• Journal of Chest Surgery
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• 제22권1호
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• pp.16-24
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• 1989

To investigate the effects of hydrocortisone on new-born rat cardiac endothelial cells in culture, the endothelial cells were isolated by means of enzyme-cocktail method. The cells were cultivated in Lees modified Dulbeco\ulcorner medium and 10[M or 10[M of hydrocortisone was added to the medium. The cells were harvested or coverglass and processed for thiamin pyrophosphatase reaction and Feulgen reaction. The enzymatic activities of Golgi complex, number of cells and number of large nucleated[more than tetraploid] cells were counted and discussed for their significance. The results were summarized as follows; 1. Hydrocortisone seemed to accelerate the rate of recovery of cardiac endothelial cells from isolation damage. 2. Endothelial cells treated with hydrocortisone revealed strong positive reaction to thiamine pyrophosphatase in early culture and 10 M group had stronger reaction than that of 10 AM group 3. Hydrocortisone had inhibiting effects on endothelial proliferation and the higher the concentration of the reagent was the stronger effects. 4. Hydrocortisone inhibited the appearance of large nucleate cells in endothelial cell population. 5. Hydrocortisone seemed to suppress the nuclear DNA synthesis.

• Biomolecules & Therapeutics
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• 제17권2호
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• pp.205-210
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• 2009

The present study investigated the effects of hydrocortisone on the pharmacokinetics of loratadine in rats after intravenous and oral administration. A single dose of loratadine was administered either orally (4 mg/kg) or intravenously (1 mg/kg) with or without oral hydrocortisone (0.3 or 1.0 mg/kg). Compared to the control group (without hydrocortisone), after oral administration of loratadine, the area under the plasma concentration-time curve (AUC) was significantly increased by 30.2-81.7% in the presence of hydrocortisone (p<0.05). The peak plasma concentration ($C_{max}$) was significantly increased by 68.4% in the presence of 1.0 mg/kg hydrocortisone after oral administration of loratadine (p<0.05). Hydrocortisone (1.0 mg/kg) significantly increased the terminal plasma half-life ($t_{1/2}$) of loratadine by 20.8% (p<0.05). Consequently, the relative bioavailability of loratadine was increased by 1.30- to 1.82-fold. In contrast, oral hydrocortisone had no effects on any pharmacokinetic parameters of loratadine given intravenously. This suggests that hydrocortisone may improve the oral bioavailability of loratadine by reducing first-pass metabolism of loratadine, most likely mediated by P-gp and/or CYP3A4 in the intestine and/or liver. In conclusion, hydrocortisone significantly enhanced the bioavailability of orally administered loratadine in rats, which may have been due to inhibition of both CYP 3A4-mediated metabolism and P-gp in the intestine and/or liver by the presence of hydrocortisone.

• Biomolecules & Therapeutics
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• 제16권3호
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• pp.255-260
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• 2008

The purpose of this study was to evaluate the bioequivalence of the test (Daewoo Hydrocortisone 10 mg, Daewoo Pharm. Co., Busan, Korea) and reference (Jenapharm Hydrocortisone 10 mg, JayTech Biogen, Seoul, Korea) hydrocortisone tablets. Twenty-four healthy male Korean volunteers were divided into two groups with a randomized $2{\times}2$ cross-over design. In order to suppress the endogenous cortisol secretion, a single oral dose of Dexamethasone (4 mg) was administered 10 hr prior to hydrocortisone administration. Blood samples were withdrawn for 10 hr at the predetermined intervals after a single oral dose of hydrocortisone (20 mg). The serum concentration of hydrocortisone was analyzed by HPLC/UV using a column switching method after liquid-liquid extraction process. The pharmacokinetic parameters ($AUC_{0{\sim}10hr}$, $C_{max}$, and $T_{max}$) of the test and reference hydrocortisone tablets were determined while the secretion of endogenous cortisol was being suppressed. The pharmacokinetic parameters of the test tablet were not statistically different from those of the reference tablet at ex value was 0.05. The 90% confidence intervals for the average ratio (test/reference) of $AUC_{0{\sim}10hr}$ and $C_{max}$ were within the Korea Food and Drug Administration acceptance range of 0.80-1.25 ($0.89{\sim}0.99$ and $0.86{\sim}0.99$ for $AUC_{0{\sim}10hr}$ and $C_{max}$, respectively). Therefore it was concluded that the test tablet, Daewoo Hydrocortisone tablet was bioequivalent to the reference tablet, Jenapharm Hydrocortisone tablet.

• 대한미생물학회지
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• 제9권1호
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• pp.41-45
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• 1974

On day 2 after treatment of Lewis rat with 25mg of hydrocortisone, the cell number in the thymus was reduced to less than 10% of the matched control. By day 12 after hydrocortisone treatment the thymic cell population was recovered almost its original value and on day 24 after treatment the number of thymocytes was equivalent to that of normal thymocytes. The density distribution profile of hydrocortisone treated rats as compared with normal rats showed a marked decrease in the denser fraction D while the lighter fractions. (A plus B, and C) showed a considerable proportional increase. The proportion of dead cells in thymus suspension from hydrocortisone treated rats was higher than that from their normal counterparts. On separation, the dead cells accumulated selectively in the pellet and A fraction. The response of the thymocytes from hydrocortisone treated rats to PHA was increased compared to that from normal rats. Among the subpopulations, D fraction, which was relatively unresponsive in normal rats, showed a marked increase in PHA response and C fraction showed some increase in the response.

• Tuberculosis and Respiratory Diseases
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• 제41권5호
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• pp.568-573
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• 1994

저자들은 급성 기관지 천식환자에서 치료제로 사용하는 hydrocortisone을 투여 후 유발된 기관지 수축이 발생한 예를 경험 하였기에 문헌고찰과 함께 보고하는 바이다.

• 한국수정란이식학회지
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• 제17권3호
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• pp.171-177
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• 2002

Glucocorticoid는 비유기 동물의 유선세포 pro-lactin receptor(PRL-R) 발현을 증가시키며, 전반적인 유선세포의 유합성 작용을 활성시킴으로 유합성 능력을 증진시킨다. 유선세포 PRL-R 발현량 증가는 유생산량 향상과 밀접한 관계를 갖는다. 본 실험은 비유중기의 재래산양의 유합성 능력을 향상시키고자, 0.05. 0.1과 0.2 g hydrocortisone을 5ml의 생리식염수에 현탁하여, 정맥투여하고 유선세포 PRL-R와 $\alpha$-유단백 질 mRNAs 발현 량을 조사하였다. 대조구로는 5$m\ell$의 생리식염수를 정맥투여 하였다. 24시간 후 유선조직을 채취하여 $\alpha$-유단백질과 PRL-R mRNA 발현량을 competitive PCR(polymerase chain reaction)로 발현량을 조사하였다. Hydrocortisone 처리 24시간 후 재래산양 유선세포의 PRL-R mRNA 발현량은 대조구의 PRL-R mRNA 발현량과 유의한 차이가 없었다. $\alpha$-유단백질 mRNA 발현은 대조구에 비하여 0.05g hydrocortisone투여구는 37%, 0.1g hydrocortisone 투여구는 630%, 0.2g hydrocortisone 투여구는 386% 증가하였다. Hydrocortison 처리에 의한 유선세포 PRL-R mRNA 발현에 변화가 없었으나 $\alpha$-유단백질 mRNA 발현 증가는 세포 내 기능성 단백질 발현과 세포 외부로 분비되는 단백질의 시간에 따른 발현양상의 차이인 것으로 사려된다. 본 연구에서 비유중기 재래산양에 hydrocortisone 투여는 $\alpha$-유단백질 mRNA 발현을 증가시키는 것으로 나타났다.

• International Journal of Industrial Entomology and Biomaterials
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• 제2권2호
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• pp.181-184
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• 2001

The effect of topical application with 10, 20 and 30 $\mu\textrm{g}$/ml cortisone and hydrocortisone to the fifth stadium larvae of the silkworm, B. mori on fat body glycogen, protein, total lipids, phospholipids, neutral lipids and haemolymph trehalose and protein has been studied. The fat body glycogen haemolymph trehalose significantly decreased in all the treated groups except in the 10 and 20 $\mu\textrm{g}$/ml treated groups. The fat body protein increased significantly in all the cortisone and hydrocortisone treated groups except in the group treated with 10 $\mu\textrm{g}$ hydrocortisone. Whereas that of haemolymph protein significantly increased in all the groups treated with cortisone and hydrocortisone. The total lipids, phospho1ipids and neutral lipids of the fat body decreased significantly in all the groups treated with cortisone and hydrocortisone when compared with that of carrier control.

• Biomolecules & Therapeutics
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• 제8권4호
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• pp.285-292
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• 2000

The effects of the members of the same nuclear receptor superfamily (all-trans retinoic acid (RA), thyroid hormone(T3) or hydrocortisone) on proliferation and differentiation in the SK-N-SH neuroblastoma (NB) cell lines were studied. NB cells were treated with RA, T3, or hydrocortisone at concentration of 10$^{-6}$ M or 10$^{-8}$ M for 3 days or 7 days. RA induced concentration- and time-dependent morphologic differentiation(neurite outgrowth and microtubule-associated protein expression) and growth inhibition in NB cells. Treatment of 10$^{-7}$ M T3 for 7 days increased viability and differentiation of NB cells. Treatment of 10$^{-6}$ M hydrocortisone for 7 days increased viability of NB cells. Although these three effectors are members of the same receptor superfamily, the regulation of brain development may be carried out in a different manner.

• 대한약리학회지
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• 제10권2호
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• pp.63-74
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• 1974

This study was carried out to observe the direct effect of hydrocortisone on renal function by infusing it into a renal artery. Hydrocortisone (5mg/kg) or saline (0.5 ml/kg) was infused directly into the left renal artery of the rabbit, the right kidney was left intact to serve as a control for general action of acetazolamide (10 mg/kg) or aminophylline (10 mg/kg), which was administered intravenously 30 minutes after the direct infusion of pretreated drugs (hydrocortisone or saline). The changes of urine volume, pH, urinary excretion rates of $Na^+,\;K^+\;and\;Cl^-$, and the clearances of inulin and PAH were measured at an interval of 10 minutes for half an hour after the direct infusion of hydrocortisone or saline, and for one hour after intravenous administration of acetazolamide or aminophylline. The results of the experiment were as follows: 1. Significant changes in urine volume and urinary electrolytes (excreted rates of $Na^+,\;K^+\;and\;Cl^-$) were observed in the hydrocortisone-infused group 10 minutes after the administration of acetazolamide, compared with the saline-infused group. Especially, the effect was more potent on the infused (left) side than on the contralateral (right) side. 2. Significant changes in urine volume and urinary electrolytes were also observed in all the aminophylline-treated groups, but no remarkable difference was noticed between the hydrocortisone-infused group and the saline-infused group, nor between the left and right sides. 3. No signicant changes in the clearances of inulin and PAH were in the infused (left) side of all the experimental groups, as compared with the contralateral (right) side. From the above results, it is obvious that hydrocortisone infused into a renal artery exerts diuretic action when administered in combination with acetazolamide, and the mechanism of action rests not on its hemodynamic change for renal blood flow, but on the potentiation of carbonic anhydrase inhibiting action. However, the exact mode of action remains yet to be clarified.

• 대한한방내과학회지
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• 제21권3호
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• pp.389-398
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• 2000

The fallowing are the results of the experimental studies of Cordyceps militaris (CM) on the penile nitric oxide synthase (NOS) activity and the level of blood testosterone in hydrocortisone acetate-treated rats. CM was tested for the effects on activity of xanthine oxidase and lipid peroxidation in penis of hydrocortisone acetate-treated rats. In vitro, CM didn't effect the levels of lipid peroxide and the activity of NOS. In the penis of hydrocortisone acetate-treated rats, lipid peroxide, the activities and ratio of type conversion of xanthine oxidase were increased but activity of NOS and content of nitrite were decreased. In vivo, after administration of CM to hydrocortisone acetate-treated rats, levels of lipid peroxide in penis was decreased. Also, the activities and ratio of type conversion of xanthine oxidase were decreased, too. The body weight and concentration of testosterone in the blood were increased. The effects of Cordyceps militalis Broth did better than the effects of Cordyceps militalls Mycelia, These results suggest that CM decrease the activities of free radical generating enzymes such as xanthine oxidase which form lipid peroxide and increase the penile NOS activity and the level of blood testosterone in hydrocortisone acetate-treated rats. Conclusively, CM is capable of improving of sexual ability in hydrocortisone acetate-treated rats.