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Antioxidant and Antimutagenic Activity of Solvent-Fractionated Layers of Colored Rice Bran  

Kang, Mi-Young (Department of Food Science and Nutrition, Kyungpook National University)
Shin, Soo-Young (Department of Food Science and Nutrition, Kyungpook National University)
Nam, Seok-Hyun (Department of Biological Science, Ajou University)
Publication Information
Korean Journal of Food Science and Technology / v.35, no.5, 2003 , pp. 951-958 More about this Journal
Abstract
Twenty-four cultivars of colored rice seeds were collected inside and outside Korea, and the antioxidant and antimutagenic activity was determined for the solvent-fractionated layers of their bran parts lipid soluble fraction, pigment containing fraction, and pigment component per se. As the serial organic solvent extraction proceeded, the overall tendency of antioxidant activities declined with increased chemical homogeneity of each fraction. This markedly showed the low antioxidativities of the pigment components from LK 1-3-6-12-1-1 and Gillimhukmi. Even all the colored rice cultivars, with considerable antimutagenic activity in 70% ethanolic extract, exhibited mutagenicity when measured with its pigment containing fraction (wx 124-163-45-7-1-1-1 and LKlB-2-1-1 being the strongest). The pigment content in each colored rice seeds decreased in the order of IR 17491-5-4-3-3>LK 1-3-6-12-1-1>LK 1D-2-12-1>RGS No.336, Elwee. In addition, a substantial difference in both chemical composition of the constituents and its amount could be found between the colored rice and cooking rice cultivars. This revealed that, compared to cooking rice, major components of organic solvent fractions from colored rice probably have long hydrocarbon chain moieties.
Keywords
colored rice; organic solvent extraction fraction; pigment fraction; antioxidativity; antimutagenicity;
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1 Tsuda, T., Watanabe, M., Ohshima, K., Norinobu, S, Kawakishi, S., Choi, S.W. and Osawa, T. Antioxidative activity of the anthocyanin pigments cyanidin 3-O-$\beta$-D-glucoside and cyamdin. J. Agric. Food Chem. 42: 2407-2411 (1994)   DOI   ScienceOn
2 Kang, M.Y., Choi, Y.H., Nam, S.H. Inhibitory mechanism of colored rice bran extract against mutagenicity induced by chemical mutagen Mitomycin C. Agric. Chem. Biotechnol. 39: 424-429 (1996)
3 Choi, S,W., Nam, S.H. and Choi, H.C. Antioxidative activity of ethanolic extracts of rice brans. Foods Biotechnol. 5: 305-309 (1996)
4 Quilladet, P., Huisaman, O. and Hofnung, M. SOS chromotest, a direct assay of induction of an SOS function in Eschericha coli K-12 to measure genotoxicity. Proc. Natl. Acad. Sci. USA 79: 5971-5980 (1982)   DOI   ScienceOn
5 Adama, R.P. Identification of Essential oil Components by Gas Chromatography/Mass Spectrometry. Allured Publishing Co., IL, USA (1995)
6 Nam, S.H. and Kang, M.Y. Comparison of effect of rice bran extracts of the colored rice cultivars on carcinogenesis. Agric. Chem. Biotechnol. 41: 78-83 (1998)
7 Kang, M.Y., Choi, Y.H. and Nam, S.H. Inhibitory mechanism of colored rice bran extract against mutagenicity induced by chemical mutagen Mitomycin C. Agric. Chem. Biotechnol. 39: 424-429 (1996)
8 Kim, D.W., Eun, J.B. and Rhee, C.O. Cooking condition and textural changes of cooked rice added with black rice. Korean J. Food Sci. Technol. 30: 562-568 (1998)
9 Kovarts, E. Gas chromatographic characterization of organic substance in the retention index system. Adv. chromatogr, 1: 229-247 (1965)
10 Ramarathnam, N., Osawa, T., Namiki, M. and Kawakishi, S. Chemical studies on novel antioxidants I. Isolation, fractionation and partial characterization. J. Agric. Food Chem. 36: 723-727 (1988)
11 Yen, C.G. and Chen, H.Y. Antioxidative activity of various tea extracts in relation to their antimutagenisity. J. Agric. Food Chem. 43: 27-32 (1995)   DOI   ScienceOn
12 Kim, S.S., Kim, S.Y. and Lee, W.J. Chracteristics of germinated colored rice as a potential raw material of Shikhe. Korean J. Food Sci. Technol. 30: 1092-1096 (1998)
13 Choi, S.W., Kang, W.W. and Osawa, T. Isolation and identification of anthocyanin pigments in black rice. Foods Biotechnol. 3: 131-135 (1994)
14 Nam, S.H. and Kang, M.Y. In vitro inhibitory effect of colored rice bran extracts carcinogenecity. Agric. Chem. Biotechnol. 40: 307-312 (1997)
15 Nam, S.H. and Kang, M.Y. In vitro inhibitory effect of colored rice bran extracts carcinogenecity. Agric. Chem. Biotechnol. 40: 307-312 (1997)