Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Genetic diversity of Kalopanax pictus populations in Korea based on the nrDNA ITS sequence

  • Sun, Yan-Lin (School of Life Sciences, Ludong University) ;
  • Lee, Hak-Bong (Department of Forest Resources, Kangwon National University) ;
  • Kim, Nam-Young (Department of Forest Resources, Kangwon National University) ;
  • Park, Wan-Geun (Department of Forest Resources, Kangwon National University) ;
  • Hong, Soon-Kwan (Department of Bio-Health Technology, College of Biomedical Science, Kangwon National University)
  • Received : 2011.12.30
  • Accepted : 2012.01.15
  • Published : 2012.03.31
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Abstract

$Kalopanax$ $pictus$ is a long-lived deciduous perennial tree in the family Araliaceae mainly distributed in the East Asia. In Korea, this species is of ecological and medical importance. Because typical populations of this species are small and distributed in patches, $K.$ $pictus$ has been considered as a narrow habitat species. To understand the genetic diversity and population structure of this species, the sequence variation of the nuclear ribosomal DNA (nrDNA) internal transcribed spacer (ITS) region was analyzed among 18 different $K.$ $pictus$ populations in the present investigation. The nrDNA ITS sequences of Korean populations investigated in this study showed identical of 616 bp in length, and no any nucleotide variation was found in the entire nrDNA ITS region sequence. This result suggested that the $K.$ $pictus$ populations in Korea might belong to the same isolate, and no mutation was found in the nrDNA ITS region. Compared with other known ITS sequence sources from $K.$ $pictus$ populations, only four variable nucleotide sites were found within the entire ITS region. Very narrow genetic diversity appearing in the population level of $K.$ $pictus$ makes us hypothesize that their relatively isolated habitats. The long-lived traits might be one main reason. However, another probability was that the nr-DNA ITS region might be noneffective in classifying populations of $K.$ $pictus$. Thus, to further understand the phylogenetic relationship of $K.$ $pictus$ populations, more samplings should be performed based on more DNA sequences.

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References

  1. Baldwin BG, Sanderson MJ, Porter JM, Wojciechowski MF, Campbell CS, Donoghue MJ (1995) The ITS region of nuclear ribosomal DNA: a valuable source of evidence on angiosperm phylogeny. Ann Missouri Bot Gard 82:247-277 https://doi.org/10.2307/2399880
  2. Bayer RJ (1990) Patterns of clonal diversity in the Antennaria rosea (Asteraceae) polyploidy agamic complex. Am J Bot 77:1313-1319 https://doi.org/10.2307/2444591
  3. Coleman AW (2003) ITS2 is a double-edged tool for eukaryote evolutionary comparisons. Trends Genet 19:370-375 https://doi.org/10.1016/S0168-9525(03)00118-5
  4. Doyle JJ, Doyle JL (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull 19:11-15
  5. Hinrikson HP, Hurst SF, Lott TJ, Warnock DW, Morrison CJ (2005) Assessment of ribosomal large-subunit D1-D2, internal transcribed spacer 1 and internal transcribed spacer 2 regions as targets for molecular identification of medically important Aspergillus species. J Clin Microbiol 43:2092-2103 https://doi.org/10.1128/JCM.43.5.2092-2103.2005
  6. Hu WC, Lee SK, Jung MJ, Heo SI, Hur JH, Wang MH (2010) Induction of cell cycle arrest and apoptosis by the ethyl acetate fraction of Kalopanax pictus leaves in human colon cancer cells. Bioresource Technol 101:9366-9372 https://doi.org/10.1016/j.biortech.2010.06.091
  7. Huh MK (2006) Comparison of genetic diversity and relationships of genus Kalopanax using ISSR markers. J Life Sci 16: 740-745 https://doi.org/10.5352/JLS.2006.16.5.740
  8. Huh MK, Jung SD, Moon HK, Kim SH, Sung JS (2005) Comparison of genetic diversity and population structure of Kalopanax pictus (Araliaceae) and its thornless variant using RAPD. Korean J Med Crop Sci 13:69-74
  9. Jorgenson RA, Cluster PD (1988) Modes and tempos in the evolution of nuclear ribosomal DNA: new characters for evolutionary studies and new markers for genetic and population studies. Ann Missouri Bot Gard 75:1238-1247 https://doi.org/10.2307/2399282
  10. Jung SD, Hong JH, Bang KH, Huh MK (2004) Morphological analysis on the Kalopanax pictus (Araliaceae) of Korean populations. J Life Sci 14:400-405 https://doi.org/10.5352/JLS.2004.14.3.400
  11. Jung SD, Huh HW, Hong JH, Choi JS, Chun HS, Bang KH, Huh MK (2003) Genetic diversity and population structure of Kalopanax pictus (Araliaceae). J Plant Biol 46:255-262 https://doi.org/10.1007/BF03030372
  12. Lee YN (1997) Flora of Korea. Kyo-Hak Publishing Co., Seoul, Korea
  13. Li DW, Lee EB, Kang SS, Hyun JE, Whang WK (2002) Activity-guided isolation of saponins from Kalopanax pictus with anti-inflammatory activity. Chem Pharm Bull 50:900-903 https://doi.org/10.1248/cpb.50.900
  14. Namba T (1994) The Encyclopedia of Wakan-Yaku (Traditional Sino-Japanese Medicine) with Color Pictures, 2nd ed., Hoikusha Co. Ltd., Osaka, Japan.
  15. Park HJ, Kwon SH, Lee JH, Lee KH, Miyamoto KI, Lee KT (2001) Kalopanaxsaponin A is a basic saponin structure for the anti-tumor activity of hederagenin monodesmosides. Planta Med. 67:118-121 https://doi.org/10.1055/s-2001-11516
  16. Park HJ, Nam JH, Jung HJ, Kim WB, Park KK, Chung WY, Choi JW (2005) In vivo antinociceptive antiinflamatory and antioxidative effects of leaf and stem bark of Kalopanax pictus in rats. Kor J Pharmacogn 36:318-323
  17. Sudheer Pamidimarri DVN, Chattopadhyay B, Reddy MP (2009) Genetic divergence and phylogenetic analysis of genus Jatropha based on nuclear ribosomal DNA ITS sequence. Mol Biol Rep 36:1929-1935 https://doi.org/10.1007/s11033-008-9401-6
  18. Sun YL, Hong SK (2011) Genetic diversity and phylogenetic analysis of genus Paeonia based on nuclear ribosomal DNA ITS sequence. J Plant Biotechnol 38:234-240 https://doi.org/10.5010/JPB.2011.38.3.234
  19. Sun YL, Park WG, Kwon OW, Hong SK (2010a) The internal transcribed spacer rDNA specific markers for identification of Zanthoxylum piperitum. Afr J Biotechnol 9:6027-6039
  20. Sun YL, Park WG, Kwon OW, Hong SK (2010b) Ribosomal DNA internal transcribed spacer 1 and internal transcribed spacer 2 regions as targets for molecular identification of medically important Zanthoxylum schinifolium. Afr J Biotechnol 9: 4661-4673
  21. Sun YL, Wang D, Kwon OW, Kim YS, Park WG, Hong SK (2011b) Phylogeny and evolution of Carpinus based on nrDNA ITS region sequences. BMEI 1528-1532
  22. Sun YL, Wang D, Lee HB, Park WG, Kwon OW, Hong SK (2011a) Phylogeny of Korean Hornbeam (Carpinus turczaninovii) based on nuclear ribosomal ITS sequence. Afr J Biotechnol (in press)
  23. Wen J, Lee CH, Lowry PP II, Hiep NT (2003) Inclusion of the Vietnamese endemic genus Grushvitzkya in Brassaiopsis (Araliaceae): evidence from nuclear ribosomal ITS and chloroplast ndhF sequences. Bot J Linn Soc 142 (4), 455-463 https://doi.org/10.1046/j.1095-8339.2003.00188.x
  24. White TJ, Bruns T, Lee S, Taylor J (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ (eds) PCR protocols-a guide to methods and applications. Academic Press, San Diego, Calif, pp 315-322

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