• Title/Summary/Keyword: IMP

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Isolation and Characterization of Bacillus subtilis MP56 with Antimicrobial Activity against MDR (Multi Drug Resistant) Strains (다약제내성균에 대한 항균 활성을 가지는 Bacillus subtilis MP56 균주의 분리 및 특성분석)

  • Park, Sungyong;Yoo, Jincheol;Seong, Chinam;Cho, Seungsik
    • Korean Journal of Microbiology
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    • v.49 no.1
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    • pp.90-94
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    • 2013
  • A new Bacillus strain designated as MP56 producing antimicrobial substance has been isolated from the mud flat of Korea. The strain MP56 was found to exhibit broad spectrum of antimicrobial activity against Gram-positive pathogenic microorganisms and MDR (multi drug resistant) strains. The 16S rRNA sequence revealed that the MP56 was closely related to Bacillus subtilis with 99.93% homology. The optimal medium composition for production of antimicrobial substance in the B. subtilis MP56 were 1% mannitol, 1% oat meal, 0.01% $CaCl_2$. Antimicrobial activity of the culture broth against different pathogenic strains was assessed using the antimicrobial spectrum. The result suggests that Bacillus strain MP56 produces high quality antimicrobial substance that might be very useful to control varieties of pathogenic microbial growth.

Studies on the Food of Fresh Water Fish ( I ) - The Chemical Composition of Carp Muscle, Cyprinus Carpio - (담수어(淡水魚)의 식품학적(食品學的) 연구(硏究) (I) - 잉어 육(肉)의 화학성분(化學成分) -)

  • Sung, Nak-Ju;Shim, Ki-Hwan;Lee, Jong-Ho;Lee, Jong-Mi
    • Journal of Nutrition and Health
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    • v.13 no.1
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    • pp.59-64
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    • 1980
  • Carp, Cypinus carpio, has been esteemed as one of the most tasty fresh water fishes in Korea. But little study on its food has been reported. The composition of fatty acids, amino acids, nucleotides and their related compounds as chemical component of carp muscle were analyzed by gas-liauid chromatography, amino acid autoanalyzer and high speed liauid chromatography. The fatty acids of the carp muscle lipid consisted of the large amount of $C_{18}\;:\;2,$ $C_{18}\;:\;1,$ $C_{18}\;:\;3,$ $C_{16}\;:\;0,$ $C_{16}\;:\;1,$ acids, small amount of $C_{16}\;:\;2,$ $C_{18}\;:\;0,$ $C_{18}\;:\;4,$ $C_{14}\;:\;0,$ $C_{14}\;:\;1,$ $C_{12}\;:\;0,$ acids, and $C_{13}\;:\;1,$ $C_{13}\;:\;0,$ $C_{20}\;:\;1,$ $C_{17}\;:\;0,$ $C_{15}\;:\;1,$ $C_{17}\;:\;1,$ $C_{22}\;:\;1$ acids were smaller. In fresh carp, the results showed that inosine $17.25{\mu}mole/g$, hypoxanthine $10.06{\mu}mole/g$ were dominant and the content of GMP, IMP, AMP, UMP, CMP were 1.56, 1.36, 0.92, 0.49, $0.34\:{\mu}mole/g$ on dry base, respectively. Histidine and lysine were dominant amino acids in carp extract, having 49.4% (214.6mg%), 33.9% (147.1mg%) of total free amino acid contents, respectively, but the content of glycine, serine, alanine and glutamic acid were low, and arginine, aspartic acid, threonine, proline, valine, methionine, isoleucine, phenylalanine were detected in trace amount. The content of amino acid composition showed that glutamic acid, aspartic acid, lysine, leucine, alanine, valine and arginine were the most abundant amino acids, while such amino acids as glycine, isoleucine, phenylalanine, threonine, histidine, methionine, serine and tyrosine were low, and proline was detected in trace amount.

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Studies on the Processing of Rapid- and low Salt-Fermented Liquefaction of Anchovy(Engrulis japonica)(III) - Changes in ATP-related compounds, TMAO, TMA, Creatine, and Creatinine during Fermentation - (저식염 속성 멸치 발효액화물 가공에 관한 연구(III) - 숙성 중 ATP관련화합물, TMAO, TMA, creatine 및 creatinine 함량변화 -)

  • Park, Choon-Kyu
    • Journal of the Korean Society of Food Culture
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    • v.17 no.4
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    • pp.482-495
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    • 2002
  • Changes in ATP and related compounds, TMAO, TMA, creatine and creatinine were analyzed to establish the processing conditions for rapid- and low salt-fermented liquefaction of anchovy(Engrulis japonica) extracts during fermentation. Experimental sample A: chopped whole anchovy, adding 20% water, heating at $50^{\circ}C$ for 9 hrs and then adding 10% NaCl. Sample B: chopped whole anchovy, adding 20% water, heating at $50^{\circ}C$ for 9 hrs and then adding 13% NaCl. Sample C: chopped whole anchovy adding 13% NaCl. Sample D: whole anchovy adding 17% NaCl. ATP, ADP, AMP and IMP were broken down during fermentation period, while inosine and hypoxanthine or hypoxanthine were detected in each fermented liquefaction of anchovy. However the amounts of them were varied from collection to collection according to the pretreatment methods. Possibly ATP and their related compounds will not make a great contribution to the umami taste in fermented liquefaction of anchovy. The contents of TMAO were decreased during fermentation period, ranging from 3 to 15 mg/100g in the fermented liquefaction of anchovy after 180 days. The TMA contents were increased slowly during fermentation period, ranging from 60 to 114 mg/100g in the 180 days specimens, however their contents were varied from sample to sample. The contents of creatine and creatinine were increased during early fermentation period, and then they were decreased in the last period. As for distribution of nitrogen in the anchovy extracts, the contribution of creatine and creatinine to the extractive nitrogen was occupying 6.8, 5.7, 4.6 and 5.7% in the experimental sample A, B, C and D, respectively. The contribution of ATP and related compounds to the extractive nitrogen was occupying 2.1, 2.4, 2.2 and 2.7% in the experimental sample A, B, C and D, respectively. The contribution of TMAO and TMA to the extractive nitrogen was very low as they are occupying $0.7{\sim}1.2%$ in the four experimental samples.

Comparison of the Taste Compounds of Loach Differing in the Local, Growing Condition and Season (산지, 성장조건 및 계절별 미꾸라지의 정미성분 비교)

  • Kim, Hee-Yun;Shin, Jae-Wook;Sim, Kyu-Chang;Park, Hee-Ok;Jang, Young-Mi;Kim, Hyun-Sook;Hu, Jong-Wha
    • Korean Journal of Food Science and Technology
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    • v.32 no.4
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    • pp.772-787
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    • 2000
  • In this study, evaluation on the loaches from the various sources was carried out in respect of their taste compounds. The samples were classified by local, season and growing condition and evaluated in terms of composition, the refuse, contents of nucleotides and their related compounds, total and free amino acid, organic acid and fatty acid. Evaluation on the compositions revealed the fact that, in terms of the local, the imported loach contain a low level of fat and high level of ash, in comparison with Korean loach; in terms of growing condition, cultured loach contains a high level of fat and low level of moisture, in comparison with wild loach; and in terms of the season, the summer loach contains less moisture than the fall loath; but as for the contents of other component, the summer loach was superior. As for the refuse, the imported loach had a greater refuse than the Korean loach. In the growing condition, the wild loach had lower value than the cultured loach. There was no substantial difference in each season. Also nucleotide and these related compounds were detected in all of the samples, while ATP was not found. The content levels of such detected compound did not show a substantial difference in each condition. However, generally, the imported loach was poor in such compound and all of the samples were high in IMP. The samples were high in total amino acids. The local and growing conditions showed no substantial difference, while in the season, the summer loach had a higher content of total and essential amino acid than the fall loach. As for the contents of free amino acids, similar distribution of the composition was shown in each sample, even though there was little difference in their constituents. Also, total content of free amino acid varied with the conditions of the samples. As for total content of organic acid and the distribution of the composition, there was a little difference between each condition. In the season and growing condition, the summer loach was high in organic acid. As for the distribution of the fatty acid compositions, there was no substantial difference between each condition and each sample. In the case of the wild loach, the summer loach was high in ${\omega}-3$ polyunsaturated fatty acid and the fall loach was high in essential fatty acid. Similarly, in the case of the imported loach, the summer loach was high in ${\omega}-3$ polyunsaturated fatty acid and the fall loach was high in essential fatty acid.

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The Porcine FoxO1, FoxO3a and FoxO4 Genes: Cloning, Mapping, Expression and Association Analysis with Meat Production Traits

  • Yu, Jing;Zhou, Quan-Yong;Zhu, Meng-Jin;Li, Chang-Chun;Liu, Bang;Fan, Bin;Zhao, Shu-Hong
    • Asian-Australasian Journal of Animal Sciences
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    • v.20 no.5
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    • pp.627-632
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    • 2007
  • FoxO1, FoxO3a and FoxO4 belong to the FoxO gene family, which play important roles in the PI3K/PKB pathway. In this study, we cloned the porcine FoxO1, FoxO3a and FoxO4 sequences and assigned them to SSC11p11-15, SSC1p13 and SSC xq13 using somatic cell hybrid panel (SCHP) and radiation hybrid panel (IMpRH). RT-PCR results showed that these three genes are expressed in multiple tissues. Sequencing of PCR products from different breeds identified a synonymous T/C polymorphism in exon 2 of FoxO3a. This FoxO3a single nucleotide polymorphism (SNP) can be detected by AvaII restriction enzyme. The allele frequencies of this SNP were investigated in Dahuabai, Meishan, Tongcheng, Yushan, Large White, and Duroc pigs. Association of the genotypes with growth and carcass traits showed that different genotypes of FoxO3a were associated with carcass length and backfat thickness between 6th and 7th ribs (BTR) and drip loss (p<0.05).

Comparision of Food Components in the Raw, Cooked Meat and Cooked Meat Extracts of Cookie Shell -2. Nitrogenous compounds and minerals- (새조개 생육과 자숙육 및 자숙액즙의 식품성분 비교 -2. 함질소엑스성분 및 무기성분의 비교-)

  • KIM Kui-Shik;HA Bong-Seok;BAE Tae-Jin;JIN Joo-Hyeon;KIM Hyeon-Ju
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.26 no.2
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    • pp.111-119
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    • 1993
  • The nitrogenous compounds and minerals in the raw and cooked meat of cockle shell were analyzed, and compared with those of cooked meat extracts. In abundant free amino acids, the content of glutamic acid was $129mg\%$ in raw meet, $105mg\%$ in cooked meat, $28mg\%$ in cooked meet extracts, aspartic acid, glycine, arginine, lysine, leucine, and alanine in order. The major components were lysine, arginine and leucine, and the minor components of essential amino acids were proline, tyrosine, serine and cystine. Some of ATP, ADP, AMP, inosine and hypoxanthine were identified in raw and cooked meat, but IMP and inosine were not detected in cooked meat extracts. A slight drop in content of ATP was showed in cooked meat and those had a higher content in inosine and hypoxanthine compared with raw meat. TMA, TMAO and betaine were also checked in all meat products and TMA slightly increased during cooking. Minerals in cooked cockle shell products were phosphorous, potassium, calcium and zinc. The content of phosphorous showed the highest value($16mg\%$ in raw, $185mg\%$ in cooked meat, and $25mg\%$ in extracts).

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Comparison of glucose, lactate, and nucleotide degradation products content of cooked Hanwoo and Australian beef steaks by internal temperature (가열 한우육 및 호주산우육 스테이크에서 심부온도에 따른 glucose, lactate 및 핵산 분해 물질 함량 비교)

  • Kang, Sun-Moon;Kang, Geun-Ho;Seong, Pil-Nam;Kim, Young-Chun;Kim, Jin-Hyoung;Ba, Hoa Van;Jang, Seon-Sik;Cho, Soo-Hyun
    • Korean Journal of Agricultural Science
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    • v.42 no.4
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    • pp.369-374
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    • 2015
  • This study was conducted to compare the glucose, lactate, and nucleotide degradation products content of cooked beef steaks from Korean Hanwoo (quality grade: 1) and Australian cattle (Bos indicus, grain-fed for 100 d) by internal temperature. The loins (M. longissimus dorsi) and top rounds (M. semimembranosus) from two cattle breeds were cut into about 2 cm thickness and then cooked in a $180^{\circ}C$ electronic oven until internal temperature attained to 50, 70, or $90^{\circ}C$. Regardless of internal temperature, glucose content was higher (P<0.05) in cooked loin and top round steaks from Hanwoo compared to those from Australian cattle. Lactate content was shown to be lower (P<0.05) in cooked steaks from Hanwoo than in those from Australian cattle. Lower (P<0.05) hypoxanthine and higher (P<0.05) guanosine 5'-monophosphate, inosine 5'-monophosphate, inosine contents were observed in cooked steaks from Hanwoo. Furthermore, glucose content tended to be decreased by internal temperature but nucleotide degradation products content was not changed by internal temperature. Therefore, these findings suggest that cooked Hanwoo beef steaks had higher flavor precursors related to sweet and umami tastes than cooked Australian beef steaks

Search for Viable Alternative for the Korean Occupational Welfare System at the Crossroad (전환점에 서있는 한국기업복지제도의 한 대안으로서 근로자협조프로그램의 적용성에 관한 연구)

  • Kim, Ui-Myong
    • Korean Journal of Social Welfare
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    • v.36
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    • pp.71-99
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    • 1998
  • There is a general consensus that fate of the differentiated welfare state had fallen into the brink of demise during the 1970s while the integrated welfare state model has emerged instead. Indications are all that such trend spotlights occupational welfare which has been relatively neglected, especially, in the field of social welfare. Nevertheless, the occupational welfare system in Korea faced crisis due to two major incidents. One is the IMP crisis which necessitates massive scale of restructuring of the general fabric of the Korean business and industry. There is little doubt that the economic crisis will surely cut down occupational welfare expenditure substantially. The other is a report released by the Korean Labor Research Institute which argus that there is no correlation between the Korean occupational welfare system and labor productivity. Thorough investigation of the Korean occupational welfare system suggests that current system is so outdated that it doesn't meet needs of the industrial workforce which, in turn, fails to enhance workers productivity. Consequently, a need for new paradigm and system arises and employee assistance program(EAP) emerges as a viable alternative. EAP is thoroughly delianeated of it's definition, motivations, functions, ingredients, historical background as well as cost-effectiveness. But major tasks center around an investigation and an analysis of it's applicability. In-depth analysis was carried out on the issue of it's indispensable natures and stumbling blocks on the way to lay it's root in the Korean industrial environment. It seems harder than the herculean task. However, EAP is not simply an alternative but is rather a pressing imperative for the present occupational welfare system in Korea which stands at the crossroad.

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Effects of Adding Crushed Fresh Sweet Potatoes on the Quality of Rice Straw, Clover and Sweet Potato Stalk Silages (볏짚, 클로버 및 고구마덩굴 Silage 조제(調製)를 위한 생고구마 첨가(添加)의 효과(效果))

  • Kim, Jong Woo;Kim, Yong Kook
    • Korean Journal of Agricultural Science
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    • v.11 no.1
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    • pp.114-119
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    • 1984
  • In order to improve the quality of silages made of rice straw, clover and sweet potato stalk, crushed fresh sweet potatoes(CFSP) were added at various levels to each silage. When the fermentation was completed, the chemical composition, pH and main organic acid contents of each silage were analized. The results obtained were summarized. 1. The moisture content of sweet potato stalk silage was highest among all silages tested. The clover silage showed the highest value in crude protein content. On the other hand, the rice straw silages were high in NFE and crude fiber contents. When the level of CFSP increased, the NFE contents tended to increase and crude fiber decrease. 2. The content of total organic was highest in clover silage. However, the percentage of lactic acid in total acid content was highest in sweet potato silage. The percentage of lactic ac id in total acid content was increased in paralled with the levels of CFSP. 3. The sweet potato stalk silages showed the lowest pH value (4.10-4.20). The highest pH values were 4.75-5.22 in rice straw silage. The addition of CFSP tended to lower the pH. 4. It appears the quality of clover and rice straw silages can be imp roved by adding CFSP at 5 and 10% levels, respectively, on fresh weight basis. The addition of CFSP to the sweet potato stalk silage, however, was not effective in this experiment.

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Growth and Lodging of Rice as Affected by Growth Regulators under Different Midsummer Drainage Times in Puddled-soil Drill Seeding (벼 무논골뿌림 재배시 중간낙수 및 생장조절제 처리가 생육 및 도복에 미치는 영향)

  • 김상수;백남현;이선용;김종호;조동삼
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.40 no.6
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    • pp.697-704
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    • 1995
  • This experiment was carried out to elucidate the effect of the midsummer drainage times and some growth regulators on lodging characters, lodging and yield in puddled-soil drill seeding in rice. Dongjinbyeo, the mid-late maturing rice variety was seeded at May 11 by seeding machine with 4cm of furrow depth. Experimental plots were divided two main treatment without midsummer drain and two time drains (30 and 50 days after seeding), Inabenfide(IBF) was applied 40days before heading(DBH) and IBP was applied at 30DBH, respectively. Culm length was shorten, the wall of N$_4$ was thicken, and the breaking weight was increased at two time drainage and growth regulators applied in order of Inabenfide, IBP, and Control. Lodging wasn't occurred at two time drainage but it was occurred at none drainage in the order of Control, IBP, and Inabenfide applied. Yield was higher at two time drainage compared with none drainage and higher in order of Inabenfide, IBP and Control in none drainage but wasn't significantly different among growth regulators applied in two time drainage. Therefore, two times midsummer soil drying is recommendable management method for puddled -soil drill seeding of rice. Rice, Direct seeding, Puddled-soil, Drill seeding, Midsummer drainage, Growth regulator, Lodging.

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