• 한국지진공학회논문집
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• 제26권2호
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• pp.95-103
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• 2022

In order to evaluate the earthquake safety of equipment in structures, it is essential to analyze the In-Structure Response Spectrum (ISRS). The ISRS has a peak value at the frequency corresponding to the structural vibration mode, but the frequency and amplitude at the peak can vary because of many uncertain parameters. There are several seismic design criteria for ISRS peak-broadening for fixed base structures. However, there are no suggested criteria for constructing the design ISRS of seismically isolated structures. The ISRS of isolated structures may change due to the major uncertainty parameter of the isolator, which is the shear stiffness of the isolator and the several uncertainty parameters caused by the nonlinear behavior of isolators. This study evaluated the effects on the ISRS due to the initial stiffness of the bi-linear curve of isolators and the variation of effective stiffness by the input ground motion intensity and intense motion duration. Analyzing a simplified structural model for isolated base structure confirmed that the ISRS at the frequency of structural mode was amplified and shifted. It was found that the uncertainty of the initial stiffness of isolators significantly affects the shape of ISRS. The variation caused by the intensity and duration of input ground motions was also evaluated. These results suggested several considerations for generating ISRS for seismically isolated structures.

• 한국전산구조공학회논문집
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• 제37권2호
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• pp.111-118
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• 2024

원자력발전소(원전) 시스템 내진성능 평가를 위하여 구조물내응답스펙트럼(ISRS)은 필수적으로 요구된다. 특히, 원전 부지 고유 스펙트럼 변경 시 새로운 ISRS 도출이 요구될 경우 지진 재해석 등의 상당한 비용을 필요로 하게 된다. 따라서 이 연구는 지진 재해석이 필요 없는 ISRS 스케일링 근사 방법에 대한 여러 가지 접근법을 제공한다. 이러한 접근법으로 도출한 ISRS는 정확한 ISRS와 비교한다. 근사 방법의 ISRS 가 원전 주요 시스템 지진응답 및 내진성능에 미치는 영향을 분석한다. 결과적으로 본 연구에서 제시한 ISRS 스케일링 근사 방법은 저주파에서 비교적 유사하게 ISRS를 도출하지만, 고주파에서는 그 정확도가 감소하였다. ISRS 스케일링 근사방법이 시스템 지진응답/내진성능 산출 정확도에 미치는 영향은 방법의 시스템 주요 모드 응답 유사도 산출 정도에 따라 결정된 것을 확인할 수 있었다.

• The Plant Pathology Journal
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• 제16권2호
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• pp.98-104
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• 2000

For the taxonomic evaluaition, 15 strains of the genus Pectobacterium and Erwinia were analyzed for 16S-23S rDNA intergenic spacer regions (ISRs). These species contained two types of ISRs, large and small ISRs. Large ISRs were on the range of 474-569 bp size, and coding transfer $\textrm{RNA}^{11e}$($\textrm{tRNA}^{11e}$) and $\textrm{tRNA}^{Ala}$. Small ISRs were 354-459 bp in length and coding $\textrm{tRNA}^{Glu}$. The sequence variations of two ISRs among species and strains were very high as compared with 16S rRNA gene sequences. By phylogenetic trees on the basis of two ISRs, Pectobacterium ere differentiated into P. carotovorum-P. cactiaidum group and P. chrysanthemi group. However, the taxonomic position of E. cypripedii and E. rhapontici, which were not clear on taxonomic delineation between Pectobacterium and Erwinia, were not clearly resolved on the basis of ISRs.

• The Plant Pathology Journal
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• 제16권5호
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• pp.252-256
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• 2000

The 16S-23S rRNA intergenic spacer regions (ISRs) were sequenced and analyzed to design specific primer for identification of Pectobacterium chrysanthemi. Two types ISRs, large and small ISRs, were identified from three strains (ATCC 11663, KACC 10163 and KACC 10165) of P. chrysanthemi and Pectobacterium carotovorum subsp. carotovorum ATCC 15713.Large ISRs contained transfer RNA-Ile(tRNA$^{Ile}$)and tRNA$^{Ala}$, and small ISRs contained tRNA$^{Glu}$. Size of the small ISRs of P. chrysanthemi ranged on 354-356 bp, while it was 451 bp in small ISR of P. carotovorum subsp. carotovorum ATCC 15713. From hypervariable region of small ISRs, species-specific primer for P. chrysanthemi with 20 bp length (CHPG) was designed from hypervariable region of small ISRs, which was used as forward promer to detect P. chrysanthemi strains with R23-1R produced PCR product of about 260bp size (CHSF) only from P. chrysanthemi strains, not from other Pectobacterium spp. and Erwinia spp. Direct PCR from bacterial cell without extracting DNA successfully amplified a specific fragment, CHSF, from P. chrysanthemi ATCC 11663. The limit of PCR detection was 1${\pm}10^2$ cfu/ml.

• 한국지진공학회논문집
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• 제28권1호
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• pp.1-10
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• 2024

For important structures such as nuclear power plants, In-Structure Response Spectrum (ISRS) analysis is essential because it evaluates the safety of equipment and components installed in the structure. Because most structures are asymmetric, the response can be affected by eccentricity. In the case of seismically isolated structures, this effect can be greater due to the difference between the center of mass of the structure and the center of rigidity of the isolator layer. Therefore, eccentricity effects must be considered when designing or evaluating the ISRS of seismically isolated structures. This study investigated the change of the ISRS of an isolated structure by assuming accidental eccentricity. The variables that affect the ISRS of the isolated structure were analyzed to see what additional impact they had due to eccentricity. The ISRS of the seismically isolated structure with eccentricity was amplified more than when there was non-eccentricity, and it was boosted more significantly in specific period ranges depending on the isolator's initial stiffness and seismic intensity. Finally, whether the displacement requirement of isolators can be applied to the variation of the ISRS due to eccentricity in the design code was also examined.

• Biomolecules & Therapeutics
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• 제18권3호
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• pp.316-320
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• 2010

Pruritus is one of major symptoms in atopic dermatis. The pathophysiological mechanism of pruritus is unclear. The search for pruritogen is important in elucidating the pathophysiological mechanism of pruritus in atopic dermatitis. Glucosylsphingosine (Gsp) is upregulated in the strateum corneum of atopic dermatitis patients. We investigated to determine whether Gsp induces itch-scratch responses (ISRs) in mice. Intradermal administration of Gsp induces ISRs. Gsp dose-dependently induced scratching response at 50-500 nmol/site range. Pretreatment with naltrexone, an opioid $\mu$ receptor antagonist, and capsaicin, a TrpV1 receptor agonist, inhibited Gsp-induced ISRs. Additionally, Gsp-induced ISRs were also suppressed by cyproheptadine, an antagonist of serotonin receptor. These findings suggest that Gsp-induced scratching might be at least partly mediated by capsaicin-sensitive primary afferents, and the opioids receptor systems might be involved in transmission of itch signaling in the central nervous system. Furthermore, our findings suggest that Gsp-induced ISRs may be attributable to the serotonin-mediated pathways and Gsp is not any more one of byproducts of abnormal skin barrier but can lead to induce pruritus, one of typical symptoms of atopic dermatitis.

• 한국수산과학회지
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• 제36권3호
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• pp.239-246
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• 2003

We have examined the 16S-23S rRNA intergenic spacer region (ISR) of Vibrio vulnificus KCTC 2959. ISRs were amplified by primers complementary to conserved regions of 16S and 23S rRNA genes. ISR amplicons were cloned and sequenced. Analysis of the ISR sequences showed that V. vulnificus KCTC 2959 contains five types of polymorphic ISRs. Size of ISRs ranged from 424 to 741 bp in length and the number of tRNA genes ranged from one to four. The ISRs were designated as ISR-E $(tRNA^{Glu}),\;ISR-IA\;(tRNA^{Ile}-tRNA^{Ala})$, ISR-EKV $(tRNA^{Glu}-tRNA^{Lys}-tRNA^{Val})$, ISR-IAV $(tRNA^{Ile}-tRNA^{Ala}-tRNA^{val})$ and ISR-EKAV $(tRNA^{Glu}-tRNA^{Lys}-tRNA^{Ala}-tRNA^{Val})$ based on their tRNA genes. Multiple alignment of representative sequences from different Vibrio species revealed several domains of high sequence variability. We used the sequences of variable domains to design species-specific primer for detection PCR. Specificity of the primers was examined using genomic DNA prepared from 18 different Vibrio species. The results showed that the PCR using primers designed in this study can be used to detect V. vulnificus from other Vibrio species.

• 한국가스학회지
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• 제3권3호
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• pp.17-22
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• 1999

본 연구에서는 PSM, SMS, BS 8800, ISRS 체계 분석을 통하여 시스템 모두를 만족하는 안전$\cdot$보건 경영체제(SHMS)와 환경 경영 체제(ISO 14000)를 통합한 경영체제를 구축하였다. 또한, 통합된 안전$\cdot$보건 경영체제에서 표준 문서 형식을 만들 수 있는 가이드라인 모델을 제시하였다. 더욱이, 본 연구에서 개발한 통합 경영 체제는 화학공장에서의 물적, 인적 재원의 중복성과 비능률성을 감소시킬 수 있다.

• 한국화재소방학회:학술대회논문집
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• 한국화재소방학회 1994년도 4/4분기 학술 심포지움 발표 논문집
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• pp.10-19
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• 1994

• 미생물학회지
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• 제41권2호
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• pp.117-124
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• 2005

Rotifer와 병든 넙치 자어로부터 분리된 2개의 균주는 표현형적인 특성 확인 결과 Vibrio ichthyoenteri로 확인이 되었다. V. ichthyoenteri를 검출하기 위래 고감도 PCR 방법 개발을 하기 위래 V. ichthyoenteri 16S-23S rRNA intergenic spacer region(ISR)을 분석하였고, V. ichthyoenteri중 특이적 primer를 개발하였다. V. ichthyoenteri 의 ISR를 분석한 결과 1개의 다형성 ISR type서열을 포함하고 있었다. ISR서열은 길이는 348bp이며 tRNA gene을 가지고 있지 않았다. 이 서열을 가지고 이미 알려진 다른 Vibrio 종의 ISR 서열과 mutiple alignment를 수행한 결과 여러 영역에서 높은 가변성을 나타내어 가변 부위를 표적으로 하여 V. ichthyoenteri를 검출하기 위한 종 특이적 primer를 제작하였다. 제작된 primer의 특이성을 확인하기 위해 Vibrio 표준균주 19종의 genomic DNA와 분리균주 18 group에 genomic DNA 그리고 V. ichthyoenteri와 가장 유사한 서열을 가지고 있다고 알려진 Vibrio 종의 genomic DNA를 가지고 시험하였다. 그 결과 본 연구에서 제작된 종 특이적 primer를가지고 PCR 반응을 하면 V. ichthyoenteri를 검출 할 수가 있다.