• Korean Journal of Plant Taxonomy
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• v.34 no.1
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• pp.1-7
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• 2004

An unrecorded species in Korean flora, Hydrangea luteovenosa Koidz. (Hydrangeaceae) was collected from parasitic cone, Seongneol-orom region of Mt. Halla in Prov. Jeju-do. This species is readily distinguishable from H serrata (Thunb.) Ser. and H. petiolaris Siebold & Zucc. in Korea by habit, length and width of leaves, length of petiole, morphology of petals and leaf margins, position of ovary, number of stamens and secondary veins.

• Korean Journal of Plant Taxonomy
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• v.47 no.1
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• pp.1-5
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• 2017

The population status of Hydrangea luteovenosa Koidz. in Korea was investigated, with an emphasis on its genetic diversity. From field surveys, we obtained the only locality record for a wild population in Jeju Island, which contained 285 individuals in total. Genotyping was performed using five microsatellite markers for the all extant plants in Korea. Three Japanese populations were also genotyped for the comparative analyses. The genotyping result showed that the Jeju population consisted of only two multilocus genotypes, including identical heterozygous genotypes at two loci; it had been maintained mostly by vegetative reproduction; and although the Jeju population is geographically far from Japanese populations, all alleles observed in the Korean population were shared with Japanese populations, suggesting the possibility that H. luteovenosa in the Jeju Island had been recently migrated or introduced from Japan. Future ecological and genetic studies associated with negative effects of low genetic variation will be essential for determining the conservation direction of the threatened Korean population of this species.

• Korean Journal of Pharmacognosy
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• v.9 no.3
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• pp.149-156
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• 1978

The result of the study about the cultivation, the constituents, the sweetenity, and the development of preparations of Stevia and Hydrangea are as follows: 1. Hydrangea thrived at $200{\sim}1,400m$ above the sea-level, and the good ones were brought up at $600{\sim}1,200m$. 2. The good ones which belonged to the genera of Hydrangea were produced in the damp, dark and cold place. 3. The growth and cultivated yield of Stevia was not significantly influenced by the acidity of the soil. 4. The best season was May and June for propagation of Stevia by cutting method. 5. The growth rate of Stevia and Hydrangea was really good in the sandy fertile soil, but in the mucotic soil it was not suitable. 6. The extraction and separation of phyllodulcin by solvent had many difficulties, because it was very soluble in water, ethanol, ether, petroleum ether, acetone and benzene, etc. 7. The solubility of stevioside on the solvent was as follows: It was very soluble in water and methanol, slightly soluble in ethanol and acetone, and insoluble in ether, petroleum ether and chloroform. 8. The alkaloid reaction by Mayer reagent in Hydrangea extract was positive. 9. The ashification rate of Stevia was 8.66% to 8.72% and that of Hydrangea 17.02% to 17.04%. 10. The tannin of Stevia leaf was 7.80% to 7.88% and its of Hydrangea decreased 9.46% to 6.08% by fermentation. 11. The percent rates in minimum concentration-occurring sweetness in sugar, glycyrrhiza methanol extract, Hydrangea water extract, Stevia leaf water extract, decoction of Stevia's leaf, decoction of Stevia's stem were as follows: 1.2, 0.1, 0.1, 0.2, 0.4, 0.01, 0.1, 0.6. and sweetenity ratios of those were 1, 12, 12, 6, 3, 64, 12, 2, 12. It was very meaningful to develop preparations of stevia as stevioside, micronized powder, water extract, methanol extract and compound teas and in Hydrangea, water extract, methanol extract, single tea, and compound teas were less meaningful. 13. The genera of Hydrangea which is natural species in Korea was positive in phyllodulcin-identification test, but it was not available to make the sweet tea because of having a little content.

• Korean Journal of Plant Taxonomy
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• v.43 no.1
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• pp.30-33
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• 2013

Hydrangea luteovenosa is a critically endangered plant species of Jeju Island in Korea, though it is widely distributed in western Japan. We isolated and characterized five microsatellite loci in this species. The number of alleles ranged from 3 to 27, observed heterozygosity from 0.27 to 0.86, and expected heterozygosity from 0.34 to 0.91. The markers described here will be useful for investigating the genetic diversity, genetic structure, and gene flow of H. luteovenosa, and the genetic findings would contribute to the establishment of effective conservation measures for this species in Korea.

• Journal of Korean Society of Forest Science
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• v.86 no.3
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• pp.319-323
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• 1997

Perforated ray cells are identified for the first time in the Korean species of Deutzia glabrata, Deutzia sieboldiana, Hydrangea paniculata, and Philadelphus schrenckii that belong to Hydrangeaceae but not observed in Deutzia gracilis. These ray cells have simple to scalarifrom perforations in Deutzia glabrata which have vessel elements with scalariform perforations, and have scalariform to reticulate perforations in Deutzia sieboldiana, Hydrangea paniculata, and Philadelphus schrenckii which have vessel elements with scalariform perforations. Thus, the perforations of ray cells in Korean Hydrangeaceae appeared not to be exactly the same as the types of scalariform perforation plates in the vessel elements of same wood.

• Korean Journal of Plant Resources
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• v.29 no.4
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• pp.407-418
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• 2016

In this study, the phylogenetic relationship of Korean Hydrangea was evaluated by using sequenced three chloroplast regions and ITS region, including the 7 taxa. The result of phylogenetic analysis indicated that Korean Hydrangea, 7 taxa formed the monophyletic group. This analysis also revealed that subsect. Macrophyllae of Korea was separated into two groups; H. serrata f. acuminate and H. macrophylla group. The H. serrata f. acuminta group was included with H. serrata f. buergeri and H. serrata f. fertilis. These three species form a monophyletic clade, with no significant differences between their nucleotide sequences. The H. serrata f. acuminta group showed a monophyletic group with H. serrata f. buergeri and H. serrata f. fertilis and there is significant differences between their nucleotide sequences. H. macrophylla group was an independent clade distinguished by H. serrate f. acuminate group. Subsect. Petalanthe, Heteromallae and Calyptranthae form a monophyletic group. H. petiolaris which is located in Subsect. Calyptranthae was separated into two subgroups; First subgroup: Jeju island (except for Mt. Halla) and Second subgroup: Ulleung island and Japan. Additional studies of two subgroups of H. petiolaris should be conducted a geographical study and add more samples.

• Journal of the Korean Institute of Landscape Architecture
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• v.22 no.4
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• pp.149-160
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• 1995

We investigated the seasonal changes flower color of 163 deciduous woody landscape plants in the Suwon region from January 1, 1992 to March 20, 1993. The results were as follows; 1. By the month of anthesis of woody landscape plants, only one plant of Hamamelis japonica flowered in February, 15 species in March, 48 species in April, 63 species in May, 23 species in June, 12 species in July, and one plant of Hydrangea paniculata was flowered in August. 2. The flowering period was about 220 days from February 24, 1992 that Hamamelis japonica was anthesis to October 5, 1992 when Hydrangea paniculata was deblossomed. 3. By the flowering period of woody landscape plants, 81 species continued for 11 days through 20 days, and Rosa spp., 118 days, Hibiscus syriacus 'Yungkwang', 80 days, Largerstroemia indica, 65 days, and 6 species continued for 41 through 60 days, 10 species were 31 through 40 days, 43 species were 21 through 30 days, and 20 species were for less than 11 days. 4. The woody landscape plants flowering before leaf spreading, Hamamelis japonica, Abeliophyllum distichum, Prunus mume 'Hwahyangmi', Prunus mume 'Baekkaha', Lindera obtusiloba, Cornus officinalis, Prunus armeniaca. The others were plants with leaves spreading white flowering; Forsythia ovata 'Tetra gold', Forsythia ovata, Corylus hetrophylla, Rhododendron mucronulatum, Magnolia denudata, Forsythia koreana 'Seoul Gold', Forsythia koreana, Magnolia stellata, Acer negundo 'Elegans', Magnolia kobus, Forsythia viridissima 'Bronxensis', Prunus yedoensis, Prunus leveilleana var. pendula, Prunus persica for. albiplena, Prunus tomentosa, Prunus persia, Magnolia liliflora, Prunus glandulosa for. sinensis, Cercis chinensis, Poncirus trifoliata. 5. In terms of flower color based on KBS standard color number, 83 species were white, 44 species wer red, 21 species were yellow, 12 species were violet, and 3 species were green. 6. In terms of the flower color by month. Hamamelis japonica was yellow February. Flower colors in March were : yellow-7 species, red-3 species and white-5 species. Flower colors in April were : White-21 species, red-19 species and yellow-6 species. Flower colors in May were : White-36 species, red-16 species. The white flowers in June were 16 species. Flower colors in July were : white-4 species, red-4 species. 7. The white flower color of woody landscape plants of trees was 35 species. The red flower color was 18 species, yellow flower color was 5 species, violet flower color was 2 species, and green flower color was 3 species. Also the white flower color of woody landscape plants of shrubs was 48 species, red flower color was 25 species, yellow flower color was 17 species and violet flower color was 10 species. 8. The new 'Cultivars' of woody landscape plants are needed to introduced the development of planting design. 9. Present data of illustrated books of plants should be checked by new data that was studied in this research.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2019.10a
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• pp.22-22
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• 2019

수국속(Hydrangea L.)은 APG IV분류체계에 따르면 국화군(Asterids), 층층나무목(Cornales), 수국과(Hydrangeaceae)에 속하는 식물로서 동아시아, 북아메리카 동부 지역을 중심으로 분포하며 전 세계에 23여종이 알려져 있다. 한국산 수국속은 Nakai가 H. hortensia var. acuminata의 분포를 기록함으로써 시작되었다. 이후 Nakai는 산수국을 H. acuminata로 재처리, 다시 H. serrata var. acuminata로 변경하였다. 한편 산수국은 분류학적 위치에 대한 이견이 있다. Wilson은 수국과 산수국을 가까운 유연관계를 가지는 독립된 분류군으로 분류하였지만, Makino는 산수국을 수국의 아종인 H. marcrophylla subsp. serrta로 기재하는 등 산수국과 수국의 동정과 계급설정에 혼란이 있는 가운데, 최근 천연물 hydrangenol을 활용한 건강기능식품 개발에서 사용가능 재료의 한계를 설정하기 위한 산수국과 수국에 대한 종 설정 연구도 요구되고 있다. 따라서 본 연구에서는 nrDNA ITS, trnL-F, trnC-ycf6의 3개 구간의 DNA sequence와 NCBI (National Center for Biotechnology Information)를 참고한 분자생물학적 분석결과와 외부형태학적 재검토 결과를 통하여 H. marcrophylla를 종(Species)으로서의 분류학적 위치 지지하며 또한 수국과 산수국을 가까운 유연관계를 가지는 독립된 분류군으로 분류한 의견을 지지하는 결과를 도출하였다. 향후 hydrangenol 활용의 기초정보로 활용되기를 기대한다.

• Korean Journal of Plant Resources
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• v.27 no.5
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• pp.534-539
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• 2014

This study was carried out to establish seed propagation method of Hydrangea petiolaris Siebold & Zucc., one of the specific and protected plant species for the floral region. Seed size ranged $1.36{\times}0.84mm$, weight of thousand seeds was $2.3{\pm}0.02mg$. Observation of seed morphology characters showed wrinkled surface and elliptical shape, indicating amber color and fine seeds. Moisture contents of seeds, increased rapidly by water-soaking treatment, recording maximum moisture contents (31.9%) after 48 hours. Percent germination of seeds was higher under the light than dark condition. Seed germination was the best at $25^{\circ}C$ under the light condition (78.0%) among temperature and light conditions treated. Percent germination, germination energy and $T_{50}$ was greatly improved by soaking in $GA_3$ solutions for 48 hours. Especially, $100{\sim}500mg{\cdot}L^{-1}$ $GA_3$ treatment resulted in the highest germination rate as 90.0% and above.

• Research in Plant Disease
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• v.7 no.1
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• pp.1-7
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• 2001

An isolate of Cucumber mosaic cucumovirus(CMV) was isolated from Hydrangea macrophylla for. otaksa(Sieb. et Zucc. ) Wils. showing mosaic symptoms, and designated as Hm-CMV. Hm-CMV was characterized by the tests of host range, physical properties, serological properties, RNA and coat protein compositions, and reverse transcription and polymerase chain reaction (RT-PCR) analysis. Twelve species in 4 families were used in the host range test of Hm-CMV and could be differentiated from Y-CMV used as a control CMV by the ringspot and line pattern on inoculated leaves of several tobacco plants. Thevirus produced local lesions on inoculated leaves of Chenopodium amarticolor, C. quinoa and Vigna unguiculata. The physical properties of the virus were as follows; thermal inactivation point(TIP) was 60$\^{C}$, dilution end point (DEP) was 10$\^$-3/, and longevity in vitro (LIP) was 3∼4 days. Hm-CMV was serologically identical to Y-CMV. SDS-polyaciylamide gel electrophoresis(SDS-PAGE) showed one major protein band of about 28 kDa. In RNA or dsRNA analysis, Hm-CMV consisted of four RNA or dsRNA species, but satellite RNA was not detected. In RT-PCR using CMV-common primer and CMV subgroup I-specific primer, bothe amplified expected size of about 490 bp and 200 bp DNA fragments from Hm-CMV, respectively. Restriction enzyme analysis of the 490 bp RT-PCR products using EcoR I and Msp I showed that Hm-CMV belonged to CMV subgroup I. However, Hm-CMV could be differentiated from other CMV subgroup I isolates by RNA fingerprinting by arbitrarily primed polymerase chain reaction (RAP-PCR).