• Korean Journal of Veterinary Service
• /
• v.32 no.3
• /
• pp.281-286
• /
• 2009

Fluoroquinolones in muscle and egg were separated by liquid extraction and determined. The analysis was carried out using following conditions; C18 column ($150{\times}4.6mm$, $5{\mu}m$), mobile phase composed of D.W. (containing 0.4% triethylamine and phosphoric acid) : methanol : acetonitrile (780:100:120, v/v/v), quarternary pump at a flow rate of 0.9ml/min and $20{\mu}l$ of injection volume, fluorescence detector with EX 278nm/Em 456nm. The calibration range of seven fluoroquinolones showed linearity ($r^2{\geq}0.999$) at concentration range of $0.025{\sim}0.8{\mu}g/ml$. The recoveries in fortified muscle and egg represented more than 81.3%. The detection limits for ofloxacin, norfloxacin, ciprofloxacin, enrofloxacin, danofloxacin, saraloxacin and orbifloxacin were 3.1, 2.5, 3.6, 1.7, 0.9, 2.5 and $2.1{\mu}g/kg$, respectively. We also monitored fluoroquinolones residue in the sample (chicken muscle 182, cattle muscle 140, pig muscle 139, egg 212) using EEC-plate (E. coli ATCC 11303) screening and HPLC confirmation methods. The screening test results, fluoroquinolones, antibacterial substances were all negative.

• Journal of Korean Society of Steel Construction
• /
• v.29 no.4
• /
• pp.281-289
• /
• 2017

The current AASHTO LRFD and Eurocode 3 specifications have been found to underestimate the flexural strength of longitudinally reinforced plate girders. This is because the web-flange interaction is not considered appropriately when a web is reinforced. The buckling strength of compression flange increases due to the improved rotational restraint to the compression flange. Also, the compression flange and the longitudinal stiffener could constrain the web rotation, so that a certain area of the web reaches yield strength. In this study, a model for evaluating the flexural strength is proposed for plate girders reinforced with one line of longitudinal stiffeners, considering the increase of the buckling strength of the compression flange and the actual stress distribution of the web. The flexural strengths of the conventional steel(SM490) and the high-strength steel(HSB800) plate girders were evaluated from the nonlinear analysis and the applicability of the proposed model was analyzed.

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.38 no.4
• /
• pp.265-275
• /
• 2005

A prerequisite for deriving the abundance estimates from acoustic surveys for commercially important fish species is the identification of target strength measurements for selected fish species. In relation to these needs, the goal of this study was to construct a data bank for converting the acoustic measurements of target strength to biological estimates of fish length and to simultaneously obtain the target strength-fish length relationship. Laboratory measurements of target strength on 15 commercially important fish species were carried out at five frequencies of 50, 70, 75, 120 and 200 kHz by single and split beam methods under the controlled conditions of the fresh and the sea water tanks with the 389 samples of dead and live fishes. The target strength pattern on individual fish of each species was measured as a function of tilt angle, ranging from $-45^{\circ}$ (head down aspect) to $+45^{\circ}$ (head up aspect) in $0.2^{\circ}$ intervals, and the averaged target strength was estimated by assuming the tilt angle distribution as N $(-5.0^{\circ},\;15.0^{\circ})$. The TS to fish length relationship for each species was independently derived by a least-squares fitting procedure. Also, a linear regression analysis for all species was performed to reduce the data to a set of empirical equations showing the variation of target strength to a fish length, wavelength and fish species. For four of the frequencies (50, 75, 120 and 200 kHz), an empirical model for fish target strength (TS, dB) averaged over the dorsal sapect of 602 fishes of 10 species and which spans the fish length (L, m) to wavelength (\Lambda,\;m)$ ratio between 5 and 73 was derived: $TS=19.44\;Log(L)+0.56\;Log(\Lambda)-30.9,\;(r^2=0.53)$.

• Journal of Korean Clinical Nursing Research
• /
• v.14 no.3
• /
• pp.27-36
• /
• 2008

Purpose: This study was to investigate the effects of foot massage on fatigue and depression among patients undergoing chemotherapy with hematological malignancies. Method: The subjects were 60 patients hospitalized for undergoing chemotherapy from September 2007 to March 2008, and 30 of them were the experimental group and the other 30 were the control group. To the subjects in the experimental group, foot massage was provided for 30 minutes everyday before the chemotherapy at the same time for 4 days, and the effect was measured on Day 4 and 5 after chemotherapy. Fatigue was measured using the Brief Fatigue Inventory (BFI) developed by Mendoza et al. (1999), and depression was measured by psychiatric rating scale SCL-90-R developed by Derogatis (1977) and translated by Kim Gwang il et al. The data were analyzed through $x^2$-test, Fisher's exact test, unpaired t-test and repeated measures ANOVA using SPSS 12.0. Results: Fatigue and depression were significantly lower in the experimental group than in the control group. Even on Day 5, fatigue and depression were significantly lower in the experimental group than in the control after finish foot massage. Conclusion: The findings of the study demonstrated that foot massage would be an effective nursing intervention to reduce fatigue and depression in patients with hematological malignancies.

• Progress in Medical Physics
• /
• v.27 no.2
• /
• pp.72-78
• /
• 2016

A CCD camera and an LED light source were combined to fabricate a compact optical CT scanner for the therapeutic radiation dose evaluation of a polymer gel dosimeter. After the collimated beam emitted by the LED passed through aquarium, gel phantom, and telecentric lens, an image was collected by the CCD camera and reconstructed using MATLAB. By using a stepping motor and LabVIEW, the gel dosimeter was rotated at every $0.72^{\circ}$, and the time for collecting 500 slice images per a revolution was within 20 min. At a spatial frequency of 4.5 lp/mm of the optical CT scanner, the modulation transfer function value was 72%. The linear correlation coefficient of the optical CT scanner for the polymer gel dosimeter was 0.987.

• Ocean and Polar Research
• /
• v.41 no.4
• /
• pp.213-232
• /
• 2019

This study evaluates the accuracy of four satellite-composite (OSTIA, AVHRR, G1SST, FNMONC-S) and three model-reanalysis (HYCOM, JCOPE2, FNMOC-M) daily sea surface temperature (SST) data around the Korean Peninsula (KP) using ocean buoy data from 2011-2016. The results reveal that OSTIA has the lowest root mean square error (RMSE; 0.68℃) and FNMOC-S/M has the highest correction coefficients (r = 0.993) compared with observations, while G1SST, JCOPE2, and AVHRR have relatively larger RMSEs and smaller correlations. The large RMSEs were found in the western coastal regions of the KP where water depth is shallow and tides are strong, such as Chilbaldo and Deokjeokdo, while low RMSEs were found in the East Sea and open oceans where water depth is relatively deep such as Donghae, Ulleungdo, and Marado. We found that the main sources of the large RMSEs, sometimes reaching up to 5℃, in SST data around the KP, can be attributed to rapid SST changes during events of strong tidal mixing, upwelling, and typhoon-induced mixing. The errors in the background SST fields which are used in data assimilations and satellite composites and the missing in-situ observations are also potential sources of large SST errors. These results suggest that both satellite and reanalysis SST data, which are believed to be true observation-based data, sometimes, can have significant inherent errors in specific regions around the KP and thus the use of such SST products should proceed with caution particularly when the aforementioned events occur.

• Parasites, Hosts and Diseases
• /
• v.54 no.6
• /
• pp.751-758
• /
• 2016

This study aimed at constructing a draft genome of the adult female worm Toxocara canis using next-generation sequencing (NGS) and de novo assembly, as well as to find new genes after annotation using functional genomics tools. Using an NGS machine, we produced DNA read data of T. canis. The de novo assembly of the read data was performed using SOAPdenovo. RNA read data were assembled using Trinity. Structural annotation, homology search, functional annotation, classification of protein domains, and KEGG pathway analysis were carried out. Besides them, recently developed tools such as MAKER, PASA, Evidence Modeler, and Blast2GO were used. The scaffold DNA was obtained, the N50 was 108,950 bp, and the overall length was 341,776,187 bp. The N50 of the transcriptome was 940 bp, and its length was 53,046,952 bp. The GC content of the entire genome was 39.3%. The total number of genes was 20,178, and the total number of protein sequences was 22,358. Of the 22,358 protein sequences, 4,992 were newly observed in T. canis. Following proteins previously unknown were found: E3 ubiquitin-protein ligase cbl-b and antigen T-cell receptor, zeta chain for T-cell and B-cell regulation; endoprotease bli-4 for cuticle metabolism; mucin 12Ea and polymorphic mucin variant C6/1/40r2.1 for mucin production; tropomodulin-family protein and ryanodine receptor calcium release channels for muscle movement. We were able to find new hypothetical polypeptides sequences unique to T. canis, and the findings of this study are capable of serving as a basis for extending our biological understanding of T. canis.

• Molecular & Cellular Toxicology
• /
• v.5 no.4
• /
• pp.354-363
• /
• 2009

Cytotoxic Nitric oxide (NO) overproduced by inducible NO Synthase (iNOS or NOS2), which was induced in inflammatory reactions and immune responses directly or indirectly affects the functions as host defense and can cause normal tissue damage. Microarray analysis was performed to identify gene profiles of both NO-dependent and -independent transcripts in RAW 264.7 macrophages that use selective NOS2 inhibitors aminoguanidine ($100\;{\mu}M$) and L-canavanine (1 mM). A total of 3,297 genes were identified that were up- or down-regulated significantly over 2-fold in lipopolysaccharide (LPS)-treated macrophages. NO-dependency was determined in the expressed total gene profiles and also within inflammatory conditions-related functional categories. Out of all the gene profiles, 1711 genes affected NO-dependently and -independently in 567 genes. In the categories of inflammatory conditions, transcripts of 16 genes (Pomp, C8a, Ifih1, Irak1, Txnrd1, Ptafr, Scube1, Cd8a, Gpx4, Ltb, Fasl, Igk-V21-9, Vac14, Mbl1, C1r and Tlr6) and 29 geneas (IL-1beta, Mpa2l, IFN activated genes and Chemokine ligands) affected NO-dependently and -independently, respectively. This NO dependency can be applied to inflammatory reaction-related functional classifications, such as cell migration, chemotaxis, cytokine, Jak/STAT signaling pathway, and MAPK signaling pathway. Our results suggest that LPS-induced gene transcripts in inflammation or infection can be classified into physiological and toxic effects by their dependency on the NOS2-mediated NO release.

• Journal of Fisheries and Marine Sciences Education
• /
• v.6 no.1
• /
• pp.58-76
• /
• 1994

Heat transfer and pressure drop measurements are made on low integral-fin tubes in turbulent water flow condition. The integral-fin tubes investigated in this paper are nominally 19mm in diameter. Eight tubes have been used with trapezoidally shaped integral-fins having fin density from 748 to 1654 fpm and 10, 30 grooves. Plain tube having same diameter as finned tube is also tested for comparison. Experiments are carried out using R-11 as working fluid. The refrigerant condensates at a saturation state of $30^{\circ}C$ on the outside tube surface cooled by coolant. The amount of noncondensable gases present in the test loop is reduced to a negligible value by repeated purging. For a given heat input to the boiler and given cooling water flow rate, all test data are taken on steady state. The heat transfer loop is used for testing single long tubes and cooling water is pumped from a storage tank through filters and flowmeters to the horizontal test section where it is heated by steam condensing on the outside of the tube. The pressure drop across the test section is measured by means of pressure gauge and manometer. Each tube tested is cleaned with sodium dichromate pickling solution and well rinsed with water prior to installation in the test section. The results obtained in this study is as follows : 1. Based on inside diameter and nominal inside area, heat transfer of finned tube is enhanced up to 4 times as that of a plain tube at constant Reynolds number and up to 2 times at constant pumping power. 2. Friction factors are up to 1.6~2.1 times those of plain tube. 3. At a given Reynolds number, Nusselt number decrease with increasing pitch to diameter. 4. The constant pumping power ratio for low integral-fin tubes increase directly with the effective area to the nominal area ratio, and with the effective area diameter ratio.

• Journal of the Korean Society of Fisheries and Ocean Technology
• /
• v.51 no.3
• /
• pp.321-332
• /
• 2015

In an attempt to find the improvement of the fishing efficiency according to the hull form remodeling for the 3 tuna purse seiner, the Catch-Per-Unit-Effort (CPUE) for that undertaken before (2008) and after (2010) was analyzed. In addition, the CPUE of 6 similar ships operated same period and same fishing ground. As result, it came to verify that the three modified ships had a significant value on the CPUE. An another index for the improvement of fishing efficiency is the rate of reduction of fuel oil consumption for the modified ships. Fuel oil consumption per day in service speed as comparing with the original ships were reduced 2.1%, 4.0% and 5.1% on the modified ship A, B, and C respectively. And each ship's service speed was increased 1.0 kt, 0.6 kt, and 0.4 kt according to the modified ship A, B, and C in due order. In the conclusion, the remodeling job with newly equipped bulbous bow, lengthened slipway and enlarged rudder area were improved fairly much on fuel oil efficiency, the ship's speed, and in the end, that led to the improving fishing efficiency. Hence, the remodeling of tuna purse seiner come to improve not only the fishing performance, but contribute to the reduction of operating cost by saving energy for the fisheries industry.