Journal of Environmental Science International (한국환경과학회지)
- Volume 7 Issue 5
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- Pages.599-605
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- 1998
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- 1225-4517(pISSN)
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- 2287-3503(eISSN)
Studies on X-Y Chromosome Dissociation Induced by Environmental Mutagens in Mouse
환경성 돌연변이원에 의한 Mouse의 X-Y 염색체 조기분리에 관한 연구
Abstract
The purpose of this work was to examine whether X-Y chromosome dissociation in the primary spermatocytes of mice could be used as an in vivo short-term assaying system that detect environmental mutagens. Four alkylating agents(EMS, MMS, MMC and MNNG) which were known as strong mutagens were administered to BALB/c male mice 3-4 months old. In the control group, the mean frequencies of previously dissociated X and Y chromosomes and autosomes were 7.17% and 2.12%, respectively. Compared to the control group, mutagen-treated groups have no significant differences in dissociation rate of autosomes, while these poops were about 1.2-2.5 times higher in the frequencies of X-Y dissociation. Generally, X-Y dissociation frequency increased consistently with the concentration of mutagens whereas the tendency of autosome dissociation frequency was variable among several mutagens. These results suggest that X-Y dissociation in the primary spermatocytes of mice is applicable as an vivo short-term assaying system for environmental mutagens. There were significantly distinct increase in dissociation of X-Y chromosome in both the hybrid and parents but the X-Y previous dissociation of hybrid appeared higher frequency than BALB /c and wild mice. These results indicate that the factor related to binding X-Y chromosome is specific to strains.
Keywords
- X-Y chromosome dissociation;
- environmental mutagens;
- BACB/c;
- wild mice;
- in vivo short-term assaying system