• 농업과학연구
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• 제49권3호
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• pp.495-510
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• 2022

In this study, fluorescence hyperspectral imaging (FHSI) was used for the rapid, non-destructive detection of fake, manmade eggs from real eggs. To identify fake eggs, protoporphyrin IX (PpIX)-a natural pigment present in real eggshells-was utilized as the main indicator due to its strong fluorescence emission effect. The fluorescence images of real and fake eggs were acquired using a line-scan-based FHSI system, and their fluorescence features were analyzed based on spectroscopic techniques. To improve the detection performance and accuracy, an optimal waveband combination was investigated with analysis of variance (ANOVA), and its fluorescence ratio images (588/645 nm) were created for visualization of the real eggs between two different egg groups. In addition, real and fake eggs were scanned using a one-waveband (645 nm) handheld fluorescence imager that can perform real-time scanning for on-site applications. Then, the results of the two methods were compared with one another. The outcome clearly shows that the newly developed FHSI system and the fluorescence handheld imager were both able to distinguish real eggs from fake eggs. Consequently, FHSI showed a better performance (clearer images) compared to the fluorescence handheld imager, and the outcome provided valuable information about the feasibility of using FHSI imaging with ANOVA for the discrimination of real and fake eggs.

• 한국안전학회지
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• 제32권6호
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• pp.98-103
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• 2017

In Korea, the guideline for the bridge safety inspection requests to assess surface degradation, including crack, efflorescence, spalling, and so on, for the rating of concrete bridges. Currently, the assessment of efflorescence is performed based on the visual inspection of expertized engineers, which may result in subjective inspection result. In this study, a novel method using a hyperspectral camera is proposed for objective and accurate assessment of concrete efflorescence. The hyperspectral camera acquires the light intensity for a number of continuous spectral bands of light for each pixel in an image, which makes the hyperspectral imaging technique provides more detailed information than a color camera that collects intensity for only three bands corresponding to RGB (red, green, and blue) colors. A stepwise assessment algorithm is proposed based on the spectral features to decompose efflorescence area from the inspected concrete area. The algorithm is tested in the laboratory test using two concrete specimens, one of which is dark colored with efflorescence on a surface while the other is bright concrete without efflorescence. The test shows high accuracy and applicability of the proposed efflorescence assessment using a hyperspectral camera.

• 한국축산식품학회지
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• 제43권6호
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• pp.1150-1169
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• 2023

Edible insects are gaining popularity as a potential future food source because of their high protein content and efficient use of space. Black soldier fly larvae (BSFL) are noteworthy because they can be used as feed for various animals including reptiles, dogs, fish, chickens, and pigs. However, if the edible insect industry is to advance, we should use automation to reduce labor and increase production. Consequently, there is a growing demand for sensing technologies that can automate the evaluation of insect quality. This study used short-wave infrared (SWIR) hyperspectral imaging to predict the proximate composition of dried BSFL, including moisture, crude protein, crude fat, crude fiber, and crude ash content. The larvae were dried at various temperatures and times, and images were captured using an SWIR camera. A partial least-squares regression (PLSR) model was developed to predict the proximate content. The SWIR-based hyperspectral camera accurately predicted the proximate composition of BSFL from the best preprocessing model; moisture, crude protein, crude fat, crude fiber, and crude ash content were predicted with high accuracy, with R² values of 0.89 or more, and root mean square error of prediction values were within 2%. Among preprocessing methods, mean normalization and max normalization methods were effective in proximate prediction models. Therefore, SWIR-based hyperspectral cameras can be used to create automated quality management systems for BSFL.

• Journal of Forest and Environmental Science
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• 제40권1호
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• pp.1-8
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• 2024

Recently, intensive research has been conducted to develop innovative methods for diagnosing plant diseases based on hyperspectral technologies. Hyperspectral analysis is a new subject that combines optical spectroscopy and image analysis methods, which makes it possible to simultaneously evaluate both physiological and morphological parameters. Among the physiological and morphological parameters are classifying healthy and diseased plants, assessing the severity of the disease, differentiating the types of pathogens, and identifying the symptoms of biotic stresses at early stages, including during the incubation period, when the symptoms are not visible to the human eye. Plant diseases cause significant economic losses in agriculture around the world as the symptoms of diseases usually appear when the plants are infected severely. Early detection, quantification, and identification of plant diseases are crucial for the targeted application of plant protection measures in crop production. Hence, this can be done by possible applications of hyperspectral sensors and platforms on different scales for disease diagnosis. Further, the main areas of application of hyperspectral sensors in the diagnosis of plant diseases are considered, such as detection, differentiation, and identification of diseases, estimation of disease severity, and phenotyping of disease resistance of genotypes. This review provides a deeper understanding, of basic principles and implementation of hyperspectral sensors that can measure pathogen-induced changes in plant physiology. Hence, it brings together critically assessed reports and evaluations of researchers who have adopted the use of this application. This review concluded with an overview that hyperspectral sensors, as a non-invasive system of measurement can be adopted in early detection, identification, and possible solutions to farmers as it would empower prior intervention to help moderate against decrease in yield and/or total crop loss.

• Journal of Biosystems Engineering
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• 제43권2호
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• pp.138-147
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• 2018

Purpose: A narrowband hyperspectral imaging sensor of high-dimensional spectral bands is advantageous for identifying the reflectance by selecting the significant spectral bands for predicting crop yield over the broadband multispectral imaging sensor for each wavelength range of the crop canopy. The images acquired by each imaging sensor were used to develop the models for predicting the Chinese cabbage yield. Methods: The models for predicting the Chinese cabbage (Brassica campestris L.) yield, with multispectral images based on unmanned aerial vehicle (UAV), were developed by simple linear regression (SLR) using vegetation indices, and forward stepwise multiple linear regression (MLR) using four spectral bands. The model with hyperspectral images based on the ground were developed using forward stepwise MLR from the significant spectral bands selected by dimension reduction methods based on a partial least squares regression (PLSR) model of high precision and accuracy. Results: The SLR model by the multispectral image cannot predict the yield well because of its low sensitivity in high fresh weight. Despite improved sensitivity in high fresh weight of the MLR model, its precision and accuracy was unsuitable for predicting the yield as its $R^2$ is 0.697, root-mean-square error (RMSE) is 1170 g/plant, relative error (RE) is 67.1%. When selecting the significant spectral bands for predicting the yield using hyperspectral images, the MLR model using four spectral bands show high precision and accuracy, with 0.891 for $R^2$, 616 g/plant for the RMSE, and 35.3% for the RE. Conclusions: Little difference was observed in the precision and accuracy of the PLSR model of 0.896 for $R^2$, 576.7 g/plant for the RMSE, and 33.1% for the RE, compared with the MLR model. If the multispectral imaging sensor composed of the significant spectral bands is produced, the crop yield of a wide area can be predicted using a UAV.

• 한국환경농학회:학술대회논문집
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• 한국환경농학회 2009년도 정기총회 및 국제심포지엄
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• pp.119-126
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• 2009

In recent years, research at the Environmental Microbial and Food Safety Laboratory (EMFSL), Agricultural Research Service (ARS) has focused on the development of novel image-based sensing technologies to address agro-food safety concerns, and transformation of these novel technologies into practical instrumentation for industrial implementations. The line-scan-based hyperspectral imaging techniques have often served as a research tool to develop rapid multispectral methods based on only a few spectral bands for rapid online applications. We developed a newer line-scan hyperspectral imaging platform for high-speed inspection on high-throughput processing lines, capable of simultaneous multiple inspection algorithms for different agro-food safety problems such as poultry carcass inspection for wholesomeness and apple inspection for fecal contamination and defect detection. In addition, portable imaging devices were developed for in situ identification of contamination sites and for use by agrofood producer and processor operations for cleaning and sanitation inspection of food processing surfaces. The aim of this presentation is to illustrate recent advances in the above agro.food safety sensing technologies.

• 대한원격탐사학회지
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• 제34권4호
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• pp.681-692
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• 2018

This study was performed to select the significant wavelengths for predicting the chlorophyll content of grafted cucumber seedlings using hyperspectral images. The visible and near-infrared (VNIR) images and the short-wave infrared images of cucumber cotyledon samples were measured by two hyperspectral cameras. A correlation coefficient spectrum (CCS), a stepwise multiple linear regression (SMLR), and partial least squares (PLS) regression were used to determine significant wavelengths. Some wavelengths at 501, 505, 510, 543, 548, 619, 718, 723, and 727 nm were selected by CCS, SMLR, and PLS as significant wavelengths for estimating chlorophyll content. The results from the calibration models built by SMLR and PLS showed fair relationship between measured and predicted chlorophyll concentration. It was concluded that the hyperspectral imaging technique in the VNIR region is suggested effective for estimating the chlorophyll content of grafted cucumber leaves, non-destructively.

• Journal of Biosystems Engineering
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• 제43권4호
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• pp.369-378
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• 2018

Purpose: The aim of this study was to construct a saponin content-predicting model using shortwave infrared imaging spectroscopy. Methods: The experiment used a shortwave imaging spectrometer and ENVI spectral acquisition software sampling a spectrum of 910 nm-2500 nm. The corresponding preprocessing and mathematical modeling analysis was performed by Unscrambler 9.7 software to establish a ginsenoside nondestructive spectral testing prediction model. Results: The optimal preprocessing method was determined to be a standard normal variable transformation combined with the second-order differential method. The coefficient of determination, $R^2$, of the mathematical model established by the partial least squares method was found to be 0.9999, while the root mean squared error of prediction, RMSEP, was found to be 0.0043, and root mean squared error of calibration, RMSEC, was 0.0041. The residuals of the majority of the samples used for the prediction were between ${\pm}1$. Conclusion: The experiment showed that the predicted model featured a high correlation with real values and a good prediction result, such that this technique can be appropriately applied for the nondestructive testing of ginseng quality.

• 대한원격탐사학회지
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• 제21권4호
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• pp.341-369
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• 2005

이 연구는 새로운 광학원격탐사자료로 대두되고 있는 초분광영상의 기본적 특성과 용어에 관한 정의를 검토하고, 지금까지 초분광영상과 관련된 주요 처리기법 및 활용분야를 광범위하게 검토하여 국내에서 초분광영상 기술의 활용을 위한 기초 자료를 제공하고자 한다. 먼저 문헌자료와 인터넷 검색을 통하여 항공기 및 위성탑재 센서와 지상용 카메라 등 현존하는 초분광센서의 종류 및 특성을 제시하였다 초분광영상과 관련된 연구 현황을 분석하기 위하여 원격탐사와 관련된 주요 국제학술지와 초분광영상 관련 학술발표회에서 발표된 논문들을 선정하여 센서별, 영상처리기법별, 주요 활용분야별로 나누어 정리하였다. 현재 항공기 및 위성 탑재 초분광영상 센서의 종류가 증가하고 있는 추세지만, 지금까지 초분광영상과 관련된 연구의 주된 부분은 미국 항공우주국에서 개발된 AVIRIS영상자료를 토대로 하고 있다. 기존의 다중분광영상에 보다 많은 분광밴드를 가진 초분광영상의 특성을 최대한 이용할 수 있는 영상처리기법이 개발되고 있다. 대기보정, 분광혼합분석, 특징추출 등이 초분광영상처리와 관련된 중요한 분야로 대두되고 있으나, 아직까지 보편적인 초분광영상 처리기술로 자리 잡기까지는 보다 많은 연구가 필요한 실정이다. 초분광영상이 가지고 있는 분광특성 정보를 최대한 이용하기에 적합한 암석 및 광물탐사가 초기의 주된 활용분야였으나, 식물의 물리화학적 정보 추출, 수질, 군용목표물 탐지 등 초분광영상의 활용은 기존의 다중분광영상의 한계를 극복하는 측면에서 확대될 전망이다.

• 대한전자공학회:학술대회논문집
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• 대한전자공학회 2007년도 하계종합학술대회 논문집
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• pp.299-300
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• 2007

Along with the development of imaging sensors, hyperspectral imaging technology is growing rapidly and contributing to many fields of science nowadays. However, the bulky size and complex structure make it difficult to be processed. Focused on in this paper is the clustering utility, implemented in HYVEW, a program involving tools and functions to manipulate with hyperspectral images. The clustering process aims to partition the surface of the imaged area into subregions by grouping the spectra subject to the similarity of spectra.