• Journal of Korean Society of Forest Science
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• v.103 no.2
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• pp.240-247
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• 2014

This study was conducted as a restoration research in a mountain stream of hydrologic cycle system, which is a type of microsites purposely changing vegetation. The status of vegetation in the three experimental sites, water purification site, small dammed pole site, and aquatic plant restoration site, and one control site within the area of the mountain forest stream were investigated in three different periods, namely before sites restoration, year of sites restoration, and year after sites restoration. After one year of restoration, number of vegetation was increased in the small dammed pool and control site respectively. Vegetation coverage ratio of Zizania latifolia was increased at the water purification area. The effects of habitats restoration appeared to be good a year after the restoration of the experimental sites, in terms of families and species composition of the introduced vegetation, and stream flow. Therefore, the results of the study strongly suggest that fairly effective ways to restore and reproduce degrading mountain hydroecological habitats are by way of forming pool sites and small dams in intermittent mountain streams and re-vegetating with selected plants.

• Korean Journal of Medicinal Crop Science
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• v.15 no.2
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• pp.95-99
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• 2007

The cost of conventional cultivation of ginseng (Panax ginseng C.A. Meyer) is very expensive, because shadow condition should be maintained during cultivation periods owing to inherently weak plant for high-temperature. Therefore, application of plant biotechnology may be possible to overcome these difficulties caused by conventional breeding of ginseng. Transgenic plants were produced via Agrobacterium tumefaciens Gv3101, both carrying the binary plasmid pBI121 mLPISO with nptII and Iso (isoprene synthase) gene. Integration of the transgenes into the P. ginseng nuclear genome was confirmed by PCR analysis using nptII primers and Iso primers. RT-PCR result also demonstrated the foreign isoprene synthase gene in three transgenic plant lines (T1, T3, and T5) which was expressed at the transcriptional level. When whole plants of transgenic ginseng were exposed to high temperature at $46^{\circ}C$ for 1 h, a non-transformed plant was wilted from heat shock, whereas a transgenic plant appeared to remain healthy. We suggest that the introduction of exogenous isoprene synthase is considered as alternative methods far generating thermotolerance ginseng.

• Korean Journal of Medicinal Crop Science
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• v.13 no.1
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• pp.57-62
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• 2005

Salt-tolerant transgenic Panax ginseng plants were produced by introducing the SAL1 geue (3'(2'), 5'-bis-phosphate nucleotidase) that confers tolerance to the salts through Agrobacterium tumefaciens co-cultivation. Cotyledon explants of immature ginseng zygotic embryos cultured on Murashige and Skoog medium lacking growth regulators formed somatic embryos directly with below 10%, but the 74% tranformation rate were observed at the treatment of phytohormone with 1.0 mg/l 2,4-D and 0.5 mg/l kinetin. Somatic embryos were initially cultured on MS medium supplemented with 250 mg/l cefotaxime for 3 weeks and subsequently subcultured five times to a medium containing 100 mg/l kanamycin and 250 mg/l cefotaxime. Upon development into the cotyledonary stage, these somatic embryos were transferred to on the medium containing 50 mg/l kanamycin and 10 mg/l gibberellic acid to induce germination and strong selection. Integration of the transgene into the plants was confirmed by polymerase chain reaction with specific primers. The ginseng transformants with well-developed shoots and roots were successfully acclimatized in a greenhouse when they were planted in soil.

• Proceedings of the Korean Society for Agricultural Machinery Conference
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• 1996.06c
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• pp.692-700
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• 1996

The objective of this study is to develop a systematic purification plant using the metabolism of aerobic microorganisms. This system is subsequently aerated and continuously removes suspended solids and settling sludges caused by aerating pressure at the bottom of a lower pipe (i.e., Continuous Removal of Suspended solids and Settling sludges, CRSS). The CRSS plants are brought out by introducing fine air bubbles into the liquid phase of a lower pipe in the bio-reactor. These plant uses aeration pipe, with multiple inlets to sweep the floor of bio-reactor tank, instead of the conventional scraper mechanisms. The principal advantage of this system is that it can continuously remove very small or light particles that settles completely within a short time. Once the particles have been floated to the surface, they can be moved into the pipe and collected in the settling tank by sequently aerated pressure. The experimental results shows that about 99.0% of the biochemical oxygen demand(BOD), 99.3% of the suspended solid(SS), 92.3% of the total nitrogen(T-N), 99.0% of the turbidity(TU), 100% of the total coliform(TC)and ammonia was respectively removed during aerobic digestion for 9 days. These result indicates that the CRS S plants are very effective for reduction and deodorization of swine wastewater contaminants, and the efflux from CRS S can either be discharged in the river or used as nutrient solution of formulation for plant growth factories. The developed CRSS plant proved to be flexible and it can simply be adapted to any type of biological waste treatment problem.roblem.

• Journal of People, Plants, and Environment
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• v.22 no.6
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• pp.641-651
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• 2019

The purpose of this study is to derive institutional improvement methods for promoting the Damaged Area Restoration Project in greenbelts. The current status of greenbelts in Gyeonggi-do, where greenbelts are extensively distributed was analyzed, and the relevant laws and regulations were reviewed to suggest measures to promote the restoration project. The area of damaged areas within greenbelts in Gyeonggi-do was 6,121,024 m², accounting for about 0.52% of the total area of greenbelts, and more than 80% was found to be located in Namyangju (55.49%), Hanam (16.48%), and Siheung (8.68%). Various measures to improve the policy were examined as follows: reducing the minimum size of the restoration project area; adjusting baseline of recognizing range of damaged areas; introducing the right of claim for land sale; allowing long-term unexecuted urban parks to be replaced as alternative sites for parks and green spaces; simplifying administrative procedures; and allowing public participation. All of them are expected to promote the restoration project within greenbelts. In results, when the minimum size of area for the restoration project was reduced from 10,000 m² into 5,000 m², 3,000 m² and 2,000 m², the ratio of the number of combinable lots to the total number of lots increased from 4.4% to 18.8%, 38.8%, and 55.9% respectively in Namyangju. Morever, when the recognizable ranges of the restoration project were extended to the structures obtaining building permit as of March 30, 2016 and obtaining use approvals before December, 2017, the number of applicable lots increased by 5.1% and 9.2% respectively.

• Journal of Plant Biotechnology
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• v.36 no.1
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• pp.87-95
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• 2009

The recombinant DNAs, pGBF, pGTF, and pZ4F, using soybean ferritin gene have constructed with the promoters derived from seed proteins, glutelin, globulin, and zein. The recombinant ferritin genes were transformed into rice plant by Agrobacterium-mediated transformation. Iron contents and agronomic traits have been evaluated in the transgenic progenies. The embryogenic calli survived from second selection medium were regenerated at the rates of 19.2% with pGBF, 15.0% with pGTF, and 18.4% with pZ4F in Donganbyeo and 6.7% with pGBF, 11.7% with pGTF, and 3.4% with pZ4F in Hwashinbyeo. The introduction of ferritin gene in putative transgenic rice plants was confirmed by PCR and Southern blot analysis and also the expression of ferritin gene was identified by Northern blot and Western blot analysis. The iron accumulation in transgenic rice grains of the transgenic rice plant, T1-2, with zein promoter and ferritin gene contained 171.4 ppm showing 6.4 times higher than 26.7 ppm of Hwashinbyeo seed as wild type rice, but the transgenic plants with globulin and glutelin showed a bit higher iron contents with a range from 2.1 to 3.0 times compare to wild type grain. The growth responses of transgenic plants showed the large variances in plant height and number of tillers. However, there were some transgenic plants having similar phenotype to wild type plants. In the T1 generation of transgenic plants, plant height, culm length, panicle length, and number of tillers were similar to those of wild type plants, but ripened grain ratio ranged from 53.3% to 82.2% with relatively high variation. The transgenic rice plants would be useful for developing rice varieties with high iron content in rice grains.

• Transactions of the Korean hydrogen and new energy society
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• v.31 no.1
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• pp.122-131
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• 2020

To reduce emissions from coal-fired power plants, researchers focusing on coal and biomass co-firing technology. Biomass, with its carbon-neutral nature and lower quantities of nitrogen and sulfur compared with coals, has a positive impact on coal-fired power generation. Many studies on the combustion of biomass have been conducted, but the study on the combustion characteristics of biomass char is limited. FERPM predicts char combustion characteristics with high accuracy by introducing experimental data-based parameters of biomass char and has not yet been applied in numerical simulation. In this study, FERPM is numerically applied to char combustion of wood pellets representing wood-based biomass and the combustion characteristics are compared with the kinetic/diffusion limited model, intrinsic model, and diffusion limited model.

• Nuclear Engineering and Technology
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• v.31 no.2
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• pp.192-201
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• 1999

This paper proposes an improved signal processing strategy for accurate feedwater flowrate estimation in nuclear power plants. It is generally known that ∼2% thermal power errors occur due to fouling Phenomena in feedwater flowmeters. In the strategy Proposed, the noises included in feedwater flowrate signal are classified into rapidly varying noises and gradually varying noises according to the characteristics in a frequency domain. The estimation precision is enhanced by introducing a low pass filter with the wavelet analysis against rapidly varying noises, and an autoassociative neural network which takes charge of the correction of only gradually varying noises. The modified multivariate stratification sampling using the concept of time stratification and MAXIMIN criteria is developed to overcome the shortcoming of a general random sampling. In addition the multi-stage robust training method is developed to increase the quality and reliability of training signals. Some validations using the simulated data from a micro-simulator were carried out. In the validation tests, the proposed methodology removed both rapidly varying noises and gradually varying noises respectively in each de-noising step, and 5.54% root mean square errors of initial noisy signals were decreased to 0.674% after de-noising. These results indicate that it is possible to estimate the reactor thermal power more elaborately by adopting this strategy.

• Journal of the Earthquake Engineering Society of Korea
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• v.18 no.6
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• pp.291-299
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• 2014

There has been an increasing demand for introducing a base isolation system to secure the seismic safety of a nuclear power plant. However, the design criteria and the safety assessment methodology of a base isolated nuclear facility are still being developed. A performance based design concept for the base isolation system needs to be added to the general seismic design procedures. For the base isolation system, the displacement responses of isolators excited by the extended design basis earthquake are important as well as the design displacement. The possible displacement response by the extended design basis earthquake should be limited less than the failure displacement of the isolator. The failure of isolators were investigated by an experimental test to define the ultimate strain level of rubber bearings. The uncertainty analysis, considering the variations of the mechanical properties of isolators and input ground motions, was performed to estimate the probabilistic distribution of the isolator displacement. The relationship of the displacement response by each ground motion level was compared in view of a period elongation and a reduction of damping. Finally, several examples of isolator parameters are calculated and the considerations for an acceptable isolation design is discussed.

• Journal of Ginseng Research
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• v.34 no.2
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• pp.98-103
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• 2010

The medicinal plant Panax ginseng (P. ginseng) contains various phytosterols and bioactive triterpene saponins (ginsenosides). Squalene synthase catalyzes the first committed step in ginsenoside biosynthesis. Transgenic plants of P. ginseng were generated by introducing the squalene synthase gene derived from P. ginseng. Adventitious roots of the transgenic ginseng grew best in B5 medium, and 2 g of inoculum secured an optimal growth rate. Two phytohormones, indolebutyric acid and 1-naphtalene acetic acid, increased root growth and decreased ginsenoside production. Treatment with two selected elicitors, chitosan and jasmonic acid, and a precursor of the isoprenoid pathway, mevalonic acid, enhanced ginsenoside production and retarded ginseng growth rate.