• 한국균학회지
• /
• 제37권1호
• /
• pp.65-72
• /
• 2009

톱밥배지에 적합한 품종 구비 조건으로서 요구되는 신규성, 구별성, 균일성, 안정성에 대해 교잡균주제조에 따른 균사생장, 배양일수, 생산성, 온도형 등을 비교하여 톱밥배지에 적합한 균주육종을 시도하였다. 본 실험에서는 Di-mon교배법을 이용하여 이핵 균주 21개와 일핵 균주 2개 사이의 상호교배에서 19개의 새로운 균주를 만들었다. 이 가운데 균사생장과 목재 부후력이 활발한 10개 균주를 선발하였다. 이 선발된 균주들은 100일, 125일, 150일 등으로 배양일수를 달리하여 생산성 검정을 하였다. 그 결과 100일 처리에서 KFRI 960, 961, 962, 963, 964, 971, 973균주가 생산성이 우수하였으며, 125일 처리에서 KFRI 968, 970균주가 생산성이 우수하였다. 배양일수에 따른 생산성은 100일과 150일 처리사이에서 차이가 났다. 교배 온도형의 비교는 고온성 사이의 교배와 저온성과 고온성 사이의 교배, 아직 결정되지 않은 온도형과 고온성 간의 교배의 생산성차이는 없었으며, 중온성과 고온성과의 교배로 만들어진 균주들과는 생산성 차이가 났다. 교잡균주들의 온도형 분석은 고온성 균주가 다른 온도형에 비해 우성형질을 가졌다.

• 한국버섯학회지
• /
• 제13권1호
• /
• pp.41-49
• /
• 2015

표고(Lentinula edodes)는 우리나라의 주요 임산버섯으로 최근 표고 톱밥재배가 정착됨에 따라 급속도로 확산되고 있으나 국내 환경에 적합하고 재배시기를 다변화 할 수 있는 균주의 육성이 시급하다. 따라서 9개의 균주를 시험재배하여 생산성이 높고 자실체 특성이 우수한 4개 균주(SANJO701HO, FMRI2534, FMRI2337, FMRI2613)를 모균주로 선발하였고 균주당 각각 20개씩 총 80개의 단핵균주를 분리하여 117개의 교배균주를 육성하였다. 그중 산조701호와 FMRI2337의 교배조합이 다른 조합의 교배균주들보다 평균생장속도가 10% 가량 낮았으며 이들 교배균주의 배양 및 생리적 특성을 비교 조사하였다. 따라서 본 연구는 우수한 톱밥재배용 품종개발의 기반조성을 마련하고자 하였으며, 차후 교배균주에 대한 생산성 검정의 연구가 필요하다.

• 한국버섯학회지
• /
• 제13권4호
• /
• pp.294-300
• /
• 2015

실내에서 육성된 117개 교배균주를 시험 재배하여 자실체의 특성 및 생산성을 검정하고 결과가 우수한 07-55, 07-66, 07-84, 07-93, 07-117균주를 선발하고 이들의 모균주들과 확대 재배하여 모균주와의 생산성을 비교한 결과 각 균주의 생산성은 07-66균주가 36.1%의 회수율을 보여 가장 높게 나타났으며 07-117균주는 35.6%, 07-93균주는 27.1%, 08-84균주는 25.7%로 조사되었고 07-55균주는 가장 생산성이 낮은 6.5%로 조사되었다. 07-93 균주와 07-66균주는 생산성이 높으나 대가 긴 특성을 보였고 07-117균주는 전체적인 자실체 품질이 다른 균주들보다 낮았다. 또한 모균주와 교배균주간에 재배를 통한 온도형 분석 결과 07-117균주는 고온형, 07-66 및 07-84균주는 중온형, 07-55 및 07-93균주는 저온형으로 구분되었으며 07-93균주는 비교적 버섯발생온도범위가 넓은 가능한 균주이나 품질이 저조하였다.

• 한국균학회지
• /
• 제39권2호
• /
• pp.99-104
• /
• 2011

표고버섯의 육종은 두 개의 다른 모균주에 의한 교배와 버섯생산에 좋은 형질을 지닌 교잡균주의 선발이 요구된다. 본 연구에서는 서로 다른 두 계통의 표고 모균주에서 유래한 단핵균주와 이들 단핵균주간의 교배로부터 만들어진 교잡균주에 대하여 발색반응 배지를 이용하여 세포외 분해효소의 분비능력 정도를 비교하여 생화학적 특성이 우수한 균주를 선발할 가능성에 대하여 조사하였다. 모균주로부터 유래한 단핵균주들 간에 ${\beta}$-glucosidase, avicelase, CM-cellulase, amylase, pectinase, xylanase, protease의 분비능력에 차이가 있음을 확인하였다. 교잡균주에 있어서도 단핵균주의 조합에 따라 효소 분비능력이 달라지는 것을 볼 수 있었다. 이에 따라 발색반응배지를 이용한 세포외효소 평가법이 표고의 육종과정 중에 생성되는 교잡균주들의 평가에 활용 할 수 있을 것으로 사료된다.

• 소성∙가공
• /
• 제12권1호
• /
• pp.49-59
• /
• 2003

To reduce the cost of finite element analyses for sheet forming, a 3D hybrid membrane/shell method has been developed to study the springback of anisotropic sheet metals. In the hybrid method, the bending strains and stresses were analytically calculated as post-processing, using incremental shapes of the sheet obtained previously from the membrane finite element analysis. To calculate springback, a shell finite element model was used to unload the final shape of the sheet obtained from the membrane code and the stresses and strains that were calculated analytically. For verification, the hybrid method was applied to predict the springback of a 2036-T4 aluminum square blank formed into a cylindrical cup. The springback predictions obtained with the hybrid method was in good agreement with results obtained using a full shell model to simulate both loading and unloading and the experimentally measured data. The CPU time saving with the hybrid method, over the full shell model, was 75% for the punch stretching problem.

• 한국소성가공학회:학술대회논문집
• /
• 한국소성가공학회 1999년도 춘계학술대회논문집
• /
• pp.62-65
• /
• 1999

To reduce the cost of finite element analyses for sheet forming a 3D hybrid membrance/sheel method has been developed to study the springback of anisotropic sheet metals. in the hybrid method the bending strains and stresses were analytically calculated as post-processing using incremental shapes of the sheet obtained previously from the membrane finite element analysis. To calculate springback a shell finite element model was used to unload the final shape of the sheet obtained from the membran code and the stresses and strains that were calculated analytically. For verification the hybrid method was applied to predict the springback of a 2036-T4 aluminum square blank formed into a cylindrical cup. the springback predictions obtained with the hybrid method was in good agreement with results obtained using a full shell model to simulateboth loading an unloading and the experimentally measured data. The CPU time saving with the hybrid method over the full shell model was 75% for the punch stretching problem.

• 미생물학회지
• /
• 제24권3호
• /
• pp.211-220
• /
• 1986

Nitrogen fixation (nif) genes of Rhizobium leguminosarum were transferred to nif Klebsiella pneumoniae and E. coli by conjugation after partial heat induction of $RP_4$ :: Mu cts in Rhizobium $R^+$ transconjugant, and the hybrid plasmids in the transconjugant strains were isolated and characterized. In order to transfer the nif genes from Rhizobium, the hybrid plasmid $RP_4$ :: Mu cts was transferred by conjugation from E. coil to the symbiotic nitrogen fixer, R. leguminosarum. After stabillity test, the $RP_4$ :: Mu cts in Rhixobium $R^+$ transconjugant was subjected to partial heat induction by culturing it statically at $38^{\circ}C$ for 16 hours, and then conjugated with the nif defective mutant strains of K. pneumoniae or nif mutant strains of E. coli having whole nif gene plasmid. Recombinant strains of K. pneumoniae, which could grow in a N-free medium and exhibit the nitrogenase activity were selected. However, in the case of E. coli, they could grow well in a NA medium containing antibiotices, but hardly frow in a N-free medium. The hybrid plasmids in these transconjugal strains were isolated by gel electrophoresis and compared their molecular sizes.

• Composites Research
• /
• 제13권3호
• /
• pp.21-29
• /
• 2000

The optimum design of hybrid laminated composites for iso-strain structure has been studied by controling fiber orientations and thicknesses of each layer. Fiber orientations and thicknesses of each layer for iso-strain structure were designed. Combining the laminates of each layer of different reinforcing material, the constitutions of hybrid laminated composite for iso-strain structure were obtained. All these calculations were formed on computer systems, automatically for the hybridization. Using the data of some specific laminated composite such as glass and aramid reinforced composites, the constitutions of hybrid laminated composites for iso-strains structure were designed and verified by lamination theory. The strains of each layer of hybrid laminated composites are calculated and they turned out to be good agreements with the results obtained lamination theory.

• 한국잠사곤충학회지
• /
• 제40권1호
• /
• pp.27-32
• /
• 1998

One indigenous polyvoltine strain (Nistari) and two bivoltine strains viz. P5 and NB18 along with one bivoltine hybrid (P5$\times$NB18) were subjected for studies on the differences on some physiologically important biomolecules like protein, nucleic acids and cholesterol. Ovarian protein and RNA content remained significantly high in the bivoltine races and their hybrid over multivoltine breed, while, DNA and cholesterol content remained significantly low in all the breeds and the hybrid as compared to Nistari strain. However, the ovarian weight was higher in both the bivoltine breeds and their hybrid than that of Nistari. Higher ovarian weight together with more protein and RNA concentrations reflect the preparatory phase for production of diapausing eggs by the bivoltine silknoths and their hybrid. The variations in biochemical parameters studied herein, thus establish a distinct difference in the overt reproductive physioiogy between multivoltine and bivoltine silkworms.

• International Journal of Industrial Entomology and Biomaterials
• /
• 제29권2호
• /
• pp.185-192
• /
• 2014

Recombinant proteins can be generated quickly and easily in large amounts and at low-cost in silkworm larvae by using Bombyx mori nuclear polyhedrosis virus (BmNPV). We searched for high-permissive silkworm strains that have high production levels of heterologous proteins and are thus suitable for use as biofactories. In this study, we performed the analysis using a BmNPV vector expressing luciferase as a marker, and we confirmed protein expression by evaluating luciferase activity, determined by western blotting and luciferase ELISA, and confirmed transcription expression by semi- and quantitative real time PCR. For the selection of host silkworm strains, we first chose 52 domestic BmNPV sensitive strains and then identified 10 high-permissive and 5 low-permissive strains. In addition, to determine which hybrid of the high-permissive strains would show heterosis, nine strains derived through three-way crossing were tested for luciferase activity by western blotting, and luciferase ELISA. We found a correlation between luciferase activity and luciferase protein expression, but not transcription. There was no noticeable difference in protein expression levels between Jam313 as the high-permissive control strain and the three-way hybrid strains; however, the three-way cross strains showed lower luciferase activity compared with Jam313. In this study, luciferase protein production in the larvae of 52 domestic silkworm strains was elucidated using BmNPV.