• 한국미생물·생명공학회지
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• 제19권1호
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• pp.45-51
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• 1991

$cI_{857}$ repressor 단백질을 대량으로 얻기위해 tac promoter 하류에 $cI_{857}$ 구조 유전자를 삽입하는 것을 검토하였다. $cI_{857}$ 유전자를 포함하는 DNA 단편을 plasmid PUC12를 이용하여 대량생산후, HphI으로 부분 분해하여 $cI_{857}$ 구조 유전자만을 취하고, tac promoter 하류에 삽입시켰다. 그리고 $\lambda$ phage $cI_{90}$에 의해 $30^{\circ}C$에서는 용원성을, $42^{\circ}C$에서는 용균성을 보이는 균주를 선택함으로 tac promoter 하류에 cI857 구조 유전자가 삽입된 pDR540-$cI_{857}$을 선택할 수가 있었다. 이 plasmid는 $lacI^q$ JM103 뿐만 아니라 각종 E.coli에서 $cI_{857}$-repressor 단백질을 균체 단백질당 약 17까지 생산하였다.

• 한국수산과학회지
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• 제56권1호
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• pp.1-6
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• 2023

Since 2019, Korea Ministry of Oceans and Fisheries has set the annual total allowable catch (TAC) by fish species, and has allocated the annual TAC into each fishery vessel. Also the Korea government plans to adopt the individual transferrable quota system in time. The TAC allocation is similar to the individual fishermen/fishing quota, which the US National Oceanic and Atmospheric Administration has implemented. However, the TAC based management faced fishermen's complaints and a debate is still underway about how to allocate TAC. Because the ideas of the Korea policy are from those in the foreign countries, I intended to provide the Korea fisheries community with an overview about how the TAC has been developed, what problem it caused, and foreign examples of how to allocate it. Furthermore, I pointed out a substantial room for improvement in their current practice of stock assessments, because, otherwise, their current methods for estimation of TAC by species cannot be trusted. Finally I made specific suggestions about what they need to do to reform their current stock assessments.

• 분석과학
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• 제13권3호
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• pp.323-331
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• 2000

표면적과 다공성이 큰 Amberlite XAD-16 수지와 2-(2-thiazolylazo)-p-cresol(TAC) 및 4-(2-thiazolylazo)-orcinol(TAO)를 작용기로 하여 합성한 두가지 킬레이트수지인 XAD-16-TAC 및 XAD-16-TAO를 이용하여 Th(IV), U(VI) 및 Zr(IV)의 흡착특성을 뱃치법으로 조사하였다. 혼합 금속 이온 용액의 pH를 2-6으로 조절하고 24시간 진탕시킨 결과 최적 흡착 pH가 5-6범위이었으며, 가리움제로 NTA, CDTA 및 $NH_4F$를 택하여 혼합 금속 이온의 가리움효과를 조사한 결과 XAD-16-TAC 킬레이트 수지에서 혼합 금속 중 Zr(IV) 이온을, 그리고 XAD-16-TAO 수지로 Th(IV)이온을 분리하는데 $NH_4F$의 효과가 가장 우수한것으로 나타났다. 한편, $HNO_3$를 탈착제로 사용하여 농도를 0.1-2M로 변화시켜 금속이온의 탈착율을 조사한 결과 두가지 킬레이트 수지는 비슷한 경향을 나타내어 2 M에서는 Zr(IV)이온을 제외한 금속이온이 100% 탈착되었다. 그리고 Zr(IV), Th(IV) 및 U(VI)의 혼합금속용액에서 XAD-16-TAC 수지는 Zr(IV)을, XAD-16-TAO 수지는 Th(IV)을 선택적으로 분리할 수 있었다.

• Journal of Microbiology and Biotechnology
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• 제27권2호
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• pp.388-394
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• 2017

Although several analytical methods for measuring total antioxidant capacity (TAC) have been applied to biological samples, there were often dissimilar results due to the different principles of methods applied. Thus, this study aimed to validate four conventional analytical methods for measuring plasma TAC, including the ABTS assay, DPPH assay, FRAP assay, and ORAC assay, by comparing with urinary 8-isoprostane concentration. In addition, TAC results were compared with antioxidant enzyme activities including superoxide dismutase (SOD) and glutathione peroxidase in erythrocyte, and catalase in plasma. Plasma TAC measure by ABTS assay was strongly correlated with the result by FRAP assay. Plasma TAC by FRAP and ORAC assays were negatively correlated with erythrocyte SOD activity. The agreement among the four TAC assay methods and 8-isoprostane was determined using 95% prediction limits of linear regression, expressed as the mean of 8-isoprostane ${\pm}95%$ prediction limits. The ABTS method better agreed with 8-isoprostane than the other methods, demonstrating narrow prediction of limits. Furthermore, only plasma TAC determined by the ABTS assay was inversely correlated with urinary 8-isoprostane (r = -0.35, p < 0.05). In summary, the ABTS assay would be an appropriate method to measure overall plasma antioxidant capacity and predict the body's antioxidant status.

• Journal of Microbiology and Biotechnology
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• 제18권2호
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• pp.334-337
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• 2008

A tightly regulated gene expression system composed of a single-copy target gene under the control of a lac promoter derivative and lacI gene in a multicopy plasmid is proposed, and its ability to control the flux of a metabolic pathway is demonstrated. A model system to control the flux of acetyl-CoA to acetyl phosphate was constructed by integrating pta, a gene encoding phosphotransacetylase, under a tac promoter into the chromosome of E. coli with a pta-negative background and transforming a multicopy plasmid containing the $lacI^Q$ gene into the strain. The production rate of acetate was shown to be tightly controlled when varying the concentration of the inducer (IPTG) in he model system.

• The Korean Journal of Physiology and Pharmacology
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• 제14권4호
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• pp.235-240
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• 2010

Toll-like receptors (TLRs) functionally expressed in salivary epithelial cells, but their roles remain elusive. Among TLRs family, TLR3 is activated by dsRNA, a byproduct of viral infection. The aim of this study was to investigate the role of TLR3 in the inflammatory immune responses using HSG cells. Reverse transcriptase-polymerase chain reaction (RT-PCR), real-time PCR and ELISA were performed to identify expression of TLRs and TLR3-mediated chemokine inductions. The chemotaxis assay of activated T lymphocytes was also performed. Treatment of HSG cells with polyinosinic: polycytidylic acid (poly(I:C)) significantly increased interferon-$\gamma$-inducible protein 10 (IP-10), interferoninducible T-cell $\alpha$ chemoattractant (I-TAC), and regulated on activation, normal T-cells expressed and secreted (RANTES) gene expressions in a concentration-dependent manner. Anti-TLR3 antibody blocked the increases of IP-10 and I-TAC genes. Poly(I:C)-induced increases of IP-10 and I-TAC were also confirmed at protein levels from cell lysates, but their release into extracellular medium was detected only in IP-10. We found that the culture media from HSG cells stimulated with poly(I:C) significantly increases T lymphocyte migration. Our results suggest that TLR3 plays an important role in chemokine induction, particularly IP-10, in salivary epithelial cells.

• Archives of Pharmacal Research
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• 제17권3호
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• pp.194-198
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• 1994

The antitumor effects of the 2E4 and anti--Tac, monoclonal antibodies directed to Il-2 receptor (IL-2R) conjugated with .alpha.-particle emitting radionuclide bismuth-212., were compared. The $^{212}Bi-2E4$ demonstratedspecific cytotocicity to EL4J3, 4, a I$L-2R^+$ cell line, than to EL4J, a $IL-2R^-$ cell line in thymidine incorporation assy. TEX>$^{212}Bi-2E4$ exerted the maximal antitumor effect in that % T/C in C57BL/6 mice implanted with EL4J3.4 ascitic tumor was 331% at the concentration of $50{\;}{\mu}Ci$, while that of $^{212}Bi-anti-Tac$ was 258% at $100{\;}{\mu}Ci$.

• Nutritional Sciences
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• 제6권1호
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• pp.12-19
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• 2003

The measurement of the total antioxidant capacity (TAC) of human plasma has been widely applied in nutritional science, for example to evaluate the antioxidant contribution of dietary components and to study, although indirectly, the bioavailability of dietary antioxidants. Several methods have been proposed for the measurement of TAC, most of them based on the ability of plasma to withstand the oxidative damage induced by aqueous radicals. Although plasma contains both hydrophilic and lipophilic antioxidants that interact through extensive cross-talk in most of the methods employed for the TAC measurement, the hydrophilic antioxidants such as ascorbic acid, uric acid, and protein thiols mainly contribute to the total antioxidant plasma capacity (almost 70%) while lipophilic antioxidants embedded in the lipoproteins (carotenoids, a-tocopherol, ubiquino1-10) participate only in a negligible amount (less than 5%). The present paper reviews the analytical methods used to assess the TAC and in particular focuses on new approaches that are capable of distinguishing the antioxidant capacity of both the aqueous and lipid compartments of plasma. The general principle of the method as well as some in vitro and ex vivo applications will be discussed within the text.

• Food Science and Biotechnology
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• 제17권6호
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• pp.1151-1155
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• 2008

This study aimed to investigate the alterations in plasma antioxidant activity after the consumption of a single oral dose of curcumin, vitamin C, and E administered individually or in combination to (i) assess possible synergies or antagonism between the antioxidants and (ii) determine the optimal composition of the antioxidant mixture such that the duration of action is prolonged to beyond that of individual antioxidants. Each antioxidant was administered to male Sprague-Dawley rats, and blood samples were drawn at different time points up to 180 min to measure the plasma total antioxidant capacity (TAC). Five antioxidant compositions (M1-M5) were evaluated to assess the possible synergies or antagonisms among them and to determine the optimal composition of the antioxidant mixture. Blood samples were collected up to 360 min post-consumption. A single oral dose of individual antioxidants significantly increased the TAC values; however, the time to reach the peak TAC value varied. Among the 5 antioxidant compositions, M2 exhibited the highest and most prolonged antioxidant effect in plasma; this was greater than the proportional sum of the effects of the individual antioxidants in the composition. This result indicates a synergistic interaction among antioxidants in the optimal composition M2.

• Preventive Nutrition and Food Science
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• 제20권3호
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• pp.198-203
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• 2015

The present study was conducted to assess the status of vitamin C among healthy young adults in relation to serum antioxidant parameters [glutathione (GSH), thiols, and total antioxidant capacity, (TAC)], and oxidative stress markers [malondialdehyde (MDA), and nitrites plus nitrates (NN)]. A prospective study included 200 young adults, and their dietary intake was assessed by using food diaries. Fasting plasma vitamin C, serum levels of GSH, thiols, TAC, MDA, and NN were measured using biochemical assays. It was observed that 38% of the enrolled subjects, n=76, had an adequate dietary intake of vitamin C (ADI group). Meanwhile, 62%, n=124, had a low dietary intake of vitamin C (LDI group) as compared to the recommended dietary allowances. The fasting plasma level of vitamin C was significantly higher in the ADI group as compared to the LDI group. Oxidative stress in the sera of the LDI group was evidenced by depletion of GSH, low thiols levels, impairment of TAC, an elevation of MDA, and increased NN. In the ADI group, positive correlations were found between plasma vitamin C and serum antioxidant parameters (GSH, thiols, and TAC). Meanwhile, the plasma vitamin C was negatively correlated with serum MDA and NN levels. This study reveals a significant increase of oxidative stress status and reduced antioxidant capacity in sera from healthy young adults with low intake of the dietary antioxidant, vitamin C.