• Journal of the Korean Society for Aeronautical & Space Sciences
• /
• v.30 no.2
• /
• pp.59-66
• /
• 2002

In this paper, we present the simulataneous monitoring of the strain and temperature during cures f various composite laminates using fiber Bragg grating/extrinsic Fabry-Perot interferometric (FBG/EFPI) hybrid sensors. Three types of graphite/epoxy composite were used : a undirectional laminate, a symmetric cross-ply laminate, and a fabric laminate. Two FBG/EFPI hybrid sensors were embedded in each laminate at different directions and different locations. We performed the real time monitoring of fabrication strains and temperatures at two points within the composite laminates during cure process in an autoclave. Throuhg these experiments, FBG/EFPI sensors proved to be an efficient choice for smart cure monitoring of composite structures.

• Journal of Microbiology and Biotechnology
• /
• v.15 no.5
• /
• pp.1039-1046
• /
• 2005

The salt resistance of antibacterial activity and synergistic effect with clinically used antibiotic agents are critical factors in developing effective peptide antibiotic drugs. For this reason, we investigated the resistance of antibacterial activity to antagonism induced by NaCl and $MgCl_2$ and the synergistic effect of P20 with cefotaxime. P20 is a 20-residue synthetic peptide derived from a cecropin A (CA)-melittin(ME) hybrid peptide. In this study, P20 was found to have potent antibacterial activity against clinically isolated methicillin-resistant Staphylococcus aureus (MRSA) strains without hemolytic activity against human erythrocytes. The combination study revealed that P20 in combination with cefotaxime showed synergistic antibacterial activity in an energy-dependent manner. We also confirmed the synergism between P20 and cefotaxime by fluorescence-activated flow cytometric analysis by staining bacterial cells with propidium iodide (PI) and bis-(1,3-dibutylbarbituric acid) trimethine oxonol (BOX). This study suggests that P20 may be useful as a therapeutic antibiotic peptide with synergistic effect in combination with conventional antibiotic agents.

• Journal of Environmental Science International
• /
• v.7 no.5
• /
• pp.599-605
• /
• 1998

The purpose of this work was to examine whether X-Y chromosome dissociation in the primary spermatocytes of mice could be used as an in vivo short-term assaying system that detect environmental mutagens. Four alkylating agents(EMS, MMS, MMC and MNNG) which were known as strong mutagens were administered to BALB/c male mice 3-4 months old. In the control group, the mean frequencies of previously dissociated X and Y chromosomes and autosomes were 7.17% and 2.12%, respectively. Compared to the control group, mutagen-treated groups have no significant differences in dissociation rate of autosomes, while these poops were about 1.2-2.5 times higher in the frequencies of X-Y dissociation. Generally, X-Y dissociation frequency increased consistently with the concentration of mutagens whereas the tendency of autosome dissociation frequency was variable among several mutagens. These results suggest that X-Y dissociation in the primary spermatocytes of mice is applicable as an vivo short-term assaying system for environmental mutagens. There were significantly distinct increase in dissociation of X-Y chromosome in both the hybrid and parents but the X-Y previous dissociation of hybrid appeared higher frequency than BALB /c and wild mice. These results indicate that the factor related to binding X-Y chromosome is specific to strains.

• Journal of Mushroom
• /
• v.16 no.3
• /
• pp.140-146
• /
• 2018

Pleurotus eryngii is one of the most commercially important mushrooms cultivated in Korea. However, the shelf-life of the fruiting body is short, limiting its export. A new hybrid strain H17 of P. eryngii was developed to extend the shelf-life by mono-mono crossing between monokaryotic strains derived from DanBi and KNR2774. Although the cultivation period of H17 was slightly longer than that of the reference cultivar Kenneutari No.2, the quality did not change and remained normal after a period of 65.0 days at $4^{\circ}C$. This result was significantly different from that of the reference cultivar Kenneutari No.2. Analysis of the genetic characteristics of the new hybrid strain H17 revealed a different profile from that of the parental and reference cultivars when random amplification of polymorphic DNA (RAPD) primers was used. These results demonstrate that H17 is a new cultivar with improved storability after harvesting.

• Journal of Microbiology and Biotechnology
• /
• v.30 no.5
• /
• pp.770-776
• /
• 2020

Genistein is a type of isoflavonoid found predominantly in leguminous plants. Genistein has diverse biological activities, such as anthelmintic and antioxidant effects, as well as inhibitory effects on the growth of several cancers. In addition, genistein is well known as a phytoestrogen. In this study, we attempted to biologically synthesize genistein from either p-coumaric acid or naringenin using Escherichia coli as a biotransformation host. Four genes, Os4CL, PeCHS, RcIFS, and OsCPR, were used for genistein production. To functionally express RcIFS and OsCPR, two members of the cytochrome P450 family, in E. coli, the membrane-binding anchor domain of each gene was removed, and RcIFS and OsCPR were translationally fused to generate an RcIFS-OsCPR hybrid. Os4CL and PeCHS, or the RcIFS-OsCPR hybrid, were then transformed into E. coli BL21(DE3). Using these strains, we optimized our culture system at a laboratory scale in terms of the cell density, concentrations of substrate and isopropyl-β-D-thiogalactoside, temperature, and culture medium. Under the optimized culture conditions, genistein was produced at up to 35 mg/l and 18.6 mg/l using naringenin and p-coumaric acid, respectively.

• International Journal of Industrial Entomology and Biomaterials
• /
• v.2 no.2
• /
• pp.129-132
• /
• 2001

Biometabolic assessment was made in early and late embryonic stage (just before hatching) of one poly, one bi and their hybrids (DP, YPe, DP ${\times} Ype, and YPe ${\times} DP respectively ) of Bombyx mori to observe the racial differences. Protein and nucleic acid (RNA and DNA) concentrations were recorded to be significantly higher in bivoltine breed (YPe) and also in the hybrid than the polyvoltine (DP) strain in both the stages of embryonic development. The single egg weight of polyvoltine race was lower as compared to that of bivoltine and the hybrid studied. Age specific changes in all the biomolecules were evident where protein and RNA concentrations were elevated sharply in prehatched larvae while in case of DNA it was observed to be just reverse. The differences in protein, RNA and DNA composition between breeds and hybrids reflect the racial variations in biometabolic demands responsible for differential growth and development of the breeds and hybrids.

• 한국지구물리탐사학회:학술대회논문집
• /
• 2007.06a
• /
• pp.251-256
• /
• 2007

The aim of this paper is development of resistivity seismic dilatometer (RSDMT) system. The resistivity module for obtaining apparent resistivity depth plot and seismic module for obtaining shear wave velocity (Vs) depth plot are attached to the conventional flat dilatometer testing equipment. From shear wave velocity profile, the stiffness at low strains of a site can be evaluated in undisturbed condition. And the resistivity value contains some information about water content and mineral characteristics of clayey soil. Specially manufactured resistivity and seismic modules were connected between commercialized DMT blade and drilling rod. To enhance reliability and repeatability of RSDMT test, automatic testing system including notebook based data acquisition system and automatic surface source system were developed. RSDMT system can be performed rapidly and can obtaine more reliable data at the same point compared with the separated testing system. The verification studies for the developed RSDMT system are going to be performed. From these studies, the effectiveness of integrated hybrid testing system will be checked in light of proper evaluation of geotechnical design parameters of clayey soils.

• Steel and Composite Structures
• /
• v.38 no.6
• /
• pp.691-703
• /
• 2021

In this study, experiments were conducted to evaluate the flexural performance of prestressed hybrid wide flange (PHWF) beams with hollowed steel webs. A total of four PHWF beams were fabricated, where the width and spacing of the steel webs and the presence of cast-in-place (CIP) concrete were set as the main test parameters, and their flexural behavior and crack patterns, and the longitudinal strain distribution in a section with respect to the width and spacing of the steel webs were analyzed in detail. The experiment results showed that, as the ratio of the width to the spacing of the steel webs decreased, the flexural stiffness and strength of the PHWF beams without CIP concrete decreased. In addition, in the case of composite PHWF beam with CIP concrete, fully composite behavior between the precast concrete and the CIP concrete was achieved through the embedded steel member. Finite element analyses were performed for the PHWF beams considering the bond properties between the hollowed steel webs and concrete, and nonlinear flexural analyses were also conducted reflecting the pre-compressive strains introduced only into the bottom flange. From the comparison of the test and analysis results, it was confirmed that the analysis models proposed in this study well evaluated the flexural behavior of PHWF beams with and without CIP concrete.

• Journal of Microbiology and Biotechnology
• /
• v.13 no.6
• /
• pp.976-983
• /
• 2003

Seven Escherichia coli cells defective with aerobic growth were isolated by the insertion of ${\lambda}placMu53$, a hybrid bacteriophage of ${\lambda}$ and Mu, which created a transcriptional fusion to lacZY. These insertion mutant cells were tested on an XG ($5-bromo-4-chloro-3-indolyl-{\beta}-D-galactopyranoside$) medium for anaerobic expression of lacZ by fusion to a promoter. The chromosomal DNA from these strains were digested by EcoRI, and the EcoRI fragments that contained the fused gene and lacZ sequence were identified by Southern hybridization, using lacZ containing plasmid as a probe. The EcoRI fragment from each strain was cloned and sequenced. The sequence data were compared with the GenBank database. The mutated gene of three strains, CYT4, CYT5, and OS11, was found to be identical, and it was nrdAB that encoded ribonucleoside diphosphate reductase. The gene nrdAB was at min 50.5 on the Escherichia coli linkage map and 2,348,084 on the physical map, and is involved in hemAe-related reduction-oxidation reaction. OS6 and OS14 mutant strains had insertion at min 8.3 and the mutated gene was hemB. The hemB encodes 5-aminolevulinate dehydratase or porphobilinogen synthase. The OS3 mutant had insertion in cydB at min 16.6. The cydAB encodes cytochrome d oxidase. In the case of OS1, the fusion was made with sucA, the E1 component of ${\alpha}-ketoglutarate$ dehydrogenase.

• Korean journal of applied entomology
• /
• v.26 no.3 s.72
• /
• pp.165-170
• /
• 1987

This experiment was carried out to evaluate the difference in the biochemical characteristic of the brown planthoppers of the insecticide risistant, susceptible strains and their hybrid progenies. Activity of the esterase isozyme separated by electrophoresis method was remarkably high in the resistant strain as compared with the susceptible strain. Esterase activity between the insecticide-treated strains and the non-insecticide strains was not degraded in the resistant strain and the $F_1$, but remarkably degraded in the susceptible strain. The increase of esterase activity was associated with the development of resistance, and that was inherited with a dominant gene.