• Title/Summary/Keyword: I-Domain

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Calculation of Interaction Parameters in Mixed Layer Minerals and their Application (층상형 혼합광물의 상호작용계수의 계산 및 응용)

  • 이성근;김수진
    • Journal of the Mineralogical Society of Korea
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    • v.10 no.2
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    • pp.97-104
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    • 1997
  • Based on the method of determination for relative stability of each phase from the difference among the interaction parameters of the phases consisting the mixed layer, the types of interactions between layers were specified and interaction parameter between layers in ordered domain was analytically derived as a function parameter between layers in ordered domain was analytically derived as a function of not only temperature and mole fraction of layers but also ordering parameter. Interaction parameter between the different layers in ordered phase, L is as follows:{{{{ {L }_{1 } (X,Q,T)= { C} over { Q} -4(1-2Q) { L}^{2 } - { RT} over {2} ln { 1} over {2 } - { 2RT} over { { X}_{ s} } ln { { 4QX}`_{s } ^{2 } } over {(1- { X}_{s }- { QX}_{s })( { X}_{s }- {QX }_{s } ) } }}}}L2 is the interaction parameter between ordered and disordered phase in domain and is the mole fraction of the domain which represent the infinite length of mixed layer mineral and Q and C are the reaction progress parameter and arbitrary constant, respectively. This equation was used for the I/S mixed layer clay minerals to infer the relative stability of R1 type I/S mixed layer in the temperature range from 373K to 450K. The result of calculation suggest that, owing to the decrease in interaction parameter with increasing temperature. The interaction parameter decreases more rapidly with decreasing mole fraction of smectite in domain, which is consistent with the fact that the probability of finding the series smectite layer is lo in the domain with small mole fraction of smectite layers in natural system.

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Intein-mediated expression of Trichoderma reesei Cellobiohydrolase I Cellulose Binding Domain in E. coli (Intein을 이용한 대장균에서의 Trichoderma reesei 유래의 Cellobiohydrolase I 섬유소 결합 도메인의 발현)

  • Choi, Shin-Geon
    • Journal of Industrial Technology
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    • v.36
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    • pp.33-37
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    • 2016
  • Cellulose binding domains (CBDs) of cellulases are thought to assist in the hydrolysis of insoluble crystalline cellulose. To gain sufficient amount of CBDs, the self-cleavable intein tag was used for expression and purification of Trichoderma reesei cellobiohydrolase I CBD in E. coli. Synthetic CBD genes, CBD or linker-CBD were cloned into expression vector pTYB11. Recombinant CBDs were successfully purified by intein mediated purification with an affinity chitin-binding domain. The final yields of recombinant CBD and linker-CBD were 3.2 mg/L and 1.4 mg/L, respectively. The functional bindings of recombinant CBDs were confirmed by Avicel binding experiments. The simple and easy purification method using self-cleavable intein tag can be further used in pretreatment of crystalline cellulose or characterization of engineered CBDs.

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FACTORIZATION AND DIVISIBILITY IN GENERALIZED REES RINGS

  • Kim, Hwan-Koo;Kwon, Tae-In;Park, Young-Soo
    • Bulletin of the Korean Mathematical Society
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    • v.41 no.3
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    • pp.473-482
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    • 2004
  • Let D be an integral domain, I a proper ideal of D, and R =D[It, $t^{-1}$] a generalized Rees ring, where t is an indeterminate. For suitable conditions, we show that R satisfies the ACCP (resp., is a BFD, an FFD, a (pre-) Schreier domain, a G-GCD domain, a PVMD, a v-domain) if and only if D satisfies the ACCP (resp., is a BFD, an FFD, a (pre-) Schreier domain, a G-GCD domain, a PVMD, a v-domain).

A flexible, full-color OTFT-OLED display

  • Yagi, I.;Hirai, N.;Miyamoto, Y.;Noda, M.;Imaoka, A.;Yasuda, R.;Yoneya, N.;Nomoto, K.;Yumoto, A.;Kasahara, J.
    • 한국정보디스플레이학회:학술대회논문집
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    • 2008.10a
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    • pp.1627-1630
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    • 2008
  • We have demonstrated a flexible and full-color OTFT-OLED display. The display has a top-emitting pixel structure with a resolution of 80 ppi, which can be achieved by developed integration architecture of OTFTs. The 0.3-mm-thick flexible display exhibits peak brightness over 100 nit with a contrast ratio greater than 1000:1.

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Co-expression of a novel ankyrin-containing protein, rSIAP, can modulate gating kinetics of large-conductance calcium-activated potassium channel from rat brain.

  • Lim, Hyun-Ho;Park, Chul-Seung
    • Proceedings of the Korean Biophysical Society Conference
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    • 2003.06a
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    • pp.45-45
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    • 2003
  • We isolated a novel ankyrin-repeat containing protein, rSIAP (rSlo Interacting Ankyrin-repeat Protein), as an interacting protein to the cytosolic domain of the alpha-subunit of rat large-conductance Ca$\^$2+/-activated K$\^$+/ channel (rSlo) by yeast two-hybrid screening. Affinity pull-down assay showed the direct and specific interaction between rSIAP and rSlo domain. The channel-binding proteins can be classified into several categories according to their functional effects on the channel proteins, i.e. signaling adaptors, scaffolding net, molecular tuners, molecular chaperones, etc. To obtain initial clues on its functional roles, we investigated the cellular localization of rSIAP using immunofluorescent staining. The results showed the possible co-localization of rSlo and rSIAP protein near the plasma membrane, when co-expressed in CHO cells. We then investigated the functional effects of rSIAP on the rSlo channel using electrophysiological means. The co-expression of rSIAP accelerated the activation of rSlo channel. These effects were initiated at the micromolar [Ca$\^$2+/]$\_$i/ and gradually increased as [Ca$\^$2+/]$\_$i/ raised. Interestingly, rSIAP decreased the inactivation kinetics of rSlo channel at micromolar [Ca$\^$2+/]$\_$i/, while the rate was accelerated at sub-micromolar [Ca$\^$2+/]$\_$i/. These results suggest that rSIAP may modulate the activity of native BK$\_$Ca/ channel by altering its gating kinetics depending on [Ca$\^$2+/]$\_$i/. To localize critical regions involved in protein-protein interaction between rSlo and rSIAP, a series of sub-domain constructs were generated. We are currently investigating sub-domain interaction using both of yeast two-hybrid method and in vitro binding assay.

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Construction of a Fusion-Stoffel Fragment to Improve 3′-5′Exonuclease Activity

  • CHOI, HYEJA;YOUNGSOO KIM
    • Journal of Microbiology and Biotechnology
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    • v.8 no.6
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    • pp.669-675
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    • 1998
  • Taq DNA polymerase exhibits a sizable drawback compared to the other thermophilic DNA polymerases in that it demonstrates lower proof-reading activity due to the deficiency of 3'-5'exonuclease activity. A study was undertaken to improve the 3'-5' exonuclease activity in the PCR of Taq DNA polymerase. The three-dimensional structural alignment of the polymerase and 3'-5' exonuclease domains from the pol I family DNA polymerases explains why Taq DNA polymerase has just a background level of 3'-5'exonuclease activity. A comparison indicated that the two polymerase domains are very similar in primary and tertiary conformations, even though Taq DNA polymerase carries a much shorter 3'-5'exonuclease domain than that of E. coli DNA polymerase I. Those two polymerase domains were interchanged between Taq DNA polymerase and E. coli DNA polymerase I. The 3'-5' exonuclease domain from E. coli DNA polymerase I was separated and pasted into the polymerase domain of Taq DNA polymerase I, which resulted in a functional fusion-Stoffel fragment. The 3'-5'exonuclease activity of the fusion-Stoffel fragment increased up to 48% of the value of the Klenow fragment, while that of Taq DNA polymerase remained at 6.0% of the Klenow fragment.

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KRULL DIMENSION OF HURWITZ POLYNOMIAL RINGS OVER PRÜFER DOMAINS

  • Le, Thi Ngoc Giau;Phan, Thanh Toan
    • Bulletin of the Korean Mathematical Society
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    • v.55 no.2
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    • pp.625-631
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    • 2018
  • Let R be a commutative ring with identity and let R[x] be the collection of polynomials with coefficients in R. There are a lot of multiplications in R[x] such that together with the usual addition, R[x] becomes a ring that contains R as a subring. These multiplications are from a class of functions ${\lambda}$ from ${\mathbb{N}}_0$ to ${\mathbb{N}}$. The trivial case when ${\lambda}(i)=1$ for all i gives the usual polynomial ring. Among nontrivial cases, there is an important one, namely, the case when ${\lambda}(i)=i!$ for all i. For this case, it gives the well-known Hurwitz polynomial ring $R_H[x]$. In this paper, we completely determine the Krull dimension of $R_H[x]$ when R is a $Pr{\ddot{u}}fer$ domain. Let R be a $Pr{\ddot{u}}fer$ domain. We show that dim $R_H[x]={\dim}\;R+1$ if R has characteristic zero and dim $R_H[x]={\dim}\;R$ otherwise.

Individual Roles for Small-sized Web Application Development (소규모의 웹 응용 개발을 위한 역할 분담)

  • 이우진;조용선;정기원
    • The Journal of Society for e-Business Studies
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    • v.6 no.3
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    • pp.209-225
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    • 2001
  • This paper Proposes the individual roles for developing small web application systems based on the Client/Server architecture with the activities and artifacts of each role and cooperation. The roles of Web Server part (i.e. User Interface Designer, Web Designer, HTML Writer), the roles of Application Server part (i.e. Domain Expert, Application Developer, Tester) and the roles of DB Server part (i.e. Database Administrator, Data Designer) are described. Furthermore, the role of the Development Leader that participates in development and manages all works in project and finds the solutions of problems in project, is also discussed. The Domain Expert analyzes the domain of the application in order to send the artifacts to the Application Developer. Then the Application Developer analyzes, designs and implements the application based on the artifacts of the Domain Expert and integrates the implemented program modules. Roles are related each other in this way, and cooperate until the application development is completed. Finally, we analyzed and compared these roles with the roles of RUP(Rational Unified process) and web wave. Suggested roles in this paper turned out to be efficient compared to the roles of the existing large-scale methodology.

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A Case Study on the Opportunity Realization Process of the i-KAIST Venture: Entrepreneurial Intent, Organizational Learning, and Technology/Market Domain Shifts (기업가적 의지, 조직학습, 기술/시장 변화에 의한 대학발 창업 벤처기업의 기회실현 과정: i-KAIST 탐색적 사례연구)

  • Kwon, Sang Jib;Baek, Seoin;Kim, Hee Tae;Chang, Hyun Joon;KIm, Seong Jin
    • Knowledge Management Research
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    • v.14 no.5
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    • pp.55-79
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    • 2013
  • This study primarily focused on the opportunity realization process of a korean venture firm based on university. This research examined the relationship between entrepreneurial intent and organizational learning produced in a sustainable opportunity realization process with technology/market domain shifts. Therefore, this research explores the determinants of sustainable growth of a venture firm at the organizational level and suggests optimal solutions for promoting entrepreneurial intent and opportunity realization for many entrepreneurs. The results showed that CEO's entrepreneurial intent is a key driving factor that can positive impacts on opportunity recognition and organizational learning based on university's expertise. Furthermore, the orientation of a entrepreneur can affect venture's technology/market domain shift through the advanced technological knowledge of university. In conclusion, this research sheds light on the growth of a venture firm based on university suggesting more customized solutions for many entrepreneurs. Implications for the results and the future directions are discussed.

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