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Ultrastructural Studies of Vitellogenesis According to Germ Cell Development, and Mating Period and Spawning Activity in Female Rapa Whelk, Rapana venosa (Gastropoda: Muricidae) in the Brackish Water Area of Seomjin River, Korea (한국 섬진강 기수역 암컷 피뿔고둥 Rapana venosa (복족류 : 뿔소라과)의 생식세포 발달에 따른 난황형성과정의 미세구조적 연구 및 교미 시기와 산란 활성)

  • SON, Pal-Won;LEE, Il-Ho;KIM, Sung-Han
    • Journal of Fisheries and Marine Sciences Education
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    • v.27 no.4
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    • pp.1031-1040
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    • 2015
  • Ultrstructural studies of germ cell differentiation and vitellogenesis in the oocytes of the female Rapana venosa in the brackish water area of Seomjin River were investigated by transmission electron microscope observations. In the early vitellogenic oocytes, the Golgi complex and mitochondria were involved in the formation of glycogen particle, lipid droplets, and yolk granules. In the late vitellogenic oocytes, the rough endoplasmic reticulum and multivesicular bodies were involved in the formation of proteid yolk granules in the cytoplasm. However, heterosynthetic vitellogenesis in this species were not observed in vitellogenic oocytes during oogenesis. A mature yolk granule was composed of three components: crystalline core, electron lucent cortex and the limiting membrane. As shown in some large gastropods, vitellogenesis in R. venosa occurred by way of endogenous autosynthesis without heterosythetic vitellogenesis (exogeneous endocytosis), which are found in the oocytes in bivalves. The mating period and spawning activity were related with the increases of seawater temperatures and salinities.

Korean Red Ginseng attenuates anxiety-like behavior during ethanol withdrawal in rats

  • Zhao, ZhengLin;Kim, Young Woo;Wu, YiYan;Zhang, Jie;Lee, Ju-Hee;Li, XiaoHua;Cho, Il Je;Park, Sang Mi;Jung, Dae Hwa;Yang, Chae Ha;Kim, Sang Chan;Zhao, RongJie
    • Journal of Ginseng Research
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    • v.38 no.4
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    • pp.256-263
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    • 2014
  • Background: Korean Red Ginseng (KRG) is known to have antianxiety properties. This study was conducted to investigate the anxiolytic effects of KRG extract (KRGE) during ethanol withdrawal (EW) and the involvement of the mesoamygdaloid dopamine (DA) system in it. Methods: Rats were treated with 3 g/kg/d of ethanol for 28 d, and subjected to 3 d of withdrawal. During EW, KRGE (20 mg/kg/d or 60 mg/kg/d, p.o.) was given to rats once/d for 3 d. Thirty min after the final dose of KRGE, anxiety-like behavior was evaluated in an elevated plus maze (EPM), and plasma corticosterone (CORT) levels were determined by a radioimmunoassay (RIA). In addition, concentrations of DA and 3,4-dihydroxyphenylacetic acid (DOPAC) in the central nucleus of the amygdala (CeA) were also measured by high performance liquid chromatography (HPLC). Results: The EPM test and RIA revealed KRGE inhibited anxiety-like behavior and the over secretion of plasma CORT during EW. Furthermore, the behavioral effect was blocked by a selective DA D2 receptor (D2R) antagonist (eticlopride) but not by a selective DA D1 receptor (D1R) antagonist (SCH23390). HPLC analyses showed KRGE reversed EW-induced decreases of DA and DOPAC in a dose-dependent way. Additionally, Western blotting and real-time polymerase chain reaction (PCR) assays showed that KRGE prevented the EW-induced reductions in tyrosine hydroxylase (TH) protein expression in the CeA and TH mRNA expression in the ventral tegmental area (VTA). Conclusion: These results suggest that KRGE has anxiolytic effects during EW by improving the mesoamygdaloid DA system.

Ammonia Removal by using RBC in Recirculating Aquaculture System (RBC를 이용한 양어장수 암모니아 제거)

  • KIM Byong-Jin;LIM Sung-Il;SUH Kuen-Hack
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.31 no.5
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    • pp.622-630
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    • 1998
  • Rotating Biological Contactor (RBC) was tested for the removal of total ammonia nitrogen (TAN) by using simulated aquaculture system. RBC performance was evaluated by controlling revolution rate of disk and hydraulic residence tile (HRT). The optimum revolution rate of disk was 4 rpm, As HRT of RBC was increased, TAN removal efficiency of RBC and TAN concentration of rearing water were increased. HRT for maintaining lowest TAN concentration of rearing water was 9.5 minutes and at that condition TAN concentration of rearing tank was $1.03 g/m^3$

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Isolation and Characterization of Acetic Acid Bacteria for Producing "Makgeolli Seed-Vinegar" (막걸리 종초 제조에 적합한 초산균의 분리 및 발효특성)

  • Lee, Hye-Bin;Oh, Hyeonhwa;Jeong, Do-Youn;Jun, Hyun-Il;Song, Geun-Seoup;Kim, Young-Soo
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.46 no.10
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    • pp.1216-1224
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    • 2017
  • Acetic acid bacteria strains were isolated from commercial natural vinegar. Ten isolated strains were identified using 16S rRNA gene sequencing data after evaluating the ethanol- and sulfur-tolerance. Eight of the strains isolated were identified as Acetobacter pasteurianus. A. pasteurianus JAC002, JAC005, and JAC008 strains, which showed a high ethanol tolerance, were selected for making "Makgeolli seed-vinegar". Rice wine vinegars were manufactured with the selected strains through fermentation, and their physicochemical properties and antimicrobial activities were evaluated. A. pasteurianus JAC002 strain showed the highest oxidation ability to acetic acid from ethanol on the twentieth day of fermentation, resulting in 4.21% total acidity, 3,791.77 mg% acetic acid content, and 2,931.78 mg% ethanol consumption content. Rice wine vinegar manufactured with the A. pasteurianus JAC002 strain showed increased antimicrobial activities against Staphylococcus aureus (KACC1927) and Escherichia coli (KACC10115). As a result, A. pasteurianus JAC002 strain was found to be the most suitable strain for "Makgeolli seed-vinegar".

Maturity and Spawning of Spinyhead Sculpin, Dasycottus setiger (Bean) in the East Sea, Korea (동해안 고무꺽정이, Dasycottus setiger (Bean)의 성숙과 산란)

  • Yang, Jae Hyeong;Lee, Sung Il;Hwang, Seon Jae;Park, Jong Hwa;Kwon, Hyeok Chan;Park, Kie Young;Choi, Soo Ha
    • Korean Journal of Ichthyology
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    • v.19 no.3
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    • pp.179-184
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    • 2007
  • Maturity and spawning of spinyhead sculpin, Dasycottus setiger were investigated using samples collected by the eastern sea danish seine and gillnet in the East Sea of Korea from January to December 2004. We analyzed monthly changes in maturity stages, gonadosomatic index (GSI), egg diameter, fecundity, total length at 50% group maturity, and sex ratio. GSI began to increase in August, reached a maximum between September and November, and decreased in December. The spawning period was August to December, and the main spawning period was September to November. The fecundity ranged from 6,210 eggs at 22.3 cm (TL) to 15,898 eggs at 32.2 cm (TL). The relationship between total length (TL) and fecundity (F) was $F=25.475TL^{1.818}$ ($R^2=0.761$), and F increased with TL. The TL at 50% group maturity was estimated to be 23.4 cm. The sex ratio was 1 : 1.3 (female : male), indicating the male population was seem to be slightly predominant.

Screening and Identification of Alkaline Protease Inhibitor-Producing Marine-derived Actinomyces. (Alkaline protease inhibitor를 생산하는 해양유래 방선균의 탐색 및 동정)

  • Kang, Sung-Il;Kong, Jai-Yul;Choi, Yeung-Joon;Kim, Min-Yong;Son, Hong-Joo
    • Journal of Life Science
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    • v.18 no.4
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    • pp.482-487
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    • 2008
  • In this study, we screened and identified the bacterial strain showing high alkaline pretense inhibitor activity from marine environment. Nine bacterial strains with alkaline pretense inhibitor activity were isolated from marine sediments. Among them, strain C12 had the highest alkaline pretense inhibitor activity and was selected for further taxonomical study. On the basis of morphological and physiological characteristics, strain C12 was identified as the genus Streptomyces. A phylogenetic analysis of the 165 rDNA showed that the isolated strain was actually a member of the genus Streptomyces, because the determined sequence exhibited a higher homology with Streptomyces thermocarboxydus. Morphological characteristics showed cylindrical spore chain and smooth spore surface by scanning electron microscope. Strain C12 was grown on all media except for ISP 9 agar. This strain could be grown in the medium containing up to 9% NaCl.

Trajectory Indexing for Efficient Processing of Range Queries (영역 질의의 효과적인 처리를 위한 궤적 인덱싱)

  • Cha, Chang-Il;Kim, Sang-Wook;Won, Jung-Im
    • The KIPS Transactions:PartD
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    • v.16D no.4
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    • pp.487-496
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    • 2009
  • This paper addresses an indexing scheme capable of efficiently processing range queries in a large-scale trajectory database. After discussing the drawbacks of previous indexing schemes, we propose a new scheme that divides the temporal dimension into multiple time intervals and then, by this interval, builds an index for the line segments. Additionally, a supplementary index is built for the line segments within each time interval. This scheme can make a dramatic improvement in the performance of insert and search operations using a main memory index, particularly for the time interval consisting of the segments taken by those objects which are currently moving or have just completed their movements, as contrast to the previous schemes that store the index totally on the disk. Each time interval index is built as follows: First, the extent of the spatial dimension is divided onto multiple spatial cells to which the line segments are assigned evenly. We use a 2D-tree to maintain information on those cells. Then, for each cell, an additional 3D $R^*$-tree is created on the spatio-temporal space (x, y, t). Such a multi-level indexing strategy can cure the shortcomings of the legacy schemes. Performance results obtained from intensive experiments show that our scheme enhances the performance of retrieve operations by 3$\sim$10 times, with much less storage space.

Distribution of Surface Solar Radiation by Radiative Model in South Korea (복사 모델에 의한 남한의 지표면 태양광 분포)

  • Zo, Il-Sung;Jee, Joon-Bum;Lee, Won-Hak;Lee, Kyu-Tae;Choi, Young-Jean
    • Journal of Climate Change Research
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    • v.1 no.2
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    • pp.147-161
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    • 2010
  • The temporal and spatial distributions of surface solar radiation were calculated by the one layer solar radiative transfer model(GWNU) which was corrected by multi layer Line-by-Line(LBL) model during 2009 in South Korea. The aerosol optical thickness, ozone amount, cloud fraction and total precipitable water were used as the input data for GWNU model run and they were retrieved from Moderate Resolution Imaging Spectrometer(MODIS), Ozone Monitoring Instrument(OMI), MTSAT-1R satellite data and the Regional Data Assimilation Prediction System(RDAPS) model result, respectively. The surface solar radiation was calculated with 4 km spatial resolution in South Korea region using the GWNU model and the results were compared with surface measurement(by pyranometer) data of 22 KMA solar sites. The maximum values(more than $5,400MJ/m^2$) of model calculated annual solar radiation were found in Andong, Daegu and Jinju regions and these results were corresponded with the MTSAT-1R cloud amount data. However, the spatial distribution of surface measurement data was comparatively different from the model calculation because of the insufficient correction and management problems for the sites instruments(pyranometer).

Low-temperature aging and drying treatments of restorative rice to improve its microbial safety and texture (복원용 밥의 미생물 안전성 및 식감 향상을 위한 저온 숙성 및 건조 처리)

  • Cheon, Hee Soon;Cho, Won-Il;Chung, Myong-Soo;Choi, Jun-Bong
    • Korean Journal of Food Science and Technology
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    • v.51 no.1
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    • pp.29-34
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    • 2019
  • In this study, a commercial production stepwise method for restorative rice with high quality and microbial safety was developed. The stepwise treatment method included steaming, refrigerated aging, and low temperature drying. The soaking rice was steamed twice at $90-100^{\circ}C$, and then, the rice was aged at $0-10^{\circ}C$, frozen at $-20^{\circ}C$, and dried at low temperatures with 5 m/s wind speed at $1-20^{\circ}C$ and 85% relative humidity. Applying the three steps improved sensory qualities compared with the conventional hot air drying and made storage at room temperature for 3 months possible. Specifically, the moisture content of the restorative rice was increased to 30%, which was 4.3 times higher than the 7% of the conventional air dried rice, and the rice grain shape was well maintained. The texture and appearance of the three-step rice were significantly improved (p<0.05) in a sensory evaluation.

Recognition of Flat Type Signboard using Deep Learning (딥러닝을 이용한 판류형 간판의 인식)

  • Kwon, Sang Il;Kim, Eui Myoung
    • Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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    • v.37 no.4
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    • pp.219-231
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    • 2019
  • The specifications of signboards are set for each type of signboards, but the shape and size of the signboard actually installed are not uniform. In addition, because the colors of the signboard are not defined, so various colors are applied to the signboard. Methods for recognizing signboards can be thought of as similar methods of recognizing road signs and license plates, but due to the nature of the signboards, there are limitations in that the signboards can not be recognized in a way similar to road signs and license plates. In this study, we proposed a methodology for recognizing plate-type signboards, which are the main targets of illegal and old signboards, and automatically extracting areas of signboards, using the deep learning-based Faster R-CNN algorithm. The process of recognizing flat type signboards through signboard images captured by using smartphone cameras is divided into two sequences. First, the type of signboard was recognized using deep learning to recognize flat type signboards in various types of signboard images, and the result showed an accuracy of about 71%. Next, when the boundary recognition algorithm for the signboards was applied to recognize the boundary area of the flat type signboard, the boundary of flat type signboard was recognized with an accuracy of 85%.