• Title/Summary/Keyword: IMP-1

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Production of 5-IMP by Auxotroph of Brevibacterium ammoniagenes (Brevibacterium ammoniagenes의 영양구성 변리주에 의한 5 -IMP 생성)

  • 이별나
    • The Korean Journal of Food And Nutrition
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    • v.1 no.2
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    • pp.37-42
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    • 1988
  • In attempts to obtain IMP Producting strains, Brevibacterium ammoniagenes ATCC 6872 was treated wilts N.1.6. Adenine-guanine requiring mutants were obtained from Brevibacterium ammoniagenes ATCC 6872, and then a strain of them was selected for production of IMP and named Brevibacterium ammoniagenes No.9(ade', gu'). The production of IMP by Brevibacterium ammoniagenes nuts No.9 was about 3mg/ml for 4 day of culture. The optimal concentration of adenine and guanine was 150mg/mg.

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Effects of Dietary Nucleotide Supplementation on the Growth Performance, Feed Utilization, Hematological Parameters and Innate Immunity in Red Seabream Pagrus major (사료 내 Nucleotides 첨가가 참돔P(agrus major)의 성장, 사료효율, 혈액성상 및 비특이적 면역반응에 미치는 영향)

  • Song, Jin-Woo;Lee, Kyeong-Jun
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.46 no.6
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    • pp.785-792
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    • 2013
  • Two studies were conducted to investigate the effects of dietary nucleotides (NT) on the growth performance, hematological parameters, and innate immune responses of red seabream Pagrus major. In Expt I, six experimental diets were formulated: a control, four that contained each NT at a level of 0.15% (inosine monophosphate, IMP; adenosine monophosphate, AMP; guanosine monophosphate, GMP; and uridine monophosphate, UMP), and one with a 1:1:1:1 mixture of NTs (IMP, AMP, GMP, and UMP). In Expt II, five experimental diets were formulated that contained 0, 0.1, 0.2, 0.4, and 1.0% IMP (commercial product). Triplicate groups of juvenile (initial body weight 33.1 g) and growing (initial body weight 120 g) red seabream were fed one of the experimental diets to apparent satiation for 8 weeks in Expt I and 12 weeks in Expt II. In Expt I, fish fed diets with NT had higher growth performance than the control group. The nitroblue tetrazolium and lysozyme activities were higher in fish fed the mixed-NT diet, and lowest in the control group. In Expt II, the final body weight and feed utilization of fish fed the 0.1% IMP diet were significantly higher than those of fish fed the control or 1.0% IMP diets. Diet palatability was improved significantly when 0.1% IMP was added. The lysozyme activity was higher in fish fed diets with 0.4-1.0% than in the control group. These results suggest that supplementation of 0.15% IMP and Mixed-NTs in diet can enhance the growth and immune responses in juvenile red seabream. The optimum IMP level appears to be 0.2% in practical feed formulation for growing red seabream.

Effects of Dietary Supplementation with Nucleotide on Growth Performance, Feed Utilization, and Non-Specific Immune Responses of Nile Tilapia Oreochromis niloticus (사료 내 nucleotide 첨가가 틸라피아(Oreochromis niloticus)의 성장, 사료효율 및 비특이적 면역력에 미치는 영향)

  • Song, Jin-Woo;Lim, Se-Jin;Oh, Dae-Han;Cha, Ji-Hoon;Lee, Kyeong-Jun
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.45 no.6
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    • pp.648-653
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    • 2012
  • The present study examined the effects of dietary supplementation with nucleotide (inosine monophosphate product, IMP) on the growth performance, feed utilization, and non-specific immune responses of juvenile tilapia Oreochromis niloticus. Triplicate groups of tilapia (initial body weight, $7.4{\pm}0.04$ g) were fed experimental diets containing 0%, 0.05%, 0.1%, and 0.2% IMP. Fish were fed six times a day until apparent satiation for 13 weeks. At the end of the feeding trial, final body weight and food utilization of fish fed 0.1% IMP were significantly higher than those of fish fed the control diet. Results of hematological parameters were not affected by dietary IMP. However, blood protein level was significantly higher in the 0.05% treatment, as compared to that of the control and 0.2% IMP diets. Myeloperoxidase activity was higher in fish fed 0.1% IMP than in fish fed the control and 0.2% IMP diets. These results suggest that dietary supplementation with IMP can enhance the growth performance, feed utilization, and innate immune response of juvenile tilapia. The optimal IMP supplementation level appears to be 0.1% in practical feed formulations for tilapia.

Molecular Epidemiology of Metallo-β-lactamase Producing Pseudomonas aeruginosa Clinical Isolates (임상에서 분리된 Metallo-β-lactamase 생성 Pseudomonas aeruginosa의 분자역학)

  • Choi, Myung-Won
    • Journal of Life Science
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    • v.22 no.9
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    • pp.1268-1276
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    • 2012
  • The emergence and dissemination of carbapenem-resistant bacteria have resulted in limitations of antibiotic treatment and potential outbreaks of metallo-${\beta}$-lactamase (MBL) producing Pseudomonas aeruginosa resistant to carbapenems. In this study, we conducted molecular characterization of the MBL genes of the ${\beta}$-lactam drug-resistant P. aeruginosa and prepared basic data for treatment and prevention of proliferation of antimicrobial-resistant bacterial infections. Forty-two P. aeruginosa isolates of 254 were resistant to imipenem or meropenem. Among the 42 isolates, 28 isolates were positive for the Hodge test, and 23 isolates were positive for the EDTA-disk synergy test (EDST). MBLs were detected in 59.5% (25/42) of P. aeruginosa isolates. Eight isolates harbored $bla_{IMP-6}$, whereas 17 isolates harbored $bla_{VIM-2}$. The $bla_{IMP-6}$ gene was in a class 1 integron containing five gene cassettes: $bla_{IMP-6}$, qac, aacA4, $bla_{OXA-1}$, and aadA1. Some strains that produce IMP-6 and VIM-2 showed epidemiological relationships. The $bla_{IMP-6}$ gene in carbapenem-resistant P. aeruginosa showed an identical pattern to a gene cassette that was reported at a hospital in Daegu, Korea. Therefore, MBL-producing P. aeruginosa is already endemic in the community. We are concerned that the existence of carbapenem-resistant bacteria containing the blaMBL gene may increase pressure on antibiotic selection when treating infections. We believe that we should select appropriate antibiotics based on the antibiotic susceptibility test and continue the research to prohibit the emergence and spread of antibiotics resistant bacteria.

A Rapid Method for Determination of ATP and Its Related Compounds in Dried Fish and Shellfish Products Using HPLC (HPLC에 의한 시판수산건제품의 ATP분해생성물의 신속정량법)

  • LEE Eung-Ho;KOO Jae-Geun;AHN Chang-Bum;CHA Yong-Jun;OH Kwang-Soo
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.17 no.5
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    • pp.368-372
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    • 1984
  • This paper deals with a rapid method for determination of ATP and its related compounds in fish and shellfish products using high performance liquid chromatography(HPLC). The HPLC used is a HPLC/ALC-224 equiped with UV-spectrophotometer (254 nm) as detector and integrator (Yanagimoto system-1000). The column used is a stainless steel tubing ($30.0\;cm{\times}3.9\;mm\;i.d.$) packed with ${\mu}-Bon-dapak\;C_{18}$. A mixture of $1\%$ triethylamine-phosphoric acid(pH6.5) was used as an eluent and the flow rate of the eluent was controlled at 2 ml/min. For the separation of ATP and its related compounds, a standard mixture of ATP, ADP, AMP, IMP, inosine and hypoxanthine was subjected to HPLC under the above mentioned conditions. Six peaks were obtained with retention times within 20 min, and elution order were hypoxanthine, IMP, inosine, AMP, ADP and ATP. But 5'-IMP and 5'-GMP fractions were not separated by this method. In generally, IMP content in boiled-dried fish and shellfish products purchased from the market was comparatively higher than that of other nucleotides. Especially, boiled-dried big eye herring marked higher value in IMP content than other boiled-dried ones. Hypoxanthine and inosine were major components of ATP-related compounds in dried products and seasoned-dried ones. And IMP content in seasoned-dried products was higher than that of dried ones. This fact is suggested that a part of IMP in seasoned-dried ones was derived from flavoring matter (MSG, 5'-IMP and 5'-GMP) which is added during the seasoning treatment.

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Factors Affecting the Inosine Monophosphate Content of Muscles in Taihe Silkies Chickens

  • Chen, G.H.;Li, H.F.;Wu, X.S.;Li, B.C.;Xie, K.Z.;Dai, G.J.;Chen, K.W.;Zhang, X.Y.;Wang, K.H.
    • Asian-Australasian Journal of Animal Sciences
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    • v.15 no.9
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    • pp.1359-1363
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    • 2002
  • In this paper, changes in the concentration of inosine monophosphate (IMP) of muscles in Taihe Silkies chickens from 2 to 28 weeks of age were studied. The results showed that: (1) with increasing age, IMP content of muscles decreased continuously. (2) the relationship between body weight and IMP content of musculus pectoralis major was significantly negative (p<0.01), so was body weight and IMP content of musculus peronaeus, and their coefficients of correlation were -0.45, -0.38 respectively; the relationship between IMP contents of musculus pectoralis major and that of musculus peronaeus was more significantly positive (p<0.01), and its coefficient of correlation was 0.59. (3) by multiple regression analysis, changes of IMP content of muscles depended on the weeks of age and body weight. (4) from 2 to 28 weeks of age, the heritabilities of IMP content of musculus pectoralis major were calculated between 0.33-0.48, and those of musculus peronaeus were calculated between 0.51-0.69.

Detection of the Carbapenem Resistance Gene in Gram-negative Rod Bacteria Isolated from Clinical Specimens (임상검체에서 분리된 그람음성막대균으로부터 카바페넴 내성 유전자 검출)

  • Yang, Byoung Seon
    • Korean Journal of Clinical Laboratory Science
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    • v.54 no.3
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    • pp.179-191
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    • 2022
  • Carbapenem-resistant Enterobacteriaceae (CRE) poses an increasing public health threat and has limited treatment options with high associated mortality. Genotypes of carbapenemase that threaten public health (blaKPC, blaNDM, blaIMP, and blaVIM) and blaOXA-48-like genes were detected by phenotypic and molecular diagnosis, and related gene distribution patterns were investigated. Phenotypic testing using the modified Hodge test confirmed positivity in all 41 strains examined, and carbapenemase inhibitory testing using meropenem+phenyl boronic acid or meropenem+EDTA confirmed positivity in 18 and 8 strains, respectively. Polymerase chain reaction revealed the presence of amplification products in 28 strains of blaKPC, 25 strains of blaNDM, 5 strains of blaIMP, 1 strain of blaVIM, and 13 blaOXA-48-like strains. In addition, 7 strains of blaKPC+blaNDM, 1 strain of blaKPC+blaIMP, 1 strain of blaNDM+blaOXA-48-like, 1 strain of blaNDM+blaVIM, 4 strains of blaKPC+blaNDM+blaIMP, and 4 strains of blaKPC+blaNDM+blaOXA-48-like were identified. Melting curve analysis using real-time PCR was wholly consistent with PCR results. The study shows genetic identification of highly specific CRE by real-time PCR could be used to provide early diagnoses and infection control, improve surveillance, and prevent the transmission of CRE.

A Study on an Efficient Method to Evaluate Intermodulation Product (혼변조적의 효과적인 산출 방식에 관한 연구)

  • 고성찬;황인환;최형진
    • Journal of the Korean Institute of Telematics and Electronics A
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    • v.30A no.9
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    • pp.14-23
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    • 1993
  • In this paper, we present analysis results of various intermodulation products (IMPs) evaluation methods previously proposed for nonlinear systems. The results indicate that out of these methods, Fuenzalida and Simbo's method is the best for efficient evaluation of IMPs. Also, we present a detailed mathematical analysis of IMP and show how to apply the equations related to IMP to actual program implementations with examples. In this paper, we newly introduce three methods to reduce the IMP calculation time and improve the accuracy of the outputs :1) TWTA curve fitting method by LS (Least Square), 2) IMP evaluation technique which is based on a look-up table 3) Gaussian spectral shaping for PSK signal instead of convolution. The IMP evaluation results obtained by the new approaches introduced in this paper resulted in a good match with the published outputs in the open literature and showed improved performance especially in the TWTA curve .

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Identification of Feeding Stimulants for Juvenile Pacific Bluefin Tuna, Thunnus orientalis in Muscle Extract of Horse Mackerel, Trachurus japonicus (참다랑어(Thunnus orientalis) 치어에 있어서 전갱이(Trachrus japonicus) 근육 엑기스 중의 섭이촉진물질 검색)

  • Ji, Seung-Cheol;Takaoka, Osamu;Seoka, Manabu;Kohbara, Jun;Hosokawa, Hidetuyo;Shimeno, Sadao;Jeong, Gwan-Sik;Lee, Si-Woo;Takii, Kenji
    • Journal of Aquaculture
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    • v.20 no.1
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    • pp.19-25
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    • 2007
  • For establishing a basal diet for the Pacific bluefin tuna Thunnus orientalis (PBT), feeding stimulants were initially identified by omission test using the synthetic extract of horse mackerel, Trachurus japonicus. Four feeding trials were conducted using juvenile PBT weighing $9.0{\pm}0.91\;g$ (trial 1, 2 and 3) and $1.6{\pm}0.23\;g$ (trial 4), which were originated from an artificial seedling production. The fish fed the casein diet with each test solution were added at the ratio of 100 g casein diet to 100 g jack mackerel muscle. A complete synthetic extract of jack mackerel containing all 3 fractions, amino acid, nucleotide and organic nitrogenous base, exhibited a comparable feeding stimulant activity compared to that of natural extract. The omission of nucleotide or amino acid fraction showed lower feeding activity, but the omission of other nitrogenous fraction maintained a similar feeding stimulant activity compared to that of the synthetic extract (trial 1). Inosine-5' monophosphate $Na_2$ (IMP) was identified as a major constituent for maintaining feeding activity. The mixture of L-alanine, L-glutamic acid, L-histidine, L-lysine, taurine and IMP induced a similar feeding activity compared to that of the synthetic extract (trial 2 and 3). In trial 4, the highest feeding activity was finally obtained in the mixture of L-histidine, L-glutamine and IMP, followed by the synthetic extract, the mixture of L-lysine, L-alanine and IMP, IMP and the mixture of L-histidine, L-glutamic acid, L-lysine and L-alanine. These results revealed that the mixture of L-histidine, L-glutamic acid and IMP for the proper feeding stimulant of PBT in this study.

Studies on the Fermentative Production of Inosine 5'-monophosphate by Microorganisms. (Part II) Effects of Carbon Source and Purine Base on Inosine 5'-monophosphate Accumulation by a Mutant of Brevibacterium ammoniagenes (미생물에 의한 5'-이노신산의 생산에 관한 연구 (제 2보) Brevibacterium ammoniagenes 이변주에 의한 5'-이노신산의 생성에 미치는 탄소원과 Purine염기의 영향)

  • ;;;;Hiroshi Iizuka
    • Microbiology and Biotechnology Letters
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    • v.9 no.1
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    • pp.45-50
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    • 1981
  • The effect of growth and the carbon sources including the molar ratio of fructose to glucose was studied for the maximization of inosine-5'-monophosphate (5'-IMP) production from Brebibacterium ammoniagenes D-21530. According to experimental results, fructose was more efficient to 5'-IMP accumulation than glucose, while the latter was better for the cell growth than the former. To synchronously use glucose and fructose as carbon source is to optimally control the cell growth and maximum production of 5'-IMP without change of other conditions. The optimal weight percent of fructose to sum of glucose and fructose was 20~40%, and the productivity improvement over the utilization of fructose was about 40%. And also the optimality of purine base such as adenine and guanine were considered. The optimal concentrations of adenine and guanine were near 50㎎/l.

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