• Title/Summary/Keyword: IFA

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Epidemiology of the transmissible gastroenteritis of pigs in Gyeongbuk province (경북지방 돼지에서 발생한TGE의 역학적 특성)

  • 김영환;조광현;김순태;김성국;박인화;손재권
    • Korean Journal of Veterinary Service
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    • v.24 no.3
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    • pp.255-262
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    • 2001
  • Five pigs industry with outbreaks of transmissible gastroenteritis(TGE) in Gyeongbuk province were investigated during the period from January to December 2000. The typical signs of TGE in piglets had transient vomiting and a watery yellowish diarrhea, rapid loss of weight, dehydration and high mortality in pigs under 2 weeks of age. Clinical signs of TGE in growing and finishing pigs and sows were usually limited to inappetence and diarrhea for one or a few days, with vomiting observed in an occasional animal. The detection of TGE viral antigen in epithelial cells of the small intestine had been used in indirect fluorescent antibody test (IFA) for diagnosing TGE in young pigs. WかR had been successfully used to detect the DNA derived from TGEV in specimen of intestinal swabs. Among 5 pigs industry, four showed typical signs of epizootic TGE and one progressing enzootic TGE. It was 22~53 days that the duration of initial clinical disease in TGE outbreaks of pigs investigated in Gyeongbuk province in 2000. However the duration related directly to herd size. Mortality of piglets under 2 weeks of age for duration was 53.2~88.2%, but that of piglets 2~5 weeks of age was 2.5~6.5%. The piglets of 1 weeks of age died mostly during duration of TGE, but varied considerably with husbandry and other environmental factors.

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An epidemiological study on the malaria for residents in Kangwha-Gun(1999) (경기 강화지역 주민에 대한 말라리아의 역학적 특성에 관한 연구(1999년))

  • 김경호;조남규;고종명;김용희
    • Korean Journal of Veterinary Service
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    • v.23 no.1
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    • pp.9-17
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    • 2000
  • Epidemic malaria is prevalent in the demilitarized zone and the northern area of Kyonggi province in Korea. Malaria has broken out in Gangwha-Gun since 1997 due to geographical condition. The results obtained from an epidemiological surveillance for the malaria that happened in 1999 were as follows ; Anopheles sinensis collected in UV-light traps in the barn in Wolgogri and Dangsanri of Songhaemyon showed the distribution of 27,156 of 49,280 figures, corresponding to 55.1%, between July and August. The numbers of patients with malaria were 48(32.4%) in July, 36(24.3%) in August, and 38(25.7%) in September with a prevalence with the ages of the twenties to the seventies. Of a total 154 patients, the male were 86(58.1%) and the female were 68(41.8%). The annual parasite index(API) were 6.44, 5.89 and 4.45 in Songhaemyon, Yangsamyon and Kyodongmyon, respectively. A high infection rate was found Particularly in the areas within 2km from the Han river. By the immunofluorescence assays(IFA), 26 cases were positive with an antibody titers of $\geq$ 1 : 32, of 220 residents in Songhaemyon and 336 ones with a potential case and contacted with the patients. By the blood test, there was no significance in alkaline phosphatase, alanine aminotransferase, total cholesterol, glucose, hemoglobin, hematocrit, white blood cell, red blood cell, and platelet between residents with an antibody titers of $\geq$ 1 : 32 and < 1 : 32 in Songhaemyon.

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Early recognized antigen (p34) of Toxoplasma gondii after peroral ingestion of tissue cyst forming strain (Me49 strain) in mice

  • Park, Yun-Kyu;Nam, Ho-Woo
    • Parasites, Hosts and Diseases
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    • v.37 no.3
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    • pp.157-162
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    • 1999
  • Serum from mouse orally ingested with tissue cyst forming stain (Me49) of Toxoplasma gondii was assayed by Western blot and immunofluorescene assay (IFA) to establish early responses in antigenicity of the parasite in mouse model of foodborne toxoplasmosis. Sera were collected weekly to blot the RH antigen transferred onto nitrocellulose paper after being separated by 12% SDS-PAGE. With the second week serum, 34 kDa protein (p34) was detected uniquely, and all antigens of T.gondii were detected with the sera from 3 or 4 weeks. p34 was not a member of the major surface membrane proteins and confirmed to be localized in the rhoptry by IFA. It was secreted into parasitophorous vacuolar membrane (PVM) during the entry into host cells. 10.3% of sera detected p34, while all the ELISA positive sera detected the band. It has diagnostic usefulness of presumed T.gondii infection. We suggest the name of the p34 protein as ROP9.

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Pathologic Studies in Piglets Naturally Infected with Porcine Reproductive and Respiratory Syndrome Virus (돼지 생식기 호흡기 증후군 바이러스 자연감염 예의 병리학적 연구)

  • Kim, Jae-Hoon;Hwang, Eui-Kyung;Kim, Yong-Joo;Sohn, Hyun-Joo
    • Korean Journal of Veterinary Pathology
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    • v.1 no.2
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    • pp.125-134
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    • 1997
  • Porcine Reproductive and Respiratory Syndrome Virus infection (PRRSV) was confirmed by serology histopathology immunohistochemistry and bacteriologic examination in young pigs. Four suckling and six weaned piglets submitted from three different farms showed coughing sneezing labored rapid abdominal respiration lethargy and anorexia. Grossly apical and cardiac lung lobes appeared mottled with pale to dark tan discoloration. Submandibular and bronchial lymph nodes were tan and enlarged. All piglets were seropositive for PRRSV antibodies by the indirect immunofluorescent antibody(IFA) test. Microscopically lung lesions were characterized by hyperplasia and hypertrophy of type 2 pneumocytes infiltration of mononuclear cells in alveolar intersitium accumulation of necrotic debris in alveolar spaces accompanied by proliferation of alveolar multinucleated syncytial cells. Using immunohistochemical technique PRRSV antigens were demonstrated in alveolar macrophages and type 2 pneumocytes in histologic lung tissue sections. Also PRRSV antigens were detected in brain lymph nodes spleen and heart. Additionally piglets showed nonsuppurative meningoencephalitis mandibular necrotic lymphadenopathy splenic atrophy and myocardial necrosis.

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Detection of Theileria sergenti schizonts in lymphoid cell of lymph node in Korean cattle (한우의 림프절내 림프구양 세포에서 Theileria sergenti schizonts의 검출)

  • Lee, Seung-ok;Lee, Joo-mook;Kwon, Oh-deog;Park, Jin-ho;Park, Sang-youl
    • Korean Journal of Veterinary Research
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    • v.37 no.1
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    • pp.189-194
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    • 1997
  • The life cycle of Theileria sergenti(T sergenti) in cattle, especially Korean native cattle, was not proved clearly. To find schizont stage in the life cycle of T sergenti in Korean cattle, T sergenti schizonts in the cells of parotid lymph nodes from 10 adult Korean cattle were examined. Lymphoid cells which were separated from these lymph nodes were cytocentrifuged to observe the parasites in the cells. T sergenti schizonts were detected in the cells of lymph nodes of 6 cattle out of them by IFA(Indirect Fluorescent Antibody) test and Giemsa stain. By peroxidase stain, the cells which contain schizonts were proved lymphoid cells. T sergenti schizonts identified by IFA test were able to be restained by Giemsa stain. Also, merozoites were observed in peripheral blood of the same 6 cattle that had schizonts, by giemsa stain, but not observed in the 4 cattle that had not been detected schizonts. As a part of life cycle of T sergenti, schizonts were observed in the lymphoid cells of Korean cattle.

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Vlade Hybrid Antenna for the Mobile Phone Handset Terminal (휴대용 단말기를 위한 블레이드 하이브리드 안테나)

  • Oh, Kyu-Jong;Son, Tae-Ho
    • The Journal of the Institute of Internet, Broadcasting and Communication
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    • v.11 no.4
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    • pp.165-169
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    • 2011
  • In recent years mobile phone handset terminal has tended to have additional various functions and slim and light weight, and its internal space is getting smaller and narrower. According to complex parts, small and narrow inner space is caused not sufficient bandwidth and lower impedance. Moreover, additional polarization loss by the surrounding parts produces is lower effectiveness and increases difficulties for getting the antenna gain. For IFA is low profile structure based on the ILA(Inverted L Antenna) which is transformed from the monopole antenna. While this antenna has lots of advantages in internal antenna of mobile phone, it has some disadvantage of reduced bandwidth and gains compared with monopole antenna. In this paper, propose vlade hybrid antenna use for the coupling structure.

FDTD Analysis of the Mutual Coupling Between Closely Placed IFAs (근접한 IFA 사이의 신호결합에 대한 FDTD 해석)

  • Ji, Ki-Man;Lee, Soo-Jin;Chung, Eui-Seung
    • Aerospace Engineering and Technology
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    • v.9 no.1
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    • pp.106-115
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    • 2010
  • Because of space limitations, interferences between antennas of the KSLV-I communication systems occur and their effects become worse during all sorts of tests such as the flight test using a light plane. In this paper, coupled signal magnitude is calculated using the FDTD method. The theory of the FDTD, absorbing boundary condition, source input technique, and post processing of data are explained. The calculated coupling factor between two IFAs, which have 2 GHz resonance frequency and placed 5 cm apart, is -12.7 dB. Applied coupling calculation method can be effectively used for KSLV-I performance analysis, subsystem design, antenna arrangement, and communication link budget for the next space launch vehicle.

Metal Phone MIMO Antenna Using the Auxiliary Letter Slot (보조 문자슬롯을 이용한 메탈폰 MIMO 안테나)

  • Lee, Won-hee;Son, Taeho;Jo, Youngmin
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.27 no.4
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    • pp.395-398
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    • 2016
  • In this paper, a hybrid MIMO antenna for the mobile communication metal phone with auxiliary letter slot is designed and implemented. Auxiliary letter slot locates on the rear metal cover of the phone, and operates on LTE class 40 band. The slots of two letters are designed and operated with monopole+IFA hybrid antenna. Antenna satisfies under VSWR 3: 1 for LTE class 13 / LTE class 14 / CDMA / GSM / DCS / PCS / W-CDMA / LTE class 40 bands. Average gains and antenna efficiencies measured by the anechoic chamber were -5.57~-1.45 dBi and 27.75~71.6 %, respectively.

Analysis of Coupling Between Digital Noise and Portable Smart Terminal Antenna According to Antenna Types (휴대용 스마트 단말기 안테나 타입에 따른 디지털 노이즈와 안테나의 결합 분석)

  • Kim, Joonchul
    • Journal of IKEEE
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    • v.23 no.3
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    • pp.873-877
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    • 2019
  • In this paper, we analyze the degree of digital noise coupling for Inverted F Antenna (IFA) and Loop Antenna, which are representative types of portable terminal antenna, using characteristic mode. Firstly, the degree of coupling according to the direction of digital signal lines and characteristic mode current of the printed circuit board (PCB) including the antenna is compared and analyzed, and based on this result, the coupling between WiFi antenna and the front camera noise is analyzed. For analysis, the digital signal line and ground line of the FPCB of the camera module are modeled as a loop feeder that excites the characteristic mode of the PCB ground and the change of noise coupling according to the antenna types are analyzed.

Survey of antibody on Orientia Tsutsugamushi among wild rodents in Gyeongnam area and detection by nested polymerase chain reaction (경남지역 야생들쥐에서 Orientia tsutsugamushi에 대한 항체 조사 및 PCR에 의한 검색)

  • Hah, Dae-Sik;Kim, Young-Hoon;Park, Jung-Ung;Park, Jae-Kap;Kim, Chung-Hui;Ryu, Jae-Doo;Jong, Myung-Ho;Heo, Jung-Ho;Shu, Jong-Lip;Cho, Myung-Heui;Lee, Kuk-Cheon;Kim, Gon-Sup;Kim, Eui-Kyung;Kim, Jong-Shu
    • Korean Journal of Veterinary Research
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    • v.44 no.2
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    • pp.241-249
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    • 2004
  • As a part of epidemiologic investigation of tsutsugamushi disease, the wild rodents which were captured in Gyeongnam area were diagnosed with indirect immunofluorescent antibody assay (IFA) and Polymerase Chain Reaction (PCR) to find if they have an antibody against Orientia tsutsugamushi. The conclusion was drawn as followings. (1) The captured 58 wild rodents showed that the subspecies distribution of Apodemus agrarius was 86.2%, Microtus fortis was 8.6% and Crocidura lasiura was 5.2%. (2) The antibody positive rate of O. tsutsugamushi Gilliam, Karp, Karto and Boryong by IFA method was 32.0% in Apodemus agrarius among 50 wild rodents and 40.0% in Microtus fortis among 5 wild rodents, respectively. It was negative in the case of all the 3 Crocidura lasiura. (3) The antibody titers on Apodemus agrarius, Microtus fortis and Crocidura lasiura against Gilliam, Karp, Karto and Boryong were measured between 1:20 and 1:640. The antibody titer against each antigen was in the order Boryong>Gilliam>Karp. (4) O. tsutsugamushi was detected from the blood, spleen and kidney from the artificially infected mice by IFA and PCR. IFA showed the positive response between 3 and 18 days after inoculation. On the other hand, positive response was found from all the samples by PCR. (5) From PCR of the genomic DNA extracted from the blood, spleen and kidney samples of the captured wild rodents, Boryong-specific amplification product with size of 210 bp, which is particular in Boryong, was detected from spleen and kidney samples, but not detected in the blood. (6) Boryong-specific amplification product was detected from spleen and kidney samples which were obtained at 3, 6, 12, 18 and 24 days after the infection with Boryong. But, it wasn't detected from the uninfected samples. (7) From PCR of spleen and kidney samples of the captured wild rodents, it was found that positive rate of O. tsutsugamushi in Apodemus agrarius and Microtus fortis were 25.0% (4/16) and 20.0% (1/5), respectively. From the above results, it can be concluded that Apodemus agrarius resided in Gyeongnam area carried O. tsutsugamushi and PCR method might be a simple, precise, rapid and useful diagnostic tool than IFA for the diagnosis of O. tsutsugamushi.