• The Korean Journal of Zoology
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• v.31 no.2
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• pp.104-110
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• 1988

The comparative karyological analysis of the Korean treefrog, Hyla japonica and Hyla suweonensis performed by conventional giemsa-staining and NOR-staining method. The karyotypes of both species were the same (2n=24). However, according to the Karyological analysis, the 6th chromosomes of the two species distinctly different. Eventhough the 6th chromosomes of the two species shown the same % length, construction of the 6th chromosome of H.japonica was subtelocentric chromosome while H. suweonensis was submetacentric chromosome. This phenomena could explain that the morphological differences in the 6th chromosomes might be caused by pericentric inversion. The two species have 1 pair of NOR site in the 6th chromosome.

• The Korean Journal of Zoology
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• v.33 no.1
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• pp.1-5
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• 1990

The comparative karyological analysis of the Korean treefrogs, Hyla japonica and Hyla suweonensis, were performed by C-banding method. Heteromorphic sex chromosomes, female heterogamety, has been identified in the 3rd chromosomes of H. suweonensis H. suweonensis seem to have sex chromosomes which are Zz/ZW type. The Z chromosomes contain large amount of constitutive heterochromatin, but little heterochromatin is located in the W chromosomes. This is in contrast to all previously known amphibian and most other vertebrate's W or Y chromosomes, except Gastrotheca oui'ern and G. walken (Schmid et al., 1988).

• Journal of the Korean Society of Environmental Restoration Technology
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• v.18 no.1
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• pp.71-82
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• 2015

Hyla suweonensis is an endemic species and is designated as the only endangered species I among amphibians in 2012 by the Ministry of Environment, however studies about its habitat are lacking. This study was carried out to analyze habitat of H. suweonensis based on the spatial information using Maxent (Maximum entropy model as a species distribution model. We detected 45 present points until 2013 and 10 environmental variables by literature review for the model. The results showed that $429km^2$ (0.95%) of the study area, which was about 7.75% of the total agricultural area, was high possible habitats of H. suweonensis. The habitat of H. suweonensis was analyzed by over $1km^2$ rice paddy fields that were lower elevations, flat slopes, and not fragmented. The distance from forests and rivers was identified as a factor that affects its habitat possibilities. In order to conserve H. suweonensis, a large area of rice paddy fields should be preserved, and especially the area around forests and rivers would be required more intensive management. In addition, to compensate for degraded habitats of H. suweonensis in urban areas like as Suwon city, considering integrated watershed management strategy could be effective in the perspective of ecological habitat network of H. suweonensis.

• Animal cells and systems
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• v.3 no.3
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• pp.295-301
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• 1999

The nucleotide sequence of a 447 base pair fragment in the mitochondrial cytochrome b gene and the complete sequence of the mitochondrial 12S ribosomal RNA gene, 938 bp, were analyzed to infer inter- and intraspecific genetic relationships of Hyla japonica and H. suweonensis from Korea and H, japonica from Japan. In the mitochondrial cytochrome b gene, genetic differentiation among H. japonica populations were 9.62% and 15.66% between H. japonica and H. suweonensis. Based on the Tamura-Nei distance, the level of sequence divergence ranged from 0.45% to 2.75% within Korean H. japonica, while 8.31%-8.87% between Korean and Japanese H. japonica and 11.51%-12.46% between H. japonica and H. suweonensis. In the neigh-bor-joining tree, Korean populations of H. japonica were clustered first at 2.22% and followed by Japanese H. japonica and H. suweonensis at 8.51% and 12.29%, respectively. In mitochondrial 12S rRNA gene, genetic differentiation between H. japonica and H. suweonensis nras 7.17% (68 bp) including 7 gaps. Based on Tamura-Nei distance, the level of sequence divergence ranged 3.53% between Korean and Japanese H. japonica and from 4.93% to 5.41% between H. japonica and H. suweonensis. Phenogram pattern of the 12S rRNA gene sequence corresponded with that of the mitochondrial cytochrome b gene.

• The Korean Journal of Zoology
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• v.35 no.2
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• pp.219-225
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• 1992

The genetic variation in mitochondrial DNA (mtDNA) was analysed within and between two species of tree frogs. Hyla japonica and H. suweonensis from South Korea. Purified mtDNAs were digested with each of 11 restriction enLvmes which cleave at six base recognition sequences. The genome size of H. iaponica revealed ho types (20.0 $\pm$ 0.3 and 19.6 $\pm$ 0.3 kb) and this difference is explained by either addition or deletion of about 0.4 kb fragment. On the other hand, the genome sire of H. suueonensis was about 19.0 $\pm$ 0.4 kb only. For the analysis, level of fragment homology (F) and nucleotide sequence divergence (p) were estimated from comparisons of digestion profiles. Among four populations of H. iaponica, substantial mean sequence divergence was 0.017 (range 0.001-0.026); between identical types, 0.001 IslilaRl type) and 0.004 (Large type) respectively; between different ones, 0.024 (range 0.023-0.026). The level of sequence divergence between he species was 0.142 (range 0.131-0.146). This result suggested that he species ㅂwere distinctly differentiated species. The divergence time between ko species was estimated 7.1 million years.

• The Korean Journal of Zoology
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• v.31 no.1
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• pp.11-20
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• 1988

A comparison between two species of Hyla japonica and H. suweonesis in Korea was carried out to clarify their morphological, genetic and ecological differences, to estimate presumed divergent time between them, and to elucidate their probable process and mechanism of speciation. The results are as follows; 1.The breeding season of H. japonica was earlier than that of H. suweonensis about one and half months. 2.H.japonica was distributed all over the country, while H. suweonensis was restricted in Kyonggi plains. 3.On the extemal morphometric characters, differences were hardiy seen between two species. But on the discriminant function analysis, there was apparent differences between them. 4.Genic variation based on 24 loci showed that H. japonica populations were about two fold more variable than that of H. suweonensis populations. 5.Genetic relatedness between two species was rather remote (S =0.520, D=0.634) 6.Their presumed divergent time was estimated to be about three and a quarter mfllon years. It is presumed that H. suweonensis was originated from H. japonica during the period of late pleocene and early pleistocene. 7.Premating isolating mechanisrns (breeding season, mating call, and microhabitat segregation) may be operating to prevent gene ifow between them.

• Animal Systematics, Evolution and Diversity
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• v.31 no.3
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• pp.176-181
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• 2015

Biodiversity is plummeting worldwide, and the major causes of such decline include habitat degradation and climate change. While cities do contribute to the negative impact to the environment, they can also serve as strategic centres for conservation programs. Sites qualifying as biogeographic islands within metropolitan Seoul were studied for the occurrence of two hylid species: the endangered Hyla suweonensis and the abundant H. japonica. This study demonstrates that neither habitat diversity nor surface area, but solely the occurrence of aggregated rice paddies is a requisite for H. suweonensis, hypothetically due to its strict breeding requirements. On the contrary, H. japonica occurrence was not affected by any of these factors, and all types of habitats studied were adequate for this species. The presence of an endangered species within the boundaries of one of the most populated metropolises suggests a strong natural resilience, which should be enhanced with appropriate actions. We emphasize that the management plans therein can, and should, be used as the first step in the conservation of H. suweonensis in metropolitan Seoul.

• Korean Journal of Environment and Ecology
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• v.31 no.4
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• pp.381-396
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• 2017

Understanding species distribution plays an important role in conservation as well as evolutionary biology. In this study, we applied a species distribution model to predict hotspot areas and habitat characteristics for endangered herpetofauna species in South Korea: the Korean Crevice Salamander (Karsenia koreana), Suweon-tree frog (Hyla suweonensis), Gold-spotted pond frog (Pelophylax chosenicus), Narrow-mouthed toad (Kaloula borealis), Korean ratsnake (Elaphe schrenckii), Mongolian racerunner (Eremias argus), Reeve's turtle (Mauremys reevesii) and Soft-shelled turtle (Pelodiscus sinensis). The Kori salamander (Hynobius yangi) and Black-headed snake (Sibynophis chinensis) were excluded from the analysis due to insufficient sample size. The results showed that the altitude was the most important environmental variable for their distribution, and the altitude at which these species were distributed correlated with the climate of that region. The predicted distribution area derived from the species distribution modelling adequately reflected the observation site used in this study as well as those reported in preceding studies. The average AUC value of the eigh species was relatively high ($0.845{\pm}0.08$), while the average omission rate value was relatively low ($0.087{\pm}0.01$). Therefore, the species overlaying model created for the endangered species is considered successful. When merging the distribution models, it was shown that five species shared their habitats in the coastal areas of Gyeonggi-do and Chungcheongnam-do, which are the western regions of the Korean Peninsula. Therefore, we suggest that protection should be a high priority in these area, and our overall results may serve as essential and fundamental data for the conservation of endangered amphibian and reptiles in Korea.