Korean Journal of Plant Taxonomy (식물분류학회지)

The Korean Society of Plant Taxonomists (한국식물분류학회)
권호 표지
DOI QR코드

DOI QR Code

Isolation and characterization of microsatellite markers for Hydrangea luteovenosa (Hydrangeaceae), an endangered species in Korea

한국 제주도에 자생하는 멸종위기종 성널수국(수국과)의 microsatellite 분자마커 개발

  • Ito, Takuya (Graduate School of Agriculture, Kyoto University) ;
  • Kaneko, Shingo (Department of Symbiotic Systems Science, Fukushima University) ;
  • Yokogawa, Masashi (Laboratory of Botany, Osaka Museum of Natural History) ;
  • Song, Gwan-Pil (Research Institute for Environmental Resources of Jeju) ;
  • Choi, Hyeok-Jae (Department of Biology, Changwon National University) ;
  • Isagi, Yuji (Graduate School of Agriculture, Kyoto University)
  • Received : 2013.01.11
  • Accepted : 2013.03.11
  • Published : 2013.03.29
Download PDF
⟨ Previous Next ⟩

Abstract

Hydrangea luteovenosa is a critically endangered plant species of Jeju Island in Korea, though it is widely distributed in western Japan. We isolated and characterized five microsatellite loci in this species. The number of alleles ranged from 3 to 27, observed heterozygosity from 0.27 to 0.86, and expected heterozygosity from 0.34 to 0.91. The markers described here will be useful for investigating the genetic diversity, genetic structure, and gene flow of H. luteovenosa, and the genetic findings would contribute to the establishment of effective conservation measures for this species in Korea.

성널수국은 일본 관서지방에 널리 분포하고 있지만, 한반도에서는 제주도에서만 발견되고 있는 멸종위기 식물이다. 본 연구에서는 성널수국에서 총 5개의 microsatellite 유전자좌를 형질화하여 마커를 개발하였다. 유전자좌당 대립유전자수의 범위는 3-27개, 이형접합률의 관측치와 기대치는 각각 0.27-0.86과 0.34-0.91로 나타났다. 본 연구에서 개발된 microsatellite 마커는 성널수국의 유전적 다양성 및 구조, 그리고 유전자 이동을 밝히는데 사용될 수 있을 것이며, 그 결과들은 한국산 성널수국에 대한 효과적인 보전 전략을 제시하는데 일조할 것으로 기대된다.

Keywords

References

  1. Frankham, R. 1995. Conservation genetics. Annual Review of Genetics 29: 305-327. https://doi.org/10.1146/annurev.ge.29.120195.001513
  2. Goudet, J. 1995. FSTAT (version 1.2): a computer program to calculate F-statistics. Journal of Heredity 86: 485-486. https://doi.org/10.1093/oxfordjournals.jhered.a111627
  3. Guichoux, E., L. Lagache, S. Wagner, P. Chaumeil, P. Leger, O. Lepais, C. Lepoittevin, T. Malausa, E. Revardel, F. Salin and R. J. Petit. 2011. Current trends in microsatellite genotyping. Molecular Ecology Resources 11: 591-611. https://doi.org/10.1111/j.1755-0998.2011.03014.x
  4. Izuno, A., M. Takamiya, S. Kaneko and Y. Isagi. 2012. Genetic variation and structure of the endangered Lady Fern Athyrium viridescentipes based on ubiquitous genotyping. Journal of Plant Research 125: 613-618. https://doi.org/10.1007/s10265-012-0482-x
  5. Kim, C. S. 2009. Vascular plant diversity of Jeju Island, Korea. Korean Journal of Plant Resources 22: 558-570.
  6. Lian, C., M. A. Wadud, Q. Geng, K. Shimatani and T. Hogetu. 2006. An improved technique for isolating codominant com-pound microsatellite markers. Journal of Plant Research 119: 415-417. https://doi.org/10.1007/s10265-006-0274-2
  7. Lian, C. and T. Hogetsu. 2002. Development of microsatellite markers in black locust (Robinia pseudoacasia) using a dualsupperession-PCR technique. Molecular Ecology Notes 2: 211-213.
  8. Milligan, B. 1992. Plant DNA isolation. In Molecular genetic analysis of populations: a practical approach. Hoelzel, A. R. (ed.), IRL Press, Oxford, Pp. 59-88.
  9. Moon, M. O., Y. J. Kang, C. H. Kim and C. S. Kim CS. 2004. An unrecorded species in Korea flora: Hydrangea luteovenosa Koidz. (Hydrangeaceae). Korean Journal of Plant Taxonomy 34: 1-7. (in Korean)
  10. Rozen, S. and H. Skaletsky. 2000. Primer3 on the web WWW for general users and for biologist programmers. In Bioinformatics methods and protocols: methods in molecular biology. Krawetz, S. and S. Misener (eds.), Humana Press, Totowa, Pp. 365-386.
  11. Schwatrz, M. K., G. Luikart and R. S. Waples. 2007. Genetic monitoring as a promising tool for conservation and management. Trends in Ecology & Evolution 22(1): 25-33. https://doi.org/10.1016/j.tree.2006.08.009
  12. Yun, Y. E., J. N. Yu, B. Y. Lee and M. H. Kwak. 2011. An introduction to microsatellite development and analysis. Korean Journal of Plant Taxonomy 41: 299-314. (in Korean)

Cited by

  1. Population and genetic status of a critically endangered species in Korea: Hydrangea luteovenosa (Hydrangeaceae) vol.47, pp.1, 2017, https://doi.org/10.11110/kjpt.2017.47.1.1