• 한국지능시스템학회:학술대회논문집
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• 한국퍼지및지능시스템학회 2003년도 춘계 학술대회 학술발표 논문집
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• pp.29-32
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• 2003

We introduce the subcategories IRe $l_{PR}$ (H), IRe $l_{PO}$ (H) and IRe $l_{E}$(H) of IRe $l_{R}$(H) and study their structures in a viewpoint of the topological universe introduced by L.D.Nel. In particular, the category IRe $l_{R}$(H)(resp. IRe $l_{P}$(H) and IRe $l_{E}$(H)) is a topological universe eve, Set. Moreover, we show that IRe $l_{E}$(H) has exponential objects.ial objects.

• Journal of Veterinary Science
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• 제25권2호
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• pp.21.1-21.15
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• 2024

Background: Peste des petits ruminants (PPR) is a contagious and fatal disease of sheep and goats. PPR virus (PPRV) infection induces endoplasmic reticulum (ER) stress-mediated unfolded protein response (UPR). The activation of UPR signaling pathways and their impact on apoptosis and virus replication remains controversial. Objectives: To investigate the role of PPRV-induced ER stress and the IRE1-XBP1 and IRE1-JNK pathways and their impact on apoptosis and virus replication. Methods: The cell viability and virus replication were assessed by 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide assay, immunofluorescence assay, and Western blot. The expression of ER stress biomarker GRP78, IRE1, and its downstream molecules, PPRV-N protein, and apoptosis-related proteins was detected by Western blot and quantitative reverse transcription-polymerase chain reaction, respectively. 4-Phenylbutyric acid (4-PBA) and STF-083010 were respectively used to inhibit ER stress and IRE1 signaling pathway. Results: The expression of GRP78, IRE1α, p-IRE1α, XBP1s, JNK, p-JNK, caspase-3, caspase-9, Bax and PPRV-N were significantly up-regulated in PPRV-infected cells, the expression of Bcl-2 was significantly down-regulated. Due to 4-PBA treatment, the expression of GRP78, p-IRE1α, XBP1s, p-JNK, caspase-3, caspase-9, Bax, and PPRV-N were significantly downregulated, the expression of Bcl-2 was significantly up-regulated. Moreover, in PPRV-infected cells, the expression of p-IRE1α, p-JNK, Bax, and PPRV-N was significantly decreased, and the expression of Bcl-2 was increased in the presence of STF-083010. Conclusions: PPRV infection induces ER stress and IRE1 activation, resulting in apoptosis and enhancement of virus replication through IRE1-XBP1s and IRE1-JNK pathways.

• Journal of Applied Biological Chemistry
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• 제42권2호
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• pp.62-65
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• 1999

Iron regulatory proteins (IRPs) 1 and 2 bind with equally high affinity to specific RNA stem-loop sequences known as iron-responsive elements (IRE) which mediate the post-transcriptional regulation of many genes of iron metabolism. To study putative IRE-like sequences in RNA transcripts using the IRP-IRE interaction, Eight known genes from database were selected and the RNA binding activity of IRE-like sequences were compared to IRP-2. Among them, the IRE-like sequence in 3'-untranslational region (UTR) of divalent ration transporter-1 (DCT-1) shows a significant RNA binding affinity. This finding predicts that IRE consensus sequence present within 3'-UTR of DCT-1 might confer the regulation by IRP-2.

• 대한본초학회지
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• 제29권6호
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• pp.27-33
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• 2014

Objectives : The aims of this study were to exploring the therapeutic effect of Imperatae Rhizoma Extract(IRE) on Asthma. Methods : To investigate biological modulation activities of IRE, we conducted the cell-based assay whether IRE could regulate T helper 2 cells activity with EL4 T cells and mouse splenocytes, and followed animal study to conform the efficacy of their therapeutic potential on OVA-induced asthmatic mouse. Results : In cell study, IRE suppress the nuclear translocation of GATA binding protein-3 protein in phorbol 12-myristate 13-acetate/Ionomycin-stimulated EL4 T cells and Interleukine(IL)-4, IL-5 and IL-13 production in splenocytes at concentration dependent manner. In animal study, IRE-treated groups both 100mg/ml and 200mg/ml improve airway hypersensitibility reaction(AHR) response to methacholine about 30% and 40% with positive control group. Peritoneal blood analysis reveal that eosinophil number and ovalbumin-specific IgE is reduced by IRE treatment. Cell number of eosinophil is also reduced in bronchoalveolar lavage of IRE group like to peritoneal cell and real time-polymerase chain reaction data show that expression levels of IL-4, IL-5 and IL-13 were down regulated in lung tissue. Finally, histological analysis indicate that IRE protect the bronchial tissue damages through the accumulation of inflammatory cells and collagen, and these effect may be cause by interfering Th2 cells activity. Conclusions : Our data represent that IRE potentiates therapeutic activities to the allergic diseases such as asthma by regulating Th2 cells differentiation.

• 한국소음진동공학회:학술대회논문집
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• 한국소음진동공학회 2013년도 춘계학술대회 논문집
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• pp.401-406
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• 2013

This paper presents experimental evaluation of IRE (isolation roller bearing) seismic isolation device. From the combination of base isolation on the IRE system displacement response spectrum and acceleration response spectrum, the compressive strength and the coefficient of friction experiments. Also the IRE system is evaluated by environment test according to KS standards. Both the resonance and seismic experiments using a combination of the IRE and Natural Rubber Bearing (NRB) are performed in order to analyze the seismic isolation of the IRE system dynamic characteristics. For the given load and exciting frequency, the resonant frequency becomes lower, but the resonant magnification remains to be same. However, it is shown that when we consider the IRE only, the vibration on the table with the horizontal movement and the independent horizontal displacement due to the rolling motion of the plate and roller are significantly reduced. This result verifies that the proposed optimal design method of the IRE system is very effective.

• 생명과학회지
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• 제24권10호
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• pp.1039-1045
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• 2014

본 연구의 목적은 마우스 레이디히 세포인 mLTC-1 세포에 사람 융모성 성선자극호르몬인 hCG를 처리하여 유도되는 소포체 스트레스가 IRE1/XBP1 경로를 통하는지 분석하는 것 이다. 이전 연구에서 hCG처리에 의해 레이디히 세포는 소포체 스트레스 매개의 세포자멸사가 유도될 뿐만 아니라 ATF6경로를 조절함으로써 UPR이 성호르몬 합성효소의 발현에 중요한 역할을 하는 것을 증명하였다. UPR 경로는 또한 IRE1/XBP1 경로를 통하여 조절되는 것이 알려져 있지만 레이디히 세포에서 hCG에 의한 소포체 스트레스에 의해 IRE1/XBP1 경로의 활성화가 유도되는지에 대해서는 거의 알려져 있지 않다. mLTC-1세포에서 hCG 처리 후 IRE1/XBP1경로의 활성을 조사하기 위하여, 인산화된 IRE1 단백질 확인하기 위한 western blot, XBP1 mRNA splicing 확인하기 위한 RT-PCR을 수행하였다. 또한 우리는 IRE의 활성을 관찰하기 위하여 소포체 스트레스-활성 표지자(ERAI) construct를 이용하고 이를 형광현미경과 flow cytometry를 이용하여 분석하였다. 결과적으로, hCG 처리에 의해 인산화된 IRE1 단백질의 발현 수준이 두드러지게 증가하였다. F-XBP1-venus/F-$XBP1{\Delta}DBD$-venus가 도입된 mLTC-1 세포에서, hCG 처리에 의해 녹색 형광을 띄는 세포들이 유도되었고 각각 핵/세포질에서 발현하는 것을 확인할 수 있었다. 게다가 hCG 처리 후에 XBP1 mRNA의 splicing 또한 상당히 증가되는 것을 확인 할 수 있었다. 이 결과들을 통하여 종합해 볼 때 레이디히 세포에서 hCG 처리에 의해 유도되는 소포체 스트레스가 IRE1/XBP1 경로의 활성을 유발하는 것을 확인 할 수 있었다.

• Applied Biological Chemistry
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• 제41권3호
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• pp.224-227
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• 1998

철의 대사과정에 관여하는 ferritin 단백질의 발현은 ferritin transcript의 5'-untranslated region에 위치한 iron-responsive element (IRE)와 철 농도 조절 단백질의 결합에 의해 조절된다. 이러한 ferritin의 생성에 관여하는 구조적인 요소를 밝히기 위해, RNA 이차구조인 IRE의 bulge 부분을 다른 염기로 변환시켜 철 농도 조절단백질에 의한 RNA 결합력과 ferritin 단백질의 생성의 저해정도를 비교 측정하였다. 측정된 결과로부터 IRE의 bulge 부분의 시토신 염기배열만이 RNA 이차구조의 형성에 중요한 작용을 하여 ferritin 합성을 조절할 수 있는 것을 보였다.

• 식품보건융합연구
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• 제5권2호
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• pp.27-34
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• 2019

RNases plays a pivotal role in biological system and different RNases are known for their various functions like angiogenesis, immunological response, antiviral, antitumour activity and apoptosis. In which anti tumour activity of RNase is proved to improve genome stability in normal cells up to some extent. RNases like RNase L shows antiviral and antitumour activities against virus infected cells and cancer cells through 2'-5' oligo adenylate pathway and induces RNaseL dependent apoptosis where as RNase A modulates various proliferative pathways like MAP kinase, JNK, TGF-${\beta}$ and activates apoptosis in cancer cells and promotes immunological response through processing of Ags. IRE1 RNase acts as both tumour suppressor gene and oncogene in normal and cancer cells and involved in both antitumour and tumorigenic activities. RNase III upregulates miRNA in cancer cells there by acting via posttranscriptional level and proven to be effective against colorectal adeno carcinoma. In addition to this IRE1 RNase is a double edged sword through RIDD pathway in ER (18). To some of the cancers expressing c-myc IRE1 acts as tumour suppressor where as in cancers where myc is downregulated IRE1 acts as tumour provoking through RIDD pathway (18). Thus RNases play vital role in regulating the genome stability.

• 한국정보처리학회논문지
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• 제5권3호
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• pp.662-670
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• 1998

심볼레벨에서 개발된 1세대 전문가시스템의 문제점을 해결하기 위하여 최근 지식레벨에서 2세대 전문가시스템을 개발하기 위한 여러 가지 방법들이 제안되었으나, 대개 개념적인 모델링 기법을 제시하는 수준에 머무르고 있다. 본 논문에서는 이러한 개념적인 모델링 기법이 실용 가능한 개발기법이 될 수 있도록 기존의 연구 내용을 수정, 보완한 태스크 객체 모델링(TOM) 기법을 제시하였다. 이 기법에서 태스크 객체(TO)는 자신의 목표와 수행 조건, 실행할 행위 지식 등을 포함하는 하나의 지식 단위로 정의하고, TO의 구조적, 동적, 기능적 측면을 쉽게 도식화할 수 있는 태스크 객체 다이어그램(TOD)을 정의하였다. 또한, 각 TO의 행위 지식을 표현하는 기본 단위로서 추론유형(Inference Type)들을 정의하였다. 본 논문에서 제안한 TOM 기법이 단순히 개념적인 모델링 기법이 아니라 실용적인 2세대 전문가시스템 개발기법으로 활용될 수 있도록 TOD 에디터와 TO 에디터를 개발하고, TO의 상태 변화에 기반을 둔 TO 처리 알고리즘을 개발하였다. 또한, 정의된 추론유형들이 대표적인 전문가시스템 개발도구인 IRE(Intelligent Rules element)의 메소드로 자동 변환 될 수 있도록, 각각의 추론유형과 IRE의 메소드를 1: 1로 대응시킨 추론 유형 라이브러리를 구축하였다.

• 한국지구과학회지
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• 제31권5호
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• pp.521-530
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• 2010

This study aimed to explore how to characterize the earth science inquiry in schools in terms of science teaching interaction and constructivist teaching practice. The constructivist teaching practices were analyzed with Reformed Teaching Observation Protocol (RTOP) in three aspects including (1) student oriented class implementation, (2) subject knowledge and representation, and (3) classroom communication. Fourteen earth science classes were observed and scored with RTOP. The class was evaluated to be transitional stage in terms of constructivist teaching, e.g., moving toward student-centered teaching practice. Especially, Korean teachers tend to lean their classes more on propositional knowledge than procedural knowledge. To interpret science teaching interactions, an earth science teacher with a RTOP top rank was selected. Her class was then videotaped for detailed analysis. I adopted the analytical framework of communicative approaches and discourse patterns among the five aspects of interactions presented by Mortimer and Scott (2003). It was found that this earth science teacher used more authoritative patterns than the dialogic. In addition, she used IRE discourse pattern more frequently. Interestingly, teachers interacted with their students more frequently in the form of repeated (or IRE chain pattern), that is IRFRF (teacher initiation-student response-teacher feedback-student response-teacher feedback) in the context of dialogic communicative approaches, while simple IRE occurred in an authoritative approach. In earth science classrooms, typical interaction may well be constructed in the form of IRFRF chains to allow students free conjectures and abduction.