• KOREAN JOURNAL OF CROP SCIENCE
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• v.49 no.6
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• pp.482-486
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• 2004

Benzyladenopurine (BA), a synthetic chemical commonly used for bean sprout culture, should be minimized for wellbeing foods or not be applied. The study was done to check the effect of different BA concentrations treated during 5 hour imbibition on growth and morphological characters of mungbean sprouts. The mungbean seeds of 3 cultivars (Keumseongnogdu, Owoolnogdu, and Zhong Lu 1) were imbibed for 5 hours in the solutions with different BA concentrations (0, 25, 50, 75, and 100 ppm) before 3 hour aeration. On the 6th day after culture, the mungbean sprouts were classified by 4 categories on the base of hypocotyl length; > 7cm, 4 to 7cm, < 4cm, and non-germination, and their morphological characters, fresh and dry weights were measured. Regardless of cultivars the composition rate of hypocotyls of longer than 7cm was decreased with increased BA concentration over 50 ppm while the reverse result was true in the rate of shorter than 4cm. In the rate of 4 to 7cm. cv. Owoolnogdu showed the highest rate in its 50 ppm concentration although cv. Keumseongnogdu and Zhong Lu 1 showed similar result to the above two rates. Formation rate and its number of lateral roots were largely changed around 50 ppm concentration but the roots was not formed in over its 75 ppm concentration. Hypocotyl and root lengths of all the cultivars were shortened with increased BA concentration. In the diameter of middle part of hypocotyl, 3 cultivars showed nearly the same responses as the rate of 4 to 7cm hypocotyls. Hypocotyl and total fresh weights per sprout were heavier in BA treated sprouts than in no treated ones but the weights of the former sprouts were not influenced by its different concentrations.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.49 no.6
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• pp.472-476
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• 2004

Lateral roots formed on mungbean sprouts should lower their quality. The study was carried out to clarify the effects of aeration periods (AP; 1, 2, 3, and 4 hours) and temperature (AT; 20, 30, and $40^{\circ}C$) after 5 hour seed imbibition into 50 ppm benzyladenopurine (BA) solution on growth and morphological characters of mungbean (cv. Keumseongnogdu, Owoolnogdu and Zhong Lu 1) sprouts. On the 6th day, the mungbean sprouts were sorted by 4 categories on the base of hypocotyl lengths; > 7cm, 4 to 7cm, < 4cm, and non-germination to calculate their composition rates, number of lateral roots, lengths of hypocotyl and root, diameters at middle and upper parts of hypocotyl, fraction fresh and dry weights were measured. AP more affected growth of the cultivars than AT showing little effect on them. In the composition rate of the above 4 categories, cv. Zhong Lu 1 had the highest rate in longer than 7cm but nearly the same rate in AP treatments. Rates of longer than 4cm hypocotyls in cv. Keumseongnogdu, Owoolnogdu were increased with longer AP but their rates of shorter than 4cm showed reverse response to the former. Formation rate and number of lateral roots per sprout were decreased with longer AP, showing more severe decrement when delayed 3 to 4 hour AP. Upper part of hypocotyls and roots were more thickened and shortened in longer AP, respectively. Total fresh weights had no significant difference between AP treatments while hypocotyl fresh weights were increased with longer AP. It was concluded that in mungbean sprout culture aeration from BA treatment to the first watering permitted at least 4 hours.

• Journal of Korean Society of Forest Science
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• v.42 no.1
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• pp.67-73
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• 1979

In order to observe the anatomical phenomena of root formation in the hypocotyl cuttings of maples, the hypocotyls of Acer palmatum. Acer micro-sieboldianum, and Acer saccharinum were used as the materials. The rooted portions were sectioned by a microtom and doubly stained by safranin and fast green. The results obtained are summarized as follows; 1. A cross section structure of hypocotyl cuttings of Acer sp. is consist of epidermis, cortex, vascular bundle and pith from the outside. The vascular bundle makes circular shape forming polyarch in A. saccharinum and tetrarch in both A. palmatum and A. micro-sieboldianum. 2. An adventitious root of A. saccharinum originated in phloem parenchyma, while that of A. palmatum and A. micro-sieboldianum originated in interfascicular parenchyma related with phloem cells. 3. The hypocotyl cuttings of Acer sp. is commonly composed of parenchyma tissue having vigorous differentiation capability. Therefore, the originated root grow easily through the cortex and epidermis breaking their tissues.

• Journal of Life Science
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• v.28 no.1
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• pp.9-16
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• 2018

Light is essential to the growth and development of plants, and it is perceived by phytochromes, which are one of the photoreceptors that regulate physiological responses in plants. Ethylene regulates the dormancy, senescence, growth, and development of organs in plants. This research focused on the interaction of phytochromes and ethylene to control hypocotyl growth and gravitropism using phytochrome mutants of Arabidopsis, phyA, phyB, and phyAB, under three light conditions: red (R) light, farred (FR) light, and white light. The mutant phyAB exhibited the most stimulation of gravitropic response of all three phytochrome mutants and wild type (WT) in all three light conditions. Moreover, phyB in the R light condition showed more negative gravitropism than phyA. However, phyB in the FR light condition showed less curvature than phyA. The hypocotyl growth pattern was similar to the gravitropic response in several light conditions. To explain the mechanism of the regulation of gravitropic response and growth, we measured the ethylene production and activities of in vitro ACS and ACO. Ethylene production was reduced in all the mutants grown in white light in comparison to the WT. Ethylene production increased in the phyA grown in R light and phyB grown in FR light in comparison to the other mutants. The ACS activity coincided with the ethylene production in the phyA and the phyB grown in R light and FR light, respectively. These results suggest that the Pfr form of phyB in R light and the Pr form of phyA in FR light increased ethylene production via increasing ACS activity.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.51 no.7
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• pp.608-617
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• 2006

This study was conducted to investigate the effects of sowing date on soybean-sprout growth characters and for improving cultural techniques to produce high quality soybean-sprout. Six sprout-soybean cultivars were planted on three sowing dates in 2000 and 2001. The growth of hypocotyl in soybean sprouts was accelerated at higher temperature and the differences among cultivars became greater at later culture days. The longest of hypocotyl and root lengths of soybean sprouts was obtained from July 5 sowing, followed by May 25 and June 15 sowing. Doremikong and Hannamkong had the thickest and the thinness hypocotyls, respectively. Total length of soybean-sprouts was longer in seeds harvested from later sowing, respectively. Eunhakong produced the longest soybean-sprouts, while Tawonkong produced the shortest. Percentage of rotten seed and imperfectly germinated seed harvested at later sowing was lower. Somyeongkong and Tawonkong showed the highest and the lowest percentages of rotten seeds, respectively, while Pungsannamulkong and Hannamkong showed the lowest and the highest percentages of imperfectly germinated seeds. The soybean-sprouts yields were higher in seeds harvested in 2001 and later sowing. Although Eunhakong and Doremikong showed higher soybean-sprout yield, Pungsannamulkong and Somyeongkong showed stable soybean-sprout yield regardless of the sowing dates.

• Journal of The Korean Society of Grassland and Forage Science
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• v.19 no.3
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• pp.273-280
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• 1999

Hypocotyl explants of Medicago saliva cv. Vernal were cultured on Murashige and Skoog (MS) medium supplemented with various combinations of growth regulators. After six weeks of culture, somatic embryos were formed from calli on MS medium containing $4mg/{\ell}$ 2,4-D and $0.1mg/{\ell}$ kinetin, or $4mg/{\ell}$ 2,4-D and $0.5mg/{\ell}$ kinetin. The mature somatic embryos were developed to plantlets when subcultured on MS basal medium. In order to obtain secondary somatic embryogenic calli, cotyledon of regenerated plantlets were cultured on a callus induction medium. Embryogenic calli were formed on MS medium containing $4mg/{\ell}$ 2,4-D alone. For induction and development of secondary somatic embryogenesis, the embryogenic calli were transferred to MS basal medium containing either 2,4-D or NAA. Multi-secondary somatic embryogenesis was the most effective on MS basal medium with $0.1mg/{\ell}$ 2,4-D. The rate of secondary somatic embryo formation of regenerated plants was 18 times higher than that of seed grown plants. The mature secondary somatic embryo were germinated into plantlets on MS basal medium after six weeks of culture.

• Food Science and Preservation
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• v.10 no.3
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• pp.365-369
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• 2003

The main objective of this study was to investigate the pattern of glucosinolates, in terms of 3-butenyl isothiocyanate, and total glucosinolates, in different parts of young seedlings and seeds in Korean Chinese cabbages. For determination of glucosinolates, 55 days and Winter pride cultivars of Korean Chinese cabbages seeds and different parts of 1, 3, 5, and 7-day-old seedlings were used for analytical sample preparation provided with an anion exchanges column, and measured by GC and UV-visible Spectrophotometer. The concentration of 3-butenyl ITC was higher in the cotyledon and the hypocotyl parts of 55 days cultivar seedling than those of Winter Pride cultivar. In the cotyledon parts of Winter pride cultivar seedling, 3-butenyl ITC amount was increase to 3-day-old seedling and then reduced. The cotyledon of 55 days cultivar contained the highest concentration of total glucosinolates while those were increased in the hypocotyl and decreased in the root. The level of total glucosinolates was increased to 5-day-old seedling and then decreased in the cotyledon and the hypocotyl parts of seedling in Winter pride cultivar. There was no significant difference of total glucosinolates in the root part. In the seeds, 55 days cultivar concentration was higher than Winter pride cultivar both of 3-butenyl ITC and total glucosinolates. In our study, the levels of 3-butenyl ITC and total glucosinolates depended on cultivars, growth stages, and parts in Korean Chinese cabbage.

• Applied Biological Chemistry
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• v.38 no.3
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• pp.212-217
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• 1995

This study was aimed for determining the biochemical mechanism of cold tolerance in crops and for searching the biochemical genetic marker related with cold tolerance by the analysis of isozyme pattern. We investigated various biochemical changes induced by the long-term cold acclimation in cold sensitive rape (B. napus) and in cold tolerant 'Sandongchae'(B. campestris) seedlings. The cold shock after long-term cold acclimation to B. napus and B. campestris greatly increased the activities of peroxidase 157% and 50% in root fraction and, 201% and 205% in hypocotyl, respectively. Simultaneously, the activity of superoxide dismutase was largely increased in hypocotyl fraction, too. Protein contents of hypocotyl fractions in B. napus and B. campestris were also increased by 11.4% and 57.8%, respectively. The band of pl 6.4 among peroxidase isozymes newly biosynthesized during long-term cold acclimation was emerged in the hypocotyl fraction of cold tolerant B. campestris as well as in the root of both species. From above and previous results, we presented a model of interconversions of molecular oxygen species due to the cold injury and biochemically inferred the mechanism of cold tolerance in crops.

• Horticultural Science & Technology
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• v.19 no.1
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• pp.17-21
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• 2001

To develop a selection system for regenerating plants from transformed tissues, effects of four antibiotics (kanamycin, hygromycin, carbenicillin, cefotaxime) and herbicide (phosphinotricin) on shoot regeneration from cotyledon and hypocotyl explants of Chinese cabbage (Brassica campestris L. ssp. pekinensis) were studied. For cotyledon, shoot induction was not significantly affected by kanamycin at $1mg{\cdot}L^{-1}$, but the number of shoots formed was significantly reduced at $2mg{\cdot}L^{-1}$, and no shoots were regenerated from any explants at $6mg{\cdot}L^{-1}$ or higher. Hypocotyl explants showed similar result as cotyledon. Kanamycin at $7mg{\cdot}L^{-1}$ may be adequate for selecting Chinese cabbage transformants. Hygromycin at $4mg{\cdot}L^{-1}$ or higher completely inhibited the growth and shoot regeneration of Chinese cabbage explants. Therefore, resistance gene to hygromycin may also be used as a selective marker for Chinese cabbage transformation. Carbenicillin and cefotaxime, the cephalosporin type of antibiotics, had little effect on shoot regeneration of Chinese cabbage explants. Since carbenicillin and cefotaxime have low toxicity to Chinese cabbage, they are suitable for use in tissue culture to eliminate Agrobacterium in transformation experiments after co-cultivation. Shoot regeneration from cotyledon and hypocotyl explants was significantly reduced in presence of $1mg{\cdot}L^{-1}$ phosphinotricin (PPT) and completely inhibited by $2mg{\cdot}L^{-1}$ or higher. PPT, same as antibiotics, may also be used to select transformed cells. Since Chinese cabbage is known to be recalcitrant to in vitro shoot regeneration compared to other Brassica species, even though lower levels of selectable markers result in more transformants but simultaneously allow more untransformed escapes to develop, lower levels of antibiotics and herbicides could be successfully used as a selectable marker to reduce selection pressure.

• Korean Journal of Breeding Science
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• v.43 no.4
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• pp.311-317
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• 2011

This experiment was conducted to give basic information to sprout-soybean breeding and automated sprout production by investigating the effect of seed size on the sprout growth and texture. Twenty cultivars and lines including large and medium soybean, small interspecific cultivars and extra-small wild soybean lines were used. Seeds were cultured for 4 days using small sprout-culturing kits. Hypocotyl length of large Hwanggeumkong, small Pungsannamulkong and Soyoung were longer and wild soybean lines were shorter than other cultivars. Fresh sprout weight and growth rate per unit dry seed weight of wild soybean lines ware increased dramatically and more than other cultivars between 48 to 72 hours after culture. Not only the increasing rate but sprout yield ratio to used dry seed weight showed the negative relationship pattern with seed size. The hardness of hypocotyl in Jangyeubkong, mastication in Taekwang, and cutting force in Eunhakong were the highest as 3,505 g, 1,650 g, and 133 g respectively, and texture values of these traits in wild soybean YWS516 were the lowest. Cutting force of soybean hypocotyl showed the positive relationship pattern with seed size. Breaking force of hypocotyl in large Jangyeobkong was the highest as 83.5g and wild soybean lines were the lowest showing the same pattern as other textural characters.