• Title/Summary/Keyword: ITS region

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Blastobotrys illinoisensis, an Unrecorded Anamorphic Yeast Strain Isolated from the Gut of the Earthworm Eisenia fetida

  • Ji Yun Son;Myung Kyum Kim
    • The Korean Journal of Mycology
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    • v.50 no.4
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    • pp.373-381
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    • 2022
  • Strain E4, an unrecorded species of dimorphic fungi, was isolated from the gut of earthworms collected in Gyeonggi Province, South Korea. Nucleotide sequence analysis of the D1/D2 region of the large subunit (LSU) rRNA gene and the internal transcribed spacer (ITS) region revealed that this species is a member of the genus Blastobotrys, Blastobotrys illinoisensis. Strain E4 differed from its closest known species, B. mokoenaii and B. malaysiensis, by harboring 3-5 and 12-14 nucleotide substitutions in the D1/D2 and ITS regions, respectively. Phylogenetic analysis based on concatenated sequences of the D1/D2 region of the LSU rRNA gene and the ITS region also indicated that strain E4 belongs to the Blastobotrys clade and is distinct from other related species in the clade. The previously unreported isolate could be distinguished from closely related species by its inability to ferment carbon sources. To our knowledge, this is the first report on the isolation of Blastobotrys species from the gut of earthworms in Korea. The strain used was E4 (=KCTC 27831=JCM 33428).

A Basic Study on the Fire Flame Extraction of Non-Residential Facilities Based on Core Object Extraction (핵심 객체 추출에 기반한 비주거 시설의 화재불꽃 추출에 관한 기초 연구)

  • Park, Changmin
    • Journal of Korea Society of Digital Industry and Information Management
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    • v.13 no.4
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    • pp.71-79
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    • 2017
  • Recently, Fire watching and dangerous substances monitoring system has been being developed to enhance various fire related security. It is generally assumed that fire flame extraction plays a very important role on this monitoring system. In this study, we propose the fire flame extraction method of Non-Residential Facilities based on core object extraction in image. A core object is defined as a comparatively large object at center of the image. First of all, an input image and its decreased resolution image are segmented. Segmented regions are classified as the outer or the inner region. The outer region is adjacent to boundaries of the image and the rest is not. Then core object regions and core background regions are selected from the inner region and the outer region, respectively. Core object regions are the representative regions for the object and are selected by using the information about the region size and location. Each inner region is classified into foreground or background region by comparing its values of a color histogram intersection of the inner region against the core object region and the core background region. Finally, the extracted core object region is determined as fire flame object in the image. Through experiments, we find that to provide a basic measures can respond effectively and quickly to fire in non-residential facilities.

Secondary Structure of the Ribosomal Internal Transcribed Spacer (ITS) Region of Hypsizygus marmoreus (느티만가닥버섯의 ITS (internal transcribed spacer) 영역의 2차구조 분석)

  • Woo, Ju-Ri;Yoon, Hyeokjun;You, Young-Hyun;Lee, Chang-Yun;Kong, Won-Sik;Kim, Jong-Guk
    • Journal of Life Science
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    • v.23 no.10
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    • pp.1260-1266
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    • 2013
  • The ribosomal DNA (rDNA) clusters of Hypsizygus marmoreus 3-10 and H. marmoreus 1-1 were analyzed in this study. The small subunit (SSU) and intergenic spacer 2 (IGS 2) was partially sequenced. The internal transcribed spacer 1 (ITS 1), 5.8S, internal transcribed spacer 2 (ITS 2), large subunit (LSU), intergenic spacer 1 (IGS 1), and 5S were completely sequenced. The rDNA clusters of H. marmoreus 3-10 and H. marmoreus 1-1 were 7,049 bp in length. The sequence of SSU rDNA, which corresponded to 18S rDNA, was 1,796 bp in length, and the sequence of LSU rDNA, which corresponded to 28S rDNA, was 3,348 bp in length. The ITS region that variable region and IGS region that non-transcribed spacer was 462 bp and 1,290 bp in length. The sequence of 5.8S rDNA and 5S rDNA was 153 bp and 43 bp in length, respectively. The 17 bp of the rDNA cluster in the H. marmoreus 3-10 strain was different to that in the H. marmoreus 1-1 strain, with 2 bp in the SSU, 3 bp in the ITS, 9 bp in the LSU, and 3 bp in the IGS. The analysis of the secondary structure revealed that the ITS regions of H. marmoreus 3-10 and H. marmoreus 1-1 have five stem-loop structures. Interestingly, among these structures, one different nucleotide sequence resulted in a different secondary structure in stem-loop V.

Molecular Identification of Two Strains of Phellinus sp. by Internal Transcribed Spacer Sequence Analysis

  • Shin, Kwang-Soo
    • Mycobiology
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    • v.39 no.4
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    • pp.299-300
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    • 2011
  • Two species of cultivated Phellinus sp. were identified as P. baumii by internal transcribed spacer (ITS) sequence analysis. The fruit bodies of the examined strains were similar to those of naturally occurring strains, having a bracket-like form, yellow-to-orange color, and poroid hymenial surfaces. The DNA sequences of ITS region of both strains showed a homology of 99% with ITS1 to ITS2 sequences of P. (Inonotus) baumii strain PB0806.

A study on the characteristics of end region flow field for partially cavitating hydrofoil (익형에 발생하는 부분 캐비테이션 후연영역 유동장 특성 연구)

  • 문철진;김시영
    • Journal of Advanced Marine Engineering and Technology
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    • v.12 no.1
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    • pp.53-61
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    • 1988
  • Most of the recent for the flow field hydrofoil in partially cavitating condition are the ones which are linearized, and the problem of cavity end region for hydrofoil is not verified. This paper deals with a study on characteristics of end region flow field for partially cavitating hydrofoil by using a characteristics of shear turbulence flow and nonlinear cavity flow theory. The results obtained as follows : 1) Shear layer thickness is decreased gradually going to the end section of hydrofoil. When attack angle is large, it is appeared largely at the region of partial cavitation after its collapsing. 2) The fluctuation velocity of a second-degree relative direction have minimum value at the front of hydrofoil or at the end of hydrofoil. The difference for the validity of attack angle is appeared largely at the surrounding of .chi.$_{e}$ point. 3) The fluctuation velocity of transverse direction decrease from the maximum thickness of cavitation to the end of hydrofoil, but it undergoes largely the effect of pressure recovery. The difference is larger at the region of partial cavitation after its collapsing than at the of hydrofoil. 4) The distribution of Reynolds stress have maximum values at the region of partial cavitation after its collapsing and the end of foil, and the larger attack angle, the larger the distribution of value.e.

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Characteristics of the Resistance Phenotypes by Inducible Resistance Gene ermK and Its Terminator Region Mutants (MLS계 항생제에 대한 유도내성 유전자 ermK 및 그 돌연변이체의 유도내성 표현형)

  • Choi, Sung-Sook;Choi, Eung-Chil
    • YAKHAK HOEJI
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    • v.41 no.4
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    • pp.533-537
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    • 1997
  • The characteristics of the resistance phenotypes of Bacillus subtilis having ermk and its terminator region mutants were determined. Wild type ermK(pEC101) and pECMT109(methylase SD-region mutant) showed typical inducible resistance phenotype. pECMF1(terminator1 region mutant) and pECMT2(terminator2 region mutant) showed constitutive resistance to Kitasamycin but inducible resistance to tylosin. In contrast, pECMT3(terminator1 and terminator2 double mutant) and pECMT309(terminator1, terminator2 and methylase SD region triple mutant) showed constitutive resistance both to kitasamycin and tylosin.

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Genetic Variation of Rice Populations Estimated Using nrDNA ITS Region Sequence

  • Wang, Dong;Hong, Soon-Kwan
    • Korean Journal of Plant Resources
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    • v.27 no.3
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    • pp.249-255
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    • 2014
  • The rice belonging to Oryza sativa is not only has significant economic importance, for it is the major source of nutrition for about 3 billion all around the world. But also plays a vital role as a model organism, because it has a number of advantages to be a model plant, such as efficient transformation system and small genome size. Many methods and techniques have been conducted to attempt to distinguish different Oryza sativa species, such as amplified fragment length polymorphism (AFLP), random amplified polymorphic DNA (RAPD), simple sequence repeat (SSR) and so on. However, studies using sequence analysis of internal transcribed spacer (ITS), a region of ribosomal RNA has not been reported until now. This study was undertaken with an aim to understand the phylogenetic relationships among sixteen isolates of Oryza sativa collected from abroad and fifteen isolates collected from Korea, using ribosomal RNA (rRNA) internal transcribed spacer (ITS) sequences to compare the phylogeny relationships among different Oryza sativa species. The size variation obtained among sequenced nuclear ribosomal DNA (nrDNA) ITS region ranged from 515bp to 1000bp. The highest interspecific genetic distance (GD) was found between Sfejare 45 (FR12) and Anapuruna (FR15). Taebong isolate showed the least dissimilarity of the ITS region sequence with other thirty isolates. This consequence will help us further understanding molecular diversification in intra-species population and their phylogenetic analysis.

ROK Navy's Role for a Confidence Building and Mutual Cooperation on the East Asian Sea (역내 해양협력 및 신뢰증진을 위한 한국 해군의 기여방안)

  • Park, Young-June
    • Strategy21
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    • s.30
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    • pp.143-176
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    • 2012
  • We are witnessing the growing maritime tension on the East Asian sea these days. Each naval powers in the region are competing each other to acquire more advanced naval capabilities. Based upon the rapid economic development, China is actively beefing up its naval capabilities and expand its boundary of naval activities all over the East Asian region. Chinese Navy already unveiled its expansive naval strategy replacing the traditional concept of 'Near-Sea Defense' with the new concept of 'Far-Sea Defense' strategy. In response to potential rival's naval build up, the U.S. is redeploying its naval forces focusing on the Asia-Pacific region. The U.S. enhances its joint naval exercises with the countries in the region, such as Japan, India, Australia and so on. In addition, Washington is devising new naval strategy under the concept of 'Air-Sea Battle' to deter Peking's so-called 'Anti-Access/ Area Denial(A2AD)' strategy. As a close ally of the U.S., Japan also disclosed its clear intention to strengthen the Maritime Self Defense Force(MSDF)'s capabilities by introducing the new concept of 'Dynamic Defense Force' in 2011. Under the new concept, JMSDF is pursuing the additional acquisition of submarines, quasi-aircraft carriers, Aegis-equipped destroyers, etc. Under the new president's strong leadership, Russia is also invigorating the naval build-up. Especially, Russia is fortifying the Pacific Fleet's naval assets by deploying new-type of naval ships such as the Mistral which was imported from France. In the midst of competitive naval build-up among the major naval powers in the region, we are observing the growing maritime conflicts on the East China Sea as well as South China Sea. Those naval conflicts can pose severe threats to our national interests. Maritime conflicts on the East or South China Sea can imperil our sea lanes which will be indispensible for national economic development. Neighboring countries' maritime conflicts also will cast an uncertainty on the path to mobilize international cooperation to resolve the North Korean issues. We should contribute to ease the maritime tension in the region by various ways. First, we should actively galvanize the bilateral maritime dialogue among the major naval powers in the region. Second, we also should take the lead to form a multilateral maritime cooperation mechanism in the region. Above all, we should set the aim to be a peaceful maritime power who can contribute to a building of stable maritime order in the region with a considerable naval power.

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Improved Momentum Exchange Theory for Incompressible Regenerative Turbomachines (I) - Hydraulic Model - (비압축성 재생형 기계에 대한 개선된 운동량 교환 이론 (I) - 수력학적 모델 -)

  • Park Mu Ryong;Chung Myung Kyoon;Yoo Il Su
    • Transactions of the Korean Society of Mechanical Engineers B
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    • v.28 no.10
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    • pp.1238-1246
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    • 2004
  • Momentum exchange theory has been generally used for an analysis of the regenerative turbomachines due to its direct description of the complicate circulatory flow. However, because its application is limited only to linear region and its model equations are incomplete on three variables, it needs further refinements. In the present study it is improved by introducing a central pivot of circulatory flow. Also, by assuming linear circulatory velocity distribution, mean radii of inlet and outlet flows through the impeller are newly suggested. By applying control volume analysis to both linear region and the acceleration region, the governing equation on the circulatory velocity is derived. As a result, systematic performance analysis on the entire region of the incompressible regenerative turbomachines can be carried out based on the proposed model equations.

MERGING AND FRAGMENTATION IN THE SOLAR ACTIVE REGION 10930 CAUSED BY AN EMERGING MAGNETIC FLUX TUBE WITH ASYMMETRIC FIELD-LINE TWIST DISTRIBUTION ALONG ITS AXIS

  • Magara, Tetsuya
    • Journal of The Korean Astronomical Society
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    • v.52 no.4
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    • pp.89-97
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    • 2019
  • We demonstrate the subsurface origin of the observed evolution of the solar active region 10930 (AR10930) associated with merging and breakup of magnetic polarity regions at the solar surface. We performed a magnetohydrodynamic simulation of an emerging magnetic flux tube whose field-line twist is asymmetrically distributed along its axis, which is a key to merging and fragmentation in this active region. While emerging into the surface, the flux tube is subjected to partial splitting of its weakly twisted portion, forming separate polarity regions at the solar surface. As emergence proceeds, these separate polarity regions start to merge and then break up, while in the corona sigmoidal structures form and a solar eruption occurs. We discuss what physical processes could be involved in the characteristic evolution of an active region magnetic field that leads to the formation of a sunspot surrounded by satellite polarity regions.