on Features Analysis for Retrieving Image Containing Personal Information on the Web (인터넷상에서 개인식별정보가 포함된 영상 검색을 위한 특징정보 분석에 관한 연구)

  • Kim, Jong-Bae
    • Journal of the Institute of Electronics Engineers of Korea CI
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    • v.48 no.3
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    • pp.91-101
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    • 2011
  • Internet is becoming increasingly popular due to the rapid development of information and communication technology. There has been a convenient social activities such as the mutual exchange of information, e-commerce, internet banking, etc. through cyberspace on a computer. However, by using the convenience of the internet, the personal IDs(identity card, driving license, passport, student ID, etc.) represented by the electronic media are exposed on the internet frequently. Therefore, this study propose a feature extraction method to analyze the characteristics of image files containing personal information and a image retrieval method to find the images using the extracted features. The proposed method selects the feature information from color, texture, and shape of the images, and the images as searched by similarity analysis between feature information. The result which it experiments from the image which it acquires from the web-based image DB and correct image retrieval rate is 89%, the computing time per frame is 0.17 seconds. The proposed method can be efficiently apply a system to search the image files containing personal information and to determine the criteria of exposure of personal information.

Extinction Limits of Low Strain Rate Counterflow Nonpremixed Flames in Normal Gravity (정상 중력장에서 낮은 스트레인율을 갖는 대향류 비예혼합화염의 소화한계)

  • Oh, Chang-Bo;Choi, Byung-Il;Kim, Jeong-Soo;Hamins, Anthony;Park, Jeong
    • Transactions of the Korean Society of Mechanical Engineers B
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    • v.29 no.9 s.240
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    • pp.997-1005
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    • 2005
  • The extinction characteristics of low strain rate normal gravity (1-g) nonpremixed methane-air flames were studied numerically and experimentally. A time-dependent axisymmetric two-dimensional (2D) model considering buoyancy effects and radiative heat transfer was developed to capture the structure and extinction limits of 1-g flames. One-dimensional (1D) computations were also conducted to provide information on 0-g flames. A 3-step global reaction mechanism was used in both the 1D and 2D computations to predict the measured extinction limit and flame temperature. A specific maximum heat release rate was introduced to quantify the local flame strength and to elucidate the extinction mechanism. Overall fractional contribution by each term in the energy equation to the heat release was evaluated to investigate the multi-dimensional structure and radiative extinction of 1-g flames. Images of flames were taken for comparison with the model calculation undergoing extinction. The two-dimensional numerical model was validated by comparing flame temperature profiles and extinction limits with experiments and ID computation results. The 2D computations yielded insight into the extinction mode and flame structure of 1-g flames. Two combustion regimes depending on the extinction mode were identified. Lateral heat loss effects and multi-dimensional flame structure were also found. At low strain rates of 1-g flame ('Regime A'), the flame is extinguished from the weak outer flame edge, which is attributed to multi-dimensional flame structure and flow field. At high strain rates, ('Regime B'), the flame extinction initiates near the flame centerline due to an increased diluent concentration in reaction zone, which is the same as the extinction mode of 1D flame. These two extinction modes could be clearly explained with the specific maximum heat release rate.

Energy-Aware Self-Stabilizing Distributed Clustering Protocol for Ad Hoc Networks: the case of WSNs

  • Ba, Mandicou;Flauzac, Olivier;Haggar, Bachar Salim;Makhloufi, Rafik;Nolot, Florent;Niang, Ibrahima
    • KSII Transactions on Internet and Information Systems (TIIS)
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    • v.7 no.11
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    • pp.2577-2596
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    • 2013
  • In this paper, we present an Energy-Aware Self-Stabilizing Distributed Clustering protocol based on message-passing model for Ad Hoc networks. The latter does not require any initialization. Starting from an arbitrary configuration, the network converges to a stable state in a finite time. Our contribution is twofold. We firstly give the formal proof that the stabilization is reached after at most n+2 transitions and requires at most $n{\times}log(2n+{\kappa}+3)$ memory space, where n is the number of network nodes and ${\kappa}$ represents the maximum hops number in the clusters. Furthermore, using the OMNeT++ simulator, we perform an evaluation of our approach. Secondly, we propose an adaptation of our solution in the context of Wireless Sensor Networks (WSNs) with energy constraint. We notably show that our protocol can be easily used for constructing clusters according to multiple criteria in the election of cluster-heads, such as nodes' identity, residual energy or degree. We give a comparison under the different election metrics by evaluating their communication cost and energy consumption. Simulation results show that in terms of number of exchanged messages and energy consumption, it is better to use the Highest-ID metric for electing CHs.

An Analysis on Teaching Quadrilaterals in Elementary School Mathematics Textbooks (초등학교 수학 교과서에 나타난 사각형 지도 방법에 대한 분석)

  • Kim, Hyun-Jeong;Kang, Wan
    • Education of Primary School Mathematics
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    • v.11 no.2
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    • pp.141-159
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    • 2008
  • The purpose of this study id to delve into how elementary mathematics textbook deal with the quadrilaterals from a view of Didactic Transposition Theory. Concerning the instruction period and order, we have concluded the following: First, the instruction period and order of quadrilaterals were systemized when the system of Euclidian geometry was introduced, and have been modified a little bit since then, considering the psychological condition of students. Concerning the definition and presentation methods of quadrangles, we have concluded the following: First, starting from a mere introduction of shape, the definition have gradually formed academic system, as the requirements and systemicity were taken into consideration. Second, when presenting and introducing the definition, quadrilaterals were connected to real life. Concerning the contents and methods of instruction, we have concluded the following: First, the subject of learning has changed from textbook and teachers to students. Second, when presenting and introducing the definition, quadrilaterals were connected to real life. Third, when instructing the characteristics and inclusive relation, students could build up their knowledge by themselves, by questions and concrete operational activities. Fourth, constructions were aimed at understanding of the definition and characteristics of the figures, rather than at itself.

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Clinical Evaluation of Human Papillomavirus DNA Genotyping Assay to Diagnose Women Cervical Cancer

  • Kim, Sung-Hyun;Lee, Dong-Sup;Kim, Yeun;Kim, Gee-Hyuk;Park, Sang-Jung;Choi, Yeon-Im;Kim, Tae-Ue;Park, Kwang-Hwa;Lee, Hye-Young
    • Biomedical Science Letters
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    • v.18 no.2
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    • pp.123-130
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    • 2012
  • In this study, we evaluated the human papillomavirus (HPV) genotyping test called MolecuTech REBA HPV-$ID^{(R)}$ (YD Diagnostics, Seoul, Korea) for 704 women who also had cervical cytological evaluations by Thin Prep. The infection rate of high-risk HPV genotypes was 56.6% in patients with normal cytology, 59.8% in those with benign, low-grade squamous intraepithelial lesions, 51.4% in those with atypical squamous cells of uncertain significance, 92.3% in those with high-grade squamous intraepithelial lesions, and 94.1% in those with squamous cell carcinoma or adenocarcinoma. HPV 16 was the most common genotype detected in any lesion, followed by HPV 53, 58, 33, 52, 45, 31, and 35, in order. The HPV DNA test with PCR-REBA is a very highly sensitive, but less specific, method. The infection rates and HPV genotype distribution of non-Korean people versus people from South Korea showed regional differences.

Short-term Glycemic Control and the Related Factors in Association with Compliance in Diabetic Patients (당뇨병 환자의 치료순응도에 따른 단기간 혈당조절정도와 관련 요인)

  • Kim, Gui-Young;Kim, Bo-Wan;Park, Jae-Yong
    • Journal of Preventive Medicine and Public Health
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    • v.33 no.3
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    • pp.349-363
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    • 2000
  • Objectives : Generally, it seemed that the therapeutic result in diabetic patients was changed by compliance. This study was conducted on the basis of assumption that the therapeutic result id diabetic patients could control according to compliance. This study was conducted to analyze the related factors in association with compliance to drug, diet and exercise therapy. Methods : 224 diabetic patients in Kyungpook National University Hospital were selected through the interviews and HbA1c values from 1 Jan. to 28 Feb.1997. The drug compliance was tested by regularity of drug administration, the diet compliance was tested by restriction of food, exactly allocation, balance of nutrient, measuring food and the exercise compliance was tested by regularity of exercise per day. We assessed compliance by percentage, $x^2-test$ and generalized logit regression model(method:enter). Results : The significant variable was the satisfaction to medical personnels in drug, the knowledge to disease in diet, the participation of the diabetic education in exercise therapy and the satisfaction to medical personnels in HbA1c. Using the generalized logit model(method : enter) in compliance change, the significant variables were the satisfaction to medical personnels and the complication in drug; the significant variables were the age at the first diagnosis, the family history, the concern of health, the knowledge of disease, the self-exertion for therapy and the complication in diet: the only significant variable was the gender in exercise therapy. Conclusions : The degree of glycemic control in diabetic patients was influenced by compliance. In order to improve patient's compliance, we must foster the knowledge on the diseases, lead participation for diabetic education. Because the satisfaction to medical personnels was the important variables, we must build up good relationship between doctors and patients.

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Effect of Low Molecular Weight Silk Fibroin on the Inhibition of Tyrosinase Activity

  • Kang, Gyung Don;Lee, Ki Hoon;Shin, Bong Seob;Nahm, Joong Hee;Park, Young Hwan
    • International Journal of Industrial Entomology and Biomaterials
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    • v.9 no.1
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    • pp.29-33
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    • 2004
  • Low molecular weight silk fibroin (LMSF), which was prepared by hydrolysis of silk fibroin using high-temperature and high-pressure method, was found to inhibit the oxidation of L-3,4,-dihydroxyphenylalanine (L-DOPA) catalyzed by mushroom tyrosinase (EC 1.14.18.1). LMSF contained mostly free amino acids such as L-glycine, L-alanine, and L-serine and oligopeptides, mainly glycine-alanine dimer. As a result of analyzing the inhibition kinetics from Lineweaver-Burk plots, L-glycine and glycine-alanine dimer showed noncompetitive behavior while uncompetitive behavior was observed in L-alanine, and L-serine. When weight percent concentration of ${ID_50}$ was compared, L-glycine was most effective on the inhibition and LMSF was also good enough for the inhibition effect of tyrosinase activity. LMSF showed a mixed-type inhibition and the inhibitory mechanism of LMSF might be caused by free amino acids and oligopeptides. As a result of spectroscopic observation with time, initial rate of increase of DOPAchrome decreased remarkably and the time to reach maximum absorbance increased as an increase of the concentration of L-glycine, meaning that L-glycine made itself mainly responsible for the formation of chelate with ${Cu^2+}$ in tyrosinase. However, in case of L-alanine, L-serine, and especially glycine-alanine dimmer, the production of DOPAchrome after an arrival at maximum absorbance decreased, indicating the production of adducts through the reaction with DOPAquinone.

Dynamic Lung Compliance in Normal Subjects Measured by Pneumatograph (Pneumotachograph 로 측정한 건강인의 동적 폐 Compliance)

  • 이성행
    • Journal of Chest Surgery
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    • v.10 no.2
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    • pp.195-204
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    • 1977
  • Dynamic lung compliance was measured in healthy ten young[mean age, 26 years] male and five young[mean age, 25 years] female. Lung volume was integrated of the rate of flow signal which was obtained by using pneumotachograph and differential pressure transducer[PM 5, Statham]. Intrapleural pressure was measured as that of intraesophagel pressure. Esophageal ballon, 15. 5cm in length, 4ml of luminal capacity and made of thin latex, was connected to the polyethylene tube that had 12-14 side holes and was of 1.5mm of ID. Transpulmonary pressure was traced by means of differential pressure transducer[PM 131, Statham] to which connected the esophageal balloon catheter and connection tube from mouth piece. Lung volume and transpulmonary pressure were photographed by cathode ray oscilloscope camera while the subjects were breathing spontaneously. Dynamic lung compliance loop was displayed on single trace monitor and subtraction was performed for the quasi-static hysteresis. Dynamic lung compliance was measured, 1. by plotting the pressure-volume relationship 2. from the subtracted pressure-volume loop. Results were as follows. 1. Dynamic lung compliances measured by plotting of healthy young male and female were $0.202{\pm}0.06$ and $0.190{\pm}0.023L/cm$ $H_2O$ respectively. 2. When measured from subtraction loop, dynamic lung compliance for male and female were $0.327{\pm}0.107$, and $0.27{\pm}0.06L/cm$ $H_2O$ respectively. 3. Dynamic chest wall and total respiratory system compliance were also measured. 4. Dynamic lung compliance by plotting appeared to be essentially same when compared to that of static compliance reported previously from our laboratory, however, that obtained from subtraction loop revealed higher values than the compliances obtained by plotting and that of static compliance.

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