• Journal of Plant Biology
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• v.18 no.2
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• pp.59-85
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• 1975

With a view to obtaining basic informations on the morphological, physiological and ecological aspects of hop in Korea, this experiments were conducted in 4 locations (Suwon, Hongchun, Pyungchang and Hoengsung) from 1972 to 1974. The varieties used in this experiments were Hallertau, Saaz, Shinshuwase and Cascade. the results obtained are summarized as follows.

• Korean Journal of Plant Resources
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• v.37 no.4
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• pp.431-438
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• 2024

Hops enhance beer flavor and bitterness, acting as a preservative. In recent years, the booming trend of craft beer has prompted the introduction of foreign hop varieties for cultivation and production in South Korea. This study focuses on developing efficient in vitro culture condition of the hop (Humulus lupulus L.) variety 'Cascade' for treatments of plant growth regulators, i.e. IAA and Cytokinin. Using Auxin IAA and Cytokinin 2iP, Zeatin, BAP, and TDZ on MS medium as plant growth regulators, the experiment involved removing three nodes from the shoot apex. In vitro hop culture showed the highest shoot proliferation rate when only IAA was added, with approximately 21% higher compared to the combination with Cytokinin. Notably, IAA 0.1 mg/L + BAP 1 mg/L resulted in a superior shoot proliferation rate of around 91%. IAA 0.1 mg/L + BAP 1 mg/L was advantageous for shoot elongation. Callus induction occurred with TDZ, while control or IAA-only conditions exhibited shoot and root growth. Cytokinin addition led to callus formation and increased weight. Assessing survival and soil adaptation during in vitro hop seedling acclimatization involved maintaining near 100% humidity initially, gradually reducing it over three weeks. When transferred outdoors, 9 out of 10 seedlings acclimated successfully, confirming a 90% acclimatization rate.

• Korean Journal of Pharmacognosy
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• v.24 no.3
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• pp.231-234
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• 1993

Neuropharmacological profile of Humulus lupulus (hop) extract was studied in mice. At doses above 100 mg/kg(i.p.), it decreased spontaneous locomotor activity and raised the nociceptive threshold in the hot-plate test. At doses above 250 mg/kg (i.p.), it increased pentobarbital-induced sleeping time and produced muscle relaxant effect. At the dose of 500 mg/kg, anticonvulsive effect against pentylenetetrazole-induced convulsion and hypothermic effect was observed.

• Natural Product Sciences
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• v.18 no.4
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• pp.261-265
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• 2012

Xanthohumol was isolated from hops (Humulus lupulus L.), and then investigated anti-oxidant effect by AAPH-induced LLC-PK1 cell and oxygen radical absorbance capacity (ORCA) assays and MMP-12 inhibitory effect by direct MMP-12 inhibition assay. The treatment of xanthohumol protected LLC-PK1 cells from AAPH-induced cell damage such as cell viability, SOD and GSH-px reduction in a dose dependant manner (0.1, 1, and $5{\mu}M$), the SOD value was 2.98, 4.51, and 5.77 U/mg protein, and GSH-px value was 30.12, 49.32, and 60.11 U/mg protein. ORAC value of xanthohumol was showed as 4320, 12004, and $14209{\mu}M$ TE/g at the concentration 0.1, 1, and $5{\mu}M$, respectively. The change of SOD and GSH-px values was significantly correlated with the results of ORAC assay, that is, AAPH-induced cell and ORCA assays. In addition, inhibition of MMP-12 that is known to play an important role in skin aging was 14%, 37%, 46%, and 79% at the concentration of 0.01, 0.1, 1, and $5{\mu}M$, respectively. On the basis of these results, xanthohumol from hops (Humulus lupulus L.) showed interesting biological and pharmacological activity such as anti-oxidant effect and anti-aging.

• Korean Journal of Pharmacognosy
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• v.49 no.3
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• pp.189-202
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• 2018

Hop cones (Humuli Strobili) are the female inflorescences of hop plants (Humulus lupulus L.) belonging to the family Cannabaceae. They have been used as herbal remedies to treat mood and sleep disturbances, and mainly to add as a bittering ingredient in brewing process. Considerable interests on pharmacological and biological activities of hop cones have been focused on their major constituents, namely, phloroglucinols (humulone, lupulone), terpenes (myrcene, humulene), and prenylated flavonoids (xanthohumol, isoxanthohumol, 6-prenylnaringenin, and 8-prenylnaringenin). The present review describes and discusses biological activity profiles of these major compounds in the hop cones.

• Proceedings of the Korean Society of Crop Science Conference
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• 1988.07a
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• pp.50-51
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• 1988

• Proceedings of the Korean Society of Crop Science Conference
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• 1988.07a
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• pp.52-53
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• 1988

• Nutrition Research and Practice
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• v.6 no.5
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• pp.405-413
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• 2012

Rho iso-alpha acids-rich extract (RIAA) from Humulus lupulus (hops) and proanthocyanidins-rich extracts (PAC) from Acacia nilotica exert anti-inflammatory and anti-diabetic activity in vitro and in vivo. We hypothesized that a combination of these two extracts would exert enhanced effects in vitro on inflammatory markers and insulin signaling, and on nonfasting glucose and insulin in db/db mice. Over 49 tested combinations, RIAA:PAC at 5:1 ($6.25{\mu}g/mL$) exhibited the greatest reductions in $TNF{\alpha}$-stimulated lipolysis and IL-6 release in 3T3-L1 adipocytes, comparable to $5{\mu}g/mL$ troglitazone. Pretreatment of 3T3-L1 adipocytes with this combination ($5{\mu}g/mL$) also led to a 3-fold increase in insulin-stimulated glucose uptake that was comparable to $5{\mu}g/mL$ pioglitazone or $901{\mu}g/mL$ aspirin. Finally, db/db mice fed with RIAA:PAC at 5:1 (100 mg/kg) for 7 days resulted in 22% decrease in nonfasting glucose and 19% decrease in insulin that was comparable to 0.5 mg/kg rosiglitazone and better than 100 mg/kg metformin. RIAA:PAC mixture may have the potential to be an alternative when conventional therapy is undesirable or ineffective, and future research exploring its long-term clinical application is warranted.

• Korean Journal of Plant Resources
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• v.36 no.2
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• pp.181-190
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• 2023

Domestic hop (Humulus lupulus L.) production has been suspended since the early 1990s due to foreign imports, but interest in local production is rising due to the recent craft beer boom in Korea. This study was conducted focusing on the development of growth characteristics and propagation technology for 6 introduced hop cultivars as a basic study for domestic hop production and breeding program. In the hop growth survey conducted in 2021 and 2022, the 5-year-old plants after planting generally showed a tendency to increase the height of strobile setting, strobile size, number and weight of strobile per hill compared to the 4-year-old plants. As a result of the experiment with hop vine cuttings, the average rooting rate of all cultivars was as high as 88% even in only water treatment that were not added with Atonik (Atonik, Arysta, Japan), a rooting agent. There were differences between cultivars in rooting length and rooting rate according to the Atonik treatment method. When checking the survival rate of the rooted cuttings seedlings after transplanting into the soil, it was confirmed that the survival rate of the cuttings in the tissue culture room was significantly lower than that of the cuttings in the greenhouse. However, in transplanting step, cutting plants from culture room condition was strongly inhibited plant growth because of changing environment conditions. As a results of tissue culture, the thidiazuron (TDZ) 1 ㎎/L treatment in the media generated 6 to 9 shoots/explant, while the 6-benzylaminopurine (BAP) 1 ㎎/L treatment generated only 1 to 2 shoots/explant. Therefore, it is more effective to culture by adding TDZ rather than BAP. These results indicated that the development of technology to manage stably after transplanting of cutting or micropropagating plants into potting soil is important for mass propagation of hops.

• Proceedings of the Korean Society of Toxicology Conference
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• 2001.10a
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• pp.35-36
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• 2001

Nutrition influences cancer incidence and offers a variety of preventive dietary factors including non-nutritive plant metabolites. To identify novel potential chemopreventive agents, we have set up cell- and enzyme-based in vitro marker systems relevant for prevention of carcinogenesis in vivo. This experimental approach led to the identification of Xanthohumol (Xn), a prenylated chalcone from hop (Humulus lupulus L.) as a most promising broad-spectrum chemopreventive agent.(omitted)