• Journal of Microbiology and Biotechnology
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• v.26 no.11
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• pp.1881-1890
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• 2016

Manganese superoxide dismutase (MnSOD) is a vital enzyme that protects cells from free radicals through eliminating superoxide radicals ($O^{2-}$). Hirudin, a kind of small active peptide molecule, is one of the strongest anticoagulants that can effectively cure thrombus diseases. In this study, we fused Hirudin to the C terminus of human MnSOD with the GGGGS linker to generate a novel dual-feature fusion protein, denoted as hMnSOD-Hirudin. The hMnSOD-Hirudin gene fragment was cloned into the pET15b (SmaI, CIAP) vector, forming a recombinant pET15b-hMnSOD-Hirudin plasmid, and then was transferred into Escherichia coli strain Rosetta-gami for expression. SDS-PAGE was used to detect the fusion protein, which was expected to be about 30 kDa upon IPTG induction. Furthermore, the hMnSOD-Hirudin protein was heavily detected as a soluble form in the supernatant. The purification rate observed after Ni NTA affinity chromatography was above 95%. The hMnSOD-Hirudin protein yield reached 67.25 mg per liter of bacterial culture. The identity of the purified protein was confirmed by western blotting. The hMnSOD-Hirudin protein activity assay evinced that the antioxidation activity of the hMnSOD-Hirudin protein obtained was $2,444.0{\pm}96.0U/mg$, and the anticoagulant activity of the hMnSOD-Hirudin protein was $599.0{\pm}35.0ATU/mg$. In addition, in vitro bioactivity assay showed that the hMnSOD-Hirudin protein had no or little cytotoxicity in H9c2, HK-2, and H9 (human $CD_4{^+}$, T cell) cell lines. Transwell migration assay and invasion assay showed that the hMnSOD-Hirudin protein could suppress human lung cancer 95-D cell metastasis and invasion in vitro.

• Journal of the Korean Chemical Society
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• v.19 no.3
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• pp.186-192
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• 1975

In the course of anthracite briquet combustion, air draft is usually controlled to continue burning of definite amount of briquet in the conventional hollow clay cylinder with air inlet hole open for given time, so that a large amount of CO tends to be produced. Therefore, it is necessary to establish an improved combustion process to depress the yielding rate of CO and for this purpose, we performed a basic experiment in which combustion rate of CO was measured in the mixture of $N_2, O_2 $and CO gas with or without the presence charcoal at the various temperature. The observed results showed that the burning temperature of CO is about 680${\sim}700^{\circ}C$, further burning rate of it was increased with increasing the amount of draft. From these facts, longer combustion time and low CO generation are thus contradictory to each other and it has been long desired to make those two compatible somehow. The purpose of the present investigation lies in designing an effective new briquet stove to meet the above requirements. The essential feature of the new briquet stove consisted in the use of two hollow iron cylinders with different inside diameter. A cylindrical air jacket thus formed served as a path through which small amount of secondary air run from the bottom of the stove to the upper vent holes. Heat exchange occurred between the upgoing secondary air and the burning briquet, which lowered the combustion temperature of the briquet. The results observed were selfevident as anticipated. It was confirmed that the combustion time was increased tolerably due to the heat loss from the combustion zone and that CO in the flue gas was reoxidized at the upper portion of the stove by the upgoing hot secondary air. By this reoxidation reaction the concentration of CO in the flue gas was found to be about 1/20 of that in case the conventional clay cylinder was used as briquet jacket.

• Bulletin of the Korean Chemical Society
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• v.24 no.5
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• pp.559-565
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• 2003

An analytical method the determination of benzo(a)pyrene (BaP) and its hydroxylated metabolites, 1-hydroxybenzo(a)pyrene (1-OHBaP), 3-hydroxybenzo(a)pyrene (3-OHBaP), benzo(a)pyrene-4,5-dihydrodiol (4,5-diolBaP) and benzo(a)pyrene-7,8-dihydrodiol (7,8-diolBaP), in rat urine and plasma has been developed by HPLC/FLD and GC/MS. The derivatization with alkyl iodide was employed to improve the resolution and the detection of two mono hydroxylated metabolites, 1-OHBaP and 3-OHBaP, in LC and GC. BaP and its four metabolites in spiked urine were successfully separated by gradient elution on reverse phase ODS $C_{18}$ column (4.6 mm I.D., 100 mm length, particle size 5 ㎛) using a binary mixture of MeOH/H₂O (85/15, v/v) as mobile phase after ethylation at 90 ℃ for 10 min. The extraction recoveries of BaP and its metabolites in spiked samples with liquid-liquid extraction, which was better than solid phase extraction, were in the range of 90.3- 101.6% in n-hexane for urine and 95.7-106.3% in acetone for plasma, respectively. The calibration curves has shown good linearity with the correlation coefficients (R²) varying from 0.992 to 1.000 for urine and from 0.996 to 1.000 for plasma, respectively. The detection limits of all analytes were obtained in the range of 0.01-0.1 ng/mL for urine and 0.1-0.4 ng/mL for plasma, respectively. The metabolites of BaP were excreted as mono hydroxy and dihydrodiol forms after intraperitoneal injection of 20 mg/kg of BaP to rats. The total amounts of BaP and four metabolites excreted in dosed rat urine were 3.79 ng over the 0-96 hr period from adminstration and the excretional recovery was less than 0.065% of the injection amounts of BaP. The proposed method was successfully applied to the determination of BaP and its hydroxylated metabolites in rat urine and plasma for the pharmacokinetic studies.

• Korean Journal of Food Science and Technology
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• v.37 no.5
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• pp.822-826
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• 2005

Effect of Cheonnyuncho extract on the liver injury of rats treated carbon tetrachloride $(CCl_4)$ was studied. Cheonnyuncho extract was administerd at dose of 0.5 and 1 g/kg/day, p.o. for 2 weeks. $CCl_4$ was treated at dose of $0.5\;mL/kg$, i.p. 3 hours later from the last pretreatment of Cheonnyuncho extract. Administration of Cheonnyuncho extract at a dose of 1 g/kg decreased serum AST, ALT and ALP activities by 36, 41, and 22% respectively compared to $CCl_4$ treatment group. Increased lipid peroxidation and decreased SOD and GST activities were also recovered by pretreatment of Chonnyuncho extract in liver of rats. These results suggest that Cheonnyuncho extract has hepatoprotective effect against liver injury.

• Journal of KIISE:Computer Systems and Theory
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• v.30 no.10
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• pp.523-532
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• 2003

Video on Demand(VOD) services cause high resource consumption in a video seuer, because multimedia are characterized by continuous playback, a high bandwidth requirement, and long playback duration. Patching has been proposed to save the network I/O bandwidth of a video server. To achieve true VOD, patching uses multicasting to share video streams, thereby providing immediate VOD services to users without any service latency. A communication channel is used to either multicast the entire video as a regular channel or multicast only the leading portion of a video as a Patching channel. This paper Proposes a buffering patching technique that divides regular channels, as used in patching, into sub-regular channels and regular channels to shorten the holding time of the channels. In the proposed technique, the last portion of video data, corresponding to the size of the buffering window, is not transferred by sub-regular channels, but rather downloaded and buffered in the user buffer from the latest regular channel. When simulations were performed to compare the performance of the proposed technique with that of conventional patching, the results indicated that the proposed technique was superior in terms of the defection rate, average service latency, and fairness, although the amount of video data buffered at each user station was higher than that with patching.

• Journal of KIISE:Computing Practices and Letters
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• v.16 no.1
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• pp.1-10
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• 2010

Recently heterogeneous storage system such as hybrid hard disk drive (H-HDD) combining flash memory and magnetic disk is launched, according as the read performance of NAND flash memory is enhanced as similar to that of hard disk drive (HDD) and the power consumption of NAND flash memory is reduced less than that of HDD. However, the read and write operations of NAND flash memory are slower than those of rotational disk. Besides, serious overheads are incurred on CPU and main memory in the case that intensive write requests to flash memory are repeatedly occurred. In this paper, we propose the Least Frequently Used-Hot scheme that replaces the data blocks whose reference frequency of read operation is low and update frequency of write operation is high, and the data flushing scheme that rearranges the data blocks into the multi-zone of the rotation disk. Experimental results show that the execution time of the proposed method is 38% faster than those of conventional LRU and LFU block replacement schemes in I/O performance aspect and the proposed method increases the life span of Non-Volatile Cache 40% higher than those of conventional LRU, LFU, FIFO block replacement schemes.

• Journal of KIISE:Computer Systems and Theory
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• v.36 no.1
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• pp.26-32
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• 2009

The multi-level storage architecture has been widely adopted in servers and data centers. However, while prefetching has been shown as a crucial technique to exploit sequentiality in accesses common for such systems and hide the increasing relative cost of disk I/O, existing multi-level storage studies have focused mostly on cache replacement strategies. In this paper, we show that prefetching algorithms designed for single-level systems may have their limitations magnified when applied to multi-level systems. Overly conservative prefetching will not be able to effectively use the lower-level cache space, while overly aggressive prefetching will be compounded across levels and generate large amounts of wasted prefetch. We design and implement a hierarchy-aware lower-level prefetching strategy called PMS(Prefetching strategy for Multi-level Storage system) that applicable to any upper level prefetching algorithms. PMS does not require any application hints, a priori knowledge from the application or modification to the va interface. Instead, it monitors the upper-level access patterns as well as the lower-level cache status, and dynamically adjusts the aggressiveness of the lower-level prefetching activities. We evaluated the PMS through extensive simulation studies using a verified multi-level storage simulator, an accurate disk simulator, and access traces with different access patterns. Our results indicate that PMS dynamically controls aggressiveness of lower-level prefetching in reaction to multiple system and workload parameters, improving the overall system performance in all 32 test cases. Working with four well-known existing prefetching algorithms adopted in real systems, PMS obtains an improvement of up to 35% for the average request response time, with an average improvement of 16.56% over all cases.

• Journal of Korean Philosophical Society
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• v.131
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• pp.131-163
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• 2014

This Paper aims to elaborate on the origin of thinking mind. And this is a cooperative project between philosophy and neuroscience and brain science. I have written this paper in admiration for the achievements of twentieth century neuroscience and brain science, and out of desire to assist the subject in future. Much of the history of modern philosophy, from Descartes and Kant forward, consists of failed models of brain. As Edward O. Wilson precisely said, the shortcoming is not the fault of the philosophers, who have doggedly pushed their methods to the limit, but a straightforward consequence of the biological evolution of the brain. Guiding that investigation down pathways that will illuminate brain research is a task of neuroscience and brain science. Investigating logical relations among concepts is a philosophical task. If we are to understand the neural structures and dynamics that make perception, thought, intentional behaviour possible, clarity about these concepts and categories and their relations is essential. Hence our joint venture of philosophy and science. Sure, it is human beings that perceives, not parts of its brain. And it is human beings that who think and reason, not their brain. But the brain and its activities make it possible for human beings-not for it-to perceive and think, to feel emotions, and to form and pursue projects. Thus We try to investigate and reveal the origin of thinking mind as follow: 1) The difference between chimpanzee and human beings 2) brain and mind 3) the origin of thinking 4) the wisdom of nature.

• Parasites, Hosts and Diseases
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• v.59 no.5
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• pp.531-536
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• 2021

The prevalence and intensity of Opisthorchis viverrini metacercariae (OvMc) were investigated in fish from 3 southern administrative regions along the Mekong River in Cambodia, i.e., Phnom Penh, Takeo, and Kandal Provinces from 2017 to 2020. A total of 295 freshwater fish (24 species) were transported to our laboratory with ice and examined using the artificial digestion method. In Phnom Penh, among 4 fish species positive for OvMc, 9 (23.7%) of 38 specimens examined were infected, and their intensity of infection averaged 4.3 metacercariae per infected fish. In Takeo Province, among 10 fish species positive for OvMc, 24 (38.1%) out of 63 fish examined were infected, and their intensity of infection was av. 14.4 metacercariae per infected fish. In particular, all of 3 Osteochilus schlegelii fish examined were infected, and their infection intensity was high, 34.7 metacercariae per fish. In Kandal Province, among 6 fish species positive for OvMc, 46 (90.2%) out of 51 specimens examined were infected, and their infection intensity was 24.0 metacercaraie per infected fish. All fish of Systomus orphoides (n=17), Barbonymus altus (n=14), and Rasbora aurotaenia (n=2) were infected, and their intensity of infection averaged 37.7, 21.6, and 18.5 metacercariae per fish, respectively. Metacercariae of Haplochis yokogawai, Haplorchis taichui, and Centrocestus formosanus were detected in fish from Takeo and Kandal Provinces. From these results, it has been confirmed that a variety of fish species from Phnom Penh, Takeo, and Kandal Provinces are commonly infected with OvMc, and preventive measures to avoid human O. viverrini infection should be performed in Cambodia.

• Journal of Korea Multimedia Society
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• v.9 no.7
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• pp.872-893
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• 2006

In this study, I classify the elements of physiognomic judgment of character with regard to form and meaning from a visual perspective based on physiognomic judgment of character study in 'the cosmic dual forces and the Five Elements theory'. Individual characters for each type are designed using graphic data. Based on that, design system of individual characters for each personality type is investigated using Neural Network system. Faces with O-Haeng (Five Elements) shapes are shown to constitute the system with ${\pm}0.3%$ degree of error tolerance for the non-loaming input data. For the shapes of Chinese characters 'tree, fire, soil, gold and water', their MSE(Mean Square Error) are 0.3, 0.3, 0.2, 0.5, 0.2. It seems to be the best regarding the scoring system which ranges from 0 to 5. Therefore, this system might be regarded to produce the most accurate facial shape of character automatically when we input character's personality we desire to make.