• Journal of Chest Surgery
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• v.32 no.1
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• pp.38-42
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• 1999

Background: In neonatal respiratory distress syndrome patients, various types of pulmonary air leak contributes to elevate morbity and mortality. Although early surgical interventions can provide better results in several cases, whole clinical outcomes are poor. This study was designed to investigate the clinical aspects of pulmonary air leak in the neonatal respiratory distress syndrome patients and major contributing factors to mortality. Material and Method : We retrospectively evaluated 48 cases of pulmonary air leak in the neonatal respiratory distress syndrome patients from September 1994 to May 1997. Result: There were 15 cases of primary and 33 cases of secondary pulmonary air leakages. The prominent manifestations were pure interstitial emphysema in 19 cases(39.9%) and combined pneumothorax in 19 cases(39.9%). In clinical aspects, normal birth weight was dominant(83.4%), the onset occurred within 24 hours was in 28 cases(58.8%). The pulmonary diseases were meconium aspiration syndrome(25.2%) and hyaline membrane disease(33.2%). The overall hospital mortality was 25.2%, and the majority were hyaline membrane diseases. Conclusion: Although the overral mortality rate of these diseases were high, more detailed studies about immediate treatement, perinatal prevention, intensive care to geriatric problems were needed individually to improve outcomes.

• The Korean Journal of Nuclear Medicine
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• v.6 no.2
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• pp.7-20
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• 1972

Experimental radiation nephritis was produced in 15 rabbits by X-irradiation. About $2,000{\gamma}$ (tissue doses) were given to both kidneys of a rabbit in 5 days. Other tissues and organs except both kidneys were protected with 2mm thickened lead plates. 5 weeks after the last irradiation, blood pictures, blood pressures, B.U.N., serum creatinine, Ca, Mg, Fe levels and serum erythropoietin activity of the irradiated rabbits were studied. After finishing above studies, rabbits were sacrificed and both kidneys were removed and examined histopathologically. Same laboratory and pathological studies were performed in 6 control rabbits. In this study, the author obtained following results. 1) Both kidneys of rabbitis with experimental raditation nephritis showed marked histopathological changes, i.e.: renal tubules showed diffuse cloudy swelling, impacted intraluminal hyaline casts and focal precipitations of lime salts on the tubular epithelium. Diffuse interstitial fatty necrosis and various degrees of fibrotic infiltrations on the interstitium were also seen in association with focal lymphocytic infiltrations. Hyaline degenerations were observed on the glomeruli and small vessels. 2) Experimental radiation nephritis rabbits showed marked lowering in R.B.C. counts, decreased hemoglobin levels, low hematocrit values and leucopenia in comparison with those of control rabbits. (P<0.01). (Table 1 & 2). 3) Mild proteinuria were observed in experimental radiation nephritis in rabbits. 4) The levels of B.U.N. and serum creatinine increased in experimental radiation nephritis. (P<0.01). (Table 1,3 & 4) 5) The levels of serum Ca and Mg Showed no statistical difference in comparison with those of control rabbits. (P>0.05) (Table 3 & 4). 6) No statistical correlations were observable between the levels of B.U.N. and Hb. values. (${\gamma}=-0.223$) No close correlations (${\gamma}=-0.328$) were noticed between the levels of B.U.N. and serum iron levels. 7) Erythropoietin activity (R.B.C. $^{59}Fe$ Incorporation) was measured by the modified Fried method. No change in its activity was noticed in radiation nephritis group comparing with that of the control group. (P>0.05). (Table 1,3 & 5). 8) Carotid artery blood pressures showed also no difference. (P>0.05). (Table 1 & 2).

• Applied Microscopy
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• v.36 no.4
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• pp.259-269
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• 2006

Hemocytes of the spider Araneus ventricosus were investigated with histochemistry, density analysis of percoll gradient, and fine structural examinations using transmission electron microscope. The hemocytes of this spider were classified into two major groups: granulocytes and non-granulocytes. The granular hemocytes were subdivided into three subtypes according to their histochemical properties which are eosinophilic granuloctes(EGs), basophilic granulocytes(BGs) and cyanocytes. The EGs, which have small granules within the cytoplasm comprise about 5% of the total henocytes. However the granules of BGs are larger than those of HGs. The cyanocytes were characterized to contain hemocyanin granules in their cytoplasm. On the other hand, the non-granulocytes were divided into three subtypes; hyaline leucocytes, oenocytoids, and molting homocytes. The hyaline leucocytes are the most abundant and the smallest hemocyte type in this spider. The oenocytoids that have $10{\sim}15{\mu}m$ in diameter are mostly found at the marginal region of the myocardium in the heart tube. The molting hemocytes, which only appeared during the molting period, contains plenty of glycogen particles in their cytoplasm.

• The Korean Journal of Cytopathology
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• v.19 no.1
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• pp.41-46
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• 2008

In an attempt to better define the cytologic characteristics of schwannomas, we have reviewed aspirates and corresponding histologic sections from 23 schwannomas. Of this number, the original cytologic diagnoses were: schwannoma in 14 cases (61%), benign soft tissue tumor in 2 cases (9%), and insufficient specimen in 7 cases (30%). The cytologic findings common to all cases of schwannoma included fragments of tightly cohesive fascicles with variable cellularity and corresponding Antoni type A area. The Antoni type B area, consisting of scattered spindle cells and some histiocytes and lymphocytes against a myxoid background, was seen in 14 cases. Fibrillary stroma was seen in 12 cases. The tumor cells had spindle- or ovalshaped nuclei, with pointed ends and indistinct cell borders. Nuclear palisading was seen in 10 cases, and distinctive Verocay bodies were seen in 5 cases. In ancient schwannomas, there were no Verocay bodies. Most schwannomas have distinct cytomorphologic features that allow correct diagnosis. The major problem with fine needle aspiration cytology of these tumors is the high frequency of poor cellularity, particularly in lesions with cystic degeneration. Of 7 cases with insufficient specimen, 4 showed marked cystic changes and 1 showed marked hyaline changes on histologic sections. In conclusion, we believe that if cytopathologist reminds the situation such as cystic degeneration or hyaline degeneration, the correct diagnosis of the schwannoma will be easily made.

• Archives of Plastic Surgery
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• v.37 no.6
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• pp.815-818
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• 2010

Purpose: Soft tissue chondroma is a rare benign tumor, found mainly on the palm and sole and grows slowly. Typically, mature hyaline cartilage is the dominant pathological feature. There are reports that assert soft tissue chondromas to be a cause of median nerve entrapment syndrome. However, this is the first case report showing soft tissue chondroma to be a cause of simultaneous median and ulnar neuropathy. Methods: A 62 year-old woman presented with chief complaints of numbness and hypoesthesia of her right palm for 4 to 5 years, and a palpable mass on her right palm that had been increasing in size slowly for 3 years. Physical examination revealed a firm, mobile, non-tender and about $3{\times}3\;cm^2$ sized mass in the center of the right palm. Electromyography showed entrapment neuropathy of the median and ulnar nerve. Ultrasonography showed an approximately $5.7\;cm^2$ mass below the flexor tendon of ring finger. Upon surgical excision, a $3{\times}3\;cm^2$ mass attached to the flexor digitorum profundus of ring finger and redness and hypertrophy of both the median and ulnar nerve were discovered. Mass excision was performed gently and the specimen was referred for histopathologic study. Mass excision resulted in median and ulnar nerve release. Results: The pathology report confirmed the mass to be a soft tissue chondroma with mature hyaline cartilage. The patient exhibited post-operative improvement of her symptoms and did not show any complications. Conclusion: This is the first case report showing soft tissue chondroma to be a cause of simultaneous median and ulnar neuropathy.

• Korean Journal Plant Pathology
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• v.14 no.2
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• pp.109-114
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• 1998

To investigate Phomopsis species causing fruit decays of Japanese apricot, apple and kiwifruit, we collected diseased fruits from the fruit markets in 1995 and 1996 respectively. Phomopsis mali Roberts was identified based on cultural characteristics, morphological aspects and pathogenicity. There were no remarkable differences with respect to $\alpha$ and $\beta$ conidia, growth rates and colony characters among the isolates from Japanese apricot, apple and kiwifruit. The pathogens grew more than 70 mm on potato dextrose agar in 5 days at $25^{\circ}C$. The agar was slightly discolored by the production of a reddish purple pigment under the light at $25^{\circ}C$ and 3$0^{\circ}C$ respectively. Only $\alpha$ spores of the different isolates of P. mali were formed at 15$^{\circ}C$ and $\beta$ spores were mainly produced at 3$0^{\circ}C$, but and $\alpha$ and $\beta$ spores were produced in approximately equal numbers at 2$0^{\circ}C$ and $25^{\circ}C$. Pycnidia were a few under the dark condition but were abundant at wide range of 15~3$0^{\circ}C$ under near ultra violet illumination. Conidia were two types : $\alpha$ spores were unicellar, fusoid, hyaline and biguttulate, whereas $\beta$ sores were unicellar, acicular to filiform, straight or hooked and hyaline. An ascigerous stage was not formed in cultures or in nature. Isolates of Phomopsis mali from japanese apricot, apple and kiwifruit could infect fruits of apple, pear, apricot, Japanese apricot and kiwifruit. There were some differences in pathogenicity depending on stocks of fruit crops tested.

• ALGAE
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• v.35 no.4
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• pp.303-324
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• 2020

Cocconeis coreana and C. sijunghoensis are described as new based on micromorphological and molecular data. C. coreana is represented by five unialgal cultures from four different freshwater bodies, two from North Korea and three from South Korea. C. sijunghoensis is represented by two unialgal cultures from a brackish water body in North Korea. Except for one, all of the strains auxosporulated and showed an almost quadrupling of size in length and width. Morphologically, these species with their two different elliptical valves belong to the Cocconeis placentula group. The raphe valve has striae with uniseriate areolae continuing across a pronounced submarginal hyaline rim to the edge of the valve. The sternum valve has uniseriate dash-like areolae continuously from the valve face until the valve edge. Micromorphologically, these species possess two different open valvocopulae: only the raphe valvocopula has fimbriae; the sternum valvocopula has none. Based on p-distances of currently available DNA sequence data, i.e., rbcL and 18SV4, both species are pronouncedly different from the epitype strain of C. placentula, with C. coreana closest to the published molecular data of the strain UTEX FD23 named C. placentula from Iowa, USA, while C. sijunghoensis is closest but not the same as the published molecular data of strain D36_012, the epitype strain of C. placentula from Berlin, Germany. Based on scanning electron microscope observations, differentiating features are discussed concerning valvocopula fimbriae, central area, areolation of the sternum valve and on the raphe valve especially between the submarginal hyaline rim and edge.

• Journal of Biomedical Engineering Research
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• v.23 no.2
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• pp.147-153
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• 2002

The purpose of this study was to evacuate the effect of different types of Poly(lactic-co-glycolic acid) (PLGA) scaffolds on the formation of human auricular and septal cartilages. All of the scaffolds were formed in a tubular shape for potential application for artificial trachea or esophagus with either 110,000 g/mol PLGA. 220,000 g/mol PLGA. or a combination of both. In order to maintain the tubular shape in vivo, two methods were used. One method was inserting polyethylene tube at the center of scaffolds made of 110,000 g/mol PLGA. The other method involved combination of the two different molecular weight PLGA's. The inner surface of tubular shaped scaffold made with 110,000 g/mol PLGA was coated with 220,000 9/mol PLGA to give more mechanical rigidity. Elastic cartilage was taken from the ear of a patient aged under 20 nears old and hyaline cartilage was taken from the nasal septum. The chondrocytes were then isolated. After second passage, the chondrocytes were seeded on the PLGA scaffolds followed by in vitro culture for one week. The cells-PLGA scaffold complex were implanted subcutaneously on the back of nude mice for 8 weeks. The tissue engineered cartilages were separated from nude mice and examined histologically after staining with the Hematoxylin Eosin. The morphology of the scaffolds were examined by scanning electron microscopy. The pores were well formed and uniformly distributed in the various PLGA scaffolds. After 8 weeks in vivo culture, cartilage was well formed with 110,000 g/mol PLGA. however lumen had collapsed. In contrast. a minimal amount of neocartilage was formed with 220,000 g/mol PLGA, while the architecture of scaffold and lumen were well preserved. Elastic cartilage formed more neocartilage than hyaline. Hyaline and elastic neocartilage were well formed on 110,000 g/mol PLGA with the polyethylene tube, exhibiting mature chondrocytes and preservation of the tubular shape. It was found that 110,000 g/mol PLGA was more appropriate for cartilage formation but higher molecular weight polymer was necessary to maintain the three dimensional shape of the scaffold.

• The Plant Pathology Journal
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• v.15 no.4
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• pp.236-241
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• 1999

Sclerotinia twig blight on trees and cottony rot on fruits of Satsuma mandarin were observed in Cheju, Korea in 1996 and 1997. The causal fungus causing citrus twig blight and cottony rot was identified as Sclerotinia sclerotiorum which had cup-shaped apothecia bearing hyaline and clavate asci and periphyses on sclerotium. Symptoms were produced on twigs, developing young leaves, fully developed new leaves, and fruits 2-9 days after wound inoculation, but only on twigs with young lateral buds and developing young leaves by unwound inoculation. The fungus grew well between 10 and $27^{\circ}$, but optimum temperature was $24^{\circ}$ on potato dextrose agar. Most varieties were highly susceptible to S. sclerotiorum, whereas some varieties such as Ichinan were relatively resistant among twelve Satsuma mandarin varieties in the field. This is the first record of Scledrotinia twig blight on trees and cottony rot on stored fruits of Satsuma mandarin in Korea.

• The Plant Pathology Journal
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• v.15 no.4
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• pp.242-246
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• 1999

Gloeocercospora sorghi caused the zonate leaf spot on sorghum (Sorghum bicolor Moench) in fields in Korea. The zonate spots were conspicuous on sorghum leaves as circular, reddish purple bands alternating with tan or straw-colored areas, which formed a coarsely zonate pattern. The lesions often occurred in semicircular patterns along the margin of leaves. The fungus produced sporodochia on the surface of infected leaves in a moist chamber. The conidia, born in a pinkish to salmon-colored slimy matrix, were hyaline, elongate to filiform, straight or slightly curved, 3- to 17-septate, and of variable lenght (28-197 x 1.6-$3.4\mu\textrm{m}$). G. sorghi isolated from diseased leaves of sorghum caused characteristic reddish-brown water-soaked leaf spots when inoculated to seedlings.