• 대한약학회:학술대회논문집
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• 대한약학회 2001년도 Proceedings of the Pharmaceutical Society of Korea
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• pp.217.1-217.1
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• 2001

• 대한약학회:학술대회논문집
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• 대한약학회 2001년도 Proceedings of the Pharmaceutical Society of Korea
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• pp.218.2-218.2
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• 2001

• 대한약학회:학술대회논문집
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• 대한약학회 2000년도 춘계총회 및 학술대회
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• pp.218.1-218.1
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• 2000

• 대한약학회:학술대회논문집
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• 대한약학회 2002년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.1
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• pp.219.1-219.1
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• 2002

• Bulletin of the Korean Chemical Society
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• 제34권12호
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• pp.3817-3824
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• 2013

Various separation modes in HPLC, such as anion exchange (AE), reversed-phase (RP), and affinity (AF) chromatography were examined for the separation of selenium species in human blood serum and urine. While RP- and AE-HPLC were mainly used for the separation of small molecular selenium species, double column AF-HPLC achieved the separation of selenoproteins in blood serum efficiently. Further, the effluent of AF-HPLC was enzymatically hydrolyzed and then analyzed with RP HPLC for selenoamino acid study. The versatility of the hybrid technique makes the in-depth study of selenium species possible. For quantification, post column isotope dilution (ID) with $^{78}Se$ spike was performed. ORC ICP/MS (octapole reaction cell inductively coupled plasma/mass spectrometry) was used with 4 mL $min^{-1}$ Hydrogen as reaction gas. In urine sample, inorganic selenium and SeCys were identified. In blood serum, selenoproteins GPx, SelP and SeAlb were detected and quantified. The concentration for GPx, SelP and SeAlb was $22.8{\pm}3.4\;ng\;g^{-1}$, $45.2{\pm}1.7\;ng\;g^{-1}$, and $16.1{\pm}2.2\;ng\;g^{-1}$, respectively when $^{80}Se/^{78}Se$ was used. The sum of these selenoproteins ($84.1{\pm}4.4\;ng\;g^{-1}$) agrees well with the total selenium concentration measured with the ID method of $87.0{\pm}3.0\;ng\;g^{-1}$. Enzymatic hydrolysis of each selenium proteins revealed that SeCys is the major amino acid for all three proteins and SeMet is contained in SeAlb only.

• 분석과학
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• 제15권6호
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• pp.529-533
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• 2002

광학이성질체 분리 컬럼, 시료처리 및 농축 컬럼이 부착된 컬럼스위칭-HPLC를 이용하여 뇨시료에서 미량의 플루비프로펜 광학이성질체를 분리 정량하는 방법을 연구하였다. 분리된 각 이성질체의 고유광회전도를 구하고, d-이성질체 및 l-이성질체를 확인하였다. 이들의 검정선의 농도범위는 각각 $0.11{\sim}5.4{\mu}g/mL$이었으며, 검출한계는 d-이성질체의 경우 $0.027{\mu}g/mL$이고, l-이성질체의 경우 $0.031{\mu}g/mL$이며, 날자간 및 날자내의 정밀도의 CV값은 각각 1.84% 이하였다. 본 분석법은 플루비프로펜 약물을 복용한 살마의 뇨중에 존재하는 플루비프로펜의 분석에 적용하였다.

• Journal of Yeungnam Medical Science
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• 제4권2호
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• pp.105-111
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• 1987

본 연구에서 우리는 음식물 특히 철판구이 쇠고기를 섭취한 후 배설된 사람뇨에서 돌연변이 유발능 물질의 존재유무를 관찰하고저 하였다. Ames씨 방법에 따라 2종(TA 98, TA 100)의 Salmonella typhimurium을 이용하였으며, 흡연 자체에 의해 야기될 수 있는 돌연변이 유발능을 배제하기 위하여 비흡연자인 건강한 두 남녀의 소변을 사용하였다. 300g의 철판구이 쇠고기를 먹은 후 배설된 뇨의 돌연변이 유발능은 남여 모두에서 약 3시간 후부터 검출되기 시작하여 6시간 후 가장 높았다가 그후 감소하는 경향을 관찰 할 수 있었다.

• 대한의용생체공학회:의공학회지
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• 제28권4호
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• pp.577-584
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• 2007

Uroflowmetry is non-invasive and easily performed to diagnose benign prostatic hypertrophy(BPH) frequent in aged men. Weight change during urination is usually measured to estimate the urinary flow rate by a load cell, but sensitive to any impacts against the bottom of the container, leading to unnecessary noise generation. Moreover, load cells are relatively expensive raising the production cost. The present study proposed a new technique, measuring hydraulic pressure on the bottom of the urine container to evaluate the urinary volume. Low cost pressure transducer enabled almost perfectly linear relationship between the urine volume and the hydraulic pressure. During both the simulated and human urination experiments, variance of the pressure signal was more than 50% smaller than the weight signal acquired by a load cell, which demonstrated that the impact noise was decreased to a great degree by pressure compared to weight measurement.

• 한국산업보건학회지
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• 제26권4호
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• pp.396-403
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• 2016

Objectives: The study is to evaluate biological monitoring and risk assessment for epichlorohydrin-bisphenol A resin exposed from waterproofing or finishing work in the apartment building construction. Methods: Subjected workers were working on spray-painting and waterproofing work for 8 hours per day every 20 days. The urine samples were collected at the end of 20 days working period. For urinary bisphenol A as metabolite from epichlorohydrin-bisphenol A exposure, urine samples were analyzed with liquid chromatography mass-mass spectrometry(HPLC-MS/MS). Results: Geometric means of urinary bisphenol A(BPA) with no hydrolysis and with enzymic hydrolysis(BPA-EH) in the workers were $1.10{\mu}g/L$ and $2.90{\mu}g/L$. BPA-EH was 4 times higher than that of control group. The factors for working period and ages did not affect the variation of BPA and BPA-EH. The levels for BPA and BPA-EH were not higher than 95th percentile for exposure on human-life environment. Conclusions: The BPA and BPA-EH were therefore effective biological markers for epichlorohydrin-bisphenol A exposure workers, but not seem to hazardous exposure level. Waterproofing work in construction workshop is required to measuring work environment and health care management for the workers.

• 분석과학
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• 제15권3호
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• pp.185-189
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• 2002

Cyclofenil은 국제올림픽위원회(IOC)에서 금지약물로 규정하고 있다. 이에 따라 본 실험에서는 GC/MS를 사용하여 소변으로부터 cyclofenil의 복용여부를 검사할 수 있는 방법을 만들었다. 이를 위하여 cyclofenil 및 그 대사체들의 추출 회수율을 측정하였는데 pH 5-9 사이가 최적의 추출 조건이었으며, 소변으로는 모 약물인 cyclofenil은 배설되지 않아서 검출하지 못하였고 대신에 그 대사체들을 검출함으로써 cyclofenil의 복용여부를 검출할 수 있었다. 따라서 대사체들은 가수분해 후 pH 9.6에서 에테르로 추출하여 MSTFA로 유도체화시켜서 GC/MS로 분석하였다.