• Mycobiology
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• 제51권2호
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• pp.109-113
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• 2023

Auricularia is one of the broadly cultivated edible mushrooms in Korea. Most of the Korean Auricularia strains used for cultivation and breeding are known as A. auricula-judae. Recently, this species has been reported to belong to a species complex. Therefore, this study was carried out to genetically clarify the bred and cultivated Korean A. auricula-judae strains. The internal transcribed spacer (ITS) and IGS1 rDNA region sequences were determined from 10 A. auricula-judae strains by PCR and sequencing. Variation in the nucleotide sequence and sequence length of the two rDNA regions were found among the seven A. auricula-judae strains. A maximum-likelihood (ML) phylogenetic tree based on the ITS sequences clearly placed all the 10 Korean A. auricula-judae strains in the A. heimuer clade of the A. auriculajudae complex. A. heimuer is diverged from A. auricula-judae. An ML phylogenetic tree based on the IGS1 sequences revealed the close relationship between Korean A. heimuer strains to Chinese A. heimuer strains. But each strain could be distinguishable by the IGS1 sequence. Furthermore, progeny strains in the seven Korean strains could be differentiated from their parental strains by the IGS1 sequence based phylogenetic tree. Our results are expected to be used to complement the distinction of domestic Auricularia cultivars.

• Journal of Plant Biotechnology
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• 제39권1호
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• pp.75-80
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• 2012

$Kalopanax$ $pictus$ is a long-lived deciduous perennial tree in the family Araliaceae mainly distributed in the East Asia. In Korea, this species is of ecological and medical importance. Because typical populations of this species are small and distributed in patches, $K.$ $pictus$ has been considered as a narrow habitat species. To understand the genetic diversity and population structure of this species, the sequence variation of the nuclear ribosomal DNA (nrDNA) internal transcribed spacer (ITS) region was analyzed among 18 different $K.$ $pictus$ populations in the present investigation. The nrDNA ITS sequences of Korean populations investigated in this study showed identical of 616 bp in length, and no any nucleotide variation was found in the entire nrDNA ITS region sequence. This result suggested that the $K.$ $pictus$ populations in Korea might belong to the same isolate, and no mutation was found in the nrDNA ITS region. Compared with other known ITS sequence sources from $K.$ $pictus$ populations, only four variable nucleotide sites were found within the entire ITS region. Very narrow genetic diversity appearing in the population level of $K.$ $pictus$ makes us hypothesize that their relatively isolated habitats. The long-lived traits might be one main reason. However, another probability was that the nr-DNA ITS region might be noneffective in classifying populations of $K.$ $pictus$. Thus, to further understand the phylogenetic relationship of $K.$ $pictus$ populations, more samplings should be performed based on more DNA sequences.

• 한국정밀공학회:학술대회논문집
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• 한국정밀공학회 2010년도 춘계학술대회 논문집
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• pp.1329-1330
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• 2010

• 대한전기학회논문지
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• 제40권4호
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• pp.402-406
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• 1991

In this paper, we find a set of conditions on weights under which a recursive weighted median filter preserves monotone or localy monotone sequence and under which any input sequence converges to a locally monotone sequence after a finite number of passes.

• 한국ITS학회 논문지
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• 제7권5호
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• pp.53-63
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• 2008

교본 연구는, 구간속도 검지를 위한 기존의 방법인 프로브차량 방식과 차량 번호판 인식 방식의 문제점을 보완할 수 있는 대안으로써, 도로 구간 시.종점에서의 차량 시퀀스 패턴을 이용하여 구간속도 검지가 가능토록 하는 알고리즘을 개발, 제시하였다. 본 알고리즘은 구간 시.종점에서의 차량들을 '차종 순차(Precedence)패턴을 순서대로 나열한 일정한 길이의 시퀀스 그룹'으로 인식하고, 종점에서의 특정 시퀀스에 대응하는, 시점에서의 시퀀스를 탐색하여 가장 유사도가 높은 시퀀스를 동일 그룹으로 간주하여 해당 구간의 통행 시간을 산출하였다. 유사도 비용의 정의에 따라 세 가지의 모델을 제시하였으며, 차량 유출입에 의한 이상치를 제거하고 가공함으로써 정보제공 주기에 가장 적합한 구간 대표 통행시간을 산출할 수 있도록 하였다. 컴퓨터 모의 실험을 통해 구간길이와 통과차량 수를 증가시키면서 차종별, 시.종점의 시퀀스 길이별로 반복 시뮬레이션 한 결과, 평균 최대 오차율 3.46%로서 현장 적용성에서 뛰어난 가능성을 보였다.

• 한국버섯학회지
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• 제8권1호
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• pp.27-32
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• 2010

본 시험은 국내외에서 수집한 꽃송이버섯균 22균주에 대하여 분자생물학적 유연관계를 분석하고자 하였다. 수집균주의 ribosomal DNA의 ITS 영역에 대한 cleaved amplified polymorphic sequence (CAPS) 분석 결과, KACC50866은 다른 균주들과 20%이하의 유연관계를 나타내었으며 나머지 균주들은 90% 이상의 유연관계를 보이면서 4그룹으로 구분되었다. 따라서 이들의 세분화된 분자생물학적 구분을 위하여 rDNA ITS 영역의 염기서열분석을 하여 구분하여 본 결과 KACC50866 균주는 다른 꽃송이버섯균과 유연관계가 매우 낮은 것으로 나타났다. 그리고 나머지 21개 균주는 같은 그룹으로 구분되어 있어 같은 종으로 생각할 수 있으나, 이들을 좀더 세분하기 위해서는 미토콘드리아의 유전자 서열 분석 등이 병행되어야 할 것으로 판단된다.

• Molecules and Cells
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• 제25권4호
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• pp.510-522
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• 2008

To construct the molecular systematics of the genus Megoura (Hemiptera: Aphididae), DNA based-identification was performed using four mitochondrial and three nuclear DNA regions: partial cytochrome c oxidase I (COI), partial tRNA-leucine + cytochrome c oxidase II (tRNA/COII), cytochrome b (CytB), partial 12S rRNA + tRNA-valine + 16S rRNA (12S/16S), elongation factor-1 alpha ($EF1{\alpha}$), and the internal transcribed spacers 1 and 2 (ITS1, ITS2). Pairwise sequence divergences between taxa were compared, and phylogenetic analyses were performed based on each DNA region separately, and the combined datasets. COI, CytB, $EF1{\alpha}$, ITS1, and ITS2 were relatively effective in determining species and resolving their relationships. By contrast, the sequences of tRNA/COII and 12S/16S were not able to separate the closely related species. CytB and $EF1{\alpha}$ gave better resolution with higher average sequence divergences (4.7% for CytB, 5.2% for $EF1{\alpha}$). The sequence divergence of COI (3.0%) was moderate, and those of the two ITS regions (1.8% for ITS1, 2.0% for ITS2) were very low. Phylogenetic trees were constructed by minimum evolution, maximum parsimony, maximum likelihood, and Bayesian phylogenetic analyses. The results indicated that the phylogenetic relationships between Megoura species were associated with their host preferences. Megoura brevipilosa and M. lespedezae living on Lespedeza were closely related, and M. nigra, monophagous on Vicia venosa, was rather different from M. crassicauda, M. litoralis, and M. viciae, which are oligophagous on Lathyrus and Vicia. The three populations of M. crassicauda formed a clade separated from M. litoralis and M. viciae. Nevertheless M. litoralis and M. viciae, which are morphologically similar, were not separated due to negligible sequence divergence. We discuss the phylogenetic relationships of the Megoura, and the usefulness of the seven DNA regions for determining the species level phylogeny of aphids.

• Genomics & Informatics
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• 제4권4호
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• pp.173-181
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• 2006

Pattern finding is one of the important tasks in a protein or DNA sequence analysis. Alignment is the widely used technique for finding patterns in sequence analysis. BLAST (Basic Local Alignment Search Tool) is one of the most popularly used tools in bio-informatics to explore available DNA or protein sequence databases. BLAST may generate a huge output for a large sequence data that contains various sequence patterns. However, BLAST does not provide a tool to summarize and analyze the patterns or matched alignments in the BLAST output file. BLAST lacks of general and robust parsing tools to extract the essential information out from its output. This paper presents a pattern summary system which is a powerful and comprehensive tool for discovering pattern structures in huge amount of sequence data in the BLAST. The pattern summary system can identify clusters of patterns, extract the cluster pattern sequences from the subject database of BLAST, and display the clusters graphically to show the distribution of clusters in the subject database.

• 한국수학교육학회지시리즈A:수학교육
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• 제14권1호
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• pp.22-26
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• 1975

In this paper, we define a differential module and study its properties. In section 2, as for propositions, Ive research some properties, directsum, isomorphism of factorization, exact sequence of derived modules. And then as for theorem, I try to present the following statement, if the sequence of homomorphisms of differential modules is exact. Then the sequence of homomorphisms of Z(X) is exact, also the sequence of homomorphisms of Z(X) is exact. According to the theorem, as for Lemma, we consider commutative diagram between exact sequence of Z(X) and exact sequence of Z'(X) . As an immediate consequence of this theorem, we obtain the following result. If M is an arbitrary module and the sequence of homomorphisms of the modules Z(X) is exact, then the sequence of their tensor products with the trivial endomorphism is semi-exact.

• 한국CDE학회논문집
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• 제9권2호
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• pp.158-163
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• 2004

Curve tessellation, which generates a sequence of points from a curve, is very important for curve rendering on a computer screen and for NC machining. For the most case the sequence of discrete points is used rather than a continuous curve. This paper deals with a method of tessellation by calculating the maximal deviation of a curve. The maximal deviation condition is introduced to find the point with the maximal deviation. Our approach has two merits. One is that it guarantees satisfaction of a given tolerance, and the other is that it can be applied in not only a polynomial curve but its offset. Especially the point sequence generated from an original curve can cause over-cutting in NC machining. This problem can be solved by using the point sequence generated from the offset curve. The proposed method can be applied for high-accuracy curve tessellation and NC tool-path generation.