[KSCI] Korea Science Citation Index Service

http://dx.doi.org/10.4489/KJM.20220041

Blastobotrys illinoisensis, an Unrecorded Anamorphic Yeast Strain Isolated from the Gut of the Earthworm Eisenia fetida

Ji Yun Son (Department of Bio & Environmental Technology, College of Natural Science, Seoul Women's University)
Myung Kyum Kim (Department of Bio & Environmental Technology, College of Natural Science, Seoul Women's University)

Publication Information

The Korean Journal of Mycology / v.50, no.4, 2022 , pp. 373-381 More about this Journal

Abstract

Strain E4, an unrecorded species of dimorphic fungi, was isolated from the gut of earthworms collected in Gyeonggi Province, South Korea. Nucleotide sequence analysis of the D1/D2 region of the large subunit (LSU) rRNA gene and the internal transcribed spacer (ITS) region revealed that this species is a member of the genus Blastobotrys, Blastobotrys illinoisensis. Strain E4 differed from its closest known species, B. mokoenaii and B. malaysiensis, by harboring 3-5 and 12-14 nucleotide substitutions in the D1/D2 and ITS regions, respectively. Phylogenetic analysis based on concatenated sequences of the D1/D2 region of the LSU rRNA gene and the ITS region also indicated that strain E4 belongs to the Blastobotrys clade and is distinct from other related species in the clade. The previously unreported isolate could be distinguished from closely related species by its inability to ferment carbon sources. To our knowledge, this is the first report on the isolation of Blastobotrys species from the gut of earthworms in Korea. The strain used was E4 (=KCTC 27831=JCM 33428).

Keywords

Blastobotrys sp.; D1/D2; Earthworm; ITS (internal transcribed spacer); Yeast;

Citations & Related Records

Reference

1	Dash MC, Senapati BK, Mishra CC. Nematode feeding by tropical earthworms. Oikos 1980;34:322-5. DOI
2	Kale RD, Mallesh BC, Kubra B, Bhagyaraj DJ. Influence of vermicompost application on available micronutrients and selected microbial populations in paddy field. Soil Biol Biochem 1992;24:1317-20. DOI
3	von Klopotek A. Blastobotrys nivea gen. nov., sp. nov. Arch Mikrobiol 1967;58:92-6. DOI
4	Kurtzman CP, Robnett CJ. Molecular relationships among hyphal ascomycetous yeasts and yeast like taxa. Can J Bot 1995;73:S824-30.
5	Kurtzman CP, Robnett CJ. Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences. Anton van Leeuwenhoek 1998;73:331-71. DOI
6	Kurtzman CP. Blastobotrys americana sp. nov., Blastobotrys illinoisensis sp. nov., Blastobotrys malaysiensis sp. nov., Blastobotrys muscicola sp. nov., Blastobotrys peoriensis sp. nov. and Blastobotrys raffinosifermentans sp. nov., unreported anamorphic yeast species. Int J Syst Evol Microbiol 2007;57:1154-62. DOI
7	Kim JY, Jang JH, Park JH, Jung HY, Park JS, Cho SJ, Lee HB, Limtong S, Subramani G, Sung GH, et al. Cellulose degrading basidiomycetes yeast isolated from the gut of grasshopper in Korea. Korean J Microbiol 2018;54:362-8.
8	Kurtzman CP, Fell JW, Boekhout T, Robert V. Methods for isolation, phenotypic characterization and maintenance of yeasts. In: Kurtzman CP, Fell JW, Boekhout T, editors. The Yeasts, A Taxonomic Study, 5th ed, vol. 1. Amsterdam: Elsevier; 2011. p. 87-110.
9	Cubero OF, Crespo A, Fatehi J, Bridge PD. DNA extraction and PCR amplification method suitable for fresh, herbarium-stored, lichenized, and other fungi. Plant Syst Evol 1999;216:243-9. DOI
10	White TJ, Bruns T, Lee S, Taylor JW. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ, editors. PCR Protocols: A Guide to Methods and Applications. New York: Academic Press; 1990. p. 315-22.
11	Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 1997;25:4876-82. DOI
12	Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987;4:406-25.
13	Kumar S, Stecher G, Tamura K. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Bio Evol 2016;33:1870-4. DOI
14	Nei M, Kumar S. Molecular evolution and phylogenetics. Oxford: Oxford University Press; 2000.
15	Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evol 1985;39:783-91. DOI
16	Bahrndorff S, Alemu T, Alemneh T, Lund Nielsen J. The microbiome of animals: implications for conservation biology. Int J Gen 2016;2016:e5304028.
17	Vu D, Groenewald M, Szoke S, Cardinali G, Eberhardt U, Stielow B, De Vries M, Verkleij GJM, Crous PW, Boekhout TJSIM, et al. DNA barcoding analysis of more than 9000 yeast isolates contributes to quantitative thresholds for yeast species and genera delimitation. Stud Mycol 2016;85:91-105. DOI
18	Nouri H, Moghimi H, Vaghei MG, Nasr S. Blastobotrys persicus sp. nov., an ascomycetous yeast species isolated from cave soil. Antonie van Leeuwenhoek 2018;111:517-24. DOI
19	May RM. How many species are there on earth? Sci 1988;241:1441-9. DOI
20	Hosokawa T, Kikuchi Y, Nikoh N, Shimada M, Fukatsu T. Strict host-symbiont co-speciation and reductive genome evolution in insect gut bacteria. PLoS Bio 2006;4:e337.
21	Vandecasteele B, Samyn J, Quataert P, Muys B, Tack FMG. Earthworm biomass as additional information for risk assessment of heavy metal biomagni-fication, a case study for dredged sediment-derived soils and polluted floodplain. Soils Environ Pollut 2004;129:363-75. DOI
22	Martin A, Cortez J, Barosis I, Lavelle P. Les mucus intestinaux de Var de Terre, moteur de leurs interactions avec la microflore. Rev Ecol Biol Sol 1987;24:549-58.
23	Barois I, Lavelle P. Changes in respiration rate and some physico-chemical properties of a tropical soil during transit through Pontoscolex corethrurus (Glossoscolecidae, Oligochaeta). Soil Biol Biochem 1986;18:539-41. DOI
24	Govindarajan B, Prabaharan V. Gut micro-flora of earthworms: a review. Am J of Biol Pharma Res 2014;1:125-30.