• Title/Summary/Keyword: Hybrid strains

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Analysis of the Effect of Media Types and Chromagenic Chemicals on the Detection of Extracellular Laccase Activity among Lentinula edodes Strains (표고 교잡균주들의 세포외 laccase 활성 검출에 미치는 배지성상과 발색반응 시약의 영향 분석)

  • Kim, Jun-Young;Kwon, Hyuk-Woo;Tang, Longqing;Ko, Han-Kyu;Kim, Seong-Hwan
    • The Korean Journal of Mycology
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    • v.39 no.1
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    • pp.48-52
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    • 2011
  • Breeding of Lentinula edodes generates a number of hybrid strains that are subject to evaluation for good traits for the mushroom production. As an effort to understand biochemical properties of the hybrid strains, this study tried to develop a fast and easy method for comparison of the ability of producing extracellular laccase among hybrid strains of Lentinula edodes. For this aim, we estimated the effect of media types and chromagenic chemicals on the detection of extracellular laccase in seven hybrid strains of L. edodes. When Remazol Brilliant Blue R (RBBR) dye was used for chromagenic reaction, the detection of the enzyme activity was feasible both in the solid and liquid media containing not potato dextrose but malt extract as a nutrient component. When guaiacol was used for chromagenic reaction, the detection of the enzyme activity was feasible both in the solid and liquid media containing either potato dextrose or malt extract as a nutrient component. Malt extract-based liquid culture with RBBR or guaiacol in 2 ml microfuge tube allowed us to economically and quantitatively detect and compare the enzyme activity within 3 days among the tested hybrid strains of L. edodes.

Development of a Composite Rotor for Flywheel Energy Storage System (플라이휠 에너지 저장 장치용 복합재 로터 개발)

  • Kim, Myung-Hun;Han, Hun-Hee;Kim, Jae-Hyuk;Kim, Seong-Jong;Ha, Seong-Gyu
    • Proceedings of the Korean Society For Composite Materials Conference
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    • 2005.11a
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    • pp.169-172
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    • 2005
  • A flywheel system is an electromechanical energy storage device that stores energy by rotating a rotor. The rotating part, supported by magnetic bearings, consists of the metallic shaft, composite rims of fiber-reinforced materials, and a hub that connects the rotor to the shaft. The delamination in the fiber wound composite rotor often lowered the performance of the flywheel energy storage system. In this work, an advanced hybrid composite rotor with a split hub was designed to both overcome the delamination problem in composite rim and prevent separation between composite rim and metallic shaft within all range of rotational speed. It was analyzed using a three-dimensional finite clement method. In order to demonstrate the predominant perfom1ance of the hybrid composite rotor with a split hub, a high spin test was performed up to 40,000 rpm. Four radial strains and another four circumferential strains were measured using a wireless telemetry system. These measured strains were in excellent agreement with the FE analysis. Most importantly, the radial strains were reduced using the hybrid composite rotor with a split hub, and all of them were compressive. As a conclusion, a compressive pressure on the inner surface of the proposed flywheel rotor was achieved, and it can lower the radial stresses within the composite rotor, enhancing the performance of the flywheel rotor.

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Breeding and Screening of Lentinula edodes Strains Resistant to Trichoderma spp.

  • Lee, Hye-Min;Bak, Won-Chull;Lee, Bong-Hun;Park, Hyun;Ka, Kang-Hyeon
    • Mycobiology
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    • v.36 no.4
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    • pp.270-273
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    • 2008
  • Trichoderma spp. cause large crop losses of the cultivated shiitake mushroom, Lentinula edodes. We bred several shiitake strains that are resistant to Trichoderma spp. using di-mon mating to establish a useful method for controlling the greenmold disease. We examined the competitive ability of L. edodes against Trichoderma spp. using a dual culture system to select resistant strains. By screening Trichoderma-resistant strains, we found that among 11 parental strains, 4 strains, including KFRI 36, were confirmed resistant strains. They showed especially strong resistance to T. harzianum, which formed deadlock after mycelial contact and then invaded into the territory of T. harzianum. KFRI 171 also showed resistance to T. atroviride strains. Among 13 strains, which were made by hybridization of shiitake strains, 5 were confirmed to be resistant to Trichoderma, including KFRI 58-1. Their resistance was not correlated to the resistant activity of their parents’ strains. Two strains lose resistance and two strains acquire resistance compared to their parents’ strains. In SEM observation, the mycelium of L. edodes at the interaction zone of Lentinula-Trichoderma was rugged and swollen by T. harzianum.

Development of Versatile Strains of Pseudomonas Degrading Various Persistent Aromatic Hydrocarbons (다양한 난분해성 방향족 탄화수소를 분해하는 Pseudomonas의 균주개발)

  • 이지현;최인성;박경량;박용근;이영록
    • Korean Journal of Microbiology
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    • v.28 no.3
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    • pp.236-242
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    • 1990
  • To develop the new strains of microorganisms having the degradative ability for various aromatic hydrocarbons, the hybrid plasmid pKG2 having the 2,4-Dichlorophenoxyacetic acid(2,4-D) degradative genes, the hybrid plasmid pKG3 containg the naphthalene degradative genes and TOL plasmid were introduced into Pseudomonas putida KUD 12 and P. putida KUP 10 by transformation or conjugation which originally have the degradative ability of the synthetic surfactants and phthalate esters, respectively. From P. putida KUD12, the new strains of P. putida KUD101(pKG2), KUD102(pKG3), KUD103(TOL), and KUD202(pKG3, TOL) were obtained, and KUD106(pKG2), KUD107(pKG3), KUD108(TOL) were originated from the P.putida KUP10. The degradative abilities in P. putida KUD101, KUD102 and KUD107 were similar with those of the original strains. The P. putida KUD103, KUD106 and KUD202 had a little lower and P. putida KUD108 had a better degradative abilitie than those of the original ones. In the case of mixed cultures, the mixed culture of KUD107 and KUD108 had a better degradative abilities than those of the other mixed cultures.

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Screening Resistance to Crown Gall Disease by Pathogen Inoculation in Muscadine and Florida Hybrid Grapes (병원균 접종에 의한 Muscadine 및 플로리다 교잡종 포도의 줄기혹병 저항성 검정)

  • Noh, Jung-Ho;Park, Kyo-Sun;Lu, Jiang;Yun, Hae-Keun
    • Korean Journal of Organic Agriculture
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    • v.23 no.3
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    • pp.497-507
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    • 2015
  • It is necessary to evaluate the resistance to disease among genetic resources for development of disease resistant grapes. This study was conducted to screen the resistance to crown gall in muscadine and Florida hybrid bunch grapes by pathogen inoculation. In order to compare the responses to infection with different pathogen strains, muscadine and Florida hybrid grapes were inoculated with 3 strains of Agrobacterium vitis. Although there were different levels crown gall formation among grape cultivars, there little variation in response to inoculated strains. Among 29 muscadine cultivars tested by inoculation of A. vitis 'C4612', most of them were shown to be susceptible, and 'Gold Isle' and 'Africa Queen' were highly susceptible, and two cultivars, 'Welder' and 'Jumbo' were found to be resistant to crown gall disease. Among Florida hybrid grapes, 'Daytona', 'Stover', and 'Swanee' were susceptible and 'Blanc du Bois' was moderately susceptible to crown gall. Because muscadine grapes have been actively utilized as useful genetic resources for development of new grape varieties by intersub-genus cross, this result from the screening of resistance among muscadine grapes can provide valuable information in breeding programs of grape resistant to crown gall.

Selective Breeding and Hybridization of Lentinus edodes Strains for Bed-log Cultivation (선발육종(選拔育種) 및 교잡육종(交雜育種)에 의한 원목재배용(原木栽培用) 표고균주(菌株) 육성(育成))

  • Bak, Won Chull;Lee, Tai Soo;Lee, Won Kyu;Byun, Byung Ho;Yi, Chang Keun
    • Journal of Korean Society of Forest Science
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    • v.85 no.2
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    • pp.309-315
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    • 1996
  • Selective breeding and hybridization were attempted to improve shiitake(Lentinus edodes) strains for bed-log cultivation, and the results obtained are as follows. 1. Thirty five strains collected in Korea and abroad were cultivated for one generation(five years) and fruit body yields were estimated by the amounts produced from $1m^3$ of oak tree(Quercus mongolica) logs. Thus, three high-temperature type strains including FRI 177 showing productivity of $139kg/m^3log$, two mid-temperature type strains including FRI 188 of $120kg/m^3log$, and seven low-temperature type strains including FRI 187 of $96kg/m^3log$ were selected as superior strains. 2. Twelve hybrid strains confirmed as independent strains through indoor-test with hybrid strains made by Di-mon mating method were cultivated for five years(one generation). Among them, FRI 182 and FRI 184 were turned out to be superior strains. FRI 182 of high-temperature type showed the yield of 108kg per $1m^3logs$ and FRI 184 of low-temperature type produced $103kg/m^3log$. 3. There were no significant differences in productivity among high-, mid- and low-temperature types, but some differences appeared among strain groups. The correlation was not shown in productivities between bed-log and sawdust cultivations.

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Characteristics and breeding of a new oyster mushroom (Pleurotus ostreatus) variety 'Chunhwashim' by using cytoplasmic hybrid (세포질전환 기법에 의한 신품종 느타리 '천화심'의 육성 및 자실체 특성)

  • Shin, Pyung-Gyun;Yoo, Young-Bok;Kong, Won-Sik;Oh, Youn-Lee
    • Journal of Mushroom
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    • v.13 no.2
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    • pp.129-134
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    • 2015
  • Hybrid strains was selected by crossing between dikaryotic strain ASI 0628 (Dagul) bred from ASI 2596 (Suhan No.3) and ASI 2782(Black pileus mutant) and monokaryotic strain ASI 2344-84. The strain 84 that shown the best cultural characteristics was selected to be a new cytoplasmic hybrid variety and named as 'Chunhwashim'. The 'Chunhwashim' shown incompatibility among them forming the confrontation growth line against parental strains ASI 0628 (Dagul) and ASI 2344 (chunchu 2ho). Fruiting body produced about $161.4{\pm}4.8g$ per bottle. And also the individual generation of fruit body is multiple than chunchu2ho as 40.9. The 'Chunhwashim' was cytoplasmic hybrid included hybrid DNA bands of parental strains ASI 0628 (Dagul) and ASI 2344 (chunchu 2ho) by Random Amplified Polymorphic DNA (RAPD) primer, and mitochondria DNA band of monokaryotic ASI 2344 (chunchu 2ho) using mitochondria microsatellite DNA marker. This new cytoplasmic hybrid variety 'Chunhwashim' of oyster mushroom is characterized by multiple of individual generation and deeply grey color of pileus.

Effect of Water Temperature and Photoperiod on the Oxygen Consumption of Four Different Strains of Red Seabream, Pagrus major

  • Oh, Sung-Yong;Choi, Hee Jung;Kim, Min-Suk;Park, Yong Joo;Myoung, Jung-Goo;Kwon, Joon Yeong;Choi, Cheol Young
    • Journal of Marine Life Science
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    • v.1 no.2
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    • pp.109-116
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    • 2016
  • We determined the effects of different water temperatures (15, 20, and 25℃) and photoperiod cycles (24L:0D, 12L:12D, and 0L:24D) on the oxygen consumption of the offspring of a cultured Japanese strain (JJ), a selected Korean strain (KK), and intraspecific hybrid strains (JK and KJ) of red seabream, Pagrus major, under starvation conditions. The different fish strains, water temperatures, and photoperiod cycles had effects on the mean oxygen consumption of fish. Oxygen consumption increased with increasing water temperatures for all photoperiod treatments (p<0.001). Fish held in continuous darkness (0L:24D) used consistently less oxygen than fish exposed to continuous light (p<0.05). The oxygen consumption of fish exposed to the light phase in a 12L:12D photoperiod was higher than that of fish in the dark phase of the 12L:12D cycle, and differences were significant in three of the strains: JJ (15℃), KK (15 and 20℃), and KJ (25℃). The oxygen consumption of the inbred (JJ and KK) and intraspecific hybrid (JK and KJ) strains varied with differing water temperatures and photoperiod cycles. The JK strain displayed significantly higher oxygen consumption than the other strains under all experimental conditions except 15℃ with a 0L:24D photoperiod. The JK and KJ strains usually showed the highest and lowest oxygen consumption values, respectively, whereas the inbred strains exhibited intermediate values. Oxygen consumption in the JJ and JK strains was usually higher than that of the KK and KJ strains. We propose that differences in the thermal sensitivity and photosensitization properties of the strains contribute to differences in their ability to adapt to changes in water temperature and photoperiod, thus resulting in differences in the amplitude of their metabolic rates.

Production of Amylase from Intergeneric Hybrids between 'Aspergillus oryzae var oryzae and Penicillium Verruculosum F-3 by Nuclear Transfer (핵전이에 대한 Aspergillus oryzae var oryzae와 Penicillium verruculosum F-3의 잡종으로부터 Amylase 생산)

  • Yang, Young-Ki;Park, Yeol;Kim, sung-Joon;Moon, Myung-Nim;Park, Hyung-Nam;Lim, Chae-Young;Rhee, Young-Ha
    • Microbiology and Biotechnology Letters
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    • v.22 no.1
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    • pp.23-30
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    • 1994
  • Intergeneric hybrids formed between Aspergillus oryzae var oryzae and Penicillium verruculosum F-3 were obtained by nuclear transfer technique. Several auxotrophic mutants isolated fromconidio-spores of the two strains mutagenized with ultraviolet and N-methyl-N-nitrosoguanidine. Optimal conditions for formation of intergeneric hybrids were investigated. Frequencies of hybrid formation by nuclear transfer were 3$\times $10$^{-5}$ $~$1$\times $10$^{-5}$. From observation of genetic stability, conidial size, DNA content, nuclear stain, it was suggested that their karyptypes are aneuploid. The hybrid posses the 1.3$~$2.2 fold higher amylase activities than those of parental strains. It was also revealed that some hybids had different isozyme patterns compared to those of parental strains by amylase activity assays.

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RAPD Analysis and Cordycepin Concentration of Hybrided Cordyceps militaris Strains by Mating (교잡된 Cordyceps militalis 균주의 RAPD 분석 및 생리활성물질인 cordycepin 함량 측정)

  • Jeong, Jin-Woo;Jin, Cheng-Yun;Kim, Mun-Ock;Lee, Jae-Yun;Choi, Yung-Hyun;Lee, Jae-Dong
    • The Korean Journal of Mycology
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    • v.37 no.1
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    • pp.86-90
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    • 2009
  • The C. militaris belongs to entomopathogenic fungi, which have their specific sequences in internal transcribed spacer regions (ITS1 and ITS2) depending on species. In this study, to identify the phylogenetic relationship of the strain hybrided by mating of C. militaris, we compared DNA sequences of ITS regions and 5.8S ribosomal DNA (rDNA) repeat unit of hybrid strain and its parental strains. The result revealed that hybrid strains are C. militaris species. In addition, cordycepins produced by hybrid strains and other strains of C. militaris were analyzed by HPLC with 20mM $KH_2PO_4$ of mobile phase and C-18 columns. The result indicated that the strain hybrided by mating produce higher concentration of phytochemical cordycepin than other C. militaris strains.