• Korean Journal of Organic Agriculture
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• v.19 no.spc
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• pp.6-8
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• 2011

In order to produce the organic foods in accord with international standard, organic seeds should be used in organic farming. This study was conducted to establish the stable production of organic seed of waxy com by examining the growing characteristics, seed yield, and the economy for seed production by organic farming. The optimal sowing timing for organic seed production of waxy com hybrid was within 10 days of the $1^{st}$ of May with yield of 88~90% of conventional seed production. The optimal planting density was 41,600 plants/ha ($80{\times}30$ cm) for organic seed production of waxy com. The weight of 100 seeds and seed productivity increased at the planting ratio of 2:1 mother plant:male plant. Growth and seed production were improved by removing male plant at 7~10 days after silking. Organic fertilizer (mixed oil cake) was applied at a rate of 4~6 Mg/ha before sowing. Black plastic mulching was used for weed control. In addition, sex pheromone trap and bio-control agents were used for safe pest control and low labour cost.

• Korean Journal of Plant Tissue Culture
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• v.24 no.4
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• pp.239-256
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• 1997

Plants belonging to the category of 2n apomixis or agamospermy form embryos and seeds without the processes of normal meiosis and syngamy. Seeds produced in this way have identical genotype of their maternal parent. Three different types of agamospermy are recognized: diplospory, apospory, and adventitious (adventive) embryony. $F_1$ hybrid cultivars cannot be used as seed sources in the next ($F_2$) generation because this generation would be extremely variable as a result of genetic segregation. Hybrid vigor is also reduced in the $F_2$ generation. Therefore, parental stocks for hybrid seed production need to be maintained and cross must be continuously repeated. Agamospermic 2n apomixis would make it possible to fix the genotype of a superior variety so that clonal seeds faithfully representing that genotype could be continuously and cheaply produced independent of pollination. That is, $F_1$ hybrid seeds could be produced for many generations without loss of vigor or genotype alteration. Production of apomictic $F_1$ hybrid seed would be simplified because line isolation would not be necessary to produce seed or to maintain parental lines, and the use of male-sterile lines could be avoided. Overall, apomixis would enable a significant reduction in hybrid seed production costs. Additionally, the production of clonal seed is not only important for seed propagated crops, but also for the propagation of heterozygous fruit trees and timbers. Clonal seed would help avoid costly and time-consuming vegetative propagating methods that are currently used to ensure the large-scale production of these plants. Apomixis is scattered throughout the plant kingdom, but few important agricultural crops possess this trait Therefore, most research to date has centered on introgressing the trait of apomixis into agricultural crops such as wheat, maize, and some forage grasses from wild distant relatives by traditional cross breeding. The classical breeding approach, however is slow and often impeded by many breeding barriers. These problems could be surmounted by taking mutagenesis or molecular approach. Arabidopsis thaliana is a tiny sexually reproducing plant and is convenient in constructing and screening in molecular researches. Male-sterile mutants of Arabidopsis are particularly suitable genetic background for mutagenesis and screening for apomictic mutants. Molecular approaches towards isolating the genes controlling the apomictic process are feasible. Direct isolation of genes conferring apomixis development would greatly facilitate the transfer of this trait to wide variety of crops. Such studies are now in progress.

• Proceedings of the Korean Society of Crop Science Conference
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• 2022.10a
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• pp.205-205
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• 2022

Sorghum (Sorghum bicolor L.) is a major cereal grain crop of the world and It can be cultivated under high temperature and dry condition with high adaptability to the adverse environment. It is usually eaten with rice in korea and most of domestic sorghum cultivars have been developed by pure line selection and cross breeding. In prior studies, Hybrid cultivar (F1 seed) was known to improve 30-40% in yield and resist to disease and pest than pure line varieties due to hybrid vigor. Therefore, study on the hybrid sorghum is continuously necessary. In korea, hybrid sorghum cultivar have been developed, so we need to find methods seed production technology for supply and commercialization of hybrid seed cultivar. Female inbred line(A-line) and male inbred line(R-line) are needed for hybrid seed production. This study was carried out to investigate growth characteristics and yield of hybrid sorghum according to the seedling period and planting rate between female inbred line(YSA1) and male inbred line (Sodamchal, Miryang 19). When the flowering period of two inbred groups in the process of hybrid seed production is coincides, hybrid seed production is increase. The female inbred line and male inbred lines were cultivated at Daegu in 2021. Two inbred lines were sown at intervals of 3 days and 7days and were evaluated flowering period. As a result, the flowering period of the female inbred line and male inbred lines were matched at the 7days interval. To find out optimal planting rate, two inbred lines were cultivated under different plating rate (4:2, 5:2, 6:2). Yield of YSA1/Sodamchal(F1) was the highest 130(kg/10a) in the 4:2 ratio and yield of YSA1/Miryang 19 was the highest quantity of 139(kg/10a) in the 6:2 ratio. The results of this study could be helpful for hybrid Sorghum seeds production.

• Journal of Forest and Environmental Science
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• v.40 no.3
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• pp.259-264
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• 2024

Dalbergia sissoo and Dalbergia latifolia are the two most important timber wood species that are indigenous to the Indo-Pak subcontinent producing very high-quality timber. Its wood is used to produce high-quality furniture. Due to overexploitation and dieback disease, their production is seriously hampered and threatens their genetic diversity. Several ecotypes are tolerant to dieback thus offering an opportunity to develop a superior hybrid for the establishment of plantations for hybrid seed production. Hybrid evaluation can only be done by using DNA markers such as SSR markers. Cross-specific amplification of SSR markers is a cost-effective way of producing DNA markers for species lacking genetic information. Here, we report for the first-time successful cross-amplification of SSR markers in Dalbergia latifolia and added new SSR markers in D. sissoo. Cross-species amplification resulted in 13 successful SSR markers in D. latifolia and an addition of 14 markers in D. sissoo of expected sizes. Six SSR markers were further selected randomly to validate the breeding behavior of both species. A diverse DNA profile of seed progenies matched to different pollen donors deviated from the same mother suggested cross-pollination is the most likely mechanism of seed production in D. sissoo and D. latifolia. However, the results must be validated by using a large sample size and through controlled pollinations. SSR markers thus developed will be useful in the conservation and development of superior hybrids for sustainable development and production of commercial populations in Dalbergia sissoo and D. latifolia.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.54 no.4
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• pp.346-350
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• 2009

The cultivated radish (Raphanus sativus L.) is a major vegetable crop in the world wide and fast-growing species that grows inhabitats of six continents. It is very important to determine hybrid seed purity in the production of hybrid Brassica vegetable seeds to avoid unacceptable contamination with self-inbred (sib) seeds. The use of random amplified polymorphic DNA (RAPD) markers for evaluating seed purity in $F_2$-hybrid radish cultivars demonstrated. One hundred eighty seeds from the F1 male and female harvest were subsequently screened for seed purity using 13 primers. The 13 primers result in 17 cultivar-specific bands and 23 variable RAPD bands scored for cultivar. RAPD analysis of hybrid seeds from the harvest revealed 128 seeds tested except underdevelopment and decayed seeds were sibs. Especially, $F_2$ hybrids of radish, OPC13, OPD20 were presented clear hybrid bands. It maintains higher than average level of genetic diversity compared with their correspondent parents. RAPD amplification of DNA extracted from germinated individuals from the female harvest reveal that 10 of 208 seeds tested were self-inbred (4.8%). RAPD analysis of hybrid seeds from the male harvest revealed 7 of the 208 seeds tested were sibs (3.4%). The RAPD may lead to a better insight in to the hybrid seed purity.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.48 no.6
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• pp.424-428
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• 2003

This study was conducted to find out the optimum cultural method for hybrid seed production of Chalok 2. Higher seed yield was obtained when KW3 was used as a seed parent (female). KW7 was shown Higher barren stalk occurrence which caused significant seed yield reduction when it was used as a seed parent (female). The ratio of female (KW3) to male (KW7) rows with 4 to 1 had higher seed yield than that of 2:1 or 3:1. In case of using KW3 toy a seed parent, KW3 was susceptible to stem and ear rotting disease. The 35th day after silking was optimal period to harvest due to higher 100-grain weight, lower rotten grain, and higher germination rate.

• Journal of the Korean Society of Tobacco Science
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• v.20 no.2
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• pp.166-171
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• 1998

In order to improve the efficiency of F$_1$ hybrid seeds production(KF 114), some experiments were carried out in a greenhouse this year. Mother plant(MSNC567) and pollen plant(NC) were grown in some pots (30 x 30cm, WxH). The gathered pollens were mixed with celite, pollen deluent dusts and stored in refrigerator at l$0^{\circ}C$ before pollination. To establish the critical range of the mixed rate and the storage period of pollens, the change in a percent of capsule set, number of seed and seed weight per capsule, weight of 1000 seeds and germination percent of seed resulted of pollination with pollen deluent dusts were investigated. The results are as follows; Percent of capsule set showed the mixed rate 1:5 resulted in no difference and those of 1:10 and 1:20 decreased about 5 ～15% when compared with trials using pollen alone. There was no difference among duration of storage in a same mixed rate. Numbers of seed per capsule showed a significant difference among the mixed rates and among durations of storage. Numbers of seed per capsule were decreased about 55 ～ 90 % as the mixed rate increase, about 2 ～ 28 % as the duration of storage increase. Weight of seed per capsule decreased about 16 ～ 23 % as the duration increase and decreased about 3 ～ 89 % as the mixed rate increase when compared with trials using pollen alone. Weight of 1000 seeds showed non significant difference among duration of storage but were higher than trials used pollen alone as the mixed rate increase.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.53 no.4
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• pp.479-485
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• 2020

We evaluated the hatchability of fertilized eggs from six hybrid combinations of highly valued grouper species inhabiting temperate and warm waters, with the goal of establishing a novel hybrid with enhanced growth and viability during the culturing period in the temperate waters of Korea. Hybrid combinations with red-spotted grouper females exhibited high hatchability with high a fertilization and hatching rate of fertilized eggs and a low deformity rate of hatched larvae. Conversely, hybrid combinations with kelp grouper females had very low hatching rates and very high deformity rates; commercial production of seed from such crosses would be difficult without improving hatchability. The hatchabilities of convict grouper ♀×giant grouper ♂ and kelp grouper ♀×red-spotted grouper ♂ were lower than those of maternal purebreds, but these two hybrid combinations were expected to produce potentially large quantities of hatched larvae. In the above evaluation, promising hybrid combinations were identified for commercial production of seed. For these hybrids to contribute to the development of Korea's mariculture industry, mass production of fertilized eggs and seeds is necessary, along with the development of advanced rearing techniques, such as the identification of a suitable rearing temperature.

• Korean Journal of Breeding Science
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• v.41 no.3
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• pp.279-283
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• 2009

A new yellow waxy corn hybrid "Daehakchal Gold 1" was developed from single cross between Yeongdeok Jaera and Okchen Jaera at Chungnam National University in 2007. Inbred CNU57 derived from Yeongdeok Jaera was used as the seed parent of Daehakchal Gold 1, and inbred CNU 27 derived from Okchen Jaera as the pollen parent. Tasseling date of this hybrid was seven day later than that of check hybrid, Chalok 1. Daehakchal Gold 1 was 19.7cm in ear length and 4.5cm in ear diameter. The yield of Daehakchal Gold 1 and check hybrid in dry matter were 146.5g and 113.9g per plant, respectively. On yield trial, which were increased 29.7% compared with a check hybrid, Chalok 1. Especially, Daehakchal Gold 1 had yellow kernels and good eating quality. The ratio of kernel set length/ear length was similar to Chalok 1. It is moderately resistant to Bioporalis maydis and corn borer. The yields of Daehakchal Gold 1 in fresh ear weight and in number of fresh ear were 16% and 8%, respectively, higher than those of a check hybrid in regional yield trials for three years. Seed production of this hybrid has gone well due to good match during crossing between seed and pollen parents.

• Journal of Life Science
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• v.18 no.7
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• pp.907-911
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• 2008

Radish (Raphanus sativus L.) is one of very important crop plants in the world. It is very important to determine hybrid seed quality in the production of hybrid Brassica vegetable seeds to avoid unacceptable contamination with self-inbred (sib) seeds. The allozyme for evaluating seed purity in a commercial $F_1-hybrid$ radish cultivar is demonstrated. Three hundred sixty seeds from the male and female harvest were subsequently screened for seed purity using 27 isozyme loci. Especially, F1 hybrids of radish, Per-1 ($aa{\times}bb$), Lap-1 ($aa{\times}bb$), Est-1 ($aa{\times}bb$) were presented clear hybrid bands. Est-1 locus revealed that 15 (8.3%) seeds from the female harvest and 26 (14.4%) seeds from the male harvest were sibs. It maintains higher than average level of genetic diversity compared with their correspondent parents. Shannon's index of phenotypic diversity (I) of hybrids was the highest of all accessions (R. sativus L. cv. Daepeng, R. sativus L. cv. Backza, and their hybrids). The allozyme may lead to a better insight into the hybrid seed purity.