• 제목/요약/키워드: Hybrid medium model

검색결과 36건 처리시간 0.026초

반응표면분석법을 이용한 Lactobacillus paracasei SRCM201474의 생산배지 최적화 (Application of Response Surface Methodology in Medium Optimization to Improve Lactic Acid Production by Lactobacillus paracasei SRCM201474)

  • 하광수;김진원;임수아;신수진;양희종;정도연
    • 생명과학회지
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    • 제30권6호
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    • pp.522-531
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    • 2020
  • 본 연구는 반응표면분석법을 이용하여 L(+)형 젖산 생산향상을 위한 배지조성을 확립하기 위해 수행되었다. L(+)형 젖산을 선택적으로 고생산하는 것으로 알려진 9종의 Lactobacillus paracasei 균주를 전국에서 수집한 김치 시료로부터 선별하였으며, 젖산 생산량과 glucose로부터의 전환률 분석을 통하여 젖산 생산 배지 최적화를 수행하기 위한 균주로 SRCM201474를 선발하였다. 선택된 11개의 배지 조성 중 젖산 생산에 가장 큰 영향을 미치는 요인을 분석하기 위한 방법으로 Plack-Burman design (PBD)을 설계하였으며, 통계분석을 통해 탄소원으로는 glucose, sucrose, molasses, 질소원으로는 peptone을 최종 선정하였다. 젖산 생산 배지 최적화를 위한 각 변수들의 농도 최적화를 수행하기 위한 방법으로 반응표면분석법 중 적은 실험수로도 최적값을 산출할 수 있는 hybrid design 설계 하였다. 실험 모델에 의한 L. paracasei SRCM201474 균주를 이용한 젖산 생산배지 조성과 최적 농도는 glucose 15.48 g/l, sucrose 16.73 g/l, molasses 39.09 g/l, peptone 34.91 g/l로 나타났으며, 이때의 젖산 생산량은 33.38 g/l로 예측되었다. ANOVA 분석을 통해 가정된 실험 모델의 적합성과 유의성을 확인하였으며, 최종적으로 분석된 최적배지에서의 반복실험을 통한 젖산 생산량을 측정하여 모델에 의해 예측된 젖산생산량과 동일함을 검증하였다. 본 연구를 통해 L(+)형 젖산을 선택적으로 고생산하는 균주를 선발하였으며, 배지 최적화를 수행하여 생분해성 플라스틱 생산을 위한 산업적 젖산 생산에 적용할 수 있는 연구자료로 활용될 수 있을 것으로 판단된다.

Performance Analysis of a Novel Distributed C-ARQ Scheme for IEEE 802.11 Wireless Networks

  • Wang, Fan;Li, Suoping;Dou, Zufang;Hai, Shexiang
    • KSII Transactions on Internet and Information Systems (TIIS)
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    • 제13권7호
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    • pp.3447-3469
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    • 2019
  • It is well-known that the cooperative communication and error control technology can improve the network performance, but most existing cooperative MAC protocols have not focused on how to cope with the contention process caused by cooperation and how to reduce the bad influence of channel packet error rate on the system performance. Inspired by this, this paper first modifies and improves the basic rules of the IEEE 802.11 Medium Access Control (MAC) protocol to optimize the contention among the multi-relay in a cooperative ARQ scheme. Secondly, a hybrid ARQ protocol with soft combining is adopted to make full use of the effective information in the error data packet and hence improve the ability of the receiver to decode the data packet correctly. The closed expressions of network performance including throughput and average packet transmission delay in a saturated network are then analyzed and derived by establishing a dedicated two-dimensional Markov model and solving its steady-state distribution. Finally, the performance evaluation and superiority of the proposed protocol are validated in different representative study cases through MATLAB simulations.

Effect of Parthenogenetic Mouse Embryonic Stem Cell (PmES) in the Mouse Model of Huntington′s Disease

  • 이창현;김용식;이영재;김은영;길광수;정길생;박세필;임진호
    • 한국동물번식학회:학술대회논문집
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    • 한국동물번식학회 2003년도 학술발표대회 발표논문초록집
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    • pp.80-80
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    • 2003
  • Huntington's disease (HD) is an autosomal dominant neurodegenerative disorder characterized by motor, cognitive, and psychiatric symptoms, accompanied by marked cell death in the striatum and cortex. Stereotaxic injection of quinolinic acid (QA) into striatum results in a degeneration of GABAergic neurons and exhibits abnormal motor behaviors typical of the illness. The objective of this study was carried out to obtain basic information about whether parthenogenetic mouse embryonic stem (PmES) cells are suitable for cell replacement therapy of HD. To establish PmES cell lines, hybrid F1 (C57BL/6xCBA/N) mouse oocytes were treated with 7% ethanol for 5 min and cytochalasin-B for 4 hr to initiate spontaneous cleavage. Thus established PmES cells were induced to differentiate using bFGF (20ng/ml) followed by selection of neuronal precursor cells for 8 days in N2 medium. After selection, cells were expanded at the presence of bFGF (20 ng/ml) for another 6 days, then a final differentiation step in N2 medium for 7 days. To establish recipient animal models of HD, young adult mice (7 weeks age ICR mice) were lesioned unilaterally with a stereotaxic injection of QA (60 nM) into the striatum and the rotational behavior of the animals was tested using apomorphine (0.1mg/kg, IP) 7 days after the induction of lesion. Animals rotating more than 120 turns per hour were selected and the differentiated PmES cells (1$\times$10$^4$cells/ul) were implanted into striatum. Four weeks after the graft, immunohistochemical studies revealed the presence of cells reactive to anti-NeuN antibody. However, only a slight improvement of motor behavior was observed. By Nissl staining, cell mass resembling tumor was found at the graft site and near cortex which may explain the slight behavioral improvement. Detailed experiment on cell viability, differentiation and migration explanted in vivo is currently being studied.

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Multistage Inverters Control Using Surface Hysteresis Comparators

  • Menshawi, Menshawi K.;Mekhilef, Saad
    • Journal of Power Electronics
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    • 제13권1호
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    • pp.59-69
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    • 2013
  • An alternative technique to control multilevel inverters with vector approximations has been presented. The innovative control method utilizes specially designed two-dimensional hysteresis comparators to simplify the implementation and improve the resultant waveform. The multistage inverter designed with maximum number of levels is operated in such a way to approximate the reference voltage vector by exploiting the large number of multilevel inverter vectors. A three-stage inverter with the main high voltage stage made of three phase, six-switch and singly-fed inverter is considered for application to the proposed design. The proposed control concept is to maintain a higher voltage stage state as long as it can lead to a target vector. High and medium voltage stages controllers are based on surface hysteresis comparators to hold the switching state or to perform the necessary change to achieve its reference voltage with minimal switching losses. The low voltage stage controller is designed to approximate the target reference voltage to the nearest inverter vector using the nearest integer rounding and adjustment comparators. Model simulation and prototype test results show that the proposed control technique clearly outperforms the previous control methods.

유통업체의 부실예측모형 개선에 관한 연구 (Performance Evaluation and Forecasting Model for Retail Institutions)

  • 김정욱
    • 유통과학연구
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    • 제12권11호
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    • pp.77-83
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    • 2014
  • Purpose - The National Agricultural Cooperative Federation of Korea and National Fisheries Cooperative Federation of Korea have prosecuted both financial and retail businesses. As cooperatives are public institutions and receive government support, their sound management is required by the Financial Supervisory Service in Korea. This is mainly managed by CAEL, which is changed by CAMEL. However, NFFC's business section, managing the finance and retail businesses, is unified and evaluated; the CAEL model has an insufficient classification to evaluate the retail industry. First, there is discrimination power as regards CAEL. Although the retail business sector union can receive a higher rating on a CAEL model, defaults have often been reported. Therefore, a default prediction model is needed to support a CAEL model. As we have the default prediction model using a subdivision of indexes and statistical methods, it can be useful to have a prevention function through the estimation of the retail sector's default probability. Second, separating the difference between the finance and retail business sectors is necessary. Their businesses have different characteristics. Based on various management indexes that have been systematically managed by the National Fisheries Cooperative Federation of Korea, our model predicts retail default, and is better than the CAEL model in its failure prediction because it has various discriminative financial ratios reflecting the retail industry situation. Research design, data, and methodology - The model to predict retail default was presented using logistic analysis. To develop the predictive model, we use the retail financial statements of the NFCF. We consider 93 unions each year from 2006 to 2012 to select confident management indexes. We also adapted the statistical power analysis that is a t-test, logit analysis, AR (accuracy ratio), and AUROC (Area Under Receiver Operating Characteristic) analysis. Finally, through the multivariate logistic model, we show that it is excellent in its discrimination power and higher in its hit ratio for default prediction. We also evaluate its usefulness. Results - The statistical power analysis using the AR (AUROC) method on the short term model shows that the logistic model has excellent discrimination power, with 84.6%. Further, it is higher in its hit ratio for failure (prediction) of total model, at 94%, indicating that it is temporally stable and useful for evaluating the management status of retail institutions. Conclusions - This model is useful for evaluating the management status of retail union institutions. First, subdividing CAEL evaluation is required. The existing CAEL evaluation is underdeveloped, and discrimination power falls. Second, efforts to develop a varied and rational management index are continuously required. An index reflecting retail industry characteristics needs to be developed. However, extending this study will need the following. First, it will require a complementary default model reflecting size differences. Second, in the case of small and medium retail, it will need non-financial information. Therefore, it will be a hybrid default model reflecting financial and non-financial information.

Seismic response of 3D steel buildings with hybrid connections: PRC and FRC

  • Reyes-Salazar, Alfredo;Cervantes-Lugo, Jesus Alberto;Barraza, Arturo Lopez;Bojorquez, Eden;Bojorquez, Juan
    • Steel and Composite Structures
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    • 제22권1호
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    • pp.113-139
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    • 2016
  • The nonlinear seismic responses of steel buildings with perimeter moment resisting frames (PMRF) and interior gravity frames (IGF) are estimated, modeling the interior connections first as perfectly pinned (PPC), and then as partially restrained (PRC). Two 3D steel building models, twenty strong motions and three levels of the PRC rigidity, which are represented by the Richard Model and the Beam Line Theory, are considered. The RUAUMOKO Computer Program is used for the required time history nonlinear dynamic analysis. The responses can be significantly reduced when interior connections are considered as PRC, confirming what observed in experimental investigations. The reduction significantly varies with the strong motion, story, model, structural deformation, response parameter, and location of the structural element. The reduction is larger for global than for local response parameters; average reductions larger than 30% are observed for shears and displacements while they are about 20% for bending moments. The reduction is much larger for medium- than for low-rise buildings indicating a considerable influence of the structural complexity. It can be concluded that, the effect of the dissipated energy at PRC should not be neglected. Even for connections with relative small stiffness, which are usually idealized as PPC, the reduction can be significant. Thus, PRC can be used at IGF of steel buildings with PMRF to get more economical construction, to reduce the seismic response and to make steel building more seismic load tolerant. Much more research is needed to consider other aspects of the problem to reach more general conclusions.

하이브리드 인공신경망 모형을 이용한 부도 유형 예측 (Bankruptcy Type Prediction Using A Hybrid Artificial Neural Networks Model)

  • 조남옥;김현정;신경식
    • 지능정보연구
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    • 제21권3호
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    • pp.79-99
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    • 2015
  • 부도 예측은 회계와 재무 분야에서 꾸준히 연구되고 있는 분야이다. 초기에는 주로 다중판별분석(multiple discriminant analysis)와 로짓 분석(logit analysis)과 같은 통계적 방법을 이용하였으나, 1990년대 이후에는 경영 분야의 분류 문제를 위해 많은 연구자들이 인공신경망(back-propagation neural network), 사계기반추론(case-based reasoning), 서포트 벡터 머신(support vector machine) 등과 같은 인공지능을 통한 접근법을 이용하여 통계적 방법보다 분류 성과 측면에서 우수함을 입증해왔다. 기존의 기업의 부도에 관한 연구에서 많은 연구자들이 재무비율을 이용하여 부도 예측 모형을 구축하는 것에 초점을 맞추어왔다. 부도예측에 관한 연구가 꾸준히 진행되고 있는 반면, 부도의 세부적인 유형을 예측하여 제시하는 것에 대한 연구는 미흡한 실정이었다. 따라서 본 연구에서는 수익성, 안정성, 활동성 지표를 중심으로 국내 비외감 건설업 기업들의 부도 여부뿐만 아니라 부도의 세부적인 유형까지 예측 가능한 모형을 개발하고자 한다. 본 연구에서는 부도 유형을 예측하기 위해 두 개의 인공신경망 모형을 결합한 하이브리드 접근법을 제안하였다. 첫 번째 인공신경망 모형은 부도예측을 위한 역전파 인공신경망을 이용한 모형이며, 두 번째 인공신경망 모형은 부도 데이터를 몇 개의 유형으로 분류하는 자기조직화지도(self-organizing map)을 이용한 모형이다. 실험 결과를 통해 정의된 5개의 부도 유형인 심각한 부도(severe bankruptcy), 안정성 부족(lack of stability), 활동성 부족(lack of activity), 수익성 부족(lack of profitability), 회생 가능한 부도(recoverable bankruptcy)는 재무 비율에 따라 유형별로 상이한 특성을 갖는 것을 확인할 수 있었다. 본 연구 결과를 통해 신용 평가 분야의 연구자와 실무자들이 기업의 부도의 유형에 대한 유용한 정보를 얻을 것으로 기대한다.

18FDG Synthesis and Supply: a Journey from Existing Centralized to Future Decentralized Models

  • uz Zaman, Maseeh;Fatima, Nosheen;Sajjad, Zafar;Zaman, Unaiza;Tahseen, Rabia;Zaman, Areeba
    • Asian Pacific Journal of Cancer Prevention
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    • 제15권23호
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    • pp.10057-10059
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    • 2015
  • Positron emission tomography (PET) as the functional component of current hybrid imaging (like PET/CT or PET/MRI) seems to dominate the horizon of medical imaging in coming decades. $^{18}$Flourodeoxyglucose ($^{18}FDG$) is the most commonly used probe in oncology and also in cardiology and neurology around the globe. However, the major capital cost and exorbitant running expenditure of low to medium energy cyclotrons (about 20 MeV) and radiochemistry units are the seminal reasons of low number of cyclotrons but mushroom growth pattern of PET scanners. This fact and longer half-life of $^{18}F$ (110 minutes) have paved the path of a centralized model in which $^{18}FDG$ is produced by commercial PET radiopharmacies and the finished product (multi-dose vial with tungsten shielding) is dispensed to customers having only PET scanners. This indeed reduced the cost but has limitations of dependence upon timely arrival of daily shipments as delay caused by any reason results in cancellation or rescheduling of the PET procedures. In recent years, industry and academia have taken a step forward by producing low energy, table top cyclotrons with compact and automated radiochemistry units (Lab-on-Chip). This decentralized strategy enables the users to produce on-demand doses of PET probe themselves at reasonably low cost using an automated and user-friendly technology. This technological development would indeed provide a real impetus to the availability of complete set up of PET based molecular imaging at an affordable cost to the developing countries.

실리카 나노 입자에 결합된 항종양제: 악성종양 치료를 위한 새로운 치료 방법 (Antitumor agents bound to silica nanoparticles: potential technology for the remediation of malignant tumors)

  • 이영환;이정옥;전경수
    • 분석과학
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    • 제23권6호
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    • pp.579-586
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    • 2010
  • 현재 상업적으로 널리 사용 되여 지고 있는 항암제인 hydroxy urea, 6-mercaptopurine monohydrate, cytosine arabinoside, cyclophosphamide monohydrate 그리고 uracil를 3-(triethoxysilyl) propyl isocyanate 와 반응시켜 항암제가 붙어있는 3-(triethoxysilyl)propyl amide (compound I)을 합성한후 물과 가수분해 반응시켜 항암제가 결합된 silica 나노입자(10 nm~micronparticles)를 만들 수있었다. Silyl isocyanate 유도체들은 물과 반응하여 유기물질-silica 나노입자가 포함된 $-CH_2-CH_2-CH_2-NH-COOH$ 그룹이나 혹은 온도와 용매등 반응조건에 따라서 decarboxylated 된 propylamine 그룹이 생성되었다. 생체외 시험에서 항암제가 결합된 silica 나노입자는 종양 세포 제거에 효과적이고 정상세포에 거의 영향을 미치지 않는 것으로 나타났다. 그리고 악성 종양인 폐, 난소, 악성 흑색종, 중추신경계(CNS)와 결장 종양 세포가 이 연구에 사용되었다. 현재의 연구에서 전달매체 로서 silica는 silation 반응으로 손쉽게 나노입자를 얻을 수 있으므로 본 연구에 쉽게 이용 할 수 있다. 결과로부터 이 기술은 보다 부작용이 적은 생체 의약품에 적합한 carrier nanoparticles에 적용 될 수 있을 것으로 판단된다.

북극해에서 입자추적 방법을 이용한 유빙 추적 연구 (Tracing the Drift Ice Using the Particle Tracking Method in the Arctic Ocean)

  • 박광섭;김현철;이태희;손영백
    • 대한원격탐사학회지
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    • 제34권6_2호
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    • pp.1299-1310
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    • 2018
  • 본 연구는 북극해에 분포하는 유빙의 움직임을 이해하기 위해 현장관측 자료와 입자 추적 방법을 사용하여 분포 및 이동경향을 분석하였다. 북극해에서 유빙의 움직임은 NOAA(National Oceanic and Atmospheric Administration)에서 제공하는 ITP(Ice-Tethered Profiler)의 자료 중에서 2009년부터 2018년 자료를 이용했다. 유빙의 유동은 각 연도별로 분류하고 각각의 ITP 자료를 이용하여 위치 및 속도를 분석하였다. 입자 추적은 HYCOM(Hybrid Coordinate Ocean Model)과 ECMWF(European Centre for Medium-Range Weather Forecasts)에서 제공하는 일별 해류 및 바람 자료를 사용하여 2009년부터 2018년까지의 유빙의 움직임을 모의하였다. 북극해 전역에서 유빙의 이동경향을 분석하기 위해서 현장관측 자료인 ITP자료를 입력 자료로 이용하여 북극해에서 해류와 바람과의 관계식을 계산하여 라그랑지안 입자 추적을 수행하였다. 입자 추적 시뮬레이션은 해류에 의한, 그리고 해류와 바람에 의한 영향을 고려한 두 종류의 실험을 수행하였고, 대부분의 입자는 해류와 바람의 영향을 고려한 경우에 현장관측 자료와 동일하게 재현되었다. 북극해에서 유빙의 움직임은 바람의 영향을 고려한 관계식을 이용하여 재현되었고, 이를 이용하여 특정 연도의 유빙의 이동경향을 분석하였다. 2010년의 경우 Arctic Oscillation Index(AOI)는 음의 해로 입자들은 보퍼트 환류(Beaufort Gyre)를 따라 명확하게 움직임을 보이고, 극점 인근에서는 상대적으로 더 빠른 속도를 나타낸다. 반면에 2017년의 경우 AOI는 양의 해로 대부분의 입자들은 Gyre에 크게 영향을 받지 않는 움직임을 보이며 보퍼트 해 (Beaufort Sea) 인근에서 나타나는 이동속도 또한 상대적으로 감소하였고, 극점에서의 이동속도도 감소했다. 2010년과 2017년의 계절적 특징은 2010년도의 유빙의 이동속도는 동계(0.22 m/s)에 증가되고 춘계(0.16 m/s)에 감소되며, 2017년의 경우 하계(0.22 m/s)에 증가되고 춘계(0.13 m/s)에 감소되었다. 결과적으로 입자추적 방법은 제한된 현장관측 자료를 대신하여 북극해에서 유빙의 분포 및 이동경향을 이해할 수 있는 방법으로 위성자료와 연계하여 장기적인 유빙의 탐지 및 이동경향을 이해하는 유용한 방법이 될 것이다.