• 제목/요약/키워드: Hydroxyl Radical

검색결과 901건 처리시간 0.034초

산약, 오미자 및 오디 첨가 샐러드 드레싱의 품질 특성 및 항산화성 (Quality Characteristics of Salad Dressing Prepared with Mulberry, Schisandra chinensis and Discorea Powder)

  • 임수빈;김초롱;전혜련;김형돈;이상원;김미리
    • 동아시아식생활학회지
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    • 제22권5호
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    • pp.613-623
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    • 2012
  • In this study, the quality characteristics of salad dressing prepared with mulberry, Schisandra chinensis, and Discorea powder were evaluated. Salad dressing was prepared with salad base (plain yogurt : mayonnaise : fresh cream = 8:1:1), mulberry, Schisandra chinensis for acidity, and Discorea powder for viscosity (4:4:6:1 or 4:4:6:3). Discorea powder was added at levels of 0, 7, and 18% to the salad dressing. As the concentration of Discorea powder increased, the pH level increased, and acidity decreased. Hunter's color L (lightness) value of salad dressing decreased as the amount of Discorea powder increased. Viscosity increased according to the amount of Discorea powder. Moreover, total phenolic content increased according to the amount of Discorea powder. Further, DPPH antioxidant and hydroxyl radical activity of mulberry salad dressing increased as the amount of Discorea powder increased. Sensory preference test results showed that the salad dressing added with Discorea powder had higher scores for color, flavor, taste, and texture than the control. Regarding overall preference, salad dressing containing 7% Discorea powder had the highest scores among the treatments. From these results, salad dressing containing mulberry, Schisandra chinensis for acidity, and Discorea powder for viscosity could be a functional salad dressing with high antioxidant activity.

숙지황 농축액의 첨가량을 달리하여 제조한 갈비찜 소스의 품질 특성 및 항산화성 (Quality Characteristics and Antioxidant Activities of Steamed Rib Sauce Added with Rehmannia glutinosa preparata (Sookjihwang) Concentrate)

  • 나용근;송지혜;전혜련;심은경;이근종;김미리
    • 동아시아식생활학회지
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    • 제22권5호
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    • pp.624-633
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    • 2012
  • This study was performed to evaluate the physiochemical and sensory quality characteristics as well as the antioxidant activities of steamed rib sauce prepared with different amounts of Rehmannia glutinosa preparata concentrate(0, 6, 12, 18%). The moisture content and salinity of control were 80% and 0.8%, respectively, but those of sauces added with Sookjihwang concentrate were not significantly different from those of control. The acidity and reducing sugar content increased according to the amount of Sookjihwang concentrate. Hunter's color L (lightness) and b (yellowness) values decreased according to the amount of Sookjihwang concentrate, whereas a (redness) value was not significantly different among the treatments. Viscosity increased according to the amount of Sookjihwang concentrate. Total phenol and flavonoid contents increased according to the amount of Sookjihwang concentrate. Antioxidant activities (DPPH and hydroxyl radical scavenging activities) also increased according to the amount of Sookjihwang concentrate. Results of sensory preference test revealed that the scores for color, glossiness, and overall preference were the highest in sauce added with 12% Sookjihwang concentrate. It can be suggested that sauce added with Sookjihwang concentrate may be a functional sauce with high antioxidant activity.

A comparative study on the degradation of methyl orange, methylene blue and congo red by atmospheric pressure jet

  • Park, Ji Hoon;Yusupov, Maksudbek;Lingamdinne, Lakshmi Prasanna;Koduru, Janardhan Reddy;Bogaerts, Annemie;Choi, Eun Ha;Attri, Pankaj
    • 한국진공학회:학술대회논문집
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    • 한국진공학회 2016년도 제50회 동계 정기학술대회 초록집
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    • pp.190.1-190.1
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    • 2016
  • One of the most serious problems faced by billions of people today is the availability of fresh water. According to statistics, 15% of the world's total output of dye products is discharged into the environment as dye wastewater, which seriously pollutes groundwater resources. For the treatment of chemically and biologically contaminated water the advanced oxidation processes (AOPs) shows the promising action. The main advantage with AOPs is the ability to degrade the organic pollutants to $CO_2$ and $H_2O$. For this degradation process the AOPs generation of powerful and non-selective radicals that may oxidize majority of the organic pollutants present in the water body. To generate the various reactive chemical species such as radicals (${\bullet}OH$, ${\bullet}H$, ${\bullet}O$, ${\bullet}HO_2$) and molecular species ($H_2O_2$, $H_2$, $O_2$) in large amount in water, we have used the atmospheric pressure plasma. Among the reactive and non-reactive species, the hydroxyl radical (${\bullet}OH$) plays important role due to its higher oxidation potential (E0: 2.8 V). Therefore, in this work we have checked the degradation of various dyes such as methyl orange, methylene blue and congo red using different type of atmospheric pressure plasma sources (Indirect jet and direct jet). To check the degradation we have used the UV-visible spectroscopy, HPLC and LC-MS spectroscopy. Further, to estimate role of ${\bullet}OH$ on the degradation of dyes we have studied the molecular dynamic simulation.

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나노셀룰로오스가 시멘트복합체의 역학적 특성 및 자기수축 특성에 미치는 영향 (Effect of Nanocellulose on the Mechanical and Self-shrinkage Properties of Cement Composites)

  • 김선우;윤병태
    • 공업화학
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    • 제27권4호
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    • pp.380-385
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    • 2016
  • 최근 셀룰로오스 나노피브릴과 셀룰로오스나노크리스탈과 같은 나노셀룰로오스는 관심의 초점이 되고 있다. 나노셀룰로오스의 표면에 있는 수산기는 고분자복합체의 보강재로 사용함에 있어서 적합한 기능을 소유하고 있기 때문이다. 본 연구에서 나노셀룰로오스를 시멘트복합체 제조에 있어서 보강재로서 사용하였다. 나노셀룰로오스는 TEMPO 산화에 의한 전처리과정을 거친 후, 균질화 및 초음파처리에 의해서 제조되었고, 투과전자현미경으로 나노셀룰로오스를 분석한 결과 직경이 10에서 15 nm 범위로 나타났다. 0.5% 나노셀룰로오스가 함유된 시멘트복합체의 압축강도를 기존 시멘트복합체와 비교하였으며 특히, 인장강도와 휨강도가 기존 시멘트복합체에 비해서 각각 49.7%와 38.8% 개선되었다. 그리고 나노셀룰로오스가 혼합된 시멘트복합체의 자기수축률은 타설 후 1일 경과 시 18.9%, 28일 경과 시 5.9%의 저감효과가 나타났다.

Composition of Biologically Active Substances and Antioxidant Activity of New Zealand Deer Velvet Antler Extracts

  • Je, Jae-Young;Park, Pyo-Jam;Kim, Eun-Kyung;Kim, Hyun-A;Lim, Dong-Hwan;Jeon, Byong-Tae;Ahn, Chang-Bum
    • 한국축산식품학회지
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    • 제30권1호
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    • pp.20-27
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    • 2010
  • Deer velvet antler was subjected to the extraction process using boiling water at three different temperatures (100, 110 and $120^{\circ}C$) and 70% ethanol solution. Functional components such as uronic acid, sulfated-glycosaminoglycans (sulfated-GAGs) and sialic acid in the extracts were analyzed, and their antioxidant activities were investigated using several in vitro models. Uronic acid and sulfated-GAGs content of each extract significantly decreased with increasing extraction temperature (p<0.05), while the residues obtained from the upper and middle part of the antler had a higher uronic acid content than the residues obtained from the base section. Sialic acid contents were highest in compounds extracted at $110^{\circ}C$, followed by 120 and $100^{\circ}C$. The 70% ethanol extracts also had a high levels of uronic acid content, but not for sulfated-GAGs and sialic acid. All extracts showed good antioxidant ability in a dose-dependant manner, with the $100^{\circ}C$ residue exhibiting the strongest activity compared to the 110 and $120^{\circ}C$ extracts. In relation to the hydroxyl radical scavenging activity and reduction power, the 70% ethanol extract exhibited the strongest activity. Furthermore, the velvet antler extracts inhibited apoptosis in hydrogen peroxide-induced PC-12 cells.

식품소재 라이브러리를 이용한 천식 완화용 물질의 초고속스크리닝 기법 개발 (Development of High Throughput Screening Techniques Using Food-borne Library against Anti-asthma Agents)

  • 허진철;박자영;권택규;정신교;김성욱;이상한
    • 한국식품저장유통학회지
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    • 제12권3호
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    • pp.267-274
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    • 2005
  • 천식과 관련하여 산화스트레스 (Oxidant stress)는 그 발병요인 중의 하나로 알려져 있다. 이에 본 연구는 농산물과 한약재를 이용하여 항산화 물질을 찾고자 하였으며, 시간과 비용의 단축을 위하여 HTS인 throughput screening)을 이용을 하였다. 항산화 실험과 관련하여서 DPPH(1,1-diphenyl-2-picrylhydrazyl), FRAP(ferric ion reducing antioxidant power), HO(hydroxyl radical) 소거, linoleic acid에 대한 항산화 활성 등을 시행하였다. 이후 $H_{2}O_2$에 의한 산화스트레스를 이용한 세포사멸을 유도하여 세포생존을 확인해 보았다. 실험결과 해바라기씨(Helianthus annuus), 신선초(Angelica utilis Makino), 시금치(Rehmannia glutinosa Libo) 등이 활성이 높게 나타났다. 본 연구는 여기에서 나온 hit를 이용하여 동물모델 실험을 진행하고자 한다.

Fenton-oxidation에 의한 MTBE(Methyl Tertiary Butyl Ether)처리시의 영향인자에 관한 연구 (A Study on the Factors of Fenton-oxidation of MTBE in Water and Soil)

  • 전은미;박석환;정문식
    • 한국환경보건학회지
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    • 제24권3호
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    • pp.63-69
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    • 1998
  • The treatment of soils and water contaminated with MTBE using the Fenton oxidation was investigated. The effects of dosage of $H_{2}O_{2}$, and Fe$^{2+}$ concentrations, and solution pH on transformation and mineralization in soil were evaluated. Generation of TBA and acetone following Fenton-oxidation of MTBE in water and generation of acetone following Fenton-oxidation of TBA were observed. Therefore TBA and acetone are degradation intermediates of MTBE. There was a large difference of treatment efficiency in Fenton oxidation of MTBE between soil and water system. This may be caused by the complex nature of soil, soil organic matter which can consumed OH $\cdot$ radicals, and interacting with inorganic-soil constituents. The pH of soil was observed to have a significant effect on the chemical oxidation efficient of MTBE in soil The data demonstrated that optimal pH range were pH 3~4 and around 6. The soil batch studies demonstrated that treatment efficiency of MTBE was enhanced by adding additional ferrous salts but Fenton-oxidation occurred in no additional iron which indicated that iron in soil can catalyze the Fenton-oxidation. The most effective parameter of Fentonoxidation was $H_{2}O_{2}$/Fe$^{2+}$ ratio which theocratical ratio is 0.5. The optimal range of this ratio was found to be 0.6~2.3. In evaluating effect of $H_{2}O_{2}$ dosage on treatment efficiency, the increase of $H_{2}O_{2}$ did not always lead to increase of decompositions of MTBE in soil. Fenton oxidation was effective in destroying MTBE in aqueous extracts of contaminated soil and water. Experimental data provided evidence that the Fenton oxidation can effectively remediate MTBE-contaminated water and soil.

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연잎의 일반성분, 비타민, 무기질 함량 분석 및 항산화 효과 (Analysis of Chemical Composition, Vitamin, Mineral and Antioxidative Effect of the Lotus Leaf)

  • 이경석;권용준;이기영
    • 한국식품영양과학회지
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    • 제37권12호
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    • pp.1622-1626
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    • 2008
  • 우리나라에 널리 분포하고 있는 연은 식용뿐만이 아니라 민간치료제로도 널리 이용되어져 왔다. 특히 연잎은 오랫동안 사찰에서 차와 술 등으로 제조되어 음용돼 왔으나 이에 대한 연구가 미비한 실정이다. 본 연구에서는 연잎의 성분분석 및 항산화 활성 등의 기초자료를 제공하고자 하였다. 건조한 연잎을 분석한 결과 탄수화물 63.8%, 단백질 16.9%, 지질 1.0%, 조회분 9.3%였다. 엽류 중 녹차와 비교했을 경우 단백질함량은 비교적 낮았고 탄수화물, 지방, 회분의 함량은 높았다. 비타민, 무기질 등 미량성분 분석결과를 녹차와 비교한 결과 비타민과 무기질은 큰 차이를 보여주지 않았으나 칼슘의 함량이 2.2%로 녹차에 비해 20배 이상 높았다. 연잎으로부터 효과적으로 기능성 물질을 추출하기 위해 다양한 용매로 고형분 함량, 총 페놀함량, 항산화력을 측정하였다. 비교실험결과 70% ethanol 추출물을 사용할 때 가장 높은 추출수율인 0.11%를 나타냈다. 또한 용매별 추출물의 총 페놀함량과 free radical 소거능을 측정해 비교한 결과 70% ethanol을 용매로 사용하였을 경우 가장 큰 효과를 나타냈다.

The Expression of DNA Polymerase-$\beta$ and DNA Damage in Jurkat Cells Exposed to Hydrogen Peroxide under Hyperbaric Pressure

  • Sul, Dong-Geun;Oh, Sang-Nam;Lee, Eun-Il
    • Molecular & Cellular Toxicology
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    • 제4권1호
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    • pp.66-71
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    • 2008
  • Long term exposure of Jurkat cells to 2 ATA pressure resulted in the inhibition of cell growth. Under a 2 ATA pressure, the morphological changes in the cells were visualized by electron microscopy. The cells exhibited significant inhibitory responses after three passages. However, short-term exposure study was carried out, 2 ATA pressure may have beneficial effects. The Jurkat cells were exposed to $H_2O_2$ (25 and $50{\mu}M$) in order to induce DNA damage, and then incubated under at either normal pressure or 2 ATA for 1 or 2 hours in order to recover the DNA damage. The extent of DNA damage was determined via Comet assay. More recovery from DNA damage was observed at 2 ATA than at normal pressure. The activity of the DNA repair enzymes, DNA polymerase-$\beta$, was also evaluated at both normal pressure and 2 ATA. The activity of DNA polymerase-$\beta$ was observed to have increased significantly at the 2 ATA than at normal pressure. In conclusion, the effects of hyperbaric pressure from 1 ATA to 2 ATA on biochemical systems can be either beneficial or harmful. Long term exposure to hyperbaric pressure clearly inhibited cell proliferation and caused genotoxic effects, but short-term exposure to hyperbaric pressure proved to be beneficial in terms of bolstering the DNA repair system. The results of the present study have clinical therapeutic application, and might prove to be an useful tool in the study of genotoxicity in the future.

담배 연기 중 산소 자유 라디칼 측정에 의한 품질 평가 (Evaluation of Cigarette Quality by Measurement of Oxygen Free Radicals in Smoke)

  • Ji-Chang Park;Kyung-Ran Yoon;Young-Ha Rhee;Cheong Ho Lee
    • 한국연초학회지
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    • 제12권1호
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    • pp.19-27
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    • 1990
  • 지금까지 연기중의 Nicotine, CO등 몇몇 화합물의 함량 측정과 관능 검사등을 통하여 담배의 품질을 평가해 왔다. 그러나 담배 연기중의 수 많은 화학 성분들을 고려할 때 전연기 성분이 생체에 미치는 영향을 측정함으로써 그 품질을 평가할수 있는 새로운 방범이 요구된다. 담배연기에 의한 생체 손상 쿵 가장 일차적이고 영향이 큰 것으로 알려진 활성 산소종(H2O2, O2-, ·OH)은 그 반응성이 크고 life time이 짧기 때문에 측정이 어렵다. 저자들은 이들을 분해하는 효소를 이용하여 연기중에서 생성되는 이들 산소 자유 라디칼을 측정하였으며 그 결과 담배의 종류에 따라 이들의 생성이 현저한 차이를 보여 일반적으로 고급 담배로 알려진 것들은 낮았고 저급 담배일 수록 높았다. 따라서 이들 산소 자유 라디칼의 측정은 흡연과 건강이라는 측면에서 매우 유용한 담배의 품질 평가 방법으로 이용될수 있을 것으로 생각된다.

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