• Title/Summary/Keyword: ITS & cpDNA 지역

Search Result 5, Processing Time 0.019 seconds

Genetic relationship of Aloe vera 'Saengjang', a new forma, based on cpDNA and ITS sequence variation (cpDNA와 ITS 염기변이에 근거한 신품종 생장알로에 유전적 상관관계)

  • Srikanth, Krishnamoorthy;Jang, Seon Il;Whang, Sung Soo
    • Korean Journal of Plant Taxonomy
    • /
    • v.44 no.4
    • /
    • pp.250-256
    • /
    • 2014
  • This study was carried out to understand the genetic relationship of three Aloe spp. cultivated in Korea, A. saponaria, A. vera and A. arborescens and a new variant in Korea based on three plastid (matK, trnL-F, rbcL) and one nuclear (ITS regions) DNA barcode markers. A total of 2,420 bp sequence was amplified. Two indels were detected in the trnL region, and also several species specific nucleotide loci were detected in all 29 parsimonious informative sites, and 148 variable sites were detected among four taxa studied while 170 variable and 75 parsimonious sites were detected when other Aloe spp. in worldwide were used. An UPGMA phenogram with 10,000 bootstrap replication showed that the new variant was closest to A. vera. The variant was not morphologically and genetically concurrent with any reported species so far. The clustering of Aloe species were broadly in agreement with previously reported results.

Molecular evolution of cpDNA trnL-F region in Korean Thalictrum L. (Ranunculaceae) and its phylogenetic relationships: Impacts of indel events (한국산 꿩의다리속(미나리아재비과)의 cpDNA trnL-F 지역의 분자진화와 유연관계: Indel events의 영향)

  • Park, Seongjun;Kim, Hyuk-Jin;Park, SeonJoo
    • Korean Journal of Plant Taxonomy
    • /
    • v.42 no.1
    • /
    • pp.13-23
    • /
    • 2012
  • The trnL-F region islocated in the large single-copy region of the chloroplast genome. It consists of the trnL gene, the trnL intron, and the trnL-F IGS. Molecular evolution and phylogenetic relationships in Korean Thalictrum L. were investigated using data from the cpDNA trnL-F region. Bayesian and parsimony analyses of the data set with the gap characteristics recovered well-resolved trees that are topologically similar, with clades supported by some indels evolution. Indel events of cpDNA trnL-F in Korean Thalictrum were interpreted as phylogenetically informative characteristics. Sect. Physocarpum (excluding T. osmorhizoides) was an early-diverging group with in the genus and the remaining section formed strongly supported clades. Korean Thalictrum has various evolutionary patterns, such as the spatial distribution of the nucleotide diversity and transversion-type base substitutions in the trnL-F region.

A phylogenetic analysis of the genus Pilea (Urticaceae) using nrDNA and cpDNA sequences (한국산 물통이속(Pilea) 식물의 nrDNA, cpDNA를 통한 계통분석)

  • Moon, Ae-Ra;Park, Jeong-Mi;Jang, Chang-Gee
    • Korean Journal of Plant Taxonomy
    • /
    • v.45 no.2
    • /
    • pp.158-168
    • /
    • 2015
  • A study of the genus Pilea in Korea including five taxa was carried out using molecular phylogenetic methods. The majority of members of the genus Pilea in Korea are annual herbs, and they live in moist habitats, flowering in summer and fruiting in autumn. The results of a phylogenetic analysis using nrDNA and cpDNA supported the recognition of P. japonica, P. peploides, and P. taquetii. Pilea taquetii from Mt. Sanbangsan in Jeju was nested within P. hamaoi and P. mongolica clade instead of the P. taquetii clade, with P. taquetii from Mt. Jirisan also separated from the P. taquetii clade. This indicates that the separation is not geographical isolation, but is instead related to taxonomic problems. Therefore, further study of the P. taquetii group is necessary.

Molecular Phylogenetic Studies of Korean Hydrocotyle L. (한국산 피막이속(Hydrocotyle L.) 식물의 분자계통학적 연구)

  • Choi, Kyoung-Su;Park, Seon-Joo
    • Korean Journal of Plant Resources
    • /
    • v.25 no.4
    • /
    • pp.490-497
    • /
    • 2012
  • Phylogenetic analyses were conducted to evaluate relationships of 5 taxa of Korean Hydrocotyle, H. spp. found in the Ulleung island including one outgroup (Centella asiatica). The molecular phylogenetic methods based on nuclear ribosomal DNA ITS region and cpDNA trnH-psbA region. Centella asiatica was used outgroup for analysis. As the result, genus Korean Hydrocotyle were grouped by 94% bootstrap value. Korean Hydrocotyle was grouped by four clades; Clade I-H. maritima, H. sibthorpides and H. yabei clade Clade II-H. nepalensis clade clade III-H. ramiflora clade clade IV-H. spp. clade. H. maritima, H. sibthorpides and H. yabei was not distinguished, seperately. H. spp. was distinctly distinguished other Korean Hydrocotyle.

The Identity of the Variation Population of Polygonatum cryptanthum H. Lév. & Vaniot (목포용둥굴레 변이 집단의 실체)

  • Se Ryeong Lee;Chang Gee Jang
    • Proceedings of the Plant Resources Society of Korea Conference
    • /
    • 2022.09a
    • /
    • pp.50-50
    • /
    • 2022
  • 비짜루과 둥굴레속(Asparagaceae: Polygonatum)은 전 세계적으로 약 90여 종이 알려져 있으며, 유럽, 북아메리카, 아시아 등 북반구 온대 지역에 집중적으로 분포한다. 국내 둥굴레속 분류군은 총 16분류군이며, 이중 잎이 호생하고, 난형에서 타원형 모양의 엽질성 포를 가지며, 화피통 내부에 털이 없고, 수술대 표면에 돌기가 나있는 분류군들은 용둥굴레열(series. Bracteata)에 속한다. 그러나 이들은 종간 교잡 또는 주요 기관의 형질 변이가 다양하여 중간형질을 보이는 개체군들에 대한 종 식별에 많은 어려움이 있었다. 경남 창원시에서 채집된 목포용둥굴레(P. cryptanthum) 변이 개체집단는 기존의 목포용둥굴레와 달리 식물체 높이와 화경·소화경이 길며, 포 부착위치의 변이 폭이 넓으며, 포가 타원형이고 밖으로 말리는 습성으로 형태적 차이가 나타났다. 따라서 본 연구에서는 명확한 분류학적 실체를 구명하고자 분자생물학적 계통분석(nrDNA ITS + cpDNA matK, trnK-rps16, rps16, rbcL) 연구를 진행중에 있다.

  • PDF